• Journal of Mushroom
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• v.20 no.3
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• pp.178-182
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• 2022

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.

• Journal of Mushroom
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• v.19 no.4
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• pp.316-321
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• 2021

Ligninolytic enzymes were produced by Pleurotus ostreatus No.42, cultivated in a new kind of bioreactor that has a rotating draft tube with a helical ribbon. Maximum laccase (Lac) production (about 8,200 U/bioreactor) was reached after 3 days of incubation, then production decreased. Production of manganese peroxidase (MnP) in this fermenter reached a maximum level of about 8,400 U/bioreactor after 6 days of incubation. Lignin peroxidase (LiP) was not detected under these growth conditions. These results indicate that the rotary draft tube bioreactor (RTB) is compatible with large scale production of ligninolytic enzymes. MnP produced under these fermentation conditions was purified via a multistep process that included chromatography on Sepharose CL-6B, prep grade Superdex 75, and Mono-Q. This major isoenzyme was confirmed to have an apparent molecular weight of 36,400 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and its isoelectric point (IEF) was determined to be 3.95. N-terminal sequencing of the major isoenzyme from this fermentation was identical to that reported for an MnP3 isoenzyme isolated under different cultivation conditions, including stationary and shaking culture.

• The Journal of the Korea Contents Association
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• v.22 no.2
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• pp.125-134
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• 2022

Due to the development of smartphone technology, as of 2020, 91.9% of people use the Internet[1] to frequently acquire information through websites and mobile apps. As the number of homepages in charge of providing information is increasing every year, the number of applications for web vulnerability diagnosis, which diagnoses the safety of homepages, is also increasing. In the existing web vulnerability check, the number of diagnostic personnel should increase in proportion to the number of homepages that need diagnosis because the diagnosticians manually test the homepages for vulnerabilities. In reality, however, there is a limit to securing a web vulnerability diagnosis manpower, and if the number of diagnosis manpower is increased, a lot of costs are incurred. To solve these problems, an automatic diagnosis tool is used to replace a part of the manual diagnosis. This paper explores a new method to expand the current automatic diagnosis range. In other words, automatic diagnosis possible items were derived by analyzing the impact of web vulnerability diagnosis items. Furthermore, automatic diagnosis identified possible items through comparative analysis of diagnosis results by performing manual and automatic diagnosis on the website in operation. In addition, it is possible to replace manual diagnosis for possible items, but not all vulnerability items, through the improvement of automatic diagnosis tools. This paper will explore some suggestions that can help improve plans to support and implement automatic diagnosis. Through this, it will be possible to contribute to the creation of a safe website operating environment by focusing on the parts that require precise diagnosis.

• Journal of Convergence for Information Technology
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• v.12 no.5
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• pp.194-201
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• 2022

This study purpose was to know the effects 30 aged females forehead ms by SUKI intervention(4weeks). Total tested group were 18 persons, It used for SUKI intervention of SUKI process C1, SUKI process C2, SUKI process C3, SUKI process C4 were adopted three times a week in 4 weeks. The research conclusion like this. EG was a significant difference in forehead ms. Therefore, to maintaining elasticity on forehead ms. showed as some research of SUKI intervention effects for the forehead ms(p<.05). In this study, even though limited, it was judged that wrinkles due to a decrease in elasticity of the forehead ms located on the upper surface of a woman's face in her 30s could have a profound effect on women's external appearance, so SUKI intervention was applied. In addition, the role of the elastic on the forehead ms was to suggest an alternative semester method that can effectively control the management of external appearance by managing which it suitable for the life cycle of women in their 30s. In conclusion, we hope that in the future, various experiments will be used as new research data on how to prevent females facial skin beauty and wrinkles and help improve elasticity of facial ms around the face.

• International Journal of Computer Science & Network Security
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• v.22 no.11
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• pp.141-148
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• 2022

The development of the education system and the labor market today requires new conditions for unification and functioning, the introduction of an innovative culture in the field of Education. The construction of modern management of innovative development of a higher education institution requires consideration of the existing theoretical, methodological and practical planes on which its formation is based. The purpose of the article is to substantiate the modern paradigm of organizing the mechanism of managing the innovative development of higher education institutions. Innovation in education is represented not only by the final product of applying novelty in educational and managerial processes in order to qualitatively improve the subject and objects of management and obtain economic, social, scientific, technical, environmental and other effects, but also by the procedure for their constant updating. The classification of innovations in education is presented. Despite the positive developments in the development of Education, numerous problems remain in this area, which is discussed in the article. The concept of innovative development of higher education institutions is described, which defines the prerequisites, goals, principles, tasks and mechanisms of university development for a long-term period and should be based on the following principles: scientific, flexible, efficient and comprehensive. The role of the motivational component of the mechanism of innovative development of higher education institutions is clarified, which allows at the strategic level to create an innovative culture and motivation of innovative activity of each individual, to make a choice of rational directions for solving problems, at the tactical level - to form motives for innovative activity in the most effective directions, at the operational level - to monitor the formation of a system of motives and incentives, to adjust the directions of motivation. The necessity of the functional component of the mechanism, which consists in determining a set of steps and management decisions aimed at achieving certain goals of innovative development of higher education institutions, is proved. The monitoring component of the mechanism is aimed at developing a special system for collecting, processing, storing and distributing information about the stages of development of higher education institutions, prediction based on the objective data on the dynamics and main trends of its development, and elaboration of recommendations.

• Journal of the Korea Society of Computer and Information
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• v.27 no.10
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• pp.163-173
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• 2022

This study attempted to categorize the lower body torso type and investigated its characteristics for women in their 40s, 50s, and 60s, who increase the frequency of incontinence. This study analyzed the 8th human dimension survey data of Korean Agency for Technology and Standards. The data were analyzed by SPSS 26.0 program. It was analyzed that the height item of the lower body torso decreased, the width item widen as the age increases, and the waist and abdomen circumference of the circumference item increased and the hip circumference decreased. Body length decreased with age. The components of the lower torso were classified into the lower torso horizontal factor, height factor, lower factor, and vertical factor. The lower body torso type was classified into a long inverted triangular body type, a short and high body type, a body fat body type, and a low triangular body type. It was analyzed that type 3 was the largest in the horizontal factor and height factor, and type 1 was the largest in the vertical factor and the lower part factor. A new drafting method was required in setting the horizontal part of the incontinence panty, the front and the back length.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.32 no.5
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• pp.1019-1034
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• 2022

Malware attacks become more prevalent in the hyper-connected society of the 4th industrial revolution. To respond to such malware, automation of malware detection using artificial intelligence technology is attracting attention as a new alternative. However, using artificial intelligence without collateral for its reliability poses greater risks and side effects. The EU and the United States are seeking ways to secure the reliability of artificial intelligence, and the government announced a reliable strategy for realizing artificial intelligence in 2021. The government's AI reliability has five attributes: Safety, Explainability, Transparency, Robustness and Fairness. We develop four elements of safety, explainable, transparent, and fairness, excluding robustness in the malware detection model. In particular, we demonstrated stable generalization performance, which is model accuracy, through the verification of external agencies, and developed focusing on explainability including transparency. The artificial intelligence model, of which learning is determined by changing data, requires life cycle management. As a result, demand for the MLops framework is increasing, which integrates data, model development, and service operations. EXE-executable malware and documented malware response services become data collector as well as service operation at the same time, and connect with data pipelines which obtain information for labeling and purification through external APIs. We have facilitated other security service associations or infrastructure scaling using cloud SaaS and standard APIs.

• Journal of Korean Library and Information Science Society
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• v.54 no.1
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• pp.45-68
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• 2023

This study aims to investigate the awareness and experiences of domestic researchers regarding data publishing, which has been recognized as a new channel of data sharing as scholarly communication evolves in the open science environment. A survey is conducted among researchers from five government-funded research institutes in the field of science and technology and members of the GeoAI Data Society to confirm the awareness of data publishing. As a result of the study, domestic researchers recognized providing explanations for data, stable access to data, citation, and quality assurance through peer review as the advantages of data journals. On the contrary, a low level of recognition for data paper as one of the research outputs was presented. With regard to the properties of data publication, the respondents answered that the data description, metadata description, and permanent identifiers are highly related, however, their recognition of the relation between the properties of data publication and the data submission to a repository and data peer review was relatively low. Finally, to expand the data publication, the need for the development of an editorial system that supports data paper peer review and cross-linking to a data repository as well as the development of a repository that supports data citation was identified. This study on the domestic researchers' experience and awareness of data publishing can provide insights for the implementation of data publishing services and infrastructure in the future.

• Smart Media Journal
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• v.12 no.2
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• pp.27-35
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• 2023

Currently, online user authentication is perform using joint certificates issued by accredited certification authorities and simple certificates issued by private agency. In such a PKI(Public Key Infrastructure) system, various cryptographic technologies are used, and in particular, digital signatures are used as a core technology. The digital signature scheme is equally used in DID(Decentralized Identity), which is attracting attention to replace the existing centralized system. As such, the digital signature-based user authentication used in current online services is also applied in the metaverse, which is attracting attention as the next-generation online world. Metaverse, a compound word of "meta," which means virtual and transcendent, and "universe," means a virtual world that includes the existing online world. Due to various developments of the metaverse, it is expted that new authentication technologies including biometric authentication will be used, but existing authentication technologies are still being used. Therefore, in this study, we study digital signature scheme that can be efficiently used for user authentication in the developing metaverse. In particular, we experimentally analyze the effectiveness of ECDSA, which is currently used as a standard for digital signatures, and Schnorr signatures, which can quickly verify a large amount of signatures.

• Journal of Life Science
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• v.33 no.2
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• pp.216-226
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• 2023

Since nylon 66, a polyamide resin, has been developed and applied to toothbrush bristles, plastic consumption has increased rapidly every year, along with the increase in the amount of plastic discarded. Among the various forms of plastic debris produced by the decomposition of plastics, microplastics with a size of less than 5 mm are widely distributed in the environment, which poses a threat not only to the environment but also to animals and humans. The pathway through which microplastics enter the human body is known as ingestion by water and food, inhalation from air, and skin contact. Microplastics introduced into the human body affect human health. Recently, food-related studies have begun to be reported among microplastics-related studies, and analyses of the presence of microplastics in processed foods, such as canned foods, dried seaweed, beverages, beer, milk, sugar, and honey, are underway. Here, we present trends in the production and consumption of plastics, the generation of microplastics, the route of human inflow and human risk, and the microplastics present in processed foods, which are limited but have recently been reported. Thus far, studies on microplastics and risk assessment in processed foods have been insufficient, but microplastics are gradually being recognized as a factor that affects the environment and food. Future studies are expected to have implications for regulations regarding microplastics present in processed foods.