• Bulletin of the Korean Chemical Society
• /
• 제31권11호
• /
• pp.3173-3179
• /
• 2010

New complex $[Mn(II)H_{1.5}L]_2[Mn(II)H_3L]_2(ClO_4)_5{\cdot}3H_2O$ (1), where $H_3L$ is tris {2-(4-imidazolyl)methyliminoethyl} amine (imtren), has been prepared by reacting manganese(II) perchlorate hexahydrate with the imtren ligand in methanol. X-ray crystallographic study revealed that the imtren ligand hexadentately binds to Mn(II) ion through the three Schiff-base imine N atoms and three imidazole N atoms with a distorted octahedral geometry, and the apical tertiary amine N atom of the ligand pseudo-coordinates to Mn(II), forming overall a pseudo-seven coordination environment. The hydrogen-bonds between imidazole and imidazolate of $[Mn(II)H_{1.5}L]^{0.5+}$ complex ions are extended to build a 2D puckered network with trigonal voids. $[Mn(II)H_3L]^{2+}$ complex ions constitutes another extended 2D puckered layer without hydrogen bonds. Two layers are wedged each other to constitute overall stack of the crystal. Peroxidase activity of complex 1 was examined by observing the oxidation of 2,2'-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) with hydrogen peroxide in the presence of complex 1. Generation of $ABTS^{+{\cdot}}$ was observed by UV-vis and EPR spectroscopies, indicating that the complex 1, a fully-coordinated mononuclear Mn(II) complex with nitrogen-only ligand, has a heme-independent peroxidase activity.

• Bulletin of the Korean Chemical Society
• /
• 제35권1호
• /
• pp.218-224
• /
• 2014

In order to explore new coordination frameworks with novel designed 3-nitrophthalic acid and the same N-donor ancillary ligand, a series of novel coordination complexes, namely, $[Cd_2(3-NPA)_2(TBZ)_2(H_2O)_2]{\cdot}2H_2O$(1), $[Zn_2(3-NPA)_2(TBZ)_2]$(2), $[Zn_2O(3-NPA)(TBZ)(H_2O)]_n$(3), $[Co(3-NPA)(TBZ)(H_2O)]_n$(4) (3-$NPAH_2$ = 3-nitrophthalic acid), have been hydrothermally synthesized through the reaction of 3-nitrophthalic acid with divalent transition-metal salts in the presence of N-donor ancillary coligand (TBZ = thiabendazole). As a result of various coordination modes of the versatile 3-$NPAH_2$ and the coligand TBZ, these complexes exhibit structural diversity. X-ray structure analysis reveals that 1 and 2 are 0D molecular rings, while 3 and 4 are one-dimensional (1D) infinite chain polymers. And the weak O-H${\cdots}$O hydrogen bonds and C-H${\cdots}$O nonclassical hydrogen bonds as well as ${\pi}-{\pi}$ stacking also play important roles in affecting the final structure where complexes 1, 3 and 4 have 3D supramolecular architectures, while complex 2 has a 2D supramolecular network. Also, IR spectra, fluorescence properties and thermal decomposition process of complexes 1-4 were investigated.

• 한국결정학회지
• /
• 제15권2호
• /
• pp.78-82
• /
• 2004

벤젠 존재 하에서, DMF와 에탄올의 혼합 용매에서 zinc(II) nitrate $(Zn(NO_3)_2\;{\cdot}\;6H_2O)$와 $ATP(2-aminoterephthalate,\;H_2N-C_6H_3-1,4-(COO)_2)$의 용매열 반응으로 2차원 배위 고분자 [Zn(ATP) (DMF)] (1)이 얻어졌다 X-ray구조 결정 결과, 2개의 아연 금속과 4개의 ATP 리간드가 paddle-wheel유형의 2차 쌓음 단위들을 형성하고, 이것들은 ATP 리간드에 의해서 연결되어 2차원 4각 망을 이룬다는 것이 밝혀졌다. 아연 금속을 기준으로 각 4각형의 크기는 약$11.1\times11.1\;{\AA}$효이다. 고분자 1을 양질의 결정 상태로 얻기 위해서는 벤젠이 요구되었다.

• IMMUNE NETWORK
• /
• 제11권1호
• /
• pp.50-58
• /
• 2011

Background: Epstein-Barr virus associated gastric lymphoepithelioma-like carcinoma (LELC) is characterized by the intensive infiltration of lymphoid cells, the presence of EBV, and the better prognosis over typical adenocarcinoma. Thus, it was assumable that viral latent proteins may be responsible for the recruitment of a certain T cell repertoire to EBV-associated gastric carcinoma. Methods: To examine above possibility, EBV gene expression in gastric carcinoma tissues and usage of TCR among the tumor infiltrating lymphocytes were analyzed. Results: EBV specific DNA and EBERs RNA were detected in 4 out of 30 patients. RT-PCR analysis revealed that all 4 of EBV-positive tumor tissues expressed EBNA1 mRNA and BARTs and LMP2a was detected only one sample out of 4. However, the EBNA2 and LMP-1 transcripts were not detected in these tissues. $CD8^+$ T cells were the predominant population of infiltrating lymphocytes in the EBV-positive gastric carcinoma. According to spectra type analysis of infiltrating T cells, 10 predominant bands were detected by TCR $V{\beta}$ CDR3 specific RT-PCR from 4 EBV-positive tumor tissues. Sequence analysis of these bands revealed oligoclonal expansion of T cells. Conclusion: These findings suggest that clonally expanded T cells in vivo might be a population of cytotoxic T cells reactive to EBV-associated gastric carcinoma.

• Bulletin of the Korean Chemical Society
• /
• 제31권6호
• /
• pp.1583-1588
• /
• 2010

A single step fabrication process of carbon nanotube/Prussian Blue (CNT/PB) paste electrodes based on screen printing technology has been studied as an amperometric sensor for the determination of hydrogen peroxide and free chlorine. Compared to the classical carbon paste (CP) electrode, the CNT paste electrode greatly enhanced the response in the presence of hydrogen peroxide due to the electrocatalytic activity of the CNT. Based on the CNT/binder paste, PB was also incorporated into a network of CNT paste and characterized. The best electroanalytical properties of PB-mixed sensors to hydrogen peroxide were obtained with PB ratio of 10 wt % composition, which showed fast response time ($t_{90}{\leq}5$ s; 0.2 - 0.3 mM), low detection limit of 1.0 ${\mu}M$, good linear response in the range from $5.0{\times}10^{-5}$ - $1.0{\times}10^{-3}$ mol $L^{-1}$ ($r^2$ = 0.9998), and high sensitivity of -8.21 ${\mu}AmM^{-1}$. In order to confirm the enhanced electrochemical properties of CNT/PB electrode, the sensor was further applied for the determination of chlorine in water, which exhibited a linear response behavior in the range of 50 - 2000 ppb for chlorine with a slope of 1.10 ${\mu}Appm^{-1}$ ($r^2$ = 9971).

• 한국지능시스템학회논문지
• /
• 제21권1호
• /
• pp.12-18
• /
• 2011

본 논문에서는 RBF 뉴럴 네트워크에서 은닉층 활성함수에 Interval type-2 퍼지개념을 적용한 새로운 RBF 뉴럴 네트워크를 설계하였다. 퍼지 시스템 분야에서 불확실한 정보에 대한 Type-1 퍼지집합의 성능을 보안하고자 Type-2 퍼지집합이 제안되었으며, 멤버쉽함수 안에 다시 멤버쉽함수를 생성함으로써 불확실한 정보를 좀 더 효과적으로 다루고자 하였다. 따라서 본 논문에서는 RBF 뉴럴 네트워크의 은닉층 활성함수에 type-2 퍼지집합의 개념을 적용하여 불확실한 정보에 대한 모델 성능을 개선하고자 하였다. 나아가 연결가중치를 상수항이 아닌 1차식으로 구성된 다항식을 사용하여 최종출력을 입력-출력의 관계식으로 표현하였다. 연결가중치는 기존의 경사하강법(Gradient Descent Method; GDM) 대신 conjugate gradient method(CGM)을 사용하여 파라미터를 동조하고, 은닉층의 활성함수는 공간탐색 진화 알고리즘(Space Search Evolutionary Algorithm; SSEA)을 이용하여 가우시안 함수의 중심점 및 분포상수를 동조하여 모델의 성능을 개선시킨다. 제안된 모델의 성능을 평가하기 위해 가스로 시계열 데이터를 사용하였으며, 결과를 기존 모델과 비교하였다.

• Smart Structures and Systems
• /
• 제6권7호
• /
• pp.835-849
• /
• 2010

This study investigated propagation characteristics of piezo-activated guided waves in an aluminium rectangular-section tube for the purpose of damage identification. Changes in propagating velocity and amplitude of the first wave packet in acquired signals were observed in the frequency range from 50 to 250 kHz. The difference in guided wave propagation between rectangular- and circular-section tubes was examined using finite element simulation, demonstrating a great challenge in interpretation of guided wave signals in rectangular-section tubes. An active sensor network, consisting of nine PZT elements bonded on different surfaces of the tube, was configured to collect the wave signals scattered from through-thickness holes of different diameters. It was found that guided waves were capable of propagating across the sharp tube curvatures while retaining sensitivity to damage, even that not located on the surfaces where actuators/sensors were attached. Signal correlation between the intact and damaged structures was evaluated with the assistance of a concept of digital damage fingerprints (DDFs). The probability of the presence of damage on the unfolded tube surface was thus obtained, by which means the position of damage was identified with good accuracy.

• Journal of Ginseng Research
• /
• 제44권5호
• /
• pp.690-696
• /
• 2020

Background: As the main metabolites of ginsenosides, 20(S, R)-protopanaxadiol [PPD(S, R)] and 20(S, R)-protopanaxatriol [PPT(S, R)] are the structural basis response to a series of pharmacological effects of their parent components. Although the estrogenicity of several ginsenosides has been confirmed, however, the underlying mechanisms of their estrogenic effects are still largely unclear. In this work, PPD(S, R) and PPT(S, R) were assessed for their ability to bind and activate human estrogen receptor α (hERα) by a combination of in vitro and in silico analysis. Methods: The recombinant hERα ligand-binding domain (hERα-LBD) was expressed in E. coli strain. The direct binding interactions of ginsenosides with hERα-LBD and their ERα agonistic potency were investigated by fluorescence polarization and reporter gene assays, respectively. Then, molecular dynamics simulations were carried out to simulate the binding modes between ginsenosides and hERα-LBD to reveal the structural basis for their agonist activities toward receptor. Results: Fluorescence polarization assay revealed that PPD(S, R) and PPT(S, R) could bind to hERα-LBD with moderate affinities. In the dual luciferase reporter assay using transiently transfected MCF-7 cells, PPD(S, R) and PPT(S, R) acted as agonists of hERα. Molecular docking results showed that these ginsenosides adopted an agonist conformation in the flexible hydrophobic ligand-binding pocket. The stereostructure of C-20 hydroxyl group and the presence of C-6 hydroxyl group exerted significant influence on the hydrogen bond network and steric hindrance, respectively. Conclusion: This work may provide insight into the chemical and pharmacological screening of novel therapeutic agents from ginsenosides.

• IMMUNE NETWORK
• /
• 제7권3호
• /
• pp.117-123
• /
• 2007

Background: This study was designed to investigate the role of the hepatocyte growth factor (HGF) with regards to differentiation of somatic stem cells originating from the human umbilical cord blood (UCB) into hepatic lineage cells in vitro culture system. Methods: Mononuclear cells from UCB were cultured with and without HGF based on the fibroblast growth factor (FGF)-1, FGF-2, and stem cell factor. The cultured cells were confirmed by immunofluorescent staining analysis with albumin (ALB), cytokeratin-19 (CK-19), and proliferating cell nuclear antigen (PCNA) MoAb. ALB and CK-18 mRNA were also evaluated by reverse transcription-polymerase chain reaction. In order to observe changes in proliferating capacity with respect to the cultured period, CFSE with affinity to proliferating cells were tagged and later underwent flow cytometry. Results: In the HGF-treated group, cultured cells had a large oval shaped appearance with adherent, but easily detachable characteristics. In the HGF-non treated group, these cells were spindle-shaped with strong adherent characteristics. Expressions of ALB and CK-19 were evident in HGF-treated group compared to non-expression of those in to HGF-non treated group. Dual immunostaining analysis of the ALB producing cells showed presence of PCNA in their nuclei, and ALB and CK-18 mRNA were detected on the 21st day of cultured cells in the HGF-treated group. Conclusion: Our findings suggest that HGF has a pivotal role in differentiating somatic stem cells of human UCB into hepatic lineage cells in vitro.

• 디지털콘텐츠학회 논문지
• /
• 제8권4호
• /
• pp.491-497
• /
• 2007

무선 홈 응용을 실현하는데 있어서 근거리 네트워킹 기술의 중요성은 증가하고 있다. 예를 들어, 블루투스와 IEEE 802.11b 표준은 무선 홈 응용 구축을 위한 가장 일반적으로 사용되고 있는 기술들이다. 하지만 두 표준은 동일한 무허가 ISM(Industrial, Scientific, Medical) 무선 스펙트럼 대역을 공유하기 때문에 심각한 간섭을 피할 수 없으며, 두 기술을 사용하는 이종 디바이스가 아주 근접하여 있을 경우에 시스템 성능은 심각하게 손상을 받는다. 본 논문에서 연구한 가우시안 FSK 블루투스 시스템은 전자기기 사이의 무선 연결성을 위한 근거리 무선통신 기술의 표준이다. 본 논문에서는 가우시안 FSK 블루투스 시스템에서 동일채널간섭의 영향을 분석하였다. 본 연구에서 사용한 성능 평가 요소는 무선 채널에서의 SIR, 간섭 지수 $\rho$, 그리고 BER 등이며 이를 기반으로 성능을 평가하였다. 가우시안 FSK 블루투스 시스템의 성능 평가에서 다양한 소스로 부터의 동일채널간섭의 영향은 IGA(Impulsive Gaussian Approximation) 방식을 이용하여 분석하였고 다양한 채널상태를 나타내기 위하여 Eb/No, $\rho$와 SIR 값에 따라서 블루투스 시스템의 성능을 분석하여 결과 그래프에 나타내었다.