• Journal of the Optical Society of Korea
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• 제7권4호
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• pp.245-248
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• 2003

Propagation of light along a pericardium meridian is measured and compared with that along a non-meridian path one cm away from the meridian. Source-light is illuminated on the Nei-Guan(PC6) and propagated light is detected at three points along the meridian. Four reference points are one cm away from the corresponding meridian points, respectively. Light propagates better along the meridian than the reference path with more than twenty percent difference for all the subjects tested.

• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1188-1191
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• 2004

Two species of marine catfish, Arius maculatus and Osteogeneiosus militaris, belonging to family Ariidae were analysed electrophoretically for genetic variation in 6 enzymes, alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), lactate dehydrogenase (LDH), glucose dehydrogenase (GDH), malic enzyme (ME) and superoxide dismutase (SOD). Eighteen individuals of each species were studied. Two loci MDH and ADH were polymorphic in both. Average heterozygosity in A. maculatus was 1.46, while it was 2.5 in O. militaris. The allele frequencies were used to estimate Nei's genetic distance (D). The D value was calculated to be 0.6879. Two isozyme loci, ME and SOD, were found to be the most reliable species specific markers. No tissue specific loci were observed for the enzymes studied, the bands being identical in each case. The genetic distance observed between O. militaris and A. maculatus in this study suggests that they would be more appropriately classified as species of the same genus rather than being assigned separate genera.

• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1696-1700
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• 2005

Five China native cattle breeds have been characterized by using 10 microsatellite DNA markers. The studied populations can be divided into five groups: Luxi cattle, Nanyang cattle, Jinnan cattle, Qinchuan cattle and Yanbian cattle. Allele frequencies were calculated and used for the characterization of the breeds and the study of their genetic relationships. Heterozygosity, polymorphism information content, the effective number of alleles was calculated. Nei' standard genetic distance (1978) was calculated and used for a neighbor-joining tree construction. NJ tree showed that Luxi cattle, Nanyang cattle, Jinnan cattle and Qinchuan cattle are closely related, whereas Yanbian cattle are clearly distinct from other four populations. The genetic relationship of five breeds corresponds to their history and geographic origins. This work analyzes the recent origin of these populations and contributes to the knowledge and genetic characterization of China native breeds.

• 혜화의학회지
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• 제12권2호
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• pp.41-61
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• 2004

Jinguiyaolue, written by Zhong-Jing Zhang(張仲景) in East-Han Dynasty of China, is believed to be the professional book about divers diseases of Korean Medicine(韓醫學). As based its theoretical framework upon Nei-Jing(內經) and Nan-Jing(難經), Jinguiyaolue(金匱要略) not only suggests the basic theory about the cause of disease, mechanism of disease(病機), diagnosis and treatment and prevent of divers disease but also constructs foundation for the clinical medicine. Thus, it can be said that Jinguiyaolue(金匱要略), along with Shang-Han-Lun(像寒論) by Zhong-Jing Zhang(張仲景), will be quite worthy in that the book deals with the fundamental theory of Korean Medicine(韓醫學).

• 한국임상수의학회지
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• 제18권1호
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• pp.67-69
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• 2001

A 3 years-old male Tosa dog was referred to Seoul National University Veterinary Medical Teaching Hospital with a history of difficulty in mastication. Clinical signs of dropped jaw, drooling, mild depression and dehydration were observed. According to history taking, physical examination, neurologic examination, complete blood count (CBC), serum chemical profile and radiography, the dog was diagnosed as idiopathic trigeminal nerve paralysis. Electroacupuncture treatment was applied to the dog on local and distal point at an interval of 7 days. Local points were GB-1 (Tong Zi Liao) of gall bladder meridian and ST-7 (Xia Guan) of stomach meridian. Distal points were PC-4 (Xi Men), PC-6 (Nei Guan) of pericardium meridian. Electrical stimulus was performed for 20 minutes at the frequency of 3 Hz, 3 Volts on ST-7. Ten days after the initial electroacupuncture treatment, clinical signs related to trigeminal nerve paralysis were almost disappeared.

• Journal of Acupuncture Research
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• 제21권2호
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• pp.235-260
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• 2004

Objective : To review was to study acupuncture and moxibustion treatments for abnormal leukorrhea. Normal leukorrhea is always secreted in female vagina. It increased usually by infection (Trichomoniasis and Candidiasis), turmor and so on. Methods : We extracted the parts and acupuncture and moxibustion leukorrhea from ancient and modern oriental medical literature. We have got compared and analyzed 45 kinds of books since Nei Jing(內經). Results and Conclusion : In acupuncture therapy meridians of Bladder, Stomach, Spleen and Conception vessel were mostly used for them in connection with the functions of each meridians. The acupoint used on acupunture only were 78 acupoints. In particular, Chung-guk(cv3), Kwan-won(cv4) point of Conception vessel Meridian and Samum-gyo point of Spleen meridian are frequently applied in leukorrhea.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.211-215
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• 2004

Genetic diversity within the natural populations of Daba ecorace of Antheraea mylitta Drury was studied using individual silkworms collected from the South Singhbhum district of Jharkhand state of India with 21 inter simple sequence repeat (ISSR) primers. A total of 148 bands were produced, of which 79% was polymorphic. The pair wise genetic distance among the individuals varied from 0.186 to 0.329. The dendrogram grouped the individuals into 3 major clusters. Nei's heterozygosity analysis revealed 0.265 ${\times}$ 0.18 variability within the population. The high genetic variability present within the natural population of Daba ecorace of A. mylitta is indicative of their adaptational strategy in nature and have much importance for in situ conservation as well as utilization in breeding programs.

• 한국동물위생학회지
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• 제33권4호
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• pp.353-359
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• 2010

Nucleotide sequences of mitochondrial DNA (mtDNA) of 19 leopard cats (Prionailurus bangalensis euptilura) obtained from Seoul grand park zoo in South Korea were determined for analysing genetic diversity. In the leopard cats, 3 haplotypes of the partial cytochrome b sequences (603 base-pairs, bp) were identified. Haplotypes obtained from those genes showed existences of at least 3 maternal lineages of leopard cats in Korea. Tamura-Nei nucleotide distance among 3 haplotypes were 0.00. Molecular phylogenetic tree showed the similar clustering of haplotypes for genes. Meanwhile, no individual variations within the leopard cats in S. Korea. Genetic surveillance system of leopard cats in S. Korea is warranted for maintaining biological conservation.

• Journal of Microbiology and Biotechnology
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• 제20권9호
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• pp.1266-1275
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• 2010

Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderma species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species.

• International Journal of Industrial Entomology and Biomaterials
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• 제24권2호
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• pp.57-61
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• 2012

The Indian Oak Tasar silkworm, Antheraea proylei J. is a beneficial insect with great economic importance in India for its silk production. In this study, six populations of Antheraea proylei and A. frithi Moore (as an out group) were subjected to inter simple sequence repeat (ISSR) marker analysis in order to assess its genetic diversity. Fifteen ISSR primers produced 91 markers among different breeds of A. proylei and A. frithi of which 89 are polymorphic, generating 97.8% polymorphism. The dendrogram constructed using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and cluster analysis made using Nei's genetic distance resulted in the formation of one major group containing four sub-groups separating the breeds. This result suggests that ISSR amplification is potentially useful for molecular characterization of oak tasar silkworm genotypes.