• Title/Summary/Keyword: Non-causal

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Detection of the Causal Agent of Bacterial Wilt, Ralstonia solanacearum in the Seeds of Solanaceae by PCR (가지과 종자에서 Ralstonia solanacearum의 검출을 위한 PCR 방법)

  • Cho, Jung-Hee;Yim, Kyu-Ock;Lee, Hyok-In;Baeg, Ji-Hyun;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.184-190
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    • 2011
  • Ralstonia solanacearum, a causal agent of bacterium wilt is very difficult to control once the disease becomes endemic. Thus, Ralstonia solanacearum is a plant quarantine bacterium in many countries including Korea. In this study, we developed PCR assays, which can detect Ralstonia solanacearum from the Solanaceae seeds. Primers RS-JH-F and RS-JH-R amplified specifically a 401 bp fragment only from Ralstonia solanacearum race 1 and race 3. The nested PCR primers, RS-JH-F-ne and RS-JH-R-ne that were designed inside of 1st PCR amplicon amplified specifically a 131 bp fragment only from Ralstonia solanacearum race 1 and race 3. The primers did not amplify any non-specific DNA from the seed extracts of the Solanaceae including tomato and pepper. When detection sensitivity were compared using the Solanaceae seeds inoculated with target bacteria artificially, the nested PCR method developed in this study 100 times more sensitive than ELISA and selective medium. Therefore, we believe that the PCR assays developed in this work is very useful to detect Ralstonia solanacearum in the Solanaceae seeds.

Transfer of Arsenic and Heavy Metals Existed as Acid Extractable and Reducible Formsfrom Flooded Soilsto Rice Plant (담수토양 내 비소 및 중금속의 존재형태(산추출형, 환원형)에 따른 식물체(벼) 전이특성)

  • Koh, Il-Ha;Kim, Jung-Eun;Ji, Won-Hyun
    • Journal of Environmental Impact Assessment
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    • v.31 no.5
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    • pp.296-309
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    • 2022
  • This study investigated the mobility of inorganic elements (As, Cd, Pb, and Zn) that existed as acid extractable and reducible forms in flooded soils with a pot experiment involving rice cultivation. In general, it is known that soil inorganic elements that existed as an acid extractable form which includes exchangeable, carbonates, non-specifically sorbed, and specifically sorbed have mobility. However, the result of the experiment revealed that each inorganic elements of rice roots grown from flooded soils had different characteristics. The concentrations of Arsenic existed as both forms and the concentrations of cadmium and lead existed as a reducible form in the soils showed a high causal relationship with the concentrations of those elements in the roots of rice plants. The concentrations of zinc, an essential plant element, didn't show a causal relationship. Therefore it is necessary to consider the soil's environmental characteristics such as drained/flooded condition, oxidation/reduction condition, etc. for the mobility assessment of inorganic elements. The concentrations of the reducible form of arsenic, cadmium, and lead in flooded environment such as a paddy field should be also considered because the mobility of these elements combined with Fe/Mn increases in the reduction condition.

A study on the user satisfaction evaluation model of the smart learning system - Focusing on www.basic-edu.net usability evaluation results - (스마트러닝 시스템의 이용만족도 평가모형 연구 - www.basic-edu.net 사용성 평가 결과를 중심으로 -)

  • Park In-chan;Huh Hyeong-sun;Jeon Gwan-cheol;Ahn Jin-ho
    • Journal of Service Research and Studies
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    • v.11 no.4
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    • pp.67-76
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    • 2021
  • The importance of smart learning is increasing as the speed of development of non-face-to-face services increases due to the influence of COVID-19. This study is the user satisfaction evaluation model that utilizes the causal relationship between variables used for evaluation, focusing on the usability evaluation results of the learning disability intervention service (www.basic-edu.net) according to the need to evaluate the use satisfaction of the smart learning system. To this end, theoretical studies were conducted on smart learning and learning disability intervention services, www.basic-edu.net, usability evaluation of learning disability intervention systems, and use satisfaction evaluation models. And based on the results, a hypothesis was presented on the user satisfaction evaluation model of the smart learning system. The experimental method allowed 40 students and parents across the country to use the www.basic-edu.net service and was evaluated for its usability. In addition, using this data, the hypothesis was verified using regression analysis based on four variables: ease of use, interest, self-learning, and satisfaction with use. As a result of the hypothesis verification, it was found that the causal relationship of all hypotheses from H1 to H4 was significant.

Risk assessment for development of consecutive shield TBM technology (연속굴착형 쉴드 TBM 기술 개발을 위한 리스크 평가)

  • Kibeom Kwon;Hangseok Choi;Chaemin Hwang;Sangyeong Park;Byeonghyun Hwang
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.26 no.4
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    • pp.303-314
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    • 2024
  • Recently, the consecutive shield tunnel boring machine (TBM) has gained attention for its potential to enhance TBM penetration rates. However, its development requires a thorough risk assessment due to the unconventional nature of its equipment and hydraulic systems, coupled with the absence of design or construction precedents. This study investigated the causal relationships between four accidents and eight relevant sources associated with the consecutive shield TBM. Subsequently, risk levels were determined based on expert surveys and a risk matrix technique. The findings highlighted significant impacts associated with collapses or surface settlements and the likelihood of causal combinations leading to misalignment. Specifically, this study emphasized the importance of proactive mitigation measures to address collapses or surface settlements caused by inadequate continuous tail void backfill or damaged thrust jacks. Furthermore, it is recommended to develop advanced non-destructive testing technology capable of comprehensive range detection across helical segments, to design a sequential thrust jack propulsion system, and to determine an optimal pedestal angle.

The Association of Lead Biomarkers of Lead Workers with Airborne Lead Concentration in Lead Industries (납 사업장의 공기 중 납 농도 및 납 노출 근로자들의 납 관련 생물학적 노출 지표의 관련성에 관한 조사)

  • Kim, Nam-Soo;Kim, Jin-Ho;Jang, Bong-Ki;Kim, Hwa-Sung;Ahn, Kyu-Dong;Lee, Byung-Kook
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.17 no.1
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    • pp.43-52
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    • 2007
  • This study was designed to investigate the difference of airborne lead concentration by type of lead industries and type of lead exposure and to evaluate their association with lead biomarkers of lead workers in 11 lead using industries. Total of 182 lead workers (male: 167, female: 15) from 11 lead industries were participated for this study from March, 2004 to August, 2005. Airborne lead concentration were measured by representative personal sampling of workers in each unit workplace and applied same concentration value to the workers in the same unit workplace who did not measure their airborne lead with personal air sampling. Tibia lead, blood lead, zinc protoporphyrin in whole blood, ${\delta}$-aminolevulinic acid in urine, hemoglobin and hematocrit were selected as study variables of indices of lead exposure. Information about type of lead exposure (fume or non-fume other), age, work duration, smoking & drinking habit were also collected. Significant differences were seen in the means of zinc protoporphyrin, blood lead and tibia lead in lead workers by different airborne lead concentration in workplace. While blood lead and tibia lead in lead workers were significantly higher in secondary smelting than other types of lead industries, zinc protoporphyrin, ${\delta}$-aminolevulinic acid in urine and airborne lead concentration were significantly higher in litharge manufacturing. While the mean blood lead was significantly higher in the lead workers working in fume type unit workplace than those of non-fume lead workers, the mean airborne lead concentration of fume workers was significantly lower than non-fume lead workers. In the multiple regression analysis of airborne lead concentration and the type of lead exposure on tibia lead and lead exposure indices after adjustment of related covariates, airborne lead concentration was statistically significantly associated with blood lead and tibia lead, but the type of lead exposure was only associated with blood lead. To verify the causal association of airborne lead concentration on blood lead and tibia lead, further studies are needed.

A Study of Hazard Analysis and Monitoring Concepts of Autonomous Vehicles Based on V2V Communication System at Non-signalized Intersections (비신호 교차로 상황에서 V2V 기반 자율주행차의 위험성 분석 및 모니터링 컨셉 연구)

  • Baek, Yun-soek;Shin, Seong-geun;Ahn, Dae-ryong;Lee, Hyuck-kee;Moon, Byoung-joon;Kim, Sung-sub;Cho, Seong-woo
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.19 no.6
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    • pp.222-234
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    • 2020
  • Autonomous vehicles are equipped with a wide rage of sensors such as GPS, RADAR, LIDAR, camera, IMU, etc. and are driven by recognizing and judging various transportation systems at intersections in the city. The accident ratio of the intersection of the autonomous vehicles is 88% of all accidents due to the limitation of prediction and judgment of an area outside the sensing distance. Not only research on non-signalized intersection collision avoidance strategies through V2V and V2I is underway, but also research on safe intersection driving in failure situations is underway, but verification and fragments through simple intersection scenarios Only typical V2V failures are presented. In this paper, we analyzed the architecture of the V2V module, analyzed the causal factors for each V2V module, and defined the failure mode. We presented intersection scenarios for various road conditions and traffic volumes. we used the ISO-26262 Part3 Process and performed HARA (Hazard Analysis and Risk Assessment) to analyze the risk of autonomous vehicle based on the simulation. We presented ASIL, which is the result of risk analysis, proposed a monitoring concept for each component of the V2V module, and presented monitoring coverage.

Impact of Oil Price Shocks on Stock Prices by Industry (국제유가 충격이 산업별 주가에 미치는 영향)

  • Lee, Yun-Jung;Yoon, Seong-Min
    • Environmental and Resource Economics Review
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    • v.31 no.2
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    • pp.233-260
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    • 2022
  • In this paper, we analyzed how oil price fluctuations affect stock price by industry using the non-parametric quantile causality test method. We used weekly data of WTI spot price, KOSPI index, and 22 industrial stock indices from January 1998 to April 2021. The empirical results show that the effect of changes in oil prices on the KOSPI index was not significant, which can be attributed to mixed responses of diverse stock prices in several industries included in the KOSPI index. Looking at the stock price response to oil price by industry, the 9 of 18 industries, including Cloth, Paper, and Medicine show a causality with oil prices, while 9 industries, including Food, Chemical, and Non-metal do not show a causal relationship. Four industries including Medicine and Communication (0.45~0.85), Cloth (0.15~0.45), and Construction (0.5~0.6) show causality with oil prices more than three quantiles consecutively. However, the quantiles in which causality appeared were different for each industry. From the result, we find that the effects of oil price on the stock prices differ significantly by industry, and even in one industry, and the response to oil price changes is different depending on the market situation. This suggests that the government's macroeconomic policies, such as industrial and employment policies, should be performed in consideration of the differences in the effects of oil price fluctuations by industry and market conditions. It also shows that investors have to rebalance their portfolio by industry when oil prices fluctuate.

Impacts of Health Insurance Coverage Expansion on Health Care Utilization and Health Status (건강보험 보장성 확대가 의료이용 및 건강수준에 미치는 영향)

  • Bae, Ji-Young
    • Korean Journal of Social Welfare Studies
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    • v.41 no.2
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    • pp.35-65
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    • 2010
  • The purpose of this study is to identify the relationship between health insurance and health by evaluating the impacts of health insurance coverage expansion on health care utilization and health status. To analyze the causal relationship between health insurance and health, this study employed a "difference-in-difference method" that could compare changes in health care utilization and health status across groups in health insurance coverage expansion in 2005. The researcher predicted that the expansion of health insurance coverage would be an exogenous source of variation in the prices of health service use. First, the difference-in-differences estimator between 'illness group' and 'non-illness group' revealed that the increase in coverage of inpatient care services would result from the increases in the stay of length of 'non-illness group' rather than that of 'illness group'. However, the difference-in-differences estimator between 'serious illness group' and 'chronic illness group' identified that the policy change that focuses on expansion of the coverage for 'serious illness' effects on the increases in health care utilization and promotion of health status. In summary, the changes of health insurance coverage focusing on serious illness and inpatient care have positive effects on health care utilization and health status of serious illness group. But, 'non-illness groups' with acute illness receive more benefits from the policy change than 'illness group' with chronic illness.

A Study on Smartphone Addiction, Mental health and Impulsiveness For High School Students at Korea (고등학생의 스마트폰 중독과 정신건강, 충동성에 관한 연구)

  • Yu, Myeong-Ok;Ju, Se-Jin;Kim, Joo Hyun
    • Journal of Digital Convergence
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    • v.12 no.4
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    • pp.409-418
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    • 2014
  • The purpose of this study is to verify the causal relationship between the smartphone addiction proneness, mental health, and impulsiveness of high school students in Korea. 804 first and second year high school students participated in the survey that included the Smartphone addiction scale, Korean Brief Mental Diagnosis Exam - the highest 25% of total score (Addictive SmartPhone Use Group : ASPUG 213 persons), the lowest 25% of total score(Non-Addictive SmartPhone Use Group : NASPUG 204 persons), BIS-II Impulsiveness Scale and additional questions asking for demographical characteristics. The outcomes of this study were as follows. First, sex, grade of high school, academic accomplishment, student's satisfaction with school life, monthly allowance, the level of parental respect, having a opposite sex friend were related to smartphone addiction and impulsiveness. Second, ASPUG had worse mental health and impulsiveness than NASPUG, and the difference was statistically significant. Third, Students showed higher levels of mental health 9 subareas - anxiety, depression, phobic anxiety, Somatization, obsessive compulsive, interpersonal sensitivity, hostility, Paranoid ideation, Psychosis as their smartphone use increased.

Screening Assay for Identification of Endocrine Disruptors with Androgen Activities using LNCaP Cells (LNCaP 세포주를 이용한 내분비계장애물질중 안드로겐성 확인시험을 위한 검색법)

  • 김진호;정혜주;김영옥;정승태;박재현;조대현;김동섭
    • Toxicological Research
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    • v.18 no.1
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    • pp.59-64
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    • 2002
  • Substantial evidences have been accumulated about the hormone-like effects of exogenous substances such as pesticides and industrial chemicals during past years. The effects of these substances on the endocrine system are believed to be either enhancing or reducing of various endocrine action. It is necessary to identify putative causal agents by the batter system and to assess their ability to disrupt the endocrine system. A variety of in vitro and In vivo approaches have been used to determine the androgenic effects of environmental chemicals. To establish the method for assessment of the putative endocrine disruptors with androgenic activity, we carried out the cell proliferation assay by MTS method after treatment with the various concentration of testosterone in LNCaP cells (human prostatic cancer cell line) and also observed the expression of androgen-related genes by quantitative RT-PCR. In the cell proliferation assay, the results showed that the grouth of LNCaP cells increased within level of at least 10pM testosterone. We measured by quantitative RT-PCR method on the effects of testosterone on mRNA expression of androgen receptor (AR), prostate-specific antigen (PSA), bone morphogenetic protein (BMP) and BMP receptor (BMPR) In LNCaP cells. The results demonstrated that mRNA expression of PSA and BMPR-IB was observed differently within level of at least 0.01 pM testosterone compared with non-treated control. These observations suggest that the detection of PSA and BMPR-IB mRNA by the quantitative RT-PCR in LNCaP cells is very sensitive method to identify the endocrine disruptors to have the androgenic effects.