• Title/Summary/Keyword: Non-extraction

Search Result 1,067, Processing Time 0.029 seconds

Static Supercritical Fluid Extraction of PCBs from Soil Matrix (정적 초임계유체 방식에 의한 토양 중의 PCBs 추출)

  • Ryoo, Keon-Sang;Lee, Won-Kyoung;Hong, Yong-Pyo;Oh, In-Gyung;Kim, Yong-Gyun
    • Journal of the Korean Chemical Society
    • /
    • v.47 no.6
    • /
    • pp.578-584
    • /
    • 2003
  • Polychlorinated biphenyls (PCBs) known as environmental contaminants in soil were analyzed by the soil pollution standard process test and the static supercritical $CO_2$ extraction mode. It was shown that the percent average recoveries of PCBs by the soil pollution standard process test were ranged in 25-35% and the corresponding standard deviations were above 10%. In contrast, the percent average recoveries of PCBs by the static supercritical $CO_2$ extraction mode were 2-2.5 times higher and standard deviations were within 7%. These results indicate that static supercritical $CO_2$ extraction mode may be a useful alternative to sample pretreatment certified by the soil pollution standard process test. The increasing supercritical $CO_2$ pressure from 1130 psi to 1996 psi at $40^{\circ}C$ enhanced the recovery of all PCB congeners from soil. However, at same Tc and Pc, the equilibrium time (5 versus 60 minutes) had no effect on the recovery of each PCB congener. Finally, similar PCB recoveries were obtained under the same extraction condition, regardless of the molecular weight and structure (coplanar versus non-coplanar) of PCB congeners.

Extraction Characteristics of Saponin and Acidic Polysaccharide Based on the Red Ginseng Particle Size (홍삼의 입자크기에 따른 사포닌 및 산성다당체의 추출 특성)

  • Cho, Chang-Won;Kim, Sang-Wook;Rho, Jeong-Hae;Rhee, Young-Kyung;Kim, Kyung-Tack
    • Journal of Ginseng Research
    • /
    • v.32 no.3
    • /
    • pp.179-186
    • /
    • 2008
  • Effect of pulverization on total solid, crude saponin, and acidic polysaccharide contents of dried red ginseng main root were tested. Several particle size samples, including red ginseng main root (non pulverized), $10{\sim}40$ mesh powder, $40{\sim}100$ mesh powder, and >100 mesh powder were used in the extraction. The sequential solvent extraction method (1st: 70% EtOH at $70^{\circ}C$ for 12 hr, 2nd: 70% EtOH at $70^{\circ}C$ for 12 hr, 3rd: water at $70^{\circ}C$ for 12 hr) was applied to extract the saponins and acidic polysaccharide. Extraction yield of total solid of pulverized red ginseng ($10{\sim}40$ mesh size) was increased to 20% compared with that of non-pulverized. Especially, the crude saponin content of pulverized red ginseng ($10{\sim}40$ mesh size) showed an increase of 47% over non-pulverized. No difference in the component ratio was observed by pulverization, when the individual ginsenosides were quantified by HPLC. Also, extraction yield of acidic polysaccharide of pulverized red ginseng ($10{\sim}40$ mesh size) was increased 57% compared with that of non-pulverized. The results suggested that pulverization might be useful for increasing the extraction yield of red ginseng components.

pH-Dependence of RNA Extraction for Norovirus by TRIzol Method (TRIzol을 이용한 노로바이러스 RNA 추출의 pH 의존성)

  • Jhon, Deok-Young
    • Journal of Food Hygiene and Safety
    • /
    • v.33 no.1
    • /
    • pp.71-76
    • /
    • 2018
  • Norovirus is a leading cause of sporadic pathogenic non-bacterial gastroenteritis worldwide. For the detection of norovirus, reverse transcription real-time PCR (RT qPCR) has quickly become a major tool due to its sensitivity and specificity. However, accurate viral RNA extraction methods are essential for RT qPCR analysis. TRIzol reagents are used to extract RNA from biological materials and are therefore widely used for norovirus RNA extraction. In this study, the yield of viral RNA extraction using TRIzol from genogroup II (GII) among the human norovirus genogroup I (GI) and GII, and murine norovirus (GV) depended on the pH of the virus sample solution. The yield of RNA extraction was higher at the alkaline pH than in the acidic region compared with the Ct (threshold cycle) value of the real-time PCR. From the results of this study, it was found that the pH condition is very important for the quantitative analysis of norovirus by extracting GII RNA using TRIzol.

A Comparison of Global Feature Extraction Technologies and Their Performance for Image Identification (영상 식별을 위한 전역 특징 추출 기술과 그 성능 비교)

  • Yang, Won-Keun;Cho, A-Young;Jeong, Dong-Seok
    • Journal of Korea Multimedia Society
    • /
    • v.14 no.1
    • /
    • pp.1-14
    • /
    • 2011
  • While the circulation of images become active, various requirements to manage increasing database are raised. The content-based technology is one of methods to satisfy these requirements. The image is represented by feature vectors extracted by various methods in the content-based technology. The global feature method insures fast matching speed because the feature vector extracted by the global feature method is formed into a standard shape. The global feature extraction methods are classified into two categories, the spatial feature extraction and statistical feature extraction. And each group is divided by what kind of information is used, color feature or gray scale feature. In this paper, we introduce various global feature extraction technologies and compare their performance by accuracy, recall-precision graph, ANMRR, feature vector size and matching time. According to the experiments, the spatial features show good performance in non-geometrical modifications, and the extraction technologies that use color and histogram feature show the best performance.

Upper and lower second premolar extraction treatment case - Treatment strategy for Class III borderline cases (상하악 제2소구치 발거 치료 증례 - III급 부정교합 경계증례의 치료전략)

  • Kim, Tae-Kyung;Kim, Jong-Tae;Yang, Won-Sik
    • The korean journal of orthodontics
    • /
    • v.32 no.3 s.92
    • /
    • pp.185-194
    • /
    • 2002
  • When treating borderline cases which have mild crowding, non-extraction treatment may be considered firstly. But crowding may be reappeared by relapse and it may have problems in esthetics and stability. Secondarily four first premolar extraction treatment may be considered. But this may cause dish-in face by overretracting anterior teeth. In this cases, extraction of four second premolar is preferred because this resolves crowding without aggravating profile and has good stability after treatment. So we review cases treated by four second premolar extraction which show good treatment results and stability. The patients had good profile, Class I molar relationship, mild crowding and skeletal discrepancy and their growth had almost completed.

Line Segments Extraction by using Chain Code Tracking of Edge Map from Aerial Images (항공영상으로부터 에지 맵의 체인코드 추적에 의한 선소추출)

  • Lee Kyu-won;Woo Dong-min
    • Journal of the Korean Institute of Intelligent Systems
    • /
    • v.15 no.6
    • /
    • pp.709-713
    • /
    • 2005
  • A new algorithm is proposed for the extraction of line segments to construct 3D wire-frame models of building from the high-resolution aerial images. The purpose of this study Is the accurate and effective extraction of line segments, considering the problems such as discordance of lines and blurred edges existing in the conventional methods. Using the edge map extracted from aerial images, chain code tracking of edges was performed. Then, we extract the line segments considering the strength of edges and the direction of them. SUSAN (Smallest Uni-value Segment Assimilating Nucleus) algorithm proposed by Smith was used to extract an edge map. The proposed algorithm consists of 4 steps: removal of the horizontal, vertical and diagonal components of edges to reduce non-candidate point of line segments based on the chain code tracking of the edge map, removal of contiguous points, removal of the same angle points, and the extraction of the start and end points to be line segments. By comparing the proposed algorithm with Boldt algorithm, better results were obtained regarding the extraction of the representative line segments of buildings, having relatively less extraction of unnecessary line segments.

Enzyme-assisted extraction of Ecklonia cava fermented with Lactobacillus brevis and isolation of an anti-inflammatory polysaccharide

  • Lee, Won-Woo;Ahn, Ginnae;Wijesinghe, W.A.J.P.;Yang, Xiudong;Ko, Chang-Ik;Kang, Min-Cheol;Lee, Bae-Jin;Jeon, You-Jin
    • ALGAE
    • /
    • v.26 no.4
    • /
    • pp.343-350
    • /
    • 2011
  • Fermentation and enzyme-assisted extraction (EAE) improve nutritional and functional properties of foods by increasing the extraction of active compounds, ingestion rates, and body absorption. In this study, we investigated whether applying the EAE process improves the extraction and isolation efficiency of a polysaccharide from fermented Ecklonia cava (FE), which inhibited NO production in lipopolysaccharide (LPS)-activated RAW 264.7 cells. The results showed that the FE using the fungi Candida utilis and two different bacteria, namely Lactobacillus brevis and Saccharomyces cerevisiae increased protein and carbohydrate contents in comparison with those in non-fermented E. cava (NE). Aqueous extracts of fermented E. cava increased extraction yields and carbohydrate content, compared with the aqueous extract of NE. In addition, treating LPS-stimulated RAW 264.7 cells with aqueous extracts resulted in reduced NO production compared to that in LPS-treated cells. Ten EAEs of L. brevis-fermented E. cava (LFE) improved NO inhibitory effects in LPS-activated RAW 264.7 cells and the Viscozyme extract (VLFE) from the resulting extracts showed the highest NO inhibitory effect. We found that the >30 kDa fraction of VLFE led to markedly high inhibition of LPS-induced NO production as compared to that in the <30 kDa fraction. The crude polysaccharide isolated from >30 kDa fraction (VLFEP) consisted of fucose and markedly decreased NO production induced by LPS stimulation. VLFEP could be useful as an anti-inflammatory agent to suppress macrophage activation.

Effect of Heat Treatment, Ethanol Content, Extraction Time and Ratio of Solvent on the Efficiency of Polyphenol Extraction from Licorice Root (Glycyrrhizauralensis) (감초폴리페놀 추출효율에 있어 열처리, 에탄올 농도, 추출시간 및 용매비율이 미치는 영향 탐색)

  • Chae, Jung-Il;Ryu, Kyeong-Seon;Seo, Kang-Seok;Kim, Kyung-Hoon;Oh, Young-Kyoon;Jang, Sun-Sik;Choi, Chang-Weon;Choi, Nag-Jin
    • Korean Journal of Organic Agriculture
    • /
    • v.20 no.3
    • /
    • pp.399-409
    • /
    • 2012
  • Effects of pretreatment and extraction conditions on total polyphenol yield from licorice root were investigated using statistical method. For pretreatment, heat treatment at $121^{\circ}C$ for 10 min was applied. Licorice root content in solvent (10, 20, and 30%) ethanol concentration (20, 40, and 60%) and reaction time (1, 2, and 3 h) were used as variables for extraction conditions. Two experiments, with heat treated and no treated licorice, were prepared with same experimental design. Box behnken design was employed and produced a total of 15 trials. Total polyphenol yield from licorice root was not affected by heat treatment. Among variables, licorice content in solvent showed most significant effect regardless of other variables (p<0.05). Finally, optimum conditions for the extraction of total polyphenol from licorice root was detected as following: 10% of licorice in solvent, 52% ethanol as solvent, 2 h of reaction time and non-heat treatment and the extraction yield from optimized condition was 17.6 mg/g licorice root.

Extraction Characteristics of $Cr^{6+}$ from Aqueous Solutions with PC-88A, Alamine 336 and Aliquat 336 (PC-88A, Alamine 336 및 Aliquat 336에 의한 수용액중$Cr^{6+}$의 추출특성)

  • Kim Sung Gyu;Lee Hwa Young;Oh Jong Kee
    • Resources Recycling
    • /
    • v.11 no.6
    • /
    • pp.18-23
    • /
    • 2002
  • A study on the solvent extraction of hexavalent chromium from aqueous solutions has been investigated. The results showed that the extraction efficiency of chromium increased in proportion to extractant concentration. Aliquat 336 of quarternary amine effectively extracted hexavalent chromium and was superior to Alamine 336. And the stabilizers like polyhydric alcohols of octanol, decanol and dodecanol showed the similar effect fur prevention of third phase and a little promotion of extraction efficiency in comparison with non-use of polyhydric alcohols. On the other hand, in solvent extraction of hexavalent chromium from aqueous solutions, the hexavalent chromium was completely extracted at pH range lower than 7 with 1% Aliquat 336 as extractant and 5% decanol as stabilizer and the hexavalent chromium in extractant was completely stripped with 1M sodium hydroxide solution in the stripping step.

Analysis of Korean Patent Information for Air Extraction Cupping Devices

  • Lee, Tae Wook;Kim, Ku Weon;Kim, Hyo Jin;Choi, Ji Won;Choi, Seo Young;Lee, Byung Ryul;Yang, Gi Young
    • Journal of Acupuncture Research
    • /
    • v.36 no.1
    • /
    • pp.28-32
    • /
    • 2019
  • Background: The purpose of this study was to collate inventors' improvements of air extraction cupping technology by reviewing patent applications in Korea. The cost of procurement and use of air extraction cupping devices are covered by health insurance. Methods: Patents registered in Korea for air extraction cupping devices from August 1992 to January 2018 (registration, expiration, rejection, abandonment, and disclosure) were analyzed to determine technology trends. The Korean patent search engine used was Kipris (www.kipris.or.kr). Results: Sixty-seven Korean patents for air extraction cupping devices were retrieved. Most patents focused on design of the top valve of the device, the device at the bottom, an internal device, and the disposable cupping glass. In total 17.9% of patents were registered and 64.2% were abolished [either from non-payment of registration fees (93%) or expiration of the patent after 10 years (7%)]. The average registration period was 3.7 years. Conclusion: The patents retrieved for this study focused on the development of the air exhaust valve, disposable cups, and skin adhesion maintenance technology in cupping devices that use air extraction. The average registration period was 3.7 years, meaning the patents that were not commercialized, expired without additional registration renewals. It is important to acquire a New Excellent Technology certificate for a cupping device to promote commercialization.