• BMB Reports
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• v.31 no.1
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• pp.25-30
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• 1998

Kinetic studies of L-Alanine dehydrogenase from Bacillus subtilis-catalyzed reactions in the presence of $Zn^{2+}$ were carried out. The substrate (L-alanine) saturation curve is hyperbolic in the absence of the metal ion but it becomes sigmoidal when $Zn^{2+}$ is added to the reaction mixture indicating the positive cooperative binding of the substrate in the presence of zinc ion. The cooperativity of substrate binding depends on the xinc ion concentration: the Hill coefficients ($n_H$) varied from 1.0 to 1.95 when the zinc ion concentration varied from 0 to $60\;{\mu}m$. The inhibition of AlaDH by $Zn^{2+}$ is reversible and noncompetitive with respect to $NAD^+$ ($K_i\;=\;5.28{\times}10^{-5}\;M$). $Zn^{2+}$ itself binds to AlaDH with positive cooperativity and the cooperativity is independent of substrate concentration. The Hill coefficients of substrate biding in the presence of $Zn^{2+}$ are not affected by the enzyme concentration indicating that $Zn^{2+}$ binding does not change the polymerization-depolymerization equilibria of the enzyme. Among other metal ions, $Zn^{2+}$ appears to be a specific reversible inhibitor inducing conformational change through the intersubunit interaction. These results indicate that $Zn^{2+}$ is an allosteric competitive inhibitor and substrate being a non-cooperative per se, excludes the $Zn^{2+}$ from its binding site and thus exhibits positive cooperativity. The allosteric mechanism of AlaDh from Bacillus subtilis is consistent with both MWC and Koshland's allosteric model.

• Wind and Structures
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• v.25 no.2
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• pp.131-156
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• 2017

This paper deals with the dynamic stability of embedded functionally graded (FG)-carbon nanotubes (CNTs)-reinforced micro cylindrical shells. The structure is subjected to harmonic non-uniform temperature distribution and 2D magnetic field. The CNT reinforcement is either uniformly distributed or FG along the thickness direction where the effective properties of nano-composite structure are estimated through Mixture low. The viscoelastic properties of structure are captured based on the Kelvin-Voigt theory. The surrounding viscoelastic medium is considered nonhomogeneous with the spring, orthotropic shear and damper constants. The material properties of cylindrical shell and the viscoelastic medium constants are assumed temperature-dependent. The first order shear deformation theory (FSDT) or Mindlin theory in conjunction with Hamilton's principle is utilized for deriving the motion equations where the size effects are considered based on Eringen's nonlocal theory. Based on differential quadrature (DQ) and Bolotin methods, the dynamic instability region (DIR) of structure is obtained for different boundary conditions. The effects of different parameters such as volume percent and distribution type of CNTs, mode number, viscoelastic medium type, temperature, boundary conditions, magnetic field, nonlocal parameter and structural damping constant are shown on the DIR of system. Numerical results indicate that the FGX distribution of CNTs is better than other considered cases. In addition, considering structural damping of system reduces the resonance frequency.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.200-205
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• 2001

The effects of dietary supplementation with the sodium salt of isobutyric acid in a low protein (10% CP) wheat straw based diet on nutrient utilization, blood metabolites and growth performance were studied with male crossbred calves. The calves were divided into two equal groups of 6 each. The animals of the control group were fed a basal diet consisting of wheat straw, concentrate mixture and green oat fodder in 40:40:20 proportion whereas BCFA supplemented group received the basal diet+isobutyric acid at 0.75 percent of basal diet. The duration of study was 120 days. The feed intake between experimental groups did not differ significantly and the average total DMI (% BW) was 1.99 and $1.95kg\;day^{-1}$ in control and BCFA supplemented diets. The dietary supplementation of BCFA improved (p<0.01) the DM, OM, CP (p<0.05), NDF and cellulose digestibilities by 8.50, 9.01, 5.39, 17.78 and 18.44 per cent over those fed control diet. The total N retention on BCFA supplementation was improved (p<0.01) due to the decreased (p<0.05) faecal N excretion. The BCFA supplementation did not alter the blood circulatory levels of glucose, total protein, albumin, urea N and amino acids. However after 120 days of experimental feeding a significant (p<0.05) increase in the concentrations of non-esterified fatty acid was observed in control group. The DCP intake and the DCP content of experimental diets was similar in both groups. However, the TDN content of BCFA supplemented diet was significantly (p<0.01) higher (64.35%) than that of control (59.60%). The total live weight gain in BCFA supplemented diet increased by 15.94% over control. The average daily gain and efficiency of feed conversion were also improved in BCFA fed calves by 13.38 and 26.71% respectively, compared to control. It is concluded that dietary supplementation with isobutyric acid improved the digestibility of nutrients and growth performance of calves.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.10
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• pp.1405-1408
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• 2001

Ca salt of soybean oil (PSO) and that of mustard oil plus mahua oil (PMOMO) (50:50) were prepared using double decomposition method, and further tested for their fatty acid composition and degree of saponification. Furthermore, the different levels of protected fat of PSO and PMOMO were evaluated in wheat straw based total mixed ration (TMR) in vitro. Results indicated that capric, lauric, myristic, palmitic, steric, oleic, linoleic, leinolenic acids were traces, traces, traces, 10.00, 2.00, 25.00, 58.50, 5.0% in PSO while the corresponding values in PMOMO were 1.08, 0.28, 0.45, 16.9, 12.95, 44.38, 17.46 and 6.50%, respectively. The degree of saponification of both protected fat supplements was more than 80%. Six treatment combinations were tested I.e., blank without feed and fat supplement (T1); control diet with out fat supplement (T2); control diet plus bypass fat supplement (PSO) so that diet contain 5% fat (T3); control diet plus bypass fat supplement (PSO) so that diet contain 7.5% fat (T4); two more diets viz. T5 and T6 were formulated using bypass fat supplement from PMOMO containing 5 and 7.5% fat respectively. TMR was prepared using 50% concentrate mixture and 50% wheat straw. Result indicated that TVFA, $NH_3-N$,TCA-N, total-N and total gas production were increased in treatment diets at 7.5% level of supplementation, however, fermentation pattern remain similar at 5.0% level of supplementation with respect to control diet. Nevertheless, IVDMD and IVOMD values remained unchanged, rather non-significant at both fat levels and with the both fat sources. On the basis of results it was concluded that Ca-salt of Soybean oil or Mustard plus Mahua oil did not show any negative effect either on digestibility or on microbial protein synthesis in rumen, hence the dietary fat upto 7.5% level in total mixed ration based on wheat straw, could be safely used without any adverse effect on rumen fermentation.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.825-830
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• 2006

Status of blood minerals and their absorption by neonate calves as influenced by fat soluble vitamins supplementation in their respective mothers, mineral supplementation in calves themselves has been evaluated. The objective was to know the impact of antioxidant vitamin supplementation to advance pregnant buffaloes, on enhanced acquired immunity during first few hours after birth, in relation to weight gain in buffalo calves. Advance pregnant buffaloes (n = 30) consisting of average body weight of $550{\pm}15$ kg and of 4-6 parity were fed on 25 kg green (green Jawar-Sorghum bicolor), 2-3 kg wheat straw and 3-4 kg concentrate mixture individually per day. Intramuscular injections of vitamin triplex A $D_3$ E consisting of -2,500,000 IU of vit A -Palmitate; 2,500,000 IU of vitamin $D_3$ and 1,000 IU of vit E (dl-alpha tocopherol acetate) were given per dose, a month prior to parturition, twice at 15 days interval to 15 dams. Rest of the 15 pregnant buffaloes served as negative controls. Secretion of immune proteins, immunoglobulin (Ig) enhanced by 80% in colostrum. The blood serum levels of Zn, Cu, Ca, Mg were measured from birth to 90 days in calves. A significant (p<0.05) difference between the blood serum Zn levels of calves born to vitamin supplemented and non-supplemented dams was measured and a positive correlation between blood serum Zn levels and injections of vitamins was identified. Association of Zn and Cu with passive immunity status has been identified in these calves. A significant positive correlation between Zn and Cu was also identified which showed a change under the impact of vitamin supplementation in buffaloes. The study signifies the role of micronutrients supplementation in dams prior to parturition, in calf immunity development. The study indicates significant mineral - vitamins interactions during this process.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.47-53
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• 1997

Appropriation of partial substitution of concentrate mixture by urea-molasses-mineral block (UMMB) lick supplements for 20 lactating crossbred cows in 2nd and 3nd lactation was studied. Animals fed on wheat straw ad lib. and Berseem (Trifolium alaxandrium) fodder @ 1.5 kg/d on dry matter basis. Animals of control group were given concentrate supplement, while in treatment groups 10% of the concentrate requirement was substituted with 3 different types of UMMB lick type A ($T_1$), type B ($T_2$) and type C ($T_3$). CP content of the ration was 15%. Total dry matter intake (DMI) was about 1.0 kg/kg of fat corrected milk (FCM) yield and was not significantly different between control and treatment groups. Digestibility of neither proximate principles nor cell wall constituents were deviated on UMMB licks partial supplementation. FCM yield was increased by 140, 410 and 460 g/d, in $T_1$, $T_2$ and $T_3$, respectively, in comparison to control group but differences were statistically invalid. Though fat per cent was reduced, fat yields were remain constant among treatments. Milk composition was unaltered except significant difference (p < 0.01) in non-protein nitrogen (NPN) content. Gross-N and digestible-N conversion was significantly higher (p < 0.01) with $T_1$, $T_2$ and $T_3$ than control group. Energy utilization efficiency for milk production was only 36%. Result demonstrated that UMMB licks could be partial supplemented up to 10% of the concentrate requirement of crossbred cows yielding on an average 14kg/d without any adverse effect on feed intake, nutrient utilization and mild production. Comparatively, UMMB lick type B and C was proved better than type A and also economically viable.

• The Journal of the Korea Contents Association
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• v.13 no.2
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• pp.100-107
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• 2013

Agn$\grave{e}$s Varda is a French representative female film director. In particular, the method combining subjectivity and objectivity is the most outstanding characteristics. However, it can be said that the method to support this is not only theme of the film but also creation of structure including the exploration for the filmic form different from classical film form. Such an approach accords with No$\ddot{e}$l Burch's refined analysis of filmic form. Therefore, This study aims to aesthetical analysis of the form of producing the structure in modern movies through which is one of the representative work by Agn$\grave{e}$sVarda and won the Golden Lion award in 1985 Venice Film Festival. The theme of the film, the recovery of relationships among people and contacts through the tragic death of drifting life, created a new filmic structure by formative experiment of the film. The formative experiment is the fragmented and repetitive construction with the introductory voice-over, and consists of movement and editing of camera and specific use of flashback and sound through the representation of figures and situations, mixture of narrative and non-narrative style.

• The Journal of the Acoustical Society of Korea
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• v.15 no.2
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• pp.40-48
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• 1996

In this paper, a 40 dimensional segment vector with 4 frame and 7 frame width in every monosyllable interval was compressed into a 10, 14, 20 dimensional vector using K-L expansion and neural networks, and these was used to speech recognition feature parameter for CHMM. And we also compared them with CHMM added as feature parameter to the discrete duration time, the regression coefficients and the mixture distribution. In recognition test at 100 monosyllable, recognition rates of CHMM +${\bigtriangleup}$MCEP, CHMM +MIX and CHMM +DD respectively improve 1.4%, 2.36% and 2.78% over 85.19% of CHMM. And those using vector compressed by K-L expansion are less than MCEP + ${\bigtriangleup}$MCEP but those using K-L + MCEP, K-L + ${\bigtriangleup}$MCEP are almost same. Neural networks reflect more the speech dynamic variety than K-L expansion because they use the sigmoid function for the non-linear transform. Recognition rates using vector compressed by neural networks are higher than those using of K-L expansion and other methods.

• Journal of Bio-Environment Control
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• v.13 no.3
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• pp.125-129
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• 2004

This study was conducted to identify the growth characteristics of melon cultivated with mixture medium of scoria and perlite in capillary system. The growth and fruit characteristics of all cultivars cultivated under the nutrient solution capillary supplying system were comprehensively favorable. Fresh fruit weight of the three cultivars was similar to weight criterion (1.8 - 2.0 kg/fruit) of melon. In melon of non-net type ‘Aris’ there were no differences in fruit characteristics between nutrient solution supply methods, but total soluble solid (TSS) was higher in drip and capillary systems showing $15.6^{\circ}$Brix and 15.5$^{\circ}$Brix, respectively, than that of mist spray system showing $14.4^{\circ}$Brix. In melon of net type, ‘Earl's elite’, drip and capillary systems showed better fruit characteristics and higher values of TSS compared to mist spray system. The change in pH in the medium before and after melon cropping was not significant between systems, but the pH of upper layer within medium in all system was higher than that of lower layer. The mineral content within lower layer showed higher value than that of the upper layer in drip system, but reversed in capillary system.

• Korean Journal of Medicinal Crop Science
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• v.19 no.1
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• pp.16-23
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• 2011

This study was conducted to investigate the anti-inflammatory effects of Pruns mume, Schisandra chinensis, Chaenomeles sinensis-- Prunus mume mixtrue (PM) treatment on colitis induced in mice by dextran sodium sulfate (DSS) treatment. A total of 25 male BALB/c mice (average weight $20.7\;{\pm}\; 1.6 \;g$) were divided into 5 treatment groups and fed a commercial diet (A), PM administration (B), commercial diet + induced colitis by DSS (C), PM administration + induced colitis by DSS (D) and sulfasalazine + induced colitis by DSS (E). We found that PM treatment (D) and sulfasalazine (E) decreased the expression of $TNF-{\alpha}$ and COX-2 compared to the DSS-induced colitis group (C). The expression of IL-4, STAT6, $IFN-{\gamma}$, STAT1 was decreased in group D and group E compared to the colitis group (C), COX-2 and STAT1 were more decreased in group D. The serum IgE levels decreased in the PM treatment groups (C and D) compared to the non-PM treatment groups (A and B) although there was no significant difference between the PM treatment groups. It is notable that a therapeutic application of the PM extracts ameliorated DSS-induced colitis in mice.