• Journal of Applied Biological Chemistry
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• 제54권2호
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• pp.84-88
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• 2011

Edestin, a major hempseed storage protein from Cheungsam, was isolated to apparent homogeneity by acid precipitation and gel filtration chromatography. The native molecular weight of purified edestin was approximately 300 kDa by Sephacryl S-300 gel filtration. Upon adding 2-mercaptoethanol, the isolated edestin of 56.7 kDa on the non-reduced sodium dodecyl sulfate polyacrylamide gel was converted into subunits, suggesting that the protein might be composed of subunits linked by disulfide bond. Cheungsam edestin was rich in essential amino acids and it has 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The results suggest that Cheungsam edestin might be utilized as a superior antioxidative nutrient.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1999년도 봄 학술발표회 논문집
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• pp.187-194
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• 1999

Laminated rubber bearings are widely used as a key component in seismic isolation of structural systems subjected to earthquake loadings. The combination of rubber layers and reinforcing steel shims makes the bearings conditionally unstable similar to buckling of ordinary columns. The shear flexibility of these short columns can lead to relatively low buckling Toads which may be further reduced when high shear strains are simultaneously imposed As an analytical approach, the area reduction formula has been proposed to account for the reduction in buckling load due to shear, but the degree of conservatism is unknown. In order to complement analytical approaches, a non-linear finite element analysis can be used. In this paper, a numerical study which aims at determining the effect of high shear strain on the critical load of elastomeric bearings is presented. From the load-displacement curve at each specified shear displacement, the buckling load can be obtained using the Southwell procedures. The results obtained are then compared against the theoretical predictions in order to examine the validity and the conservatism of the theoretical formulas.

• 전기학회논문지
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• 제56권12호
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• pp.2240-2246
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• 2007

Optically coupled linear isolation amplifier requires special care to avoid oscillation in actual applications. In this paper methods to analyze the frequency characteristics of the amplifier are proposed to reveal the cause of oscillation and to provide design guidelines. The loop gain of the amplifier obtained through the equivalent circuit model shows that the phase margin is too small to ensure stable operations. Methods to get non-oscillatory response are proposed and the resulting frequency responses are analyzed. The common method adding a small capacitor to the amplifier is shown to degrade the frequency bandwidth. The frequency response of output voltage explains the need of filter capacitor in output stage. The usefulness of the method is verified through experiments.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2010년도 하계학술대회 논문집
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• pp.500-501
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• 2010

본 논문에서는 출력전압레벨수를 증가시키기 위한 방안으로 3의 n승비의 입력전압원을 가지는 비절연방식과 동일한 레벨 형성을 위해 변압기 이차 측을 직렬로 결합시킨 절연 방식의 멀티레벨 인버터의 특성을 비교 분석한다. 두 회로는 동일하게 3의 배수의 전압원을 가감하여 선형적인 출력 전압 레벨을 형성할 수 있다. 다단 변압기를 적용하는 방식은 하나의 입력전압원을 가지고 변압기의 권선비를 3의 배수로 설계하여 출력전압의 순시치가 다단의 출력전압 레벨을 형성하는 개념이며, 제안된 방식은 전압원이 3의 배수 형태로 이루어진 경우 동일한 출력전압 레벨을 생성시킬 수 있는 구조이다. 따라서 제안된 구조가 회로 소자수 측면과 효율 특성에 있어 보다 우수한 특성을 가지게 된다. 두 회로의 비교 분석을 위한 시뮬레이션을 수행하고 타당성을 검증한다.

• 반도체디스플레이기술학회지
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• 제3권2호
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• pp.5-9
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• 2004

Microcircuit fabrication requires precise control of impurities in tiny regions of the silicon. These regions must be interconnected to create components and VLSI circuits. The patterns to define such regions are created by lithographic processes. In order to image features smaller than 70 nm, it is necessary to employ non-optical technology (or next generation lithography: NGL). One such NGL is extreme ultra-violet lithography (EUVL). EUVL transmits the pattern on the wafer surface after reflecting ultra-violet through mask pattern. If particles exist on the blank mask, it can't transmit the accurate pattern on the wafer and decrease the reflectivity. It is important to care the blank mask. We removed the particles on the wafer using focused ion beam (FIB). During removal, FIB beam caused damage the multi layer mask and it decreased the reflectivity. The relationship between particle removal and reflectivity is examined: i) transmission electron microscope (TEM) observation after particle removal, ii) reflectivity simulation. It is found that the image mode of FIB is more effective for particle removal than spot and bar mode.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 1999년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall
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• pp.220-227
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• 1999

The Purpose of this paper is to evaluate the buckling strength of conceptually designed KALIMER reactor vessel. For evaluation of the buckling load buckling load the design equations and the finite element analysis are used. In finite element method the eigenvalue buckling analysis nonlinear elastic buckling analysis using snap-through buckling method and nonlinear elastic-plastic buckling analysis are carried out. the calculated buckling loads of KALIMER reactor vessel using the finite element method are in well agreement with those of the design equations. From the calculated results of buckling load in KALIMER rector vessel it is shown that the plasticity of vessel materials significantly affects the buckling load but the initial imperfection has little effects, In checking the limits of bucking load of KALIMER reactor vessel using the ASME B & PV Section III. Subsection NH the non-seismic isolation design can not satisfy the buckling limit requirements but the seismic isolation design can sufficiently satisfy the requirements.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2001년도 가을 학술발표회 논문집
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• pp.553-560
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• 2001

To substantiate the application of LRB(Laminated Rubber Bearing) to the seismic isolation system, it is necessary to develop a seismic analysis method considering the non-linear behavior of LRBs, which may significantly affect the seismic responses. In this paper, seismic analyses and shaking table tests are carried out for a seismically isolated structure using four LRBs. The parameter equations of seismic isolation frequency are obtained from the shaking table tests and the quasi-static tests of LRB itself to investigate the effects of the LRB characteristics in the prediction of maximum peak acceleration responses by analysis. From the comparison of the maximum peak acceleration responses obtained from numerical analyses and experiments, it is verified that the horizontal stiffness variations of LRB should be carefully considered in seismic analysis to obtain more accurate results.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 B
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• pp.1339-1341
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• 2000

This paper presents a new PWM DC/DC converter with multi-output using single secondary winding, which has two output characteristics of the isolation and non-isolation simultaneously. The proposed converter topology is consisted of the only one switch and single secondary winding. The proposed converter, therefore, has advantages not only low cost but also high power density. Operating principal of the proposed converter topology with conventional ZVT (Zero-Voltage-Transition) is illustrated in detail and the validity of the converter is verified with several interesting simulation results.

• 한국환경보건학회지
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• 제44권2호
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• pp.133-142
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• 2018

Objectives: The pathogenic Vibrios genus denotes halophilic bacteria that are distributed in aquatic environments, including both sea and freshwater. Vibrio cholerae, Vibrio vulnificus, and Vibrio parahaemolyticus are the most important species since they can be potent human pathogens and leading causes of septicemia, wound infections, and seafood borne gastroenteritis. The recent emergence of a potential pandemic clone, V. cholera serotype O1 and the cholera outbreak in South Korea in 2016 indicates the importance of consistent surveillance of pathogenic Vibrio genus within coastal areas. Methods: The present study was undertaken to determine where and how vibrios live in the aquatic environment adjacent to coastal areas of South Korea. For this survey, a total of 838 samples were obtained at 35 different sites in South Korean coastal areas during the period from January 2016 to December 2016. Pathogenic vibrios was determined using the real-time PCR method, and its clones were isolated using three selective plating media. We also monitored changes in seawater and atmospheric temperature, salinity, turbidity, and hydrogen ion concentration at the collection points. Results: The total isolation rates of V. vulnificus, V. cholera (non-pathogenic, non-O1, non-O139 serogroups), and V. parahaemolyticus from seawater specimens in 2016 were 14.2, 13.48, and 67.06%, respectively. Conclusions: The isolation rates of pathogenic vibrios genus showed a positive correlation with temperature of seawater and atmosphere but were negatively correlated with salinity and turbidity.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1034-1043
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• 2014

This study determined the effect of light intensity and photoperiod on the dry cell weight and total amount of carotenoids in four isolates of purple non-sulfur bacteria obtained from shaded and exposed microhabitats of a mangrove ecosystem in Kota Kinabalu, Sabah, Malaysia. The initial isolation of the bacteria was carried out using synthetic 112 medium under anaerobic conditions (2.5 klx) at $30{\pm}2^{\circ}C$. On the basis of colony appearance, cell morphology, gram staining, motility test, and 16S rRNA gene sequencing analyses, all four bacteria were identified as Afifella marina. One of the bacterial isolates, designated as Af. marina strain ME, which was extracted from an exposed mud habitat within the mangrove ecosystem, showed the highest yield in dry cell weight ($4.32{\pm}0.03g/l$) as well as total carotenoids ($0.783{\pm}0.002mg/g$ dry cell weight). These values were significantly higher than those for dry cell weight ($3.77{\pm}0.02g/l$) and total carotenoid content ($0.706{\pm}0.008mg/g$) produced by the isolates from shaded habitats. Further analysis of the effect of 10 levels of light intensity on the growth characteristics of Af. marina strain ME showed that the optimum production of dry cell weight and total carotenoids was achieved at different light intensities and incubation periods. The bacterium produced the highest dry cell weight of 4.98 g/l at 3 klx in 72 h incubation, but the carotenoid production of 0.783 mg/g was achieved at 2.5 klx in 48 h incubation. Subsequent analysis of the effect of photoperiod on the production of dry cell weight and total carotenoids at optimum light intensities (3 and 2.5 klx, respectively) revealed that 18 and 24 h were the optimum photoperiods for the production of dry cell weight and total carotenoids, respectively. The unique growth characteristics of the Af. marina strain ME can be exploited for biotechnology applications.