• The Korean Journal of Physiology and Pharmacology
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• v.24 no.1
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• pp.111-119
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• 2020

In vascular smooth muscle, K⁺ channels, such as voltage-gated K⁺ channels (Kv), inward-rectifier K⁺ channels (Kir), and big-conductance Ca²⁺-activated K⁺ channels (BK_Ca), establish a hyperpolarized membrane potential and counterbalance the depolarizing vasoactive stimuli. Additionally, Kir mediates endothelium-dependent hyperpolarization and the active hyperemia response in various vessels, including the coronary artery. Pulmonary arterial hypertension (PAH) induces right ventricular hypertrophy (RVH), thereby elevating the risk of ischemia and right heart failure. Here, using the whole-cell patch-clamp technique, we compared Kv and Kir current densities (I_Kv and I_Kir) in the left (LCSMCs), right (RCSMCs), and septal branches of coronary smooth muscle cells (SCSMCs) from control and monocrotaline (MCT)-induced PAH rats exhibiting RVH. In control rats, (1) I_Kv was larger in RCSMCs than that in SCSMCs and LCSMCs, (2) I_Kv inactivation occurred at more negative voltages in SCSMCs than those in RCSMCs and LCSMCs, (3) I_Kir was smaller in SCSMCs than that in RCSMCs and LCSMCs, and (4) I_BKCa did not differ between branches. Moreover, in PAH rats, I_Kir and I_Kv decreased in SCSMCs, but not in RCSMCs or LCSMCs, and I_BKCa did not change in any of the branches. These results demonstrated that SCSMC-specific decreases in I_Kv and I_Kir occur in an MCT-induced PAH model, thereby offering insights into the potential pathophysiological implications of coronary blood flow regulation in right heart disease. Furthermore, the relatively smaller I_Kir in SCSMCs suggested a less effective vasodilatory response in the septal region to the moderate increase in extracellular K⁺ concentration under increased activity of the myocardium.

• Korean Journal of Cognitive Science
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• v.20 no.1
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• pp.51-77
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• 2009

Using a modified Posner's cue-target paradigm, we investigated whether negative cues attract more attention than neutral cues in anxious people. Previous studies used commonly an unbalanced proportion of valid and invalid trials(75% vs. 25% respectively). But in the present study, an equivalent proportion of valid and invalids trials was used for measuring detection speed of cues without participant's expectancy caused by the unbalanced proportion. Emotional words(Experiment 1) and facial expressions(Experiment 2) were used as cues for target locations. The result of Experiment 1 and 2 showed that threatening cues facilitated target detection in valid trials and interfered with it in invalid trials in anxious participants and a, reverse response patterns were found in non-anxious participants. This indicates that threatening cues attract more attention to the cued location in anxious people and in contrast, non-anxious people avoid threatening stimuli. In Experiment 3, we investigated the difference of validity effect across anxiety levels. The results showed that anxious participants gave less attention to cued location when the cues were non-informative whereas non-anxious participants gave more attention to cued locations in the same condition. We discussed two kinds of cognitive bias caused by anxiety levels: attentional bias and proportion related bias.

• Science of Emotion and Sensibility
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• v.13 no.1
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• pp.179-186
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• 2010

The present study is a partial extension of the Duncan-Johnson and Donchin (1979) and Soskins, Rosenfeld, and Niendam (2001) studies that found some effects of high pass filter (HPF) setting on P300 waveform. EEGs were recorded while the subjects performed a visual three-stimulus oddball task, and the Fz and Pz EEG signals were passed through 0.01 Hz, 0.3 Hz, and 1.0 Hz HPFs. P300 amplitudes at Fz and Pz were reduced at 1.0 Hz HPF compared to 0.01 Hz and 0.3 Hz HPFs. The negative peaks post P300 at Fz and Pz were not observed at 0.01 Hz HPF, but observed at 0.3 Hz and 1.0 Hz HPFs. The combination of 0.3 Hz HPF and peak-to-peak P300 measures was more useful than that of 0.01 Hz, 0.3 Hz, or 1.0 Hz HPF and baseline-to-peak P300 measures to discriminate between non-target and standard stimuli. The peak-to-peak P300 measures were more useful than the baseline-topeak P300 measures at 1.0 Hz HPF setting.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.299-323
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• 2018

This study confirmed the life career branching points, and studied qualitatively the career strategy and career identity at that time. The participants in this study were three students from third graders of middle school, three ones from third graders of high school, and three before and after college graduation, which correspond to the vertical transition stage of school education. Three participants were selected before and after 30 years of age, three before and after 40 years of age, and three before and after 60 years of age. Subject analysis of the contents of the 18 interviews, showed that the life career branching point appeared in middle school grade 3, high school grade 3, until employment after graduation, within 3 years after entering their first job, early 40 years, 60 years old, and 80 years old. Second, external situations were due to the influence of important others, or external stimuli, environment, and career events. Third, negative emotions were repeated for each life career branching point. Fourth, as a result of the interview, the career identity confusion was repeated in every life career branching point. Fifth, the career strategy at the life career branching point was categorized as an approach strategy and avoidance strategy.

• Journal of Korean Academy of Nursing
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• v.29 no.6
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• pp.1365-1378
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• 1999

This study is a methodological research study to develop an instrument to measure in patients with cancer and to test the validity and reliability of the instrument. The research procedure was as follows : 1) The first step was to develop conceptual framework based on a comprehensive review of the literature and in-depth interviews with patients with cancer. This conceptual framework was organized in to three dimensions (the intrapersonal dimension, the significant-other and context related dimension, the transcendental dimension). Initially 59 items were adopted. 2) These items were analyzed through the index of content validity(CVI) and 53 items were selected which met more than 80% on the CVI. 3) The pretest was carried out with 87 patients with cancer. After the pretest results were analyzed by item analysis, 44 items were selected. A second test of content validity was conducted and 6 items were eliminated considering the 80% CVI. 4) To test for reliability and validity, data collection was done during the period from January 25, 1999, to February 26, 1999. The subjects for the test were 160 patients with cancer and 185 healthy persons. analysis, item analysis and multitrait-multimethod method to analyze validity. The findings are as follows : 1) The Cronbach's alpha coefficient for internal consistency was .92 for the total 38 items and .79, .82, .85, for the three dimensions in that order. 2) The item analysis was based on the corrected item to total correlation coefficient( .30 or more) and information about the alpha estimate if this item was dropped from the scale. 3) As a result of the initial factor analysis using principal component analysis and varimax rotation, one item was deleted because of factor complexity (indiscriminate factor loadings). In the secondary factor analysis, 7 factors with eigenvalue of more than 1.0 were extracted and these factors explained 56 percents of the total variance. The seven factors were labeled as 'family relationship', 'emotional condition', 'physical discomfort', 'meaning and goal of life', 'contextual stimuli', 'change of body image', 'guilt feelings'. 4) The convergence effect between this instrument and the life satisfaction scale was identified and there was significant positive correlation(r= .52, p= .00). The discriminant validity between this instrument and the depression scale(CES-D) was tested and there was significant negative correlation(r= -.50, p= .00). The instrument for accessing the suffering of patients with cancer developed in this study was identified as a tool with a high degree of reliability and validity. In this sense, this tool can be effectively utilized for assessment in caring for patients with cancer.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.349-361
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• 2010

In this review, the current knowledge of the carbon metabolism and global carbon regulation in Corynebacterium glutamicum are summarized. C. gluamicum has phosphotransferase system (PTS) for the utilization of sucrose, glucose, and fructose. C. glutamicum does not show any preference for glucose when various sugars or organic acids are present with glucose, and thus cometabolizes glucose with other sugars or organic acids. The molecular mechanism of global carbon regulation such as carbon catabolite repression (CCR) in C. glutamicum is quite different to that in Gram-negative or low-GC Gram-positive bacteria. GlxR (glyoxylate bypass regulator) in C. glutamicum is the cyclic AMP receptor protein (CRP) homologue of E. coli. GlxR has been reported to regulate genes involved in not only glyoxylate bypass, but also central carbon metabolism and CCR including glycolysis, gluconeogenesis, and tricarboxylic acid (TCA) cycle. Therefore, GlxR has been suggested as a global transcriptional regulator for the regulation of diverse physiological processes as well as carbon metabolism. Adenylate cyclase of C. glutamicum is a membrane protein belonging to class III adenylate cyclases, thus it could possibly be a sensor for some external signal, thereby modulating cAMP level in response to environmental stimuli. In addition to GlxR, three additional transcriptional regulators like RamB, RamA, and SugR are also involved in regulating the expression of many genes of carbon metabolism. Finally, recent approaches for constructing new pathways for the utilization of new carbon sources, and strategies for enhancing amino acid production through genetic modification of carbon metabolism or regulatory network are described.

• Journal of the Korea Computer Graphics Society
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• v.26 no.3
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• pp.143-153
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• 2020

This study embodies and proposes VR color therapy healing contents 'Nornir' that can manage stress in daily life. "Nornir" applies the CRR analysis method to provide a customized VR color therapy experience according to the three colors selected by the user. It is designed to enable users to understand themselves through their color journey, to rec eive various color interactions and stimuli to implement in the future, and to provide healing that lowers stress levels. Based on the results implemented, the Korean version of the mood condition test 'K-POMS' was conducted before an d after the demonstration to check the user's stress changes after the content demonstration. Experiments have shown that users clearly see a decrease in negative emotions and an increase in positive emotions. By using VR technology, color psychotherapy rules are combined to provide the possibility of relieving stress for users who are exposed to fre quent stress in daily life.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.895-903
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• 2005

Thrombospondin-1 (TSP-1), a negative regulator in tumor growth and angiogenesis, is cell-type specifically regulated under pathological conditions or by extracellular stimuli, and the regulation of TSP-1 gene expression is important for developing new approaches in tumor therapy. Mistletoe is a parasitir plant that have been used for immunomodulation and antitumor therapy. Helixor A is an aqueous part of mistletoes extract. Here we showed that TSP-1 expression was significantly induced at both mRNA and protein levels in the Hepatocarcinorna cell line (Hep3B) and primary bovine endothelial cell line (BAE) exposed to Helixor A. Our promoter analysis confirmed that the expression of TSP-1 gene was regulated by Helixor A at the transcriptional level. In cell invasion assay, the conditioned media obtained from treatment of these cells significantly reduced the number of invasive cells and also inhibited capillary-like tube formation of BAE cells on Matrigel. Moreover, the inhibitory efforts of the conditioned media on cell invasion and tube formation were reversed by blocking with anti-TSP-1 neutralizing antibodies, suggesting that TSP-1 is involved in Helixor A-indured antiangiogenic effect. Taken together, our results suggest that Helixor A have an antiangiogenic effects through upregulation of TSP-1.

• Korean Journal of Cognitive Science
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• v.30 no.1
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• pp.31-50
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• 2019

The present study is designed to investigate the effect of perceptive, emotional and semantic distinctiveness on retrieval-induced forgetting(RIF). Experiment 1 was designed to construct a category and category list for RIF experimental paradigm and to investigate the effects of perceptual distinctness on retrieval-induced forgetting. It was used for the list consisting of the six categories and six words in each category list. In controlled conditions, all the stimuli were presented in black and Gothic. In contrast, perceptual distinctiveness conditions, half of the category list were presented in red and Gungseoche. RIF was observed in all conditions. Experiment 2 was designed to investigate the effects of semantic and emotional distinctiveness on retrieval-induced forgetting. In neutral conditions, adjectives related to items were added. In the emotional distinctiveness condition, half of the items in the category were manipulated in such a way as to add the negative adjectives. In the semantic distinctiveness condition, half of the items in the category were manipulated in such a way as to add the inappropriate adjective. As a result, RIF occurred in the neutral condition, but RIF did not occur in both the emotional discrimination condition and the semantic discrimination. These results suggest the possibility that the RIF will not occur when the distinctiveness occurs within a categorical relationship.

• Phonetics and Speech Sciences
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• v.14 no.4
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• pp.1-10
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• 2022

The Korean polysemic ending '-(eu)lgeol' can has two different meanings, 'guess' and 'regret'. These are expressed by different boundary-tone types: a rising tone for guess, a falling one for regret. Therefore the sentence-final boundary-tone type is the most salient prosodic feature. However, besides tone type, the pitch difference between the final and penultimate syllables of '-(eu)lgeol' can also affect semantic discrimination. To investigate this aspect, we conducted a perception test using two sentences that were morphologically and syntactically identical. These two sentences were spoken using different boundary-tone types by a Korean native speaker. From these two sentences, the experimental stimuli were generated by artificially raising or lowering the pitch of the boundary syllable by 1Qt while fixing the pitch of the penultimate syllable and boundary-tone type. Thirty Korean native speakers participated in three levels of perceptual test, in which they were asked to mark whether the experimental sentences they listened to were perceived as guess or regret. The results revealed that regardless of boundary-tone types, the larger the pitch difference between the final and penultimate syllable in the positive direction, the more likely it is perceived as guess, and the smaller the pitch difference in the negative direction, the more likely it is perceived as regret.