Genetic Variability in the Natural Populations of Daba Ecorace of Tasar Silkworm (Antheraea mylitta Drury), as Revealed by ISSR Markers

  • Mohandas, T.P. (SeriBiotech Research Laboratory, Central Silk Board) ;
  • Vijayan, K. (SeriBiotech Research Laboratory, Central Silk Board) ;
  • Kar, P.K. (Central Sericultural Germplasm Resources Centre) ;
  • Awasthi, A.K. (SeriBiotech Research Laboratory, Central Silk Board) ;
  • Saratchandra, B. (SeriBiotech Research Laboratory, Central Silk Board)
  • Published : 2004.06.01

Abstract

Genetic diversity within the natural populations of Daba ecorace of Antheraea mylitta Drury was studied using individual silkworms collected from the South Singhbhum district of Jharkhand state of India with 21 inter simple sequence repeat (ISSR) primers. A total of 148 bands were produced, of which 79% was polymorphic. The pair wise genetic distance among the individuals varied from 0.186 to 0.329. The dendrogram grouped the individuals into 3 major clusters. Nei's heterozygosity analysis revealed 0.265 ${\times}$ 0.18 variability within the population. The high genetic variability present within the natural population of Daba ecorace of A. mylitta is indicative of their adaptational strategy in nature and have much importance for in situ conservation as well as utilization in breeding programs.

Keywords

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