• Title/Summary/Keyword: Nickel tolerance

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Nickel Tolerance and the Complexing Role of Histidine in Raphanus sativus (무 유식물에서의 니켈내성과 히스티딘의 작용)

  • Kim, Tae-Yun;Hong, Jeong-Hui
    • Journal of Environmental Science International
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    • v.13 no.8
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    • pp.711-719
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    • 2004
  • The effect of nickel (Ni) on growth and some tolerance strategies with regard to heavy metal tolerance mechanism was investigated in radish (Raphanus sativus) seedlings. The protective effect of histidine on nickel stress conditions was also monitored. The seedling growth decreased with an increase in metal concentrations. The inhibitory effect was more pronounced in the root elongation than in the shoot elongation. Increasing Ni supply showed a progressive increase of Ni concentrations in the roots and shoots. Ni content was higher in the shoots than in the roots. In the presence of nickel, radish exhibited an antioxidative defense mechanism, as evidenced by the elevated malondialdehyde(MDA), showing that nickel is an efficient inducer of lipid peroxidation. Exposure of radish to elevated concentrations of nickel was accompanied by an increase in the proline content. Supplemental histidine in the presence of Ni ameliorated metal-induced growth inhibition and lipid peroxidation. Combinations of Ni and histidine resulted in a significant decline in proline content compared with Ni stress alone, indicating that histidine may provide protection against the adverse effect of Ni stress. From the results it is suggested that histidine is an efficient chelator by complexing metal ion within the plant and may playa role in nickel tolerance implicated in metal detoxification.

Alteration of macronutrients, metal translocation and bioaccumulation as potential indicators of nickel tolerance in three Vigna species

  • Ishtiaq, Shabnam;Mahmood, Seema;Athar, Mohammad
    • Advances in environmental research
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    • v.3 no.1
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    • pp.71-86
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    • 2014
  • Macronutrients ($Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$), yield and yield components, bioaccumulation and translocation of metal in plant parts of three Vigna species (V. cylindrica, V. mungo, V. radiata) were evaluated at 0, 50, 100 and $150mgkg^{-1}$ soil of Nickel (Ni). A marked inhibition (p < 0.001) in the distribution of various macronutrients was noticed in these Vigna species except for $Mg^{2+}$ content of the shoot and leaves. Similarly, all species retained more $Ca^{2+}$ in their roots (p < 0.05) as compared to the aerial tissues. Ni induced a drastic decline (p < 0.001) for various yield and yield attributes except for 100 seed weight. Toxicity and accumulation of Ni in plant tissues considerably increased in a concentration dependent manner. Vigna species signify an exclusion approach for Ni tolerance as both bioaccumulation factor (BF) and translocation factor (TF) were less than 1.0. The Ni content of plants being root > shoot > leaves > seeds. Scoring for percentage stimulation and inhibition (respective to control) at varying levels of Ni revealed tolerance of the species in an order of V. radiata > V. cylindrica > V. mungo. The acquisition of Ni tolerance in V. radiata seems to occur through an integrated mechanism of metal tolerance that includes sustainable macronutrients uptake, stronger roots due to greater deposition of $Ca^{2+}$in the roots, restricted transfer of Ni to above ground tissues and seeds as well as exclusion capacity of the roots to bind appreciable amount of metal to them. Thus, metal tolerant potential of V. radiata could be of great significance to remediate metal contaminated soil owing lesser impact of Ni on macro-nutrients, hence the yield.

Analysis on Monitoring Results of Korean Soil Monitoring Network (토양측정망 운영 결과 분석 연구)

  • Jeong, Seung-Woo
    • Journal of Soil and Groundwater Environment
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    • v.15 no.2
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    • pp.18-23
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    • 2010
  • Usability of soil quality monitoring network for ascertaining soil quality changes was evaluated by analysing soil quality monitoring results. Tolerance limits of soil quality monitoring results from 1997 to 2007 were calculated and compared with Korean soil quality standards. This study determined that soil quality was changed if the upper 95% tolerance limit value was greater than the soil quality standard. Fluoride most frequently exceeded the soil quality standard and nickel, zinc, arsenic, copper, lead and cadmium were followed. Analysis on land use showed that tolerance limits of industrial land use most frequently exceeded the soil quality standards and residential, road and various land uses then frequently exceeded. Tolerance limits of land uses expecting high contaminant loads frequently exceeded the soil quality standards. This fact imply that the soil quality monitoring network generates reasonable data to represent change in Korean soil quality. This study also suggested that representative sampling from well identified points should be done to improve data reliability and accurately ascertain soil quality changes.

Studies on nickel uptake in transgenic Arabidopsis thaliana introduced with TgMTP1 gene encoding metal tolerance protein (TgMTP1 과발현 애기장대에서 Nickel 흡수 연구)

  • Kim, Donggiun
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.409-413
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    • 2015
  • To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both $TgMTP_1$ (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that $TgMTP_1$-overexpressing plants may be useful for phytoremediation of soil.

Crystallization of a-Si Induced by Ni-Si oxide source

  • Meng, Z.;Liu, Z.;Zhao, S.;Wu, C.;Wong, M.;Kwok, H.;Xiong, S.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.985-988
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    • 2008
  • Metal induced crystallization of a-Si with a source of Ni/Si oxide was studied. Its mechanism to induce crystallization was discussed. It was found that new source behaves an effect of self-released nickel and reducing nickel residua, so can provide a wider process tolerance; improve the uniformity and stability of TFTs.

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Characterization of artificially induced zinc-tolerant yeast mutants (아연 저항성 갖는 인위적으로 유도된 효모 돌연변이체의 특성)

  • Lee, Sangman
    • Journal of Applied Biological Chemistry
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    • v.60 no.2
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    • pp.113-117
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    • 2017
  • Bioremediation is a technique using microorganisms to clean up contaminated pollutants including heavy metals. It is well known that yeasts have a high capacity to remove a wide range of metals by biosorption. Therefore, this study was focused on to obtain yeast mutant that has strong tolerance to zinc (Zn), one of representative heavy metals. The Zn resistant yeast mutant (ZnR) was induced and isolated by growing yeast cells in media containing 1 mM $ZnCl_2$ and gradually increasing the concentration until 80 mM $ZnCl_2$, in which cells were adapted and survived. The induced ZnR cells showed strong tolerance to Zn stress compared with control cells. Moreover, the ZnR cells showed increased tolerance to cadmium and nickel stress but decreased tolerance to copper stress. The increased tolerance of ZnR cells to Zn stress was due to mutation of genes. This study can be useful in bioremediation of heavy metals as the metal tolerant microorganism was artificially induced in short time.

Toxic Effects on Lethal Concentration 50%, Heamatological Parameters and Plasma Components of Crucian carp, Carassius carassius by Acute Waterborne Nickel Exposure (수인성 니켈 급성 노출이 붕어, Carassius carassius의 반수치사농도, 혈액학적 성상 및 혈장 성분에 미치는 독성 영향)

  • Chang-Hoon, Joo;A-Hyun, Jo;Su-Min, Hong;Ji-Ho, Jeong;Yun-A, Ryu;Seock-Won, Jo;Jae-Hee, Song;Jun-Hwan, Kim
    • Journal of Marine Life Science
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    • v.7 no.2
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    • pp.102-112
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    • 2022
  • Crucian carp, Carassius carassius (Weight 39.7±3.1 g, Length 14.8±0.5 cm) were exposed to waterborne nickel at 0, 10, 20, 40, 80 and 160 mg Ni2+/l. The lethal concentration 50 (LC50) of C. carassius exposed to waterborne nickel was 117.69 mg Ni2+/l. In hematological parameters, RBC counts was significantly increased at 48 hours, whereas a significant decrease was observed at 96 hours. The MCV and MCH were significantly increased in the concentration of 80 mg Ni2+/l at 96 hours. The plasma components such as calcium, magnesium, glucose, cholesterol, total protein, AST, ALT and ALP were significantly changed by waterbonre nickel exposure. The results of this study suggest that the nickel exposure to C. carassius affects the survival rates, hematological parameters and plasma components as toxicity

Design and Fabrication of Micro Combustor (III) - Fabrication of Micro Engine by Photosensitive Class - (미세 연소기 개발 (III) - 감광 유리를 이용한 마이크로 엔진의 제작 -)

  • Lee, Dae-Hoon;Park, Dae-Eun;Yoon, Joon-Bo;Yoon, Eui-Sik;Kwon, Se-Jin
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.12
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    • pp.1639-1645
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    • 2002
  • Micro engine that includes Micro scale combustor is fabricated. Design target was focused on the observation of combustion driven actuation in MEMS scale. Combustor design parameters are somewhat less than the size recommended by feasibility test. The engine structure is fabricated by isotropic etching of the photosensitive glass wafers. Electrode formed by electroplating of the Nickel. Photosensitive glass can be etched isotropically with almost vertical angle. Bonding and assembly of structured photosensitive glass wafer form the engine. Combustor size was determined to be 1 mm scale. Movable piston is engraved inside the wafer. Ignition was done by nickel spark plug which was electroplated with thickness of 40 ${\mu}{\textrm}{m}$. The wafers were bonded by epoxy that resists high temperature. In firing test due to the bonding method and design tolerance pressure buildup by reaction was not confirmed. But ignition, flame propagation and actuation of micro structure from the reaction was observed. From the result basement of design and fabrication technology was obtained.

Removal of mid-frequency error from the off-axis mirror

  • Kim, Sanghyuk;Pak, Soojong;Jeong, Byeongjoon;Shin, Sangkyo;Kim, Geon Hee;Lee, Gil Jae;Chang, Seunghyuk;Yoo, Song Min;Lee, Kwang Jo;Lee, Hyuckee
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.2
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    • pp.103-103
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    • 2014
  • Manufacturing of lens and mirror using Diamond Turning Machine (DTM) offers distinct advantages including short fabrication time and low cost as compared to grinding or polishing process. However, the DTM process can leave mid-frequency error in the optical surface which generates an undesirable diffraction effect and stray light. The mid-frequency error is expected to be eliminated by mechanical polishing after the DTM process, but polishing of soft surface of ductile aluminum is extremely difficult because the polishing process inevitably degrades the surface form accuracy. In order to increase its surface hardness, we performed electroless nickel plating on the surface of diamond-turned aluminum (Al-6061T6) off-axis mirrors, which was followed by the 6-hour-long baking process at $200^{\circ}C$ for improving its hardness. Then we polished the nickel plated off-axis mirrors to remove the mid-frequency error and measured polished mirror surfaces using the optical surface profilometer (NT 2000, Wyko Inc.). Finally, we ascertained that the mid-frequency error on the mirror surface was successfully removed. During the whole processes of nickel plating and polishing, we monitored the form accuracy using the ultra-high accurate 3-D profilometer (UA3P, Panasonic Corp.) to maintain it within the allowable tolerance range (< tens of nm). The polished off-axis mirror was optically tested using a visible laser source and a pinhole, and the airy pattern obtained from the polished mirror was compared with the unpolished case to check the influence of mid-frequency error on optical images.

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