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Effect of Heat Treatment, Ethanol Content, Extraction Time and Ratio of Solvent on the Efficiency of Polyphenol Extraction from Licorice Root (Glycyrrhizauralensis)  

Chae, Jung-Il (Department of Dental Pharmacology, School of Dentistry, Chonbuk National University)
Ryu, Kyeong-Seon (Department of Animal Science, Chonbuk National University)
Seo, Kang-Seok (Department of Animal Science & Technology, Sunchon National University)
Kim, Kyung-Hoon (Animal Nutrition and Physiology Division, National Institute of Animal Science, RDA)
Oh, Young-Kyoon (Animal Nutrition and Physiology Division, National Institute of Animal Science, RDA)
Jang, Sun-Sik (Hanwoo experiment, National Institute of Animal Science, RDA)
Choi, Chang-Weon (Department of Animal Resources, Daegu University)
Choi, Nag-Jin (The Institute of Rare Earth for Biological Application, Chonbuk National University)
Publication Information
Korean Journal of Organic Agriculture / v.20, no.3, 2012 , pp. 399-409 More about this Journal
Abstract
Effects of pretreatment and extraction conditions on total polyphenol yield from licorice root were investigated using statistical method. For pretreatment, heat treatment at $121^{\circ}C$ for 10 min was applied. Licorice root content in solvent (10, 20, and 30%) ethanol concentration (20, 40, and 60%) and reaction time (1, 2, and 3 h) were used as variables for extraction conditions. Two experiments, with heat treated and no treated licorice, were prepared with same experimental design. Box behnken design was employed and produced a total of 15 trials. Total polyphenol yield from licorice root was not affected by heat treatment. Among variables, licorice content in solvent showed most significant effect regardless of other variables (p<0.05). Finally, optimum conditions for the extraction of total polyphenol from licorice root was detected as following: 10% of licorice in solvent, 52% ethanol as solvent, 2 h of reaction time and non-heat treatment and the extraction yield from optimized condition was 17.6 mg/g licorice root.
Keywords
licorice root; total polyphenol; aqueous ethanol; box behnken design; optimization;
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