• Title/Summary/Keyword: Nitrogen sources

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Studies on Takju Brewing with Potatoes (감자를 이용(利用)한 탁주제조(濁酒製造)에 관(關)한 연구(硏究))

  • Kim, S.Y.;Oh, M.J.;Kim, C.J.
    • Korean Journal of Agricultural Science
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    • v.1 no.1
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    • pp.67-81
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    • 1974
  • In order to prepare the mashing materials for "Takju", Korean wine, with potatoes they were steamed, dryed, and pulverized, and their chemical components were analyzed. As a brewing method of Takju with potatoes, general 2nd stage process with Ipkuk and Bunkuk (enzyme sources), commonly used now, was carried out and the effects of preparing conditions of Ipkuk(koji) with potato flour, mashing materials and brewing conditions on the contents of Takju mash and of storing time on the contents of Takju, were investigated and the results obtained were summarized as follows. 1. Chemical components of steamed potatoes and potato flour were Moisture; 76.2, 10.8%, Total sugar; 16.1, 69.8%, Reducing sugar; 3.45, 13.4%, Crude protein; 2.1, 11.3%, Total acid; 0.012, 0.023% and Volatile acid; 0.0012, 0.0025% respectively 2. The most effective preparing conditions of Ipkuk with potato flour were to incubate the potato flour added 40-50% of water for 48 hours by general preparing process of Koji, and liquefying and saccharogenic amylase activities of Ipkuk incubated at above conditions were $D_{40^{\circ}}{^{30{\prime}}}$ 128 W.V. and 13.2 A. U. 3. The effects of various brewing conditions on the contents of Takju mashes wereas follows; 1) Optimum ratio of mashing water and materials for Takju brewing with potato flour was 140ml of water to 60g of flour in 1st stage and 260ml to 140g in 2nd stage. 2) Optimum fermentating times and temperatures for Takju brewing were at $25^{\circ}C$ for 48 hours in 1st stage and at $30^{\circ}C$ for 48 hours in 2nd stage. 3) Optimum amounts of enzyme sources for Takju brewing 20-30% of Ipkuk and 0.5% of Bunkuk in 1st stage and 1.0% of Bunkuk in 2nd stage. 4) Methanol content of the Takju mash brewed with raw potato flour was much more than that with steamed potato flour. 5) Alcohol fusel oil and Formal nitrogen contents of the Takju mash brewed with potato flour were less than that with wheat flour, on the contrary, methanol contents and total acidities of them were showed conversely above. 4. The changes of chemical components and microflora in the mashes during the brewing potato flour Takju were as follows; 1) The accumulation of ethanol followed rapidly in early stage, being the highest at 72 hours (11.9%) 2) Total sugar content of the mash was decreased considerably within 48-72 hours, being 2.62% at 72 hours, and thereafter slowly. 3) Reducing sugar of the mash had a tendency of decreasing, being 0.29% at 48 hours. 4) Total acidity, volatile acidity and Formal nitrogen content of the mash were increased slowly, being 7.30, 0.20, 2.55 at 48 hours. 5) Total cells of yeast appeared the highest in 72 hours ($2.1{\times}10^8$) and thereafter decreased slowly. 6) Total cells of bacteria appeared the highest in 48 hours ($2.4{\times}10^8$) and thereafter decreased or increased slightly. 5. Takju was made from the fermented mash mixed with water to be 6% of alcohol content, and the change of alcohol content, total acidity, total cells of yeast and bateria during the storing at $30^{\circ}C$ were as follows; 1) Alcohol content of Takju was increased slightly at 24 hours (6.2%), and thereafter decreased slowly. 2) Total acidity of Takju was increased gradually, being 6.1 at 72 hours 3) Total cells of yeast and bacteria appeared the highest at 48 hours ($2.3{\times}10^8$, $1.5{\times}10^8$) and thereafter decreased slowly. 6. Alcohol content, total acidity and Formol nitrogen content of the Takju brewed with potato flour Ipkuk or wheat flour Ipkuk and steamed potatoes(1:5) were 9.8-11.3%, 5.8-7.4, 2.5-3.3 respectively, and the color of the Takju was similar to commercial Takju. 7. The results of sensory test for various experimental Takju, showed that the Takjues brewed with the materials combined with wheat flour and steamed potatoes (4:5 or 3.5:7.5) were not significantly different in color, taste and flavor from commercial Takju, However, those with potato flour and wheat flour (1:1 or 7:3) were significantly different from commercial Takju.

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Factors Affecting the Formation of Iodo-Trihalomethanes during Chlorination in Drinking Water Treatment (정수처리에서 염소 처리시 요오드계 트리할로메탄류 생성에 영향을 미치는 인자들)

  • Son, Hee-Jong;Yoom, Hoon-Sik;Kim, Kyung-A;Song, Mi-Jeong;Choi, Jin-Taek
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.8
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    • pp.542-548
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    • 2014
  • Effects of bromide ($Br^-$) and iodide ($I^-$) concentrations, chlorine ($Cl_2$) doses, pH, temperature, ammonia nitrogen concentrations, reaction times and water characteristics on formation of iodinated trihalomethanes (I-THMs) during oxidation of iodide containing water with chlorine were investigated in this study. Results showed that the yields of I-THMs increased with the high bromide and iodide level during chlorination. The elevated pH significantly increased the yields of I-THMs during chlorination. The formation of I-THMs was higher at $20^{\circ}C$ than $4^{\circ}C$, $10^{\circ}C$ and $30^{\circ}C$. In chloramination study, addition of ammonium chloride ($NH_4Cl$) markedly increased the formation of I-THMs. Among the water samples collected from seven water sources including wastewater treatment plant (WWTP) effluent water (EfOM water), prepared humic containing water (HA water) and algal organic matter (AOM) containing water (AOM water), EfOM water generated the highest yields of I-THMs ($12.31{\mu}g/mg$ DOC), followed by HA water ($4.96{\mu}g/mg$ DOC), while AOM water produced the lowest yields of I-THMs ($0.99{\mu}g/mg$ DOC). $SUVA_{254}$ values of EfOM water, HA water and AOM water were $1.38L/mg{\cdot}m$, $4.96L/mg{\cdot}m$ and $0.97L/mg{\cdot}m$, respectively. The I-THMs yields had a low correlation with $SUVA_{254}$ values ($r^2$ = 0.002).

Degradation Ability of Fungicide Myclobutanil by Several Soil Bacteria (수종(數種) 토양세균(土壤細菌)에 의한 살균제(殺菌劑) Myclobutanil의 분해력(分解力))

  • Han, Seong-Soo;Park, Pill-Jae;Jeong, Jae-Hun;Rim, Yo-Sup
    • Korean Journal of Environmental Agriculture
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    • v.15 no.1
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    • pp.25-36
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    • 1996
  • This study was carried out to isolate some bacterial strains which had potentiality of good degrader of fungicides from herbicide free soil and to clarify degradation of a fungicide mycrobutanyl[2-p-chlorophenyl-2-(1H-1,2,4-triazole-1-ylmethyl)-hexanenitrile]. Ten strains of the gram-positive and the gram-negative bacteria were isolated and identified. Most of them vigorously proliferated at 55ppm of mycrobutanil, but the stains were not grown when more than 70ppm of this fungicide were treated Staphylococcus spp. I, Actinobacillus spp. III, and another I of the isolated bacteria degraded more than 35% of the treated mycrobutanil. These three strains could utilize mycrobutanil as nitrogen and carbon sources. Mycrobutanil was rapidly decomposed by these strains when applied once or three times. Tested bacteria gradually increased in growth when mycrobutanil was applied repeatedly. Degradation of mycrobutanil and growth of these bacteria were greater in pH 5.5, and they were high in the order of $28^{\circ}C$ > $18^{\circ}C$ > $38^{\circ}C$.

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The Policy Review and Water Quality Characteristics of National Fishing Harbors and Designated Ports in East Coast of Korea (동해안 국가어항과 지정항만의 수질특성 및 정책적 고찰)

  • Lee, Dae-In;Kim, Gui-Young;Moon, Ju-Hoon;Eom, Ki-Hyuk
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.14 no.4
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    • pp.213-223
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    • 2011
  • The status and changes of water quality of national fishing harbors and designated ports in East Coast of Korea were analyzed to support establishment effective water environmental management. COD (Chemical Oxygen Demand) concentration was satisfied to designated water quality criteria in most areas, but TN (Total Nitrogen) and TP (Total Phosphorus) exceeded the criteria frequently. Also, peak concentration was summer in COD and SS (Suspended Solid), but winter in TP. Eutrophication index of Ganggu and Pohang (old) area were the highest. Pollution index by function of COD, TN, and TP of Ganggu, Pohang, Jumunjin, and Guryongpo was high with gradual increasing recently, on the contrary, that of Samcheok, Imwon, and Chuksan was decreased. Pollution index involving multi-indictors relation to organics and inorganics was necessary for water quality assessment. Designated water quality criteria needed to be improved because the criteria of Jukbyun and Chuksan was applied more strictly compared to the other regions although without difference of environmental characteristics. Furthermore, the criteria notified lately needed to be related to management pollutants from land-based sources. The continuous diagnosis and monitoring on sediment quality within the study area were necessary for prevention of water pollution and eco-friendly disposal of dredged sediment. Especially, monitoring of Designated Ports was implemented partially, however monitoring ratio of National Fishing Har-bors was 7% to whole part. Therefore, systematic and integrated environmental monitoring for ports and harbors with charge of national management was reestablished by strengthening and securing a legal basis.

Incubation Conditions Affecting Bacteriocin Production of Lactobacillus plantarum K11 Isolated from Dongchimi (동치미에서 분리된 Lactobacillus plantarum K11의 박테리오신 생산에 영향을 미치는 배양 조건)

  • Lim, Sung-Mee;Lee, Gun-Ja;Park, Sun-Mee;Im, Dong-Soon
    • Journal of Food Hygiene and Safety
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    • v.23 no.2
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    • pp.113-120
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    • 2008
  • The influence of incubation temperature, pH and media components on bacteriocin production by Lactobacillus plantarum K11 were investigated. The highest activity was observed in MRS broth, but no bacteriocin activity was obtained in TSB. The bacteriocin was produced from the exponential growth phase and its activity also reached a maximum in MRS broth, but then dropped after 16 hr because of degradation by extracellular proteolytic enzymes or exhaustion of medium nutrients. The optimal temperature and pH for production of bacteriocin were $37^{\circ}C$ and pH 7.0 in MRS broth, respectively. The addition of 0.5 or 1.0% glucose and $0.5{\sim}1.5%$ lactose to MRS resulted in the increase of the bacteriocin production. With 0.5% NaCl and $K_2HPO_4$, the activities were significantly higher than that of control, respectively. However, increasing nitrogen sources such as beef extract, casein, and tryptone and salts such as $NH_4PO_4$, $MgSO_47H_2O$, and $MnSO_4H_2O$, had detected a negative influence upon the bacteriocin production. Consequently, because the bacteriocin produced by L. plantarum K11 was affected by various incubation conditions, the bacteriocin activity of L. plantarum K11 applied in food as a novel starter will be dependent on environmental factors such as fermentation conditions and food ingredients.

New Estimates of CH4 Emission Scaling Factors by Amount of Rice Straw Applied from Korea Paddy Fields (볏짚 시용에 따른 벼 재배 논에서의 메탄 배출계수 개발에 관한 연구)

  • Ju, Okjung;Won, Tae-Jin;Cho, Kwang-Rae;Choi, Byoung-Rourl;Seo, Jae-Sun;Park, In-Tae;Kim, Gun-Yeob
    • Korean Journal of Environmental Agriculture
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    • v.32 no.3
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    • pp.179-184
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    • 2013
  • BACKGROUND: Accurate estimates of total direct $CH_4$ emissions from croplands on a country scale are important for global budgets of anthropogenic sources of $CH_4$ emissions and for the development of effective mitigation strategies. Methane production resulted by the anaerobic decomposition of organic compounds where $CO_2$ acts as inorganic electron acceptor. This process could be affected by the addition of rice straw, water management and rice variety itself. METHODS AND RESULTS: Rice (Oryza sativa L. Japonica type, var Samkwangbyeo) was cultivated in four plots: (1) Nitrogen-Phosphorus-Potassium (NPK) ($N-P_2O_5-K_2O$:90-45-57 kg/ha); (2) NPK plus 3 Mg/ha rice straw (RS3); (3) NPK plus 5 Mg/ha rice straw (RS5); (4) NPK plus 7 Mg/ha rice straw (RS7) for 3 years (2010-2012) and the rice straw incorporated in fall (Nov.) in Gyeonggi-do Hwaseong-si. Gas samples were collected using the closed static chamber which were installed in each treated plot of $152.9m^2$. According to application of 3, 5, 7 Mg/ha of rice straw, methane emission increased by 46, 101, 190%, respectively, compared to that of the NPK plot. CONCLUSION(S): We obtained a quantitative relationship between $CH_4$ emission and the amount of rice straw applied from rice fields which could be described by polynomial regression of order 2. The emission scaling factor estimated by the relationship were in the range of IPCC GPG (2000).

Characteristics of Physico-chemical Water Quality Characteristics in Taehwa-River Watershed and Stream Ecosystem Health Assessments by a Multimetric Fish Model and Community Analysis (태화강 수계의 다변수 어류평가 모델 및 군집분석에 의한 이화학적 수질 특성 및 하천 생태건강도 평가)

  • Kim, Yu-Pyo;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.43 no.3
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    • pp.428-436
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    • 2010
  • This study was to evaluate water quality characteristics and ecological health using a mulimetric fish model in Taehwa-River watershed during May~September 2009. The ecological health assessments were based on the Index of Biological Integrity (IBI) using fish community and the multimetric model of Qualitative Habitat Evaluation Index (QHEI). For the study, the models of IBI and QHEI were modified as 8 and 11 metric attributes, respectively. We also analyzed spatial patterns of chemical water quality over the period of 2000~2009, using the water chemistry dataset, obtained from the Ministry of Environment, Korea. Values of BOD and COD averaged $1.7\;mg\;L^{-1}$ (scope: $0.1{\sim}31.8\;mg\;L^{-1}$) and $3.6\;mg\;L^{-1}$ (scope: $0.4{\sim}33\;mg\;L^{-1}$), respectively during the study. Total nitrogen (TN) and total phosphorus (TP) averaged $2.8\;mg\;L^{-1}$ and $96.8\;{\mu}g\;L^{-1}$, respectively, indicating an eutrophic-hypertrophic state. Also, TN and TP showed longitudinal increases toward the downriver reach. In the watershed, QHEI values varied from 67.5 (fair condition) to 164.5 (good condition) by the criteria of US EPA (1993). There was a abruptly decreasing tendency from T9 site in the QHEI values. According to 1st and 2nd surveys of Taewha River, multimetric model values of IBI was averaged 26.1 (n=14) with "good" condition (B) and the spatial variation was evident. Our results suggest that the mainstream sites was getting worse health condition along the river gradient due to inputs of the point and non-point sources from the urban (Ulsan city). Overall, dataset of IBI, QHEI, and water chemistry indicated that the ecological river health showed a downriver decline and the pattern was closely associated with habitat degradations and chemical pollutions as the waters pass through the urban region.

Long-Term Variations of Water Quality in Jinhae Bay (진해만의 장기 수질변동 특성)

  • Kwon, Jung-No;Lee, Jangho;Kim, Youngsug;Lim, Jae-Hyun;Choi, Tae-Jun;Ye, Mi-Ju;Jun, Ji-Won;Kim, Seulmin
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.17 no.4
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    • pp.324-332
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    • 2014
  • In order to reveal the long-term variations of water quality in Jinhae Bay, water qualities had been monitored at 9 survey stations of Jinhae Bay during 2000~2012. The surface and bottom waters concentrations of chemical oxygen demand (COD), dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), and chlorophyll-a (Chl.-a) were higher at the survey stations of Masan Bay than the stations of other Bays. Especially, station 1 which is located at the inner area of Masan Bay had the highest values in the concentrations of COD, DIN, and Chl.-a because there were terrestrial pollutant sources near the station 1 and sea current had not well circulated in the inner area of Masan Bay. In factor analysis, the station 1 also had the highest factor values related to factors which increase organic matters and nutrients in surface and bottom waters of Masan Bay. However, the stations (st.5, st.6, st.7, st.8, and st.9) of other Bays had lower values of the factors. In time series analysis, the COD concentrations of the bottom waters at 8 stations except for station 1 distinctly decreased. However, the COD concentrations of the surface waters showed no distinct decrease trends at all stations. In the concentrations of nutrients (DIN and DIP) of both surface and bottom waters, there were tremendous decrease trends at all stations. Therefore, these distinct decrease trends of the COD in bottom waters and the nutrients in surface and bottom waters of Jinhae Bay could have been associated with water improvement actions such as TPLMS (total pollution load management system).

Effects of Applying Cattle Slurry and Mixed Sowing with Legumes on Productivity, Feed Values and Organic Stock Carrying Capacity of Whole Crop Barley and Rye (액상우분뇨 시용과 콩과작물의 혼파가 청보리와 호밀의 생산성, 사료가치 및 단위면적당 유기가축 사육능력에 미치는 영향)

  • Jo, Ik-Hwan;HwangBo, Soon;Lee, Sung-Hoon
    • Korean Journal of Organic Agriculture
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    • v.18 no.3
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    • pp.419-432
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    • 2010
  • This study was conducted to determine effects of applying cattle slurry and mixed sowing with legumes such as hairy vetch or forage pea on productivity, feed values and organic stock carrying capacity of whole crop barley and rye as winter forage crops, and to obtain organic forages together with higher soil fertility. Experimental plots consisted of 7 treatments, which were non-fertilizer, chemical fertilizer (containing phosphate and potassium: P+K), chemical fertilizer (containing nitrogen, phosphate and potassium: N+P+K), organic fertilizer, cattle slurry, cattle slurry application (mixture with hairy vetch), and cattle slurry application (mixture with forage pea) plots. Each treatment was triplicates, and seven treatments were allocated in a completely randomized block design. For whole crop barley or its mixture crops, annual dry matter (DM), crude protein (CP), and total digestible nutrients (TDN) yields of N+P+K plots were significantly (P<0.05) higher than other plots except for cattle slurry plots. The CP content of barley or its mixture crops was significantly higher tor N+P+K plot (9.8%) and mixture plots with legumes (8.6~9.7%) than those of other treatments. As 450 kg Hanwoo heifers were fed diets included 70% whole crop barley or 70% mixture crops with legumes, mixture plots are capable of raising average 1.7 to 1.8 heads/ha a year. For rye or its mixture crops, annual DM, CP, and TDN yields represented 6.9~7.1, 0.5~0.6 and 4.3~4.4 ton/ha, respectively. The N+P+K plot contained 10.8% CP, which was higher (P<0.05) than all other treatments. In case of 450 kg Hanwoo heifers fed diets included 70% rye or 70% mixture crops with legumes, mixture plots can rear average 1.9 heads/ha a year. When it was considered based on crop yields and organic stock carrying capacity, applying cattle slurry to whole crop barley or rye had the comparable yields and feed values to chemical fertilizer application. Moreover, whole crop barley and rye within cattle slurry plots had a greater combination with hairy vetch and forage pea, respectively, and their mixture crops with legumes had higher crude protein and TDN yields within cattle slurry plots. In conclusion, it would be expected that mixed sowing with legumes in the application of cattle slurry to grass crops could be substituted for imported organic grains as dietary protein sources in feeding organic livestock.

Studies on Production of Heteropolysaccharide by Mutant of Xanthomonas malvacearum (Xanthomonas malvacearum 돌연변이주(突然變異株)의 Heteropolysaccharide 생산성(生産性)에 관(關)하여)

  • Lee, Ke-Ho;Kim, Mi-Sun;Park, Chan-Yung
    • Applied Biological Chemistry
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    • v.30 no.1
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    • pp.77-87
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    • 1987
  • The mutant with high productivity, X. malvacearum SNUF 560-6, was acquired from the X. malvacearum SNUF 560 with low productivity by UV-light irradiation. It was preserved is lyophilized stock culture and it was transferred to PDA slant to maintain viability fortnightly. Fermentations were started by retransfering to MY agar slant from PDA stok culture. The experiments for optimal xanthan gum production were studied in a chemically defined medium. Of the carbon and nitrogen sources tested, 0.4% sucrose medium and 10mM glutamic acid medium yielded the highest xanthan gun production respectively. The addition of 10g/l succinic acid stimulated xanthan gum production. Also 65mM $PO_4\;^{-3}\;(12.6g/l\;KH_2PO_4)$ was effective on xanthan gum production. Finally, medium 1 and medium 2 which have high xanthan gum production potencies were achieved in this stud. The components of medium 1 and medium 2 were as follows: Medium 1 : sucrose 40g/l glutamate 10mM $PO_4\;^{-3}\;54mM\;(KH_2PO_4\;12.65g/l)$ Citrate 2g/l $MgSO_4{\cdot}7H_2O\;0.2g/l$ $H_3BO_3\;0.005/l$ ZnO 0.006/l $FeCl_2{\cdot}6H_2O\;0.0024g/l$ $CaCO_3\;0.02g/l$ Medium 2 : $Sucrose\;40g/l\;(NH_4)_2SO_4\;2g/l$ $PO_4\;^{-3}\;65mM\;(KH_2PO_4\;12.65g/l)$ Succinate 10g/l $MgSO_4{\cdot}7H_2O\;0.02g/l$ $H_3BO_3\;0.06g/l$ ZnO 0.006g/l $FeCl_2{\cdot}6H_2O\;0.0024g/l$ $CaCO_3\;0.02g/l$.

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