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http://dx.doi.org/10.7852/ijie.2012.24.2.057

Genetic Diversity among Indian Oak Tasar Silkworm, Antheraea proylei J. Revealed by ISSR Markers  

Devi, Kanghujam Ibsorani (Department of Zoology, Gauhati University)
Ponnuvel, Kangayam M. (Seri-Biotech Research Laboratory, Central Silk Board)
Singh, Laishram Somen (Regional Tasar Research Station, Central Silk Board)
Singh, Kangjam Chaoba (Regional Tasar Research Station, Central Silk Board)
Dutta, Karabi (Department of Zoology, Gauhati University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.24, no.2, 2012 , pp. 57-61 More about this Journal
Abstract
The Indian Oak Tasar silkworm, Antheraea proylei J. is a beneficial insect with great economic importance in India for its silk production. In this study, six populations of Antheraea proylei and A. frithi Moore (as an out group) were subjected to inter simple sequence repeat (ISSR) marker analysis in order to assess its genetic diversity. Fifteen ISSR primers produced 91 markers among different breeds of A. proylei and A. frithi of which 89 are polymorphic, generating 97.8% polymorphism. The dendrogram constructed using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and cluster analysis made using Nei's genetic distance resulted in the formation of one major group containing four sub-groups separating the breeds. This result suggests that ISSR amplification is potentially useful for molecular characterization of oak tasar silkworm genotypes.
Keywords
Antheraea proylei; ISSR; polymorphism; dendrogram;
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