• Mass Spectrometry Letters
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• v.12 no.3
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• pp.66-75
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• 2021

Interleukin-4 (IL-4) and IL-13 are cytokines secreted by immune cells. Cytokines induce the proliferation of macrophages or promote the differentiation of secretory cells. The initiation and progression of allergic inflammatory diseases, such as asthma, are dependent on cytokines acting through related receptor complexes. IL-4 and IL-13 are N-glycoproteins. Glycan structures in glycoproteins play important roles in protein folding, protein stability, enzymatic function, inflammation, and cancer development. Therefore, the glycan structure of IL-4 and IL-13 needs to be elucidated in detail for the development of effective therapies. We report the first attempt to characterize the site-specific N-glycosylation of recombinant IL-4 and IL-13 via liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The tandem mass spectra of intact N-glycopeptides were identified using the Integrated GlycoProteome Analyzer (I-GPA) platform, which can automatically and rapidly analyze multiple N-glycopeptides, including their glycan composition and amino acid sequences. The recombinant IL-4 and IL-13 were identified with amino acid sequence coverages of 84% and 96%, respectively. For IL-4, 52 glycoforms on one N-glycosylation site were identified and quantified. In IL-13, 232 N-glycopeptides from three N-glycosylation sites were characterized, with the site Asn52 being the most extensively glycosylated (~80%). The complex glycans were the most abundant glycan on IL-4 and IL-13 (~96% and 91%, respectively), and the biantennary glycans were the most abundant in both recombinant IL-4 and IL-13 proteins.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.02a
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• pp.140-142
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• 2004

Since the April of 1978, Korea has strongly relied on the nuclear energy for electricity generation. As of today, eighteen nuclear power plants are in operation and ten are to be inaugurated by 2015. The installed nuclear capacity is 15, 716 MW as of the end of 2002, representing 29.3% of the nation's total installed capacity. The nuclear share in electricity remains around 38.9 at the end of 2002, reaching at the level of 119 billion kWh's. New power reactors, KSNP's (Korea Standard Nuclear Power Plant) are fully based on the domestic technologies. More advanced reactors such as KNGR (Korea Next Generation Reactor) will be commercialized soon. Even though the front end nuclear cycle enjoys one of the best positions in the world, there have been some chronical problems in the back end fuel cycle. That's the one of the reason why we need more active R&D programs in Korea and active international and regional cooperation in this area. The everlasting NIMBY problem hinders the implementation of the nation's radioactive waste management program. We expect that the storage capacity for the LILW(Low and Intermediate Level radioactive Waste) will be dried out soon. The situation for the spent fuel storage is also not so favorable too. The storage pools for spent fuel are being filled rapidly so that in 2008, some AR pools cannot accommodate any more new spent nuclear fuels. The Korean Government in strong association with utilities and national academic and R&D institutes have tried its best effort to secure the site for a LILW repository and a AFR site. Finally, one local community, Buan in Jeonbook Province, submitted the petition for the site. At the end of the last July, the Government announced that the Wido, a small island in Buan, is suitable for the national complex site. The special force team headed by Dr IS Chang, president of KAERI teamed with Government officials and many prominent scholars and journalists agreed that by the evidences from the preliminary site investigation, they could not find any reason for rejecting the local community's offer.

• Journal of Korean Society of Rural Planning
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• v.1 no.1
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• pp.49-64
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• 1995

As a basic research for the reorganization of the rural villages and the enhancement of the rural residential environment this study intends to compare attitudes and perceptions of the residents in the planned districts(Munhwa-Maul) and the existing districts in the two rural villages, Wolam-1-Ri and Woohang-2-Ri The major fadings of this study can be summarized as follows 1) The three types of reorganization of the rural villages are identified $.$ addition of the new residential site to existing village, development of a new village in a new site, redevelopment of existing village 2) The residents have low degrees of satisEaction at medical facilities, park and green spaces, public open spaces 3) The rates of residents who think their village territory include both the planned and the existing district is higher in the Woohang-2-Ri than in the Wolam-1-Ri 4) 57.6% of the farm householders in the two planned districts think the lot sizes are small, But the majority of the residents think the sizes of their houses are adequate. 5) A large number of residents show positive response to the development of the planned district 6) Since the majority of the residents(about 70%) in the two planned districts are not farm householders, new strategies need to be deveolped to induce more farm householders to the planned district.

• Spatial Information Research
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• v.8 no.2
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• pp.233-241
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• 2000

The objective of this study were to develop landscape scale ecosystem assessment model, and apply the model for the assessment of the state and change of ecosystem of the study area, Yongin, Korea. Since natural ecosystem of the site has been deteriorated significantly during recent extensive residential development, it is essential to correctly assess ecosystem of the study site. Traditional ecosystem assessment mainly utilizing intensive field survey requires high cost, but the outcome rarely represents spatial pattern of the regional ecosystems. Ecosystem assesment of landscape scale based on landscape ecology can resolve most of the shortfalls of the traditional approach. The research method can be summarized as follows. First, extensive literature review on such topics as spatial pattern of ecosystem, ecosystem assessment of landscape scale, ecological analysis was carried out. Second, a model for the ecosystem assessment of landscape scale emphasizing spatial pattern of ecosystem was developed. This model evaluates three indicators; ecological integrity and biological diversity, watershed integrity, and landscape resilience of 11 watersheds in the study area. Finally, ecological assessment utilizing two sets of indicators, enhancement of and disturbance of ecosystem stability, was carried out. This assessment method is based on Environmental Monitoring and Assessment Program´s Landscape component(EMAP-L) of EPA(1994). The results of this study are as follows. First, the ecosystem assessment of landscape scale of the study area of Yongin, Korea, showed that escosystems of Tanchun01 and Chungmichun01 watersheds had the worst state in the study site in 1991. On the other hand, the ecosystems of Jinwechun01, Kyunganchun02, and Bokhachun01 watersheds had the most stable ecosystem in 1991. Second, ecosystems of Tanchun01, Shingal reservoir, and Kyunganchun01 watersheds were evaluated to be the worst state in the study site in 1996. And, ecosystems of Jinwechun01 and Gosam reservoir watersheds had the most stable ecosystem. Third, ecosystem of Tanchun01 watershed which incudes Suji residential development project site changed the most drastically between 1991 and 1996. The ecosystem of the watershed the most drastically deteriorated due to it´s proximity to Seoul and Bundang new town.

• Journal of Ecology and Environment
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• v.35 no.2
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• pp.61-71
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• 2012

Recent conditions in North Sulawesi Province (NSP) have become favorable for the development of tourism. In this paper, we present the recent status of biodiversity and tourism in NSP as a basic consideration towards integrative biodiversity conservation strategy. Overall, biological accounts suggest that NSP is important for the world biodiversity conservation program. NSP's biodiversity makes the area a major nature-based tourism (ecotourism) site in the world. Development of diverse tourism programs in NSP has provided new opportunities for balancing development and conservation of regional ecosystems. However, the excessive tourism growth in some particular areas in NSP has been identified as the primary factor of environmental degradation. Nowadays, biodiversity of North Sulawesi regions are suffering from the number of tourist impacts and facilities. Based on those conditions, tourism planning and development in NSP is needed to formulate a proper strategy to protect the ecosystem and biodiversity from degradation and extinction. This will be a new challenge of sustainable island tourism development and biodiversity conservation in NSP.

• Korean Journal of Community Nutrition
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• v.15 no.1
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• pp.36-49
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• 2010

This study was carried out to reduce children's sugar, natrium and fat intakes and establish their healthy lifestyle. To achieve these goals, we developed an educational web site and an e-learning system. The targets of this web site are children, parents and educators. This site has various information about sugar, natrium and fat, and has special menus for each target: such as the 'Let's study' for children, 'Guides for child's eating' for parents, and 'Educational softwares, Lesson plans/materials, and Textbooks/Teaching guides' for educators. We developed three nutrient characters and applied them to the web site. We provided information in the form of texts, images, flash and sounds. This site has special boards in 'Nutrition cafe' menu to interchange information or their successful stories between the connecters. We developed an e-learning system with two courses. One is for junior elementary students and the other is for senior students. Children can study each nutrient step by step in a course according to their academic ability and concern. Also, they can evaluate their academic achievement in this system, which was uploaded into 'Let's study' in children menu in the web site. Conclusively this web site and e-learning system could contribute to reducing children's sugar, natrium and fat intakes by helping children study them systematically and effectively by on-line system. We expect this e-learning system would be a new nutrition education system to make nutrition education more active.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.04a
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• pp.458-463
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• 2000

Recently, there are increasing much concerns about repair and rehabilitation works for aged Concrete Structures which had been constructed on around the 1970's for rapid economic growth in Korea. In particular, it is believed that there are many aged concrete slabs for Highway bridges in these days. Thus new construction method of concrete slabs are strongly needed to minimize the traffic congestion during the repair works. The objective of this research is to develop the new constructional method of concrete slab in bridge structure, which can contribute to minimize the traffic congestion to be occurred during the repair and rehabilitation works of aged concrete slab, and can also assure the reliable quality through the minimization of in-situ works at the site. I-beams with punch holes will be manufactured in accordance with the specification in the factory, and will be preassembled into the panel. After erecting the preassembled panels in the site, concrete will be poured into the slab panel. This research is to investigate physical properties of I-Beam with punch holes itself, and then to investigate structural properties of assembled I-Beam panels through static and fatigue test, of which result can be utilized for the development of the new constructional method for concrete slab in bridge structure.

• Proceedings of the KSR Conference
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• 2009.05a
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• pp.1279-1307
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• 2009

The development of station districts is mainly categorized into 4 types. The first one is the composite development of the railroad station-areas. The second is the re-development of the direct station districts. The third is the rearrangement of the indirect station districts. And the fourth is the development of the new station districts for newly constructed railroad routes. Among these, this study aims to focus on the problems and methods of improvement in the development of the railroad station-areas, since there is not much problem with the implementation of the city development project, housing site development project or city rearrangement project for the direct or indirect station districts.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.730-738
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• 2016

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60℃ and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C₂-C₆). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.

• Journal of the Korean Institute of Rural Architecture
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• v.3 no.1
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• pp.11-23
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• 2001

It is focused on the desirable development alternations for sprawled unplanned on mountainous hilly areas in the residential areas development of the rural areas, mainly on the idyllic housing residential areas. The major research findings were as follows : (1) 85% of the idyllic housing residential areas of the suburban rural area was developed on the mountainous hilly areas. (2) For the desirable development on the suburban rural area, Environmentally Symbiosis Land Use Plan, diversification of Housing type such as Earthing Row House, diverse housing site planning techniques considering the conditions of mountainous hilly areas, for common space planning, land development, housing planing, hillside development, should be devised. (3) New regulations of strengthening construction permits, District Plan should be introduced in law and system management.