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Experimental Analysis of Interactions Among Saprotrophic Fungi from A Phosphorous-Poor Desert Oasis in the Chihuahuan Desert

  • Marini-Macouzet, Constanza;Munoz, Luis;Gonzalez-Rubio, Aldo;Eguiarte, Luis E.;Souza, Valeria;Velez, Patricia
    • Mycobiology
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    • v.48 no.5
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    • pp.410-417
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    • 2020
  • Fungal ecological interactions play a key role in structuring community assemblages. These associations may involve both antagonistic and synergistic relationships, which are commonly influenced by abiotic factors such as nutrient conditions. However, information for extreme, oligotrophic systems remain poor. Herein, interactions among key members of the aquatic transient fungal community (Aspergillus niger, Cladosporium sp., and Coprinellus micaceus) of a low-nutrient freshwater system in the Cuatro Ci enegas Basin, Mexico were studied. Pairwise interaction bioassays were explored in vitro under different nutrient conditions, including carbohydrates-rich, carbohydrates and amino peptides-rich, and low nutrients. Our results indicated that antagonistic patterns prevail among the studied taxa. However, nutrient-dependent changes were observed in Cladosporium sp. shifting to synergy under carbohydrates-rich conditions, suggesting changes in the fungal community composition as a result of nutrient enrichment. Remarkably, our findings contrast with previous work demonstrating mainly synergistic interactions between our tested fungal isolates and co-occurring autochthonous bacteria (Aeromonas spp. and Vibrio sp.) under low-nutrient conditions. This observation may indicate that bacteria and fungi exhibit distinct community-level responses, driven by nutrient conditions. This contributes to the knowledge of fungal community dynamics and interspecific interactions in an oligotrophic ecosystem, highlighting the relevance of nutrient-based shifts and antagonistic interactions in ecosystem dynamics.

Effect of the paper acidity on the cellulolytic activity of fungi (종이의 산성화가 미생물의 분해능에 미치는 영향)

  • Han, Sung-Hee;Lee, Kyu-Shik;Chung, Young-Jae;Lee, Hye-Yun
    • 보존과학연구
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    • s.19
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    • pp.3-22
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    • 1998
  • The effect of pH on degradation of paper by some fungi, which able to degrade cellulose, was investigated. Trichoderma koningii, Aspergillus nigerand Penicillium nigulosum were cultured at $28^{\circ}C$ for 16 days in the selective medium (PH3, PH4, PH5, PH6, PH7, PH8, PH9, PH10, PHC) containing paper as substrate. Each paper was pretreated with each pH buffer (pH 3∼pH 10, D.W.)prior to addition to the selective medium. Enzyme activities in the each culture medium were measured spectroph to metrically using C.M.C., Avicel, PNPG as the substrates for endoglucanase, exoglucanase and $\beta$-glucosidase, respectively. In all experimental fungi, the enzyme activities of PH3 and PH9 medium were usually much higher than those of other experimental groups. However in the PH6medium, enzyme activity was lower than other groups. To analyze the concentration and pattern of protein in the each culture medium, the medium was concentrated by lyophilization. The protein concentration of PH3 and PH9 medium were relatively high (T.koningii; 6.31mg, 6,19mg, A.niger; 1.62mg, 1.96mg, P.nigulosum;2.50mg, 2.73mg, respectively), but that of PH6 was relatively low. The protein pattern of each medium was analyzed by using SDS-PAGE and VDS Image Master Analysis Program. The concentrations of bands in the each lane were usually high at lane2 (PH3) and lane8 (PH9) and low at lane5 (PH6). Therefore, the incresed cellulolytic activity of fungus against acidified paper could be result of structural change and deterioration of paper caused by being acidified.

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Control of Basal Stem Rot Disease in Oil Palm by Supplementation of Calcium, Copper, and Salicylic Acid

  • Bivi, M. Shahul Hamid Rahamah;Paiko, Adamu Saidu;Khairulmazmi, Ahmad;Akhtar, M.S.;Idris, Abu Seman
    • The Plant Pathology Journal
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    • v.32 no.5
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    • pp.396-406
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    • 2016
  • Continuous supplementation of mineral nutrients and salicylic acid (SA) as foliar application could improve efficacy in controlling basal stem rot (BSR) disease in oil palm seedling. It is revealed from the results that the highest disease severity index (58.3%) was recorded in T8 treatments at 9 months after inoculation. The best disease control was achieved by T7 treatments (calcium/copper/SA [Ca/Cu/SA]) (5.0%) followed by T1 (5.5%), T5 (5.8%), T3 (8.3%), T6 (8.3%), T4 (13.3%), and T2 (15.8%) treatments. Continuous supplementation of Ca/Cu/SA was found to be the most effective in controlling the disease and the high performance liquid chromatography results showed the detection of ergosterol at very low concentration in the treated samples. Moreover, the transmission electron microscopy analysis results clearly indicated that T7 treatment was also enhancing lignification, which was responsible for the thickness of the secondary cell walls and middle lamella compared to untreated samples. It was therefore, concluded that continuous supplementation of minerals nutrients and SA could effectively suppress disease severity by reducing ergosterol activity and also improve the process of lignification in the treated plants. Furthermore, this treatment also managed to delay the onset of BSR symptoms and promote the growth of the seedlings and eventually suppress the BSR disease.

Studies on Nitroaniline derivative of 2, 2'-methylene-bis-(3, 4, 6-trichloroacetoxy benzene by Mannich reaction Part I. Synthesis and Antimicrobial activity (2, 2'-methylene-bis-(3, 4, 6-trichloroacetoxy benzene)의 nitroaniline 계 mannich bases에 관한 연구 I. 합성 및 항균성)

  • Yu, Ju-Hyun;Kim, Yu-Sam;Kim, Jong-Ho;Yang, Ryung
    • Korean Journal of Food Science and Technology
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    • v.6 no.1
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    • pp.6-11
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    • 1974
  • Four new compounds; 2,2'-methylene-bis [3, 4, 6-trichloro ${{\beta}-(o-nitroanilino)$ propionoxy} benzene]; m.p. $200{\sim}202^{\circ}C,\;C_{31}H_{22}O_8N_4Cl_6$ 2,2'-methylene-bis [3, 4, 6-trichloro ${{\beta}-(p-nitroanilino)$ propionoxy} benzene]; m.p. $168-170^{\circ}C,\;C_{31}H_{22}O_8N_4Cl_6$ : 2,2'-Methylene-bis [3, 4, 6-trichloro ${{\beta}-(o-chloro-p-nitroanilino)$ propionoxy} benzene]; m.p. $170.5-172.5^{\circ}C,\;C_{31}H_{20}O_8N_4Cl_8$ : 2,2'-Methylene-bis [3, 4, 6-trichloro ${{\beta}-(c-methyl-p-nitroanilino)$ propionoxy} benzene]; m.p. $163-164^{\circ}C,\;C_{33}H_{26}O_8N_4Cl_6$-were synthesized by Mannich reaction from 2,2'-Methylene-bis (3, 4, 6-trichloroacetoxy benzene) and their antimicrobial activities against the microorganisms Staphylococcus aureus, Escherichia coli, Bacillus subtilis Natto, Brevibacterium ammoniagenes, Candida tropicalis, Rhodotorula glutinis, Pseudomonas ovalis, Aspergillus candidus Link, Aspergillus awamori Nakazawa. Aspergillus niger var. Tieghem, Aspergillus usami Sakakuchi, Penicillium notatum-were tested. 2,2'-methylene-bis [3, 4, 6-trichloro ${{\beta}-(o-nitroanilino)$ propionoxy} benzene] showed a strong antimicrobial activity against Bacilus subtilis Natto and Brevibacterium ammoniagenes.

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Enzymatic Milling Process for Barley Flour Preparation (보리의 효소적(酵素的) 제분(製粉) 및 이용(利用)에 관한 연구)

  • Kwon, Tai-Wan;Ahn, Byung-Yoon;Choi, Weon-Sang;Cheigh, Hong-Sik
    • Korean Journal of Food Science and Technology
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    • v.18 no.3
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    • pp.197-203
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    • 1986
  • An enzymatic flour milling process for barley into three major fractions (barley flour, bran-crease-germ and water solubles) was studied. Carbohydrate and protein of barley endosperm could be efficiently solubilized by the digestion process of partially pearled barley with enzymes. Bran, crease and germ were removed from hydrolyzate by filtering through 30-40mesh sieves. And then filtered product was separated into fractions by sedimentation or centrifugation. The most effective digestion of the barley was obtained by the enzyme with higher activities of glucanase and protease under such conditions as barley-water ratio, 1:1.5(W/V) and temperature at $45^{\circ}C$. Total flour yield recovered was approximately 73-76% of the barley, and the portions recovered as bran-crease-germ and water solubles were about 3.6 and 15.8%, respectively.

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Decay Resistance and Anti-mold Efficacy of Wood Treated with Fire Retardants (난연처리 목재의 방미 및 방부성능)

  • Son, Dong Won;Kang, Mee Ran;Lee, Dong-Heub;Park, Sang-Bum
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.6
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    • pp.559-565
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    • 2013
  • This study evaluated the ability of white and brown rot fungi to decompose fire retardant-treated wood by measuring mass loss. Anti efficacy of FRT against sapstain and mold fungi was evaluated. Wood was treated with liquid sodium silicate and boric acid, ammonium borate, di-ammonium phosphate. Retardant treated wood was then subjected to fungal decay resistance tests performed according to KS standard method using a brown-rot fungus, Fomitopsis palustris and white rot fungus Trametes versicolor. Aspergillus niger, Penicillium funiculosum, Rhizopus nigricans, Aureobasidium pullulans, Tricoderma virede fungi were used anti-sapstain and mold test. Boron and phosphorus chemicals used in this study increased the resistance of fire retardant treated wood against both fungal attack. Anti mold and sapstain efficacy of the fire retardant treated wood was excellent but there were difference depend on mold. After the liquid sodium silicate treatment, the second chemical treatment process could lead chemical fixation into wood, which effects decay resistance.

Counterterrorism in the Lake Chad Basin Commission and Its Effects on Tenure

  • Gwaya, Kwalar Raymond;Ham, Myungsik
    • Korea and Global Affairs
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    • v.3 no.2
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    • pp.155-182
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    • 2019
  • This essay examines the relationship between counterterrorism within the Lake Chad Basin Commission hereinafter referred to as LCBC and its effects on leaders' tenure in office. The countries of the LCBC, an intergovernmental institution promoting regional integration in the sub-region, established the Multinational Joint Task Force (MNJTF) primarily to maintain peace and security. In April 2012 the mandate of this structure was reinvigorated and broadened to manage the ongoing menace perpetrated by the Islamist terrorist group, Boko Haram in the sub-region. The political consequences, however, has not been identical for leaders of the member states of the LCBC participating in this counterterrorism operation. This essay primarily relies on secondary data derived from available public data sets in explaining this phenomenon. The phenomenon of leaders in Nigeria, Cameroon, Niger and Chad between 2010 and 2018 is used to examine the relationship between counterterrorism in the Lake Chad Basin Commission and its effects on political survival. Results suggest that leaders of highly democratic countries are more likely to lose office and held accountable for either perceived inaction or failure in the counterterrorism operation than their counterparts in autocratic states.

Butterfly Diversity, Distribution, and Abundance in the University of Port Harcourt River State, Nigeria

  • Efenakpo, Ogaga Dean;Zakka, Usman;Omanoye, Dokubo ThankGod
    • Journal of Forest and Environmental Science
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    • v.37 no.3
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    • pp.243-250
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    • 2021
  • Information on butterflies in the Niger Delta Region is relatively scanty because monitoring of their activities and distribution has not been fully documented and these are paramount to establish their conservation protocols. The study therefore aimed at evaluating the species richness distribution pattern, and relative abundance of butterflies in the University of Port Harcourt. The University Park was purposively selected for this study. The park was stratified into three different habitat types (secondary forest, farmland, and residential/garden). A sampling of each stratum was done three times; twice in the morning between 8:00 am and 12:00 pm as well as once in the evening between 4:00 pm and 6:00 pm along a 100 m line transects for 16 months (May 2017-August 2018). Data collected were analyzed for species diversity indices, evenness, and similarity index. A total of 1,932 individual butterflies belonging to 28 species, 18 genera, and five 5 families were identified in the study area. Species diversity and evenness were higher on farmland (1-D=0.909; H'=2.615; E=0.833), and least in the residential area/gardens (1-D=0.744; H'=1.975; E=0.659). The family Nymphalidae (39.0%) had the highest number of species occurrence while Hesperidae (0.4%) had the lowest composition. Anthene larydas (24.12%) had the highest relative abundance in the entire study area, followed by Acraea serena (17.49%). The study, therefore, recommends continuous monitoring of butterfly species diversity and composition also in other regions.

Inhibitory effects of ultraviolet-C light and thermal treatment on four fungi isolated from pig slaughterhouses in Korea

  • Lee, Eun-Seon;Kim, Jong-Hui;Kang, Sun Moon;Kim, Bu-Min;Oh, Mi-Hwa
    • Journal of Animal Science and Technology
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    • v.64 no.2
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    • pp.343-352
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    • 2022
  • Pig slaughterhouses harbor high humidity because of the necessary cleaning that takes place simultaneously with slaughter, which facilitates the existence of mold. Due to the enclosed space, there are several limitations to the control of mold growth with respect to cleaning, ventilation, and drying. In this study, the prevalence of fungi was investigated in four pig slaughterhouses in Korea. Four fungi (Aspergillus niger, Penicillium commune, Penicillium oxalicum, and Cladosporium cladosporioides) were detected with the highest frequency. These four strains were subjected to various treatments to reduce their growth. The fungi were inoculated onto stainless steel (SS) chips and treated with ultraviolet (UV)-C irradiation and hot water. Individual treatments with UV-C (15, 30, 90, 150, 300, and 600 mJ/cm2), and hot water (60, 65, 70, and 83℃) were performed to sanitize the SS chips. Simultaneous cleaning with 60℃ hot water and more than 150 mJ/cm2 of UV-C reduced the fungal incidence by > 6.5 Log from 6.6-7.0 Log CFU/cm2 (initial count). Our results demonstrate that a combined treatment of UV-C and hot water is the most economical and convenient way to prevent microbiological contamination of small tools (such as knives and sharpeners) and steel surfaces in slaughterhouses.

Characteristics of Ocean Environmental Factors and Community Structure of Macrobenthos around Munseom, Jeju Island, Korea (제주도 문섬 주변해역 해양환경특성 및 저서무척추동물의 군집구조)

  • Ko, Joon-Chul;Koo, Jun-Ho;Yang, Moon-Ho
    • The Korean Journal of Malacology
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    • v.24 no.3
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    • pp.215-228
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    • 2008
  • This study was performed to find out the community structure of macrobenthos and the environmental factors around Munseom coastal water in Jeju Island from June to December, 2007. Mean temperature and mean salinity were $19.6^{\circ}C$ and 34.03 psu, indicating stable water masses. Concentrations of DO, COD and SS were as low as those in the standard sea-water-quality classes I. Chlorophyll a concentrations ranged from 0.40 to 1.09 mg/L (mean, 0.69 mg/L), and higher concentration was observed in December rather than in June due to a blooming in winter. The mean concentration (their ranges are in parentheses) of nitrate, phosphate, and silicate were 0.15 mg/L (0.073-0.264 mg/L), 0.01 mg/L (0.004-0.011 mg/L) and 0.23 mg/L (0.039-0.464 mg/L), respectively. The values were higher near the Munseom due to influxes from the land. Of the 112 species that were identified, 40 species (36.0%) were Mollusca; 33 (28.8%) were Cnidaria; 13 (11.7%) were Porifera; 8 (7.2%) were Arthropoda and 17 species (15.3%) were others including Echinodermata. Density and biomass were estimated to be 4,340 individual/$m^2$ and $53,107.8\;gwwt/m^2$ respectively. Anthozoa was the most dominant group in abundance ($2,132\;ind./m^2$) as well as in the number of species, whereas Cnidaria was predominant in biomass ($37,630.9\;gwwt/m^2$). The dominant species were Dendronephthya gigantea, Scleronephthya gracillimum, Anthoplexaura dimorpha, Dendronephthya castanea, Thecocarpus niger, Dendronephthya spinulosa, Dendronephthya putteri, and Acabaria bicolor in the depth of 10-30 m. The seasonal variations of the number of individual and biomass of Cnidaria and Mollusca were observed. There was a slow increment in June, a decrement in October, and a drastic increasing in December. The biodiversity, evenness and richness index in surveyed stations were 2.715-3.413 (H'), 0.758-0.851(E'), and 5.202-8.720 (R) respectively. The dominance index was the highest in station M2 and the lowest in station M1.

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