• Title/Summary/Keyword: Non-mixture

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Atomospheric Concentration and Mutagenicity of Nitroarenes of Suspended Particulates in Seoul (대기부유분진중 nitroarenes 오염도와 그 돌연변이원성에 관한 조사연구 - 서울시 일부지역을 중심으로 -)

  • Jo, Seong-Joon;Choi, Yong-Wook;Lim, Young-Wook;Shin, Dong-Chun;Chung, Yong
    • Environmental Analysis Health and Toxicology
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    • v.5 no.3_4
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    • pp.19-28
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    • 1990
  • Nitroarenes are ubiquitous environmental pollutants displaying potent mutagenicity in bacteria and carcinogenicity in mammal. In this study, the concentration of nitroarenes in coarse and fine particles and mutagenicity of POC$\_$N/ fraction was investigated in suspended particulates at the Shinchon and Bulkwang area of Seoul. The suspended particulates were collected bimonthly by a high volume cascade impactor air sampler from July 1987 to May 1988. Extractable organic matter was obtained by ultrasonic extraction on diethly ether/cyclohexane (8/2, v/v). Neutral fraction was obtained by liquid-liquid extraction. Polar neutral organic compounds (POC$\_$N/) was fractionated by thin-layer chromatography. Finally, the concentrations of nitroarenes in POC$\_$N/ fraction were measured and determined by capillary gas chromatography. Direct and indirect mutagenicity of POC$\_$N/ fraction were measured using Salmonella typhimurium TA 98. The result were as follows: 1) Major nitroarenes at the Shinchon area was 1-nitropyrene and at the Bulkwang area it was 2,7-dinitro-9-fluorenone during the year. 2) Average concentration of total nitroarenes measured was 67.26 ng/m$^3$in fine particles which was 1,3 folds higher that in coarse particle (52.30 ng/m$^3$). 3) Annual pattern of nitroarenes concentrations revealed that concentration during heating season (Feb., Jan., Mar.) was 2.2 folds higher than that in non heating season (May, Jul., Sep.). Concentration of each season has 157.68 ng/m$^3$and 80.39 ng/m$^3$. 4) The mutagenic activity of POC$\_$N/ fraction from fine particles was higher compared to that of coarse particles and was increased when metabolically activated, with 59 mixture. Mutagenicities, Metabolically activated, were significantly different between Shinchon and Bulkwang area, 322.8 rev/250 $\mu\textrm{g}$/plate and 286.8 rev/250 $\mu\textrm{g}$/plate, respectively. 5) Annual pattern of mutagenicity of POC$\_$N/ fraction revealed that mutagenicity during the heating season was 1.7 folds higher at Shinchon area and 1.2 folds higher at Bulkwang than during the non heating season. The variable contents and levels of nitroarenes in suspended particulates may affect human health significantly. Further studies such as risk assessment should be conducted on the basis of these kind of studies.

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Expression of Pigments in Black Rice during Kernel Development (흑미의 종자 발달에 따른 색소발현 양상)

  • Lee, Jeom-Sig;Jang, Jae-Ki;Chun, A-Reum;Choung, Myoung-Gun;Kim, Hye-Won;Oh, Sea-Kwan;Lee, Jeong-Heui;Yoon, Mi-Ra;Kim, Dae-Jung;Song, You-Chun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.2
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    • pp.127-131
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    • 2012
  • In this study we examined the changes in expression of pigments in black rice during kernel development, which were sampled at 2~3-day intervals to the 40th day after flowering. The first expression of pigment on kernels was observed on the seed coat about 5 days after flowering. At that times, the ratio of pigment expression was 0.08% of total area. The order in expression of pigments in black rice during kernel development was top first, followed by bottom, dorsal side, then ventral side. Maximum percentage of the total colored area in kernel was about 25 days after flowering. After that, the color has changed to dark purple from pale purple during kernel development after flowering. After harvesting, the non-uniform color kernels were observed. As a result, the ventral side in a kernel was a position of the non-uniform color such as a mixture of pale purple and dark purple. Also, we could be concluded that patten of pigment expression was similar in kernel development.

Environment Friendly Control of Gray Mold, a Ginseng Storage Disease Using Essential Oils (정유를 이용한 환경친화적 수삼 저장병 방제)

  • Kim, Jung-Bae;Kim, Nam-Kyu;Lim, Jin-Ha;Kim, Sun-Ick;Kim, Hyun-Ho;Song, Jeong-Young;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.15 no.3
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    • pp.236-241
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    • 2009
  • The objective of this study was to find an environment friendly method of ginseng storage disease control using a natural plant extract. Essential oil was evaluated in terms of its antifungal ability against a variety of ginseng storage pathogens, and a variety of essential oils was conducted in order to assess the possibility of applying them as a component of a disease control strategy. Direct treatment with essential oil was demonstrated to exert a ginseng storage control effect. Methyl eugenol and thymol were shown to exert a mycelial growth inhibition effect of 80% on PDA media, using a paper disc containing 200 ppm of essential oil against Botrytis cinerea. The application of direct methyl eugenol treatment to ginseng resulted in a profound control effect. Both spray and dipping treatment of each methyl eugenol as well as thymol, evidenced a disease develoment of 10-20% as compared with the over 80% observed from all non-treated packages. Methyl eugenol in the large packages resulted in a disease index of 0.60 in the two essential oil treatments and also a small diseased area, as compared with the disease index of 1.65 and the wide diseased area observed in the non-treatment groups. Treatment with a mixture (methyl eugenol + thymol) in the synergistic effect test resulted in a relatively wide diseased area, as no discernable synergistic effect was detected. Methyl eugenol and thymol can be utilized as control agents in an environmentally friendly ginseng storage treatment, owing to the avirulent and clear effects detected in this study. In particular, ginseng must be ingested when fresh, and this is why a product for the control of ginseng storage diseases is so necessary.

The crystallization behavior of glass made from coal bottom ash (석탄 바닥재로 제조된 유리의 결정화 거동 분석)

  • Jang, Seok-Joo;Kang, Seung-Gu
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.20 no.1
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    • pp.58-63
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    • 2010
  • The glass-ceramics made from the mixture of coal bottom ash, produced from a thermal power plant mixed with $Na_2O$ and $Li_2O$ was fabricated and their crystallization behavior was studied using a non-isothermal analyzing method. The temperature for 50% crystallization was higher than the exothermic peak temperature $T_p$ at DTA curve and the quickest crystallization temperature was much the same as $T_p$ as identified from the relationships of crystallized fraction and crystallization rate with temperature. By using Kissinger equation describing a crystallization behavior, the activation energy (262 kJ/mol), the Avrami constant (1.7) and the frequency ($5.7{\times}10^{16}/s$) for crystallization were calculated from which the nepheline crystal could be expected as showing an 1~2-dimensional surface crystallization behavior mainly with some bulk crystallization tendency at the same time. The actual observation of microstructure using SEM showed the considerable amount of surface crystals of dendrite and the bulk crystals with low fraction, so the prediction by the Kissinger equation was in accord with the crystallization behavior of glass-ceramics fabricated in this study.

Experiment and Simulation of 2-bed PSA for Hydrogen Separation from H2/CH4 Gas Mixture (수소/메탄 혼합 기체로부터 수소 분리를 위한 두 탑 PSA 실험과 전산 모사)

  • Nam, Gi-Moon;Jeong, Byung-Man;Kang, Seok-Hyun;Lee, Chang-Ha;Lee, Byung-Kwon;Choi, Dae-Ki
    • Korean Chemical Engineering Research
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    • v.43 no.2
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    • pp.249-258
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    • 2005
  • A two-column six-step pressure swing adsorption(PSA) process was to study separation of hydrogen from hydrogen and methane($60_{vol}%/40_{vol}%$) binary system onto activated carbon adsorbent. The effects of the feed gas pressure, the feed flowrate and the P/F(purge to feed) ratio on the process performance were evaluated. The cyclic steady-states of PSA process were reached to after 15 cycles. $H_2$ purity increases according as the P/F ratio and pressure increase and the feed flow rate decreases; however, $H_2$ recovery shows an opposite phenomena to the purity. PSA process simulation studied to find optimum operation condition. In the results, 22 LPM feed flowrate, 11 atm adsorption pressure and 0.10 P/F ratio might be optimal values to obtain more than 75% recovery and 99% purity hydrogen. In this study was non-isothermal and non-adiabatic model considering linear driving force(LDF) model and Langmuir-Freundlich adsorption isotherm considered to compare between prediction and experimental data.

Kinetic study on Low-rank Coal Including K2CO3, Na2CO3, CaCO3 and Dolomite Gasification under CO2 Atmosphere (이산화탄소 분위기에서 K2CO3, Na2CO3, CaCO3 및 Dolomite가 첨가된 저급탄의 가스화에 대한 반응특성연구)

  • Hwang, Soon Choel;Kim, Sang Kyum;Park, Ji Yun;Lee, Do Kyun;Lee, Si Hyun;Rhee, Young Woo
    • Clean Technology
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    • v.20 no.1
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    • pp.64-71
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    • 2014
  • We have investigated the effects of various additives on Eco coal gasification under $CO_2$ atmosphere. The temperature ranges from $750{\sim}900^{\circ}C$ and the gasification experimental was carried out with Eco coal adding 7 wt% $K_2CO_3$, $Na_2CO_3$, $CaCO_3$, Dolomite, and non-additive under $N_2$ and $CO_2$ gas mixture. At $850^{\circ}C$, we observed that the reaction rate increased when the concentration of $CO_2$ increased. However, we also observed that the increment of reaction rate was small at more than 70% of the concentration of $CO_2$. The additives activity was ranked as 7 wt% $Na_2CO_3$ > 7 wt% $K_2CO_3$ > non-additive > 7 wt% Dolomite > 7 wt% $CaCO_3$ at $850^{\circ}C$. At the temperatures of $750^{\circ}C$, $800^{\circ}C$, $850^{\circ}C$, and $900^{\circ}C$, when the temperature increased, the gasification rate increased. The gasification was suitably described by the volumetric reaction model. Using volumetric reaction model, the activation energy of Eco coal including 7 wt% $Na_2CO_3$ gasification was 83 kJ/mol, which was the lowest value among all the alkaline additives.

Effects of DBD-bio-plasma on the HSP70 of Fibroblasts: A New Approach on Change of Molecular Level by Heat Shock in the Cell (Fibroblasts 세포주의 HSP70에 대한 DBD-bio-plasma의 effects: Cell에서 Heat Shock에 의한 Molecular Level 변화로의 새로운 접근법)

  • Kim, Kyoung-Yeon;Yi, Junyeong;Nam, Min-Kyung;Choi, Eun Ha;Rhim, Hyangshuk
    • KSBB Journal
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    • v.30 no.1
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    • pp.21-26
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    • 2015
  • Plasma is an ionized gas mixture, consisting of neutral particles, positive ions, negative electrons, electronically excited atoms and molecules, radicals, UV photons, and various reactive species. Also, plasma has unique physical properties distinct from gases, liquids, and solids. Until now, non-thermal plasmas have been widely utilized in bio-medical applications (called bio-plasma) and have been developed for the plasma-related devices that are used in the medical field. Although numerous bio-plasma studies have been performed in biomedicine, there is no confirmation of the nonthermal effect induced by bio-plasma. Standardization of the biological application of plasma has not been evaluated at the molecular level in living cells. In this context, we investigated the biological effect of bio-plasma on living cells. Hence, we treated the fibroblasts with Dielectric Bauvier Discharge bio-plasma (DBD), and assessed the characteristic change at the molecular level, one of the typical cellular responses. Heat shock protein 70 (HSP70) regulates its own protein level in response to stimuli. HSP70 responds to heat shock by increasing its own expression at the molecular level in cells. Hence, we confirmed the level of HSP70 after treatment of mouse embryonic fibroblasts (MEFs) with DBD. Interestingly, DBD-plasma induced cell death, but there was no difference in the level of HSP70, which is induced by heat shock stimuli, in DBD-treated MEFs. Our data provide the basic information on the interaction between MEFs and DBD, and can help to design a molecular approach in this field.

Code for Unplanned Encounters at Sea(CUES): Its Limitation and Recommendations for Improvement (해상에서의 우발적 조우 시 신호 규칙(CUES)의 제한점과 개선을 위한 제언)

  • Oh, Dongkeon
    • Strategy21
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    • s.44
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    • pp.323-351
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    • 2018
  • Adopted in Western Pacific Naval Symposium(WPNS) 2014, Code for Unplanned Encounters at Sea(CUES) has been the most valuable output of WPNS history. Written and suggested by Australian Navy in 1999, the goal of CUES is to decrease the possibility of the naval conflict by establishing the code among international navies in the Western Pacific region. Facing many oppositions and requirement of People's Liberation Army Navy(PLAN) in WPNS 2012 and 2013, but it finally adopted in WPNS 2014, with many changes in detailed provisions. From then, navies in the Western Pacific region have followed CUES to prevent maritime conflicts in the region, CUES, however, sometimes does not work correctly. Contents of CUES is the mixture of the parts of Multinational Maritime Tactical Signal and Maneuvering Book(MTP) and International Regulations for Preventing Collision at Sea 1972(CORLEGs). There are means of radio communications such as frequency and signals, instructions for maneuvering and so on. Thus, it is not a new document for the U.S. Navy and its allies, but it requires training to implicate at sea for navies other than U.S. allies, like PLAN. Lots of provisions in CUES were changed because of the opposition of PLAN, and CUES has many shortcomings and practical limitations. First, since CUES is non-legally binding, and there are no methods to force the naval assets on the sea to follow. Second, CUES is only applied to naval assets; naval ships - warships, naval auxiliaries, and submarines - and naval aircraft. Third, the geographical scope in CUES is not clear. Fourth, there is no provision for submerged submarines. Finally, CUES has no time-based framework or roadmap for training. In this regard, there would be six recommendations for improvement. First, CUES should be reviewed by WPNS or other international institutions, while keeping non-binding status so that WPNS could send signals to the navies which do not answer CUES on the sea. Second, the participation of Maritime Law Enforcements(MLEs) such as coast guard is inevitable. Third, navies would use full text of MTP rather than current CUES, which extracts some parts of MTP. Fourth, CUES needs provisions with respect to submerged submarines, which recognizes as offensive weapons themselves. Fifth, the geographic scope of CUES should be clear. Since there are some countries in which claim that a rock with a concrete structure is their territory, CUES should be applied on every sea including EEZ and territorial seas. Finally, the detailed training plan is required to implicate CUES at sea. Rim of the Pacific (RIMPAC) is a good exercise to train CUES, because almost all WPNS member countries except six countries are participating in RIMPAC. CUES is a meaningful document not only for navies but also for nation-states in the region. To prevent escalation of conflict in the region, potentially caused by an unplanned collision at sea, CUES should be applied more strictly. CUES will continue to be in subsequent WPNS and therefore continue to improve in the effectiveness as both an operational and diplomatic agreement.

Mobilization of Heavy Metals Induced by Button Mushroom Compost in Sunflower

  • Lee, Jong-Jin;Lee, Heon-Hak;Kim, Sung-Chul;Yoo, Jeoung-Ah;Lee, Chan-Jung;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.469-476
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    • 2015
  • Button mushroom compost (BMC) was prepared by fermenting the mixture of waste button mushroom bed collected from Boryeong area in South Korea (4): sawdust (8) : pig and fowl manure (1) for 40 days at $30^{\circ}C$. The BMC compromised diverse microorganisms including aerobic bacteria $8.1{\times}10^6cfu\;g^{-1}$, Gram negative bacteria $1.7{\times}10^7cfu\;g^{-1}$, genus Bacillus $6.4{\times}10^6cfu\;g^{-1}$, genus Pseudomonas $1.5{\times}10^4cfu\;g^{-1}$, actinomycetes $1.0{\times}10^4cfu\;g^{-1}$, and fungi $3.5{\times}10^3cfu\;g^{-1}$. BMC was used as a microbial inoculant for estimating the mobilization of heavy metals in soil or plant. When metal solubilization potential of BMC was assessed in a batch experiment, the inoculation of BMC was shown to increase the concentrations of water soluble Co, Pb, Cd, and Zn by 29, 26, 27, and 43% respectively, than those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 17, 15, 18, and 21% respectively in Co, Pb, Cd, and Zn contaminated soils. Moreover, enhanced accumulation of Co, Pb, Cd, and Zn in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the BMC. The apparent results suggested that the BMC could effectively be employed in enhancing phytoextraction from the soils contaminated with heavy metals such as Co, Pb, Cd, and Zn.

A mixture of blackberry leaf and fruit extracts decreases fat deposition in HepG2 cells, modifying the gut microbiome

  • Wu, Xuangao;Jin, Bo Ram;Yang, Hye Jeong;Kim, Min Jung;Park, Sunmin
    • Journal of Applied Biological Chemistry
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    • v.62 no.3
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    • pp.229-237
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    • 2019
  • More effective treatments are needed for non-alcoholic fatty liver disease (NAFLD). We hypothesized that water extracts of blackberry fruits (BF) and leaves (BL) and their combinations (BFL) reduce fat deposition in HepG2 cells and modulate shor-tchain fatty acids (SCFA) and fecal bacteria in vitro. HepG2 cells were treated with BF, BL, BFL1:2, and BFL1:3 for 1 h, and 0.5 mM palmitate was added to the cells. Moreover, low ($30{\mu}g/mL$) and high doses ($90{\mu}g/mL$) of BL and BF were applied to fecal bacteria in vitro, and SCFA was measured by GC. BL, BF, BFL1:2, and BFL1:3 reduced triglyceride deposition in the cells in a dose-dependent manner, and BFL1:2 and BFL1:3 had a stronger effect than BF. The content of malondialdehyde, an index of oxidative stress, was also reduced in BL, BF, and BFL1:2 with increasing superoxide dismutase and glutathione peroxidase activities. The mRNA expression of acetyl CoA carboxylase, fatty acid synthase, and sterol regulatory element-binding protein-1c was reduced in BL, BF, BFL1:2, and BFL1:3 compared to the control, and BFL1:2 had the strongest effect. By contrast, the carnitine palmitolytransferase-1expression, a regulator of fatty acid oxidation, increased mostly in BFL1:2 and BFL1:3. Tumor necrosis factor-${\alpha}$ and interleukin-$1{\beta}$ expression was reduced in BL compared to that in BF and BFL1:2 in HepG2 cells. Interestingly, BL increased propionate production, and BF increased butyrate and propionate production and increased total SCFA content in fecal incubation. BF increased the contents of Bifidobacteriales and Lactobacillales and decreased those of Clostridiales, whereas BL elevated the contents of Bacteroidales and decreased those of Enterobacteriales. In conclusion, BFL1:2 and BFL1:3 may be potential therapeutic candidates for NAFLD.