Enzymatic Milling Process for Barley Flour Preparation

보리의 효소적(酵素的) 제분(製粉) 및 이용(利用)에 관한 연구

  • Kwon, Tai-Wan (Division of Biological Science and Engineering, Korea Advanced Institute of Sci. and Technol.) ;
  • Ahn, Byung-Yoon (Department of Biochemistry, University of Minnesota) ;
  • Choi, Weon-Sang (Division of Biological Science and Engineering, Korea Advanced Institute of Sci. and Technol.,) ;
  • Cheigh, Hong-Sik (Department of Food Sci. and Nutrition, Pusan National University)
  • 권태완 (한국과학기술원 생물공학부) ;
  • 안병윤 (미네소타대학교 생화학과) ;
  • 최원상 (한국과학기술원 생물공학부) ;
  • 최홍식 (부산대학교 식품영양학과)
  • Published : 1986.06.01

Abstract

An enzymatic flour milling process for barley into three major fractions (barley flour, bran-crease-germ and water solubles) was studied. Carbohydrate and protein of barley endosperm could be efficiently solubilized by the digestion process of partially pearled barley with enzymes. Bran, crease and germ were removed from hydrolyzate by filtering through 30-40mesh sieves. And then filtered product was separated into fractions by sedimentation or centrifugation. The most effective digestion of the barley was obtained by the enzyme with higher activities of glucanase and protease under such conditions as barley-water ratio, 1:1.5(W/V) and temperature at $45^{\circ}C$. Total flour yield recovered was approximately 73-76% of the barley, and the portions recovered as bran-crease-germ and water solubles were about 3.6 and 15.8%, respectively.

부분도정한 보리를 미생물효소(Asp. niger 및 oryzae)에 의한 분해반응 및 미세 고형분의 용출, 여과, 침강 또는 원심분리, 건조 등의 과정을 거쳐 제분할 때, 보리가루 획분$(73{\sim}76%)$, 맥강, 배아, 종구등의 잔유물 획분(3.6% 내외) 그리고 가용성물질 획분 등으로 분리하여 얻을 수 있었다. 이 때 가장 좋은 효소반응 조건은 온도 $45^{\circ}C$, 보리와 물의 비율 1 : 1.5(w/v)였고, ${\alpha}-a-mylase$역가 보다는 glucanase 와 Protease의 역가가 높은 효소를 사용할 때 더 좋은 효과를 보였다. 그리고 얻어진 세가지 종류 획분의 성분 조성은 단백질, 지방, 섬유소 등에서 각각 현저한 차이를 보여, 각 획분의 특성별 구분 이용이 가능하였다.

Keywords