• 제목/요약/키워드: New Breeding Technique

검색결과 38건 처리시간 0.022초

New Strategy of Forest Tree Breeding for Society, Forest Science, and Forestry in Korea

  • Choi, Yong-Eui;Kim, Chul-Woo;Yi, Jae-Seon
    • Journal of Forest and Environmental Science
    • /
    • 제24권1호
    • /
    • pp.15-25
    • /
    • 2008
  • Social and scientific changes, i.e., global warming, desertification, pollution, biodiversity, bioenergy, plant variety protection, biotechnology, timber demand, reforestation in North Korea, and etc., were reviewed for new strategy of forest tree breeding in Korea. Diversified breeding goals, globalization of breeding target species, multidisciplinary research approaches, manpower networking, establishment of new administrative and research units in KFS and KFRI were proposed. Principles suggested for new tree breeding strategy are: 1) multi-disciplinary approach in settlement of objectives, breeding methods, and etc., 2) expansion of target trees including foreign species, 3) fulfillment of both domestic and international demands for forest tree breeding, 4) establishment of breeding program well-grounded on genetic resources conservation, 5) acknowledgement of breeding products (i.e., variety, technique, gene, and etc.) as goods, and 6) provision of more research opportunities for young scientists. Lastly, ongoing tree breeding project in Indonesia and NTFP R&D Center at the College of Forest and Environmental Sciences, Kangwon National University were introduced as examples of desirable breeding projects based on target species diversification, multidisciplinary approach, and manpower networking.

  • PDF

Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm

  • Ching-Fu Tu;Shu-Hui Peng;Chin-kai Chuang;Chi-Hong Wong;Tien-Shuh Yang
    • Animal Bioscience
    • /
    • 제36권2_spc호
    • /
    • pp.339-349
    • /
    • 2023
  • Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells, somatic cell nuclear transfer, cryopreservation, and nonsurgical embryo transfer. The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.

Development of a New Process for Mass-Production of Virus-Free Seed Bulbs of Garlic(Allium sativum L.) Through Plant Tissue Culture Technique

  • Kim, Joo-Hag
    • 한국생물환경조절학회:학술대회논문집
    • /
    • 한국생물환경조절학회 1996년도 국제심포지움 21세기 첨단식물생산시스템의 실용화
    • /
    • pp.117-128
    • /
    • 1996
  • A garlic (Allium sativum L.) is an important and useful vegetable such that it is not only most used for spices but also exploited for health foods and pharmaceuticals. The garlic is a kind of vegetable propagated vegetatively through cloves. Therefore, development of new varieties of garlics is possible only through selection breeding because cross breeding is impossible. (omitted)

  • PDF

Reducing CH4 Emissions from Grazing Ruminants in New Zealand: Challenges and Opportunities

  • Clark, H.;Kelliher, F.;Pinares-Patino, C.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제24권2호
    • /
    • pp.295-302
    • /
    • 2011
  • Almost half of New Zealand's greenhouse gas emissions arise from agriculture and enteric methane ($CH_4 $) emissions arising from ruminant animals constitute 30% of total $CO_2$-e emissions. Enteric $CH_4$ emissions have increased by 9% since 1990. Extensive research has been undertaken to develop reliable methods for measuring enteric $CH_4$ emissions. New Zealand studies using the SF6 tracer technique suggest that on average this technique yields similar values to the 'gold' standard of calorimetry, but with a larger variance. National inventory estimates based on results obtained using the $SF_6$ technique will therefore overestimate the uncertainty. Mitigating emissions can be achieved by changing feed type but there are practical and cost barriers to the use of alternative feeds. Forages containing condensed tannins do reduce emissions but are agronomically inferior to the forages currently used. Rumen additives have shown some success in-vitro but results from in-vivo trials with both monensin and fumaric acid have been disappointing. The development of methods for directly manipulating rumen microorganisms are at an early stage and work to develop vaccines that can inhibit methanogenesis has yielded mixed results. The successful identification of sheep with contrasting $CH_4$ yields raises the possibility that, in the long term, a breeding approach to $CH_4$ mitigation is feasible.

Perspectives on high throughput phenotyping in developing countries

  • Chung, Yong Suk;Kim, Ki-Seung;Kim, Changsoo
    • 농업과학연구
    • /
    • 제45권3호
    • /
    • pp.317-323
    • /
    • 2018
  • The demand for crop production is increasingly becoming steeper due to the rapid population growth. As a result, breeding cycles should be faster than ever before. However, the current breeding methods cannot meet this requirement because traditional phenotyping methods lag far behind even though genotyping methods have been drastically developed with the advent of next-generation sequencing technology over a short period of time. Consequently, phenotyping has become a bottleneck in large-scale genomics-based plant breeding studies. Recently, however, phenomics, a new discipline involving the characterization of a full set of phenotypes in a given species, has emerged as an alternative technology to come up with exponentially increasing genomic data in plant breeding programs. There are many advantages for using new technologies in phenomics. Yet, the necessity of diverse man power and huge funding for cutting-edge equipment prevent many researchers who are interested in this area from adopting this new technique in their research programs. Currently, only a limited number of groups mostly in developed countries have initiated phenomic studies using high throughput methods. In this short article, we describe the strategies to compete with those advanced groups using limited resources in developing countries, followed by a brief introduction of high throughput phenotyping.

DNA 지문을 이용한 가금의 유전분석과 개량 (DNA Fingerprinting in Poultry Breeding and Genetic Analysis)

  • 여정수
    • 한국가금학회지
    • /
    • 제22권2호
    • /
    • pp.97-104
    • /
    • 1995
  • Recently, DNA fingerprinting has been utilized as the most powerful tool for genetic analysis and improvement of poultry. This technique enables us to solve several problems of poultry breeding ; traits of low heritability, difficulty in keeping the performance records, measuring in late of life, and sex limited traits. Application of DNA fingerprinting is chiefly focused to individual and population identification, evolution force, quantitative trait marker, introgression of new gene, and prediction of heterosis. Thus, research work on DNA fingerprinting will he accelerated to analyze genetic components exactly and improve the performance of poultry.

  • PDF

육종과 생명공학 (Plant Breeding and Biotechnology)

  • 박효근
    • 한국식물학회:학술대회논문집
    • /
    • 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
    • /
    • pp.9-18
    • /
    • 1987
  • The plant breeding, a discipline of agricultural sciences, has greatly contributed to huan welfare in relieving food crisis by development of higher yielding, stronger resistant and better quality varieties. However, many conventional plant breeders, especially ones working for major crops, are facing exhaustion of useful genetic variability, which greatly limit the potentional of developing better cultivars. Therefore, the convectional plant breeders have been eagerly looking for new renovational methods in creating genetic varibility. It has been expected that biotechnology would provide the technique to create totally new genetic variability through gene transfer, chromosome manipulation and/or cell fusion. It is strongly suggested that very close interdisciplinary approaches between convectionla plant breeders and biotechnoligists is essentional for opening new era in developing better varieties.

  • PDF

식량생산능력 향상을 위한 농작물 육종전략 (Breeding Strategies to Increase Production Potential of Major Food Crops in Korea)

  • 김광호;김석동;박문웅;문헌팔
    • 한국작물학회:학술대회논문집
    • /
    • 한국작물학회 1999년도 추계 학술대회지
    • /
    • pp.80-101
    • /
    • 1999
  • Self-sufficiency ratio of food crops in Korea is estimated under $20{\%}$ in 2010 because total food consumption including feed will be increased. but food grain production will be decreased. It is necessary to maintain the optimum level of food self-sufficiency rate to secure national food demand/supply balance and non-trade and multiple function of agriculture in Korea. It will be possible to produce more food grains having the acceptable quality if the appropriate policy and cropping techniques are developed and practised in future. Breeding for high yielding varieties should be the first target to raise the production potential of food crops . Number of varieties developed during last 30 years is counted as 353 in food crops. New varieties developed in 1990s showed the higher yield potential and the improved agronomic characteristics compared with 1970s and 1980s varieties. But number of varieties planted on the farmer's field over $5{\%}$ of national planting area is less than one third of total varieties developed Breeding efforts to maintain planting area of main food crops should be focussed on consumer's demand and farmer's need. They are the best quality variety in each field of crop utilization, the newly designed variety adapting to changes of natural, rural and cropping environment, and the higher yielding variety. It is also needed to develop new quality crop varieties for inducing more consumption of crop grain produced in Korea for direct food or processing. Development of barley varieties for animal feed. high income soybean varieties, high quality wheat variety. and super yielding rice and barley varieties are also needed to keep or maximize national food production potential. In order to establish the appropriate cropping technique for domestic food security, the strong and continuous interest and financial support on crop breeding are required, and the inter-disciplinary and inter-institutionary researches should be strengthened for successful crop breeding.

  • PDF

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

  • Xu, R.F.;Li, K.;Chen, G.H.;Qiang, B.Y.Z.;Mo, D.L.;Fan, B.;Li, C.C.;Yu, M.;Zhu, M.J.;Xiong, T.A.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제18권7호
    • /
    • pp.942-948
    • /
    • 2005
  • New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

유채 품질연구 현황과 문제점 및 방향 (Status and Prospects of Seed Quality in Researches in Rapeseed)

  • 이정일;방진기;권병선;강광희
    • 한국작물학회지
    • /
    • 제33권s01호
    • /
    • pp.98-114
    • /
    • 1988
  • This study was conducted to investigate the status and prospects of seed quality researches in rapeseed. Rapeseed Quality was mainly related to oil and protein content, fatty acid composition and glucosinolate content. Hence, breeding for improvement of rapeseed Quality has been emphasized as follows: 1) inheritance mode, 2) investigation of germplasm, 3) establishment of analysis technique, 4) establishment of selection method, 5) idealization of cultural technique. The oil quality is determined by its fatty acids. Fatty acids have been determined by gas chromatography. To improve oil quality was emphasized for zero erucic acid, the highest possible linoleic acid and the lowest possible linolenic acid content. Rapeseed meal is not considered as top quality feed ingredient although it has higher protein content and well-balanced amino acid composition. This is mainly because of the presence of considerable amounts of glucosinolates. Thus the reduction of glucosinolate content in rapeseed meal is of great importance. In breeding for meal quality, low glucosinolate lines (plants) were selected and analyzed by gas chromatography and UV-spectrophotometer. Current problems and future researches of rapeseed quality in Korea are 1) improvement of researcher's number and facilities, 2) depression of animal feeding trials, 3) unsatisfied relationship between research and manufacturing and products field, 4) improvement of fertility for yellow and thin seed coat lines crossed between mustard and rapeseed, 5) establishment of new rapid analysing system for rapeseed quality.

  • PDF