• 한국환경보건학회지
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• 제36권1호
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• pp.44-51
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• 2010

This study aimed to develop analytical method for 8-isoprostanes as biomarkers for oxidative stress with LC/MS/MS technique and to apply the method for human urine samples. Analyzed compounds for urinary oxidative stress markers were 7 stereo-isomers of prostaglandins and the internal standard (iso-$PGF_{2{\alpha}}-d_4$) was used to adjust the recovery rate. The method for determining urinary iso-$PGF_{2{\alpha}}$ consisted of solid phase extraction and LC/MS/MS detection. Separation of isomers of prostaglandins completed by porous graphitic carbon column and buffer solution. Detection limits for urinary markers of oxidative stress, iso-$PGF_{2{\alpha}}$ with LC/MS/MS were 0.01 ng/ml by S/N ratio 3 and 0.028 ng/ml by calculated as to FDA method. The recovery (92.8~101.9%) and precision (8.8~20.7%) of analysis were feasible for detecting iso-$PGF_{2{\alpha}}$ in real human urine samples. We detected 4 isomers of prostaglandins in human urine samples. Mean (standard deviation) of urinary iso-$PGF_{2{\alpha}}$ concentration were 0.231 (0.117) ng/mg creatinine for smoking group and 0.154 (0.082) ng/mg creatinine for non-smoking group.

• 한국전자통신학회논문지
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• 제9권10호
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• pp.1071-1078
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• 2014

최근 빅데이터(Big Data)를 이용한 정보 제공 서비스가 확대되고 빅데이터 처리 기술 역시 IT 업체의 중요한 이슈로 학문적인 연구가 활발히 진행되고 있는 실정이다. 이에 본 논문에서는 R 프로그래밍을 기반으로 용접의 빅데이터 분석 및 추출을 통하여 용접사의 숙련된 패턴을 분석하고 분석된 결과를 비 숙련공에게 제공함으로써 용접 품질 및 용접 시간 단축 등의 용접 작업에 적용되는 비용을 절감하고자 한다. 용접은 숙련공이 되기 위하여 오랜 시간을 투자해야 하는 문제점이 있다. 이러한 단점을 해결하고자 숙련공들의 용접 패턴 분석을 위하여 다량의 패턴 변수에 R의 연관 규칙 알고리즘과 회귀분석 방식을 적용한다. 상위 N개의 규칙을 분석한 후 분석된 규칙의 변수에 따른 숙련자의 패턴을 분석한다. 본 논문에서는 분석된 용접 패턴 분석을 통해 실험 결과를 분석하여 전력소비량과 와이어 소모 길이에 대한 패턴 구조를 확인하였다.

• 한국측량학회지
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• 제26권2호
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• pp.117-127
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• 2008

우리나라는 여름철 집중호우와 태풍으로 인해 산사태가 빈번히 발생하고 있으며, 이로 인해 많은 인명피해와 경제적 손실이 발생하고 있다. 특히 강원도 지역의 대부분 도로가 산악지형에 위치하고 있어 산사태의 위험에 노출되어 있다. 따라서 산사태를 막기 위한 대책마련이 시급한 실정이며, 이를 위해 다양한 사면조사 및 사면유지관리를 위한 첨단 관측기술이 요구되고 있다. 최근에는 광섬유 센서, GPS, CCD 카메라, Total Station, 위성영상을 이용한 사면관측 기술이 활용되고 있으나 경제성, 정밀성, 효율성 등의 제약으로 인해 활용빈도가 떨어지고 있는 실정이다. 그러므로 본 연구에서는 지상라이다를 이용하여 시범사면을 모니터링한 후 정확도를 평가함으로써, 지상라이다의 사면관측을 위한 적용 가능성을 분석하고자 하였다. 그 결과 수 mm의 미세변위를 높은 정확도로 관측할 수 있었으며, 신속하게 사면의 지형정보를 획득할 수 있었다.

• 생약학회지
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• 제41권2호
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• pp.115-121
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• 2010

The present study was designed to explore an immunostimulating activity of crude extracts of Macrolepiota procera, and a combination therapy of cisplatin and Macrolepiota procera extracts which can potentiate the anti-cancer activity of cisplatin. For these, water extraction of Macrolepiota procera were performed at $4^{\circ}C$(MPE-4) and $100^{\circ}C$(MPE-100). In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous administration of MPE ($80-2,000{\mu}g$/mouse) inhibited tumor metastasis compared with tumor control. Peritoneal macrophages stimulated with MPE produced IL-12 as well as induced tumoricidal activity. In an analysis of NK-cell activity, i.v. administration of MPE ($200{\mu}g$/mouse) significantly augmented NK cytotoxicity to YAC-1 tumor cells. The combination treatments of cisplatin ($20{\mu}g$) and MPE ($100{\mu}g$) exhibited prolongation of lifespan in colon26-M3.1 tumor bearing mouse. These results suggested that MPE stimulate immune system non-specifically and application as adjuvant in cancer treatment.

• 대한원격탐사학회지
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• 제33권5_1호
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• pp.547-557
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• 2017

본 연구에서는 탐지하고자 하는 표적신호를 초기 엔드멤버로 하여 Iterative Error Analysis를 통해 배경물질들의 반사 스펙트럼을 순차적으로 엔드멤버로 추출하고, 추출된 엔드멤버들을 이용하여 분광 혼합분석함으로써 표적물질의 분포를 탐지하는 새로운 초분광 표적탐지 기법을 제안한다. 제안된 기법에서는 표적물질에 대한 점유율의 변화가 주어진 문턱값보다 작아질 때 엔드멤버 추출을 위한 반복을 멈추게 된다. 이 기법은 Orthogonal Subspace Projection과 같은 모델 기반 표적 탐지기법들과 달리 사전에 엔드멤버들을 확보해야 할 필요가 없으며, Matched Filter와 같은 확률론적 표적 탐지 기법들과 달리 배경 전체를 하나의 신호로 특징화하지 않기 때문에 표적의 희소성 여부에 의한 영향을 받지 않는다는 장점을 가지고 있다. 실제 항공 초분광 영상자료 및 다양한 인공 표적물질들이 삽입된 모의 초분광 영상자료를 이용한 실험 결과, 제안된 방법이 희소 및 비 희소 표적의 탐지에 매우 효과적임이 확인되었다. 제안된 방법은 표적 물체 탐지뿐만 아니라 광물, 오염물질 등 자원 및 환경 분야에서 다양한 피복 물질을 탐지하는데 효과적으로 사용될 수 있을 것으로 기대된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제18권7호
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• pp.41-53
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• 2013

The study is to propose the optimal separation technique of heavy metals (Pb and Zn) contaminated in soil for improving the removal efficiency by various applicable techniques. The heavy metal contaminated soil samples near abandoned mine X-1 and X-2 were used for the study. Firstly, the wet classification process was shown more than 80% of removal efficiency for lead and zinc. Meanwhile, the magnetic separation process was shown low removal efficiency for lead and zincs because those heavy metals were non-magnetic materials. For the next step, the flotation separation process was shown approximately 24.4% of removal efficiency for zinc, while the gravity concentration process was shown approximately 57% of removal efficiency for lead, and 19.9% of removal efficiency for zinc, respectively. Therefore, zinc contaminated in soil would be effectively treated by the combination technique of the wet classification and the flotation technique. Meanwhile, lead contaminated in soil would be effectively treated by the combination technique of the wet classification process and the flotation process. Furthermore, the extraction of organic matter was shown more effective with aeration, 3% of hydrogen peroxide and 3% of lime such as calcium hydroxide.

• 한국약용작물학회지
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• 제26권1호
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• pp.26-31
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• 2018

Background: Angelica gigas, A. sinensis, and A. acutiloba are commercially important in the herbal medicine market, and among them, A. gigas has the highest economic value and price. However, their similar morphological traits are often used for fraud. Despite their importance in herbal medicine, recognition of the differences between Angelica species is currently inadequate. Methods and Results: A multiplex polymerase chain reaction (PCR) method was developed for direct detection and identification of A. gigas, A. sinensis, and A. acutiloba. The gene for the distinction of species was targeted at ITS in the nucleus and trnC-petN gene in chloroplasts. The optimized multiplex PCR in the present study utilized each Angelica species-specific primer pairs. Each primer pair yielded products of 229 base pairs (bp) for A. gigas, 53 bp for A. sinensis, 170 bp for A. acutiloba. Additionally non-specific PCR products were not detected in similar species by species-specific primers. Conclusions: In the present study, a multiplex-PCR assay, successfully assessed the authenticity of Angelica species (A. gigas, A. sinensis, and A. acutiloba). and whole genome amplification (WGA) was performed after DNA extraction to identify, the species in the product. The detection method of raw materials developed in the present study could be applied to herbal medicine and health functional food management.

• Journal of Microbiology
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• 제40권4호
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• pp.274-281
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• 2002

The competitive quantitative PCR method targeting pcbC gene was developed for monitoring 4-chlorobiphenyl(4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The method involves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the elec-trophoresed PCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strain DJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with 4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCR in comparison with 4CB degradation and the result was compared to those obtained by using the conventional cultivation method. We successfully detected and monitored 4CB-degrading bacteria in each microcosm and found a significant linear relationship between the number of 4CB-degrading bacteria and the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experiments suggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CB- degrading bacteria appears to be rapid, sensitive and more suitable than the microbiological approach in estimating the capacity of 4CB biodegradation in environmental samples.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1528-1528
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• 2001

Ginseng cultivated in different country or growing condition has generally different components such as saponin and protein, and it relates to efficacy and action. Protein content assumes by nitrogen content in ginseng radix. Nitrogen content could be determined by chemical analysis such as kjeldahl or extraction methods. However, these methods require long analysis time and result environmental pollution and sample damage. In this work we investigated possibility of non-destructive determination of nitrogen content in ginseng radix using near-infrared spectroscopy. Ginseng radix, root of Panax ginseng C. A. Meyer, was studied. Total 120 samples were used in this study and it was consisted of 6 sample sets, 4, 5 and 6-year-old Korea ginseng and 7, 8 and 9-year-old China ginseng, respectively. Each sample set has 20 sample. Nigrogen content was measured by electronic analysis. NIR reflectance spectra were collected over the 1100 to 2500 nm spectral region with a InfraAlyzer 500C (Bran+Luebbe, Germany) equipped with a halogen lapmp and PbS detector and data were collected every 2 nm data point intervals. The calibration models were carried out by multiple linear regression (MLR) and partial least squares (PLS) analysis using IDAS and SESAME software. Result of electronic analysis, Korean ginseng were different mean value in nitrogen content of China ginseng. Ginseng tend to generally decrease the nitrogen content according as cultivation year is over 6 years. The MLR calibration model with 8 wavelengths using IDAS software accurately predicted nitrogen contents with correlation coefficient (R) and standard error of prediction of 0.985 and 0.855%, respectively. In case of SESAME software, the MLR calibration with 9 wavelength was selected the best calibration, R and SEP were 0.972 and 0.596%, respectively. The PLSR calibration model result in 0.969 of R and 0.630 of RMSEP. This study shows the NIR spectroscopy could be applied to determine the nitrogen content in ginseng radix with high accuracy.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권6호
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• pp.488-496
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• 2004

Oral & Maxillofacial surgery can lead to complications that result in abnormal sensation or movement. Inferior alveolar nerve(IAN) injury can result in dysesthesia, paresthsia of the lower lip and chin, so patients presenting with IAN damage suffer from sensory loss. But diagnosis of the nerve injury is largely limited to the subjective statements made by the patient. Distribution of sympathetic nerves parallels the distribution of the somatosensory nerves. Loss of sensory tone causes a concomitant loss of sympathetic activity, resulting in vasodilation of the cutaneous blood vessels that demonstrates greater heat loss. Digital infrared thermographic imaging(DITI) detects infra-red radiation given off by body. DITI can detect minute difference in temperature from different parts of the body and translates the amount of heat into quantitative data. The area of different temperature correlated with pain or disease can be visualized by corresponding color. The objective of this study was to determine the efficacy of DITI in objectively assessing IAN injury. The 19 normal subjects and the 14 patients underwent DITI scan. The normal subjects received unilateral IAN block anesthesia with 2 ml of 2% lidocaine (IAN bolck group) to evaluate temporary alteration in nerve function. Patient group were patients with unilateral IAN damage (dysesthesia or paresthesia) after surgical treatment(Mn. 3rd molar Extraction, etc.). The surgical procedure performed within 6 months of test. The results were as follows. 1. No significant differences in temperature were found between left and right sides of the lower lip and chin in the control group. 2. Significant temperature differences were found between the anesthetized and non-anesthetized sides of the lower lip and chin in the IAN block group. 3. Significant temperature differences were found between the involved and uninvolved sides of the lower lip and chin areas of the experimental group. The results of the study show that DITI can be an useful and effective means of objectively assessing and visualizing IAN damage.