• Journal of Microbiology and Biotechnology
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• v.25 no.4
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• pp.511-520
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• 2015

Triclosan, the widely used biocide, specifically targets enoyl-acyl carrier protein reductase (ENR) in the bacterial fatty acid synthesis system. Although the fish pathogen Aeromonas salmonicida subsp. salmonicida exhibits triclosan resistance, the nature of this resistance has not been elucidated. Here, we aimed to characterize the triclosan resistance of A. salmonicida subsp. salmonicida causing furunculosis. The fosmid library of triclosan-resistant A. salmonicida subsp. salmonicida was constructed to select a fosmid clone showing triclosan resistance. With the fosmid clone showing triclosan resistance, a subsequent secondary library search resulted in the selection of subclone pTSR-1. DNA sequence analysis of pTSR-1 revealed the presence of a chromosomal-borne fabV-encoding ENR homolog. The ENR of A. salmonicida (FabVas) exhibited significant homology with previously known FabV, including the catalytic domain YX₍₈₎K. fabVas introduction into E. coli dramatically increased its resistance to triclosan. Heterologous expression of FabVas might functionally replace the triclosan-sensitive FabI in vivo to confer E. coli with triclosan resistance. A genome-wide search for fabVas homologs revealed the presence of an additional fabV gene (fabVas2) paralog in A. salmonicida strains and the fabVas orthologs from other gram-negative fish pathogens. Both of the potential FabV ENRs expressed similarly with or without triclosan supplement. This is the first report about the presence of two potential FabV ENRs in a single pathogenic bacterium. Our result suggests that triclosan-resistant ENRs are widely distributed in various bacteria in nature, and the wide use of this biocide can spread these triclosan-tolerant ENRs among fish pathogens and other pathogenic bacteria.

• Archives of Pharmacal Research
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• v.16 no.3
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• pp.219-226
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• 1993

For the analysis of structure relationship of ar-turmerone analogues, the compounds containing the various substituents on the phenyl ring and 1(or 2)-naphthyl group in the place of phenyl of ar-turmerone were prepared and tested their cytotoxicity against HL-60, K-562, and L1210 leukemia cells in vitro. The substituents at para position are methoxy, phenoxy, methyl, trifluoromethyl, fluoro, and chloro. At meta position methoxy, methyl, trifluoromethyl, or chloro groups at ortho position mathoxy or chloro group were introduced. Against HL-60 and K-562 cells, $ED_{50}$ values of the analogues are ranged from 0.8 to $30.0\;\mu{g/ml}$. Againste L1210 cell, these are located more than $20.0\;\mu{g/ml}$. However, 5-carbone-thoxy-2-methyl-6(1-naphthyl)-2-octen-4-one (5n)possesses $ED_{50}$ valuses 0.8, 2.1, $6.5\;\mu{g/ml}$ against HL-60, L1210 cells, respectively. The electronic nature of the substituents on phenyl ring of ar-tumerone dose not affect the biological activity. Therefore the flat structure of aromatic potion of ar-tumerone analogues is the more important factor for their activity rather than its electronic nature. The potentiation of the cytotoxicity with the enlargement of aromatic ring region also supports the importance of the plane structure of this area. The restriction of the single bond rotation between C-6 and aromatic ring through the introduction of substituents at the ortho position of phenyl ring and the increment of size of alkyl group at C-6 position enhances the activity. Therefore the effective conformation should by the one having the orthogonal arrangement between the aromatic ring and the side chain.

• The Journal of Fisheries Business Administration
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• v.34 no.1
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• pp.69-85
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• 2003

The primary objective of this thesis is to study, case by case, the international disputes for fishing rights between fishing nations and costal states never imagined till the introduction of the UN Convention on the Law of the Sea adopted in 1982 and came into effect in November 16, 1994, which governs the high seas and EEZ in a new manner. Such a study is to provide help in the understanding on this new marine system and how to deal with. This is addressed by the perspectives of disputes (a) in the high seas between fishing nations having traditionally enjoyed the principle of the freedom of the high seas and costal states, (b) in the EEZ between fishing nations and costal states possessing the exclusive jurisdiction over living marine resources and sovereign rights for determining allowable catch and the surplus in its EEZ. The article can be divided into four main parts. First, both the general principles of the settlement of international disputes, and the nature and procedures described in the UNCLOS are introduced. Second, it gives cases of tuna long-liner, North Pacific trawler and squid jigger occurred in the coastal states EEZ, and analyses the problem in both terms of its background and final judgment. It further describes the possible issues in case it depends on the International Tribunal for the Law of the Sea for its settlement. Third, closely tied to above, important points such as the right of hot pursuit, prompt release of vessel and crew, and the limits of cooperation with costal states inspector on board fishing vessels are considered mostly based on the UNCLOS, Bilateral Agreement and UNIA. Finally, the article concludes as follows ; The need for broad analyses on the nature of international suits and legal system for the settlement, to win the case before the International Tribunal for the Law of the Sea or coastal states court, is really acknowledged. However, considering the lack of previous studies about it, it is preferably recommended that governmental efforts for making legal standards to cover the judicial costs, for helping industry out of becoming bankrupt.

• Journal of Korean Philosophical Society
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• v.144
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• pp.33-50
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• 2017

Confucianism acts as the representative of Chinese mainstream cultures. Its research on the relation between groups and individuals, and about norms of the system is not only complete but of a long history. And its abundant management thoughts are important assets for us to know traditional culture and construct the management study of local colors. Menzi and Xunzi both inherit Confucianism but evolve into two Confucian paths of different scenarios. Both of them are different despite sameness, and are the same despite differences. Both are artistic all right, but have their imitation. This article aims to use Mengzi thoughts and Xunzi thoughts as a study domain. The study includes five sections. The first section as an introduction presents problems. The second section uses extrinsic factors to research the birth ground of Menzi, Xunzi management thoughts. The third section examines its basic presumptions in terms of inner logic. The fifth section analyzes its logical structure, and in this section the previous sections are concluded, presenting the possible development and relative limitations of Mengzi, Xunzi management thoughts. It is expected that we can re-examine traditional Confucius thoughts from the angle of management, renewing Confucius thoughts constantly in different ages.

• Journal of People, Plants, and Environment
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• v.24 no.1
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• pp.29-38
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• 2021

Background and objective: The physical environment where children spend most of their time is closely associated with their emotional development. To improve the emotional health of children, the introduction of natural elements in the indoor space has been suggested, the benefits of which have been shown in preliminary studies. This study aims to examine the effects that a biowall - a wall installation - in a classroom has on the emotional health of children in kindergarten. Methods: A total of four biowalls were separately installed in four kindergarten classrooms at a school in Seoul, South Korea, and the 60 children in these classrooms participated in the study. We assessed the children's emotional intelligence (via an Emotional Intelligence Rating Scale), resilience (via the Devereux Early Childhood Assessment), and eco-friendly attitudes (via the Children's Attitudes Toward Scale) before the installation of the biowalls and then again 3 months later. Results: The children's emotional intelligence, resilience, and eco-friendly attitudes had been significantly improved after the installation of the biowalls (p = .01). The sub-categories of the children's emotional intelligence and resilience were also significantly improved (p < .001). Conclusion: This study demonstrates the potential of biowalls-as an indoor environmental factor-in promoting the healthy emotional development of children. By bringing natural elements into indoor classroom settings, biowalls appear to increase children's direct/indirect contact with nature. To extrapolate the results of the study to the general population, future studies should be conducted with broader age groups.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.23 no.6
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• pp.103-108
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• 2023

This paper conveys the limitations of analog natural history sample information in digital form, giving viewers interest and fun, and presenting the direction of digital diorama utilization of sample exhibitions. In this exhibition, through various media such as 10,000 won bill dramas and newspaper articles, the Sun and Moon and Five Peaks, a picture familiar to the public, was reproduced in media art so that it can be realized in real life. It is a Joseon Dynasty work in which five mountain peaks, a pair of waterfalls, and four pine trees are drawn symmetrically from side to side. In order to express the vividness of nature, the symbols of the sun and moon were created with the effect of light to maximize immersion, and animals such as waves, crane movements, deer, ramie butterflies, and carp were inserted under the mountain peaks to create the vividness of nature and creatures playing in them. The media art folding screen was produced and directed as a screen. In addition, the introduction of the work and information related to the living things in the work were provided through QR codes.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.34 no.2
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• pp.229-244
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• 2024

Recently, supply chain attacks against nuclear facilities such as "Evil PLC" are increasing due to the application of digital technology in nuclear power plants such as the APR1400 reactor. Nuclear supply chain security requires a asset management system that can systematically manage a large number of providers due to the nature of the industry. However, due to the nature of the control system, there is a problem of inconsistent management of attribute information due to the long lifecycle of software assets. In addition, due to the availability of the operational technology, the introduction of automated configuration management is insufficient, and limitations such as input errors exist. This study proposes a systematic asset management system using SBOM(Software Bill Of Materials) and an improvement for input errors using natural language processing techniques.

• Journal of Advanced Navigation Technology
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• v.28 no.3
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• pp.288-299
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• 2024

Indonesia's Keplauan Liau region is facing limitations in the development of connected transportation infrastructure due to its archipelago nature, budget constraints, and lack of land. Transportation demand is increasing due to its strategic location in the Malacca Strait Business Triangle and many tourist visits from Singapore and Malaysia. However, due to the nature of connecting many islands, the establishment of transportation infrastructure has not been achieved. This paper aims to predict the innovations that can be brought about by the introduction of advanced air mobility (AAM) with an electric vertical take-off and landing (e-VTOL) system through analysis and application consideration of the actual situation in Indonesia's Kepulauan Liau region. In addition, it intends to contribute to national-level review and policy establishment on the establishment of innovative transportation infrastructure using AAM, reflection in infrastructure construction plans, and active global cooperation.

• Korean Journal of Environment and Ecology
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• v.21 no.3
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• pp.223-234
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• 2007

The study, executed with Hallasan National Park, which deserves to be a typical ecotourism destination, aims to provide basic information on park management for early establishment of ecotourism in a national park by assessing its visitors' behavior and satisfaction with a nature trail established as a series of an environmental interpretation program. The questionnaire survey was conducted at Eorimok Square in the weekday and on the weekend for two months of August and September in 2006, and finally 144 valid samples were used for the analysis. As a result of the research, it revealed that the demographic characteristics of the visitors to Hallasan National Park tended to coincide with those of the visitors to other national parks In Korea. On the whole, it showed their low recognition level of nature trails built up in national parks and less experience in using them. However, the visitors' satisfaction level and intention of re-visit, and recommendation to others were comparatively higher after actually using the nature trail at the site of Hallasan National Park, which hints at the possibility of national parks' much weightier role as the ground for ecology education and the functional expansion of the environmental interpretation-related facilities and programs. As for the attributes having effects on users' satisfaction with a nature trail, substantial aspects such as accessibility, safety, uniqueness and interest in environmental interpretation, and educational quality as well as physical facility management were revealed to have equal effects on users' satisfaction level, so there still remain a lot of pending issues over the reality of national parks in the initial stage of ecotourism staying at the level of the introduction and establishment of the facilities for environmental interpretation. This research had surveyed visitors to Hallasan National Park and limited to the nature trail only. For more systematic and practical ecological management of a national park, the in-depth understanding of the attributes affecting satisfaction of ecotourists, including nature trails and other environmental interpretation programs, and more sophisticated measuring tools are needed.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.38
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• pp.229-262
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• 2008

With the introduction of supply chain management concept, the nature of a firm is fundamentally changed. Control is no longer based on direct control of the business processes, but rather based on integration across member organizations in the supply chain. Firms are trying to achieve a competitive edge by managing their supply chain performance to gain advantages in cost and service differentiation. The supply chain elements of a sea port involves ILSP (Inbound Logistics Service Provider) at the input side and OLSP (Outbound Logistics Service Provider) at the output side. The goal of sea port is to satisfy the needs of different parties, both upstream and downstream, in the chain with greater effectiveness and efficiency than it's competitors. In this context, the purpose of this paper is to derive conceptualization of the SCP (Supply Chain Performance) in the sea port from literature review on the previous researches and to tackle their theoretical and practical implications. The paper deals with conceptualizing SCP in sea port using the SCOR (Supply Chain Operation Reference) model. It incorporates both the effectiveness and efficiency aspects of performance measurement. And it also recognizes customer related reasons for measuring performance.