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Isolation and Characterization of Bacteriophages Against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit

  • Yu, Ji-Gang;Lim, Jeong-A;Song, Yu-Rim;Heu, Sunggi;Kim, Gyoung Hee;Koh, Young Jin;Oh, Chang-Sik
    • Journal of Microbiology and Biotechnology
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    • v.26 no.2
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    • pp.385-393
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    • 2016
  • Pseudomonas syringae pv. actinidiae causes bacterial canker disease in kiwifruit. Owing to the prohibition of agricultural antibiotic use in major kiwifruit-cultivating countries, alternative methods need to be developed to manage this disease. Bacteriophages are viruses that specifically infect target bacteria and have recently been reconsidered as potential biological control agents for bacterial pathogens owing to their specificity in terms of host range. In this study, we isolated bacteriophages against P. syringae pv. actinidiae from soils collected from kiwifruit orchards in Korea and selected seven bacteriophages for further characterization based on restriction enzyme digestion patterns of genomic DNA. Among the studied bacteriophages, two belong to the Myoviridae family and three belong to the Podoviridae family, based on morphology observed by transmission electron microscopy. The host range of the selected bacteriophages was confirmed using 18 strains of P. syringae pv. actinidiae, including the Psa2 and Psa3 groups, and some were also effective against other P. syringae pathovars. Lytic activity of the selected bacteriophages was sustained in vitro until 80 h, and their activity remained stable up to 50℃, at pH 11, and under UV-B light. These results indicate that the isolated bacteriophages are specific to P. syringae species and are resistant to various environmental factors, implying their potential use in control of bacterial canker disease in kiwifruits.

A Study on the Architectural Education for the Development of the Architectural Form with Using Digital Design Tools (디지털 디자인 도구를 이용한 건축형태개발을 위한 건축교육에 관한 연구)

  • Jeong J.-S.;Choi M.-H.
    • Korean Journal of Computational Design and Engineering
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    • v.9 no.4
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    • pp.334-350
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    • 2004
  • Until now, architects have used traditional methods in order to develop architectural forms and use a digital technology as presentation tools. The field of digital technology has been expanded to control easily architectural data, eventually leads to the creation for creative ideas, which were belong to mainly designer's talent. It has been educated by using digital tool in foreign universities but in our case a little university try to do. So we need an adequate curriculum and educational media because of digitalized data, originated architectural form and reduction of time and money. In this paper, as it is surveyed the present situation of architetural education by using digital tool in domestic and foreign country, it can be used as the fundamental data that architectural education by using digital tool is initiated In our universities.

Using Mean Shift Algorithm Enhance Edge Detection Effect (에지 추출 향상을 위한 Mean Shift 알고리즘의 이용)

  • Lei, Wang;Shin, Seong-Yoon;Rhee, Yang-Won
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2009.01a
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    • pp.211-214
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    • 2009
  • Edge detection always influenced by noise belong to the original image, therefore need use some methods to sort this issue, mean shift algorithm has the smooth function which suit for the edge detection purpose, so adopted to fade out the unimportant information, and the sensitive noise portions. After this section, use the Canny algorithm to pick up the contour of the objects we focus on, meanwhile select the Soble operator that has the orientation attribute to support the method work well. In final, take experiment and get the perfect result we wanted, make sure this method make sense and better than the sole Edge detection algorithm,

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The Study on Consistency of Simulation Logic about Close Combat Damage Assessment among Constructive Models : Based on Combined Arms Integrated Interoperability System (워게임모델간 근접전투 피해평가 모의논리 일치에 관한 연구 : 제병협동통합연동체계를 중심으로)

  • Moon, Ho-Seok;Kim, Hyung-Se;Hwang, Myung-Sang;Bae, Hyun-Wung;Lee, Dong-Keun
    • Journal of the military operations research society of Korea
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    • v.37 no.1
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    • pp.87-97
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    • 2011
  • In this paper, we propose a new close combat expert system to overcome the difference of combat damage assessments between combat units belong to their own model in Combined Arms Integrated Interoperability System(CAIIS) which will be deployed in the early future. When it happens to engage in a battle among combat units belong to their own model in CAIIS, the result of damage assessment is different severely. This is related to CAIIS's confidence and need to be overcome. We propose the expert system for close combat damage assessment with a decision tree. Simulation results show that the proposed expert system is valid well. Because the proposed expert system is made not as an independent system but as an inner module type of CAIIS, CAIIS will be simpler system than we expect. And we will hope to reduce the cost of CAIIS.

A Study of Disk-Shaped Bronze Implements (부록 1. 원개형동기의 앞뒷면 - 그 사용법에 관하여 -)

  • Shimizu, Yasuji
    • Korean Journal of Heritage: History & Science
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    • v.39
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    • pp.281-314
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    • 2006
  • Several explanations exist about the usage of disk-shaped bronze implements in the ancient society. Some argue that they were used as mirrors, others suggest percussion instruments, and still others bronze ornaments. Differences between disk-shaped bronze implements and mirrors with multiple knobs are that the former have no visible design, only one handle, and a sharp curvature unlike the latter with smooth curvature. The question is whether disk-shaped bronze implements excavated from Korean peninsula have any light reflecting function. To answer this question, I carefully studied the concave surfaces of disk-shaped bronze implements excavated from Goejeong-dong, Tongso-ri and Hapsong-ri sites. The main findings are as follows: (1) The concave sides of the disk-shaped bronze implements excavated from Goejeong-dong and Tongso-ri sites were highly polished, and they were as reflective as bronze mirrors. (2) The concave side of the disk-shaped bronze implement from Hapsong-ri site was unpolished, and it was different from bronze mirrors. (3) As for the convex sides of the disk-shaped bronze implements, they did not appear to have been polished with care. Considering the above findings, the disk-shaped bronze implements excavated from Goejeong-dong and Tongso-ri sites belong to the stage where they could act as both mirrors and instruments. On the contrary, the disk-shaped bronze implement from Hapsong-ri site can belong to the stage where it lost the function of being a mirror as the result of maintaining only its function as an instrument. Even though disk-shaped implements had two functions, it can be considered that the light reflecting function expanded the role of a mirror with multiple knobs and the sound function helped the engraved round bronze implement to be converted into an eight-armed bronze rattle. Since it has been reported that shamans used bronze mirrors as percussion instruments in their performances in Korean peninsula and Siberia, I propose a reconsideration of the usage of mirrors in the ancient East Asia. Although the essential function of a mirror is to reflect light, other possible usages involving important functions need to be further investigated.

Feature Expansion based on LDA Word Distribution for Performance Improvement of Informal Document Classification (비격식 문서 분류 성능 개선을 위한 LDA 단어 분포 기반의 자질 확장)

  • Lee, Hokyung;Yang, Seon;Ko, Youngjoong
    • Journal of KIISE
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    • v.43 no.9
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    • pp.1008-1014
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    • 2016
  • Data such as Twitter, Facebook, and customer reviews belong to the informal document group, whereas, newspapers that have grammar correction step belong to the formal document group. Finding consistent rules or patterns in informal documents is difficult, as compared to formal documents. Hence, there is a need for additional approaches to improve informal document analysis. In this study, we classified Twitter data, a representative informal document, into ten categories. To improve performance, we revised and expanded features based on LDA(Latent Dirichlet allocation) word distribution. Using LDA top-ranked words, the other words were separated or bundled, and the feature set was thus expanded repeatedly. Finally, we conducted document classification with the expanded features. Experimental results indicated that the proposed method improved the micro-averaged F1-score of 7.11%p, as compared to the results before the feature expansion step.

An Optimal Incentive-Compatible Pricing for Congestible Networks (혼잡이 있는 네트워크를 위한 동기 유발 가격)

  • Kim, Yong-Jae
    • Asia pacific journal of information systems
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    • v.7 no.3
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    • pp.109-124
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    • 1997
  • Pricing information services, where congestion can threaten the efficient operation of information systems, has been studied in economics and information systems literature, Recent explosion of the Internet ana proliferation of multimedia content over the Internet have rekindled the research interest in designing pricing schedules for differentiated information services, In order for the information system to effectively serve users having heterogeneous needs, pricing rules for discriminated services should be considered. At the same time, when individual users' interest does not align with that of the organization that individual users belong to, organization-wide pricing policy should be devised to improve the value of the services rendered by the system. This paper, using a priority queuing model, addresses the need for such on incentive-compatible pricing for different information services.

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A Shaking Optimization Algorithm for Solving Job Shop Scheduling Problem

  • Abdelhafiez, Ehab A.;Alturki, Fahd A.
    • Industrial Engineering and Management Systems
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    • v.10 no.1
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    • pp.7-14
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    • 2011
  • In solving the Job Shop Scheduling Problem, the best solution rarely is completely random; it follows one or more rules (heuristics). The Genetic Algorithm (GA), Particle Swarm Optimization (PSO), Simulated Annealing, and Tabu search, which belong to the Evolutionary Computations Algorithms (ECs), are not efficient enough in solving this problem as they neglect all conventional heuristics and hence they need to be hybridized with different heuristics. In this paper a new algorithm titled "Shaking Optimization Algorithm" is proposed that follows the common methodology of the Evolutionary Computations while utilizing different heuristics during the evolution process of the solution. The results show that the proposed algorithm outperforms the GA, PSO, SA, and TS algorithms, while being a good competitor to some other hybridized techniques in solving a selected number of benchmark Job Shop Scheduling problems.

A Study on the Marketing Management Strategy of Special Libraries in Korea (전문도서관 마케팅 경영전략)

  • Lee, Yong-Jae
    • Journal of Korean Library and Information Science Society
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    • v.38 no.3
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    • pp.335-351
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    • 2007
  • In the knowledge-based society of 21st century, special libraries face intense competitive environment. So they need to do 'library marketing' aimed at the organizations which they belong to and customers whom they serve for their survival and success. This study suggests the concept of 'library marketing', the cases of 'library marketing' at home and abroad, and concrete marketing management strategies for special libraries in Korea.

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Fandom as a Prosumer : Study on Information Behavior of Fandom (프로슈머로서의 팬덤: 팬덤의 정보행동에 관한 연구)

  • Lee, So Young;Kim, Hyang Mi;Chu, Kyounghee;Seo, Jungchi
    • Journal of Digital Convergence
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    • v.11 no.12
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    • pp.747-759
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    • 2013
  • This study posits the activities of the fandom as information behavior: information producing, information diffusion, information sharing. The authors identify the underlying motivation and needs which lead fandom to these behavior on the basis of contents theory. In particular, the manner in which the those needs influence the information behavior of fandom is explored. The data used in this study came from various Fan Caf$\acute{e}$s which are communities of star fan. The results from the survey shows that the fandom is a kind of culture closely connected to social needs. Fandom can be viewed as an important driving force in the entertainment industry as they are co-creating the value in entertainment market.