• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
• /
• pp.103.2-103.2
• /
• 2003

The hydrolysis of sphingomyelin (SM), known as the SM pathway, is induced by the activation of sphingomyelinase (SMase) to generate the second messenger ceramide, which plays a key role in cellular responses such as apoptosis, differentiation, senescence, and inflammation. Here, we identified a 60 kDa membrane-associated, neutral and Mg$\^$2+/-dependent SMase, termed N-SMase $\varepsilon$, from mammalian brains, which was revealed as the heat shock protein 60 (HSP60) through cDNA cloning and mass spectrometrical analysis. (omitted)

• Ocean and Polar Research
• /
• 제34권2호
• /
• pp.151-163
• /
• 2012

이매패류의 소화맹낭은 고도로 발달된 리소솜 시스템을 지니고 있어 소화작용과 함께 오염물질의 해독과 배출작용을 담당한다. 따라서 소화맹낭의 리소솜 시스템은 환경변화에 민감하게 반응한다고 알려져 있다. 이 연구에서는 동결절편을 이용한 조직학적 방법으로 조간대에 서식하는 참굴 소화맹낭의 리소솜 활동을 측정하였다. 참굴은 2010년 6월 광양만 내만의 초남대교, 차면리와 외만의 신덕리, 평산리 조간대에 서식하는 굴을 채집하였다. 제작된 동결절편을 이용하여 lysosomal membrane stability(LMS) 및 lipofuscin(LF) 축적, neutral lipid(NL) 축적, 소화맹낭 위축도(digestive gland atrophy, DGA)를 평가하였다. 비만도 및 소화맹낭 위축도는 내만에 서식하는 참굴이 다른 지역에 비해 유의적으로 낮았다(P<0.05). LMS는 내만이 외만에 비해 짧은 리소솜 막 불안정화 시간을 보였지만 지역별 통계적인 유의차는 없었다. LF 축적은 내만에 서식하는 참굴이 유의적으로 높은 축적을 보였지만(P<0.05), NL 축적의 경우 외만에 서식하는 참굴이 유의적으로 높은 축적을 보였다(P<0.05). DGA와 LF의 경우 내만과 외만에서 뚜렷한 차이를 보였고, 이는 환경오염의 수준과 관련 있을 것으로 사료된다. 따라서 이 두 가지 분석항목은 연안환경평가에서 좋은 biomarker로 사용될 수 있을 것으로 기대된다.

• 한국의류학회지
• /
• 제40권6호
• /
• pp.1034-1044
• /
• 2016

This study seeks a method to objectively evaluate sensibility based on Big Data in the field of design. In order to do so, this study examined the sensibility responses on design factors for the public through a network analysis of texts displayed in social media. 'Hanbok', a formal clothing that represents Korea, was selected as the subject for the research methodology. We then collected 47,677 keywords related to Hanbok from 12,000 posts on Naver blogs from January $1^{st}$ to December $31^{st}$ 2015 and that analyzed using social matrix (a Big Data analysis software) rather than using previous survey methods. We also derived 56 key-words related to design elements and sensibility responses of Hanbok. Centrality analysis and CONCOR analysis were conducted using Ucinet6. The visualization of the network text analysis allowed the categorization of the main design factors of Hanbok with evaluation terms that mean positive, negative, and neutral sensibility responses. We also derived key evaluation factors for Hanbok as fitting, rationality, trend, and uniqueness. The evaluation terms extracted based on natural language processing technologies of atypical data have validity as a scale for evaluation and are expected to be suitable for utilization in an index for sensibility evaluation that supplements the limits of previous surveys and statistical analysis methods. The network text analysis method used in this study provides new guidelines for the use of Big Data involving sensibility evaluation methods in the field of design.

• 정보관리학회지
• /
• 제28권3호
• /
• pp.335-353
• /
• 2011

본 연구에서는 리커트 5점 척도 설문에 참여했던 응답자들이 특정 자극에 의해 동일한 2차 설문이 주어졌을 때, 본인의 처음 응답결과를 어떻게, 어떤 방향으로 바꾸는지를 측정하고자 하였다. 이를 위해 3개 설문지의 1차, 2차 또는 3차에 걸친 재설문 결과들을 집단간 차이, 자극의 종류, 설문목적의 차이, 응답변화의 방향 및 변화정도의 관점에서 분석하였다. 본 연구를 통해 발견된 연구결과는 다음과 같다. 첫째, 전문가 집단과 일반 집단 중 전문가 집단이 상대적으로 의견을 바꾸지 않는 것으로 유의하게 검증되었다. 둘째, 설문목적에 따른 차이는 없는 것으로 조사되었고, 자극의 종류 중 시스템 사용경험보다는 다른 사람들의 의견이 정보로 주어졌을 때 더 많은 영향을 받는 것으로 나타났다. 셋째, 최초응답에서 긍정적인 답변에 응답했을수록 답변을 바꾸지 않는 것으로 나타났고 부정적인 답변자는 대부분 긍정적인 방향으로 의견을 바꾸는 것으로 유의하게 검증되었다. 넷째, 최초 응답을 바꾸는 경우 응답변화의 폭이 2단계 이상인 경우는 그다지 많지 않고 대부분 1단계 정도 전후로 바꾸는 경향이 있는 것으로 분석되었다. 마지막으로 중간응답자가 의견을 가장 많이 바꾸고 양극단의 경우 의견을 잘 바꾸지 않을 것이라는 예측을 하였으나 부정적인 답변자일수록 의견을 긍정적인 방향으로 바꾸는 것으로 분석되었다.

• 한국토양비료학회지
• /
• 제27권2호
• /
• pp.136-146
• /
• 1994

대두 근류균 공생에서 뿌리분비물에 대한 근류균의 화학주성이 근류형성에 미치는 영향을 조사 연구한 결과는 다음과 같다. 2mM proline에 대한 표준균주와 변이주의 화학주성비는 KCTC 2422는 3.1, LPN-100은 1.3, LCR-101은 1.0이었으며 전형적인 Slow growing Rhizobia였다. 팔달 및 백운의 뿌리분비물 추출액에 대한 근류균의 화학주성비는 KCTC 2422는 3.5/3.2 이고 LPN-100은 1.3/1.4이며 LCR-101은 1.4/1.4정도였다. Dowex 칼럼으로부터 용출시킨 Fraction I, II, III에 대한 화학주성비는 Fraction I>II>III순이었고 중성분획인 Glucose와 Ribose, 양성분획인 Aspartic acid와 Glutamine, 음성분획인 Citric acid, Succinic acid에 대한 화학주성비는 공히 $10^{-3}M$일때 가장높은 화학주성비를 나타내었다. 근류형성의 정도는 KCTC 2422는 접종후 7일경부터 Nitrogenase 활성이 나타나기 시작하며 LCR-101은 15일 이후에 Nitrogenase 활성이 나타났으며 LPN-100은 전혀 근류를 형성하지 않아 근류균의 화학주성이 숙주인식의 초기과정에 영향을 미쳤음을 확인할 수 있었다.

• Applied Microscopy
• /
• 제28권3호
• /
• pp.329-343
• /
• 1998

Early wound healing responses in the skin of the Korean fire bellied toad, Bombina orientalis, were examined using histochemical , scanning and transmission electron microscopical techniques. Cutaneous wounds were induced by sharp razor blade , and maintained in special cages for up to 3 days. By this treatment whole parts of epithelium and dermis were profoundly affected. After injury, retraction of damaged surface of the skin was observed. At 3 hours after injury, poly-band was form by mucous substance. The wound skin consisted of mucous gland secreted mucous substance which flows into wound cavity and the wound area was sealed by mucous substance which form thick layer at 6 hours. It appeared that natural disposition showed acid and neutral mucous substance by reaction of PAS and Alcian blue (pH 2.5) staining. After formation of the poly-band, epithelial cells were formed by migration of regenerating epithelial cells. These cells moved over the wound surface by migration at 12 hours after wounding. Poly-band was much increased in thickness and migrating cells were completely formed wound closure at 24 hours post-wounding. After 72 hours regenerated epithelial layer consists of four or five cell layers which are similar to clear, granular, and spinous layer.

• 한국감성과학회:학술대회논문집
• /
• 한국감성과학회 1997년도 한국감성과학회 연차학술대회논문집
• /
• pp.228-232
• /
• 1997

The special interest should be paid to the analysis of the influences of positive emotions in terms of their possible effects on the dyanmics of autonomic. recovery after the negative affective stimualtion. Taking into account emotion-specific autonomic response patterning and dissociation of parameters of autonomic arousal during experience of both positive and negative emotional states, this problem seems a challenging one. In present study several autonomic parameters were analyzed altogether, namely inedices of electrodermal activity, heart rate and respitation rate during consecutive combination or both IAPS-based visual affective and auditory stimulation. The aim of the study was analysis of patterns of electrodermal and cardiorespiratory responses during emotional states evoked by negative affective visual stimulation followed by positive or neutral auditory one with intention to identify if the latter is able to facilitate post-stress recovery and enhance restoration of pre-arousal levels. The main orientation was dirdcted towards the further application of experimentally induced comfort emotions for dampening the negative consequences of exposure to stressful stimuli.

• 한국프랜차이즈경영연구
• /
• 제14권4호
• /
• pp.1-16
• /
• 2023

Purpose: Previous studies show that perceived CSR motives have a significant impact on company evaluations. However, consumer responses to CSR motives vary depending on CSR motives. From this perspective, this study investigates the impact of CSR motives on consumers' responses in the context of food and beverage franchise companies using a scenario. Research design, data, and methodology: For achieving the purposes of the study, an example of a domestic food and beverage franchise company actively carrying out CSR activities was presented. Data was collected from 304 respondents aged 20 or older who were aware of CSR activities. The respondents answered the questionnaire after reading the scenario. The data was analyzed with SPSS 28.0 and SmartPLS 4.0 program. Result: Values-driven motive and strategic motive influence authenticity, while stakeholder-driven motive and egoistic motive did not influence authenticity. Values-driven motive influences on attitude, while stakeholder-driven motive, strategic motive and egoistic motive didn't. Lastly, authenticity influences attitude. Conclusions: Companies need to be aware that consumers may infer different motives for their CSR activities, and pay close attention to consumers' perceived motives from the planning stage of CSR activities. In particular, companies should focus on the values-driven motive and the strategic motive when planning CSR activities.

• The Korean Journal of Physiology
• /
• 제24권1호
• /
• pp.171-180
• /
• 1990

In 19 cats anesthetized with ${\alpha}-chloralose$ effects of taurine and ${\beta}-alanine$ on the responses of wide dynamic range (WDR) cells to mechanical, chemical and thermal stimuli were investigated in the lumbar spinal cord of the cat. Also studied was an interaction of strychnine with taurine in affecting the activities of WDR cells. Following intravenous administration of taurine, the responses of WDR cells to all types of mechanical stimuli were markedly enhanced, demonstrating that the response to pressure was most sensitive to taurine action. When the receptive field was exposed to thermal stimuli ($50^{\circ}C$) for 20 sec. taurine increased activity of WDR cell to 169.5% of the control value. The $K^{+}$-induced activation of WDR cells was invariably suppressed after taurine administration. Intravenously administered strychnine remarkably reduced the enhanced response of WDR cell to natural stimuli resulting from intravenous administration of taurine. Also ${\beta}-alanine$ markedly activated the response of spinal dorsal horn cell to natural mechanical stimuli. These findings suggest that neutral amino acid and its derivative such as ${\beta}-alanine$ and taurine can enhance the response of WDR cells to different stimuli in cats.

• 한국연초학회지
• /
• 제32권1호
• /
• pp.19-27
• /
• 2010

The aim of this study is to compare the sensitivity of both two NCI-H292 and A549 cell types to acute inflammatory responses induced by cigarette smoke. For this, we treated two kinds of smoke fractions derived from 2R4F reference cigarettes: total particulate matter(TPM) collected onto a Cambridge filter pad and gas/vapor phase(GVP) prepared by bubbling through in buffer solution. When we measured cellular cytotoxicity by neutral red uptake assay after treatment for 24 hours, TPM and GVP induced cytotoxic effect in a dose-dependent manner in the range of 10-$100{\mu}g$/mL and 60-$300 {\mu}g$/mL., respectively, in both cell types without any cellular difference. Additionally, when we examined acute inflammatory responses by analyzing cytokines secreted into culture media including tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-8(IL-8), and transforming growth factor-$\alpha$(TGF-$\alpha$) as well as matrix metalloproteinase-1(MMP-1), the treatment with smoke fractions increased those marker proteins in a dose-dependent manner in NCI-H292. Meanwhile, in A549 cells only MMP-1 was observed to be increased in a dose-dependent fashion. Collectively, our data indicate that NCI-H292 cell type is more sensitive to cigarette smoke-induced inflammatory response than A549 cells. This suggests that NCI-H292 could be useful as an in vitro evaluation tool to assess harmful effects of cigarette smoke.