• Journal of Veterinary Clinics
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• v.16 no.1
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• pp.155-162
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• 1999

This study was performed to assess that clinco-therapeutic effect of natural Italian honeybee (Apis mellifera) venom in adjuvant-induced arthritic rat. Ninety Sprague- Dawley rats of male were injected with complete Freund's adjuvant (CFA). Adjuvant arthritis was produced by a single subcutaneous injection of 1 mg Mycobacterium butyricum suspended in 0.1 ml paraffin oil into the right hindpaw. Righting reflex was uniformly lost and considered to be the point of arthritis development on day 14 after CFA injection. Experimental groups were divided into three groups. When arthritis was developed in the rat hind-paw, tested groups were administrated with prednisolone (10 mg/kg, p.o) and honeybee venom (one bee, s.c) at an interval of two days. Control group was subcutaneously injected with 0.1 ml of physiological saline solution in the rat at an interval of two days. Clinical findings, hematological values and histopathological findings were observed during or after the drugs administration. In tested groups, the development of inflammatory edema and polyarthritis on day 14 after treatment was suppressed. No significant differences of hindpaw edema volume and lameness score between prednisolone and honeybee venom groups were observed during or after therapeutic drugs treatment. WBC counts of prednisolone and honeybee venom treatment groups as compared with the control group were getting remarkably decreased during or after the therapeutic drugs administration(p＜0.01). Erosions of articular cartilage and inflammatory cell infiltrations during or after the therapeutic drugs treatment was effectively suppressed in natural honey venom.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.42 no.4
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• pp.1-14
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• 2010

To investigate natural dyeing properties of bast fiber, properties of pre mordanting dyeing of sappan wood, gardenia and gallnut such as K/S values, developed color, and fastness were evaluated. Silk showed the most increased K/S values by pre mordanting treatment when Fe was uses as a mordant. The optimized amount of Fe was 3%. The colors of sappan wood was a series of YR. After pre mordanting treatment with Fe, the colors of bast fiber of mulberry and silk showed a series of RP, and the color of cotton showed a series of Y. The color of gallnut was a series of Y regardless of pre mordant treatment. Sappon wood (YR), gardenia (Y), and gallnut (Y) showed various colors such as YR, R, and RP, respectively. Sunlight fastness was not improved by pre mordanting treatment. The effect of pre mordanting treatment was not good. Especially the treatment with Al showed poor sunlight fastness.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.185-189
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• 1987

The number of splenic lymphocyte, serum prostaglandin $E_2$ level and natural killer cell activity were assayed after single whole body irradiation of a sublethal dose of $^{60}Co-{\gamma}$ ray to C57BL/6J mice. With a view to knowing the relationships between radiation induced prostaglandin $E_2$ level and the normal natural killer cell activity after natural killer cell-target cell conjugation, The change of normal natural killer cell activity were measured by administration of prostaglandin $E_2$ containing serum from irradiated mice. The results were summarized as follows; 1. The total number of splenic lymphocyte was significantly decreased by irradiation and the number was not affected by indometacin, prostaglandin synthesis inhibitor, treatment. 2. Serum prostaglandin $E_2$ level was increased in irradiated mice, but indometacin treated mice group showed low level of prostaglandin $E_2$. 3. In the case of irradiated mice, natural killer cell activity was not shown any difference between irradiated group and indometacin combined group. But when natural killer cell-target cell conjugations were exposed to the serum of each group during cytotoxic activity assay, whereas the normal natural killer cell activity was significantly decreased by treatment of serum from irradiated mice, the activity was not changed by treatment of indometacin pretreated mice serum. This result indicated that the prostaglandin $E_2$ induced by the radiation inhibited the post-target binding cytolytic process of natural killer activity.

• Journal of Korean Society of Forest Science
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• v.106 no.1
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• pp.26-32
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• 2017

We studied the effects of dip treatments of natural extracts (Camellia sinensis, Angelica gigas, Astragalus membranaceus, Glycyrrhiza uralensis: 1% extract) on the quality of peeled 'Daebo' chestnut. After dipping treatment peeled chestnuts were vacuum packaged with $75{\mu}m$ PE+Nylon 7-layer film then stored in $4^{\circ}C$ incubator for 35days. The quality changes including the surface color and off-odor were evaluated for storage days. The natural extracts dipping treatment on peeled chestnut was effective on skin browning inhibition. Especially, Astragalus membranaceus and Glycyrrhiza uralensis extract treatments was significantly effective in reducing surface browning. The color change (${\Delta}E$) of peeled chestnut was the highest (5.6) in control, whereas that was the lowest (3.4) under Astragalus membranaceus extract treatment at 35 storage day. The natural exteacts treatments had no effect on the weight, the mositure loss rate, the firmness and the soluble solid contencts of peeled chestnuts during storage after treatment. The decaying rate of peeled chestnut was 9.0% in control, 9.0% in Camellia sinensis extract treatment, 9.0% in Angelica gigas extract treatment, 21.0% in Astragalus membranaceus extract treatment, and 7.0% Glycyrrhiza uralensis extract treatment at 35 storage day. Sensory evaluation revealed significant differences in off-odor and color attributes. After all, the score of overall sensory evaluation was the lowest in Astragalus membranaceus extract treatment and the highest in Glycyrrhiza uralensis extract treatment. As the results, the natural extract treatments, especially with vacuum packing after Glycyrrhiza uralensis extract treatment, extended the shelf-life of the peeled chestnut by inhibiting the surface browning.

• Journal of mucopolysaccharidosis and rare diseases
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• v.5 no.1
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• pp.8-11
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• 2021

Glutaric aciduria type 1 (GA1; OMIM #231670) is a rare autosomal recessive-inherited neurometabolic disorder caused by the deficiency of glutaryl-CoA dehydrogenase (GCDH), which is encoded by the GCDH gene. It results in the accumulation of glutaric acid (GA), 3-hydroxyglutaric acid (3-OH-GA), glutaconic acid, and glutarylcarnitine (C5DC). These metabolites are considered to damage the striatum through an excitotoxic mechanism. The treatments of GA1 known to date are metabolic maintenance treatment based on a low-lysine diet and emergency treatment during acute illness. However, treatment after the onset of neurological symptoms has limited effectiveness and is associated with poor outcomes, and the effect of treatment and disease course after treatment are not good. After the implementation of newborn screening, the incidence of acute encephalopathic crisis fell to 10%-20% with early diagnosis, preventative dietary management, and aggressive medical intervention during acute episodes. Recently, several cohort studies have been published on the natural course and treatment of GA1 patients. This mini review will cover the clinical symptoms, natural history, and treatment of GA1 through a literature review.

• Journal of Environmental Health Sciences
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• v.20 no.3
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• pp.85-95
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• 1994

This study was performed to find out the detoxication effects of natural products Thuja orientalis, leaf of acorn, aloe ferox mill, pine cone, pine root, lonicerae flos on nickel toxicity. The experimental rats were divided into 3 groups such as nickel alone treatment group, natural products treatment groups before and after nickel treatments. Each group was administered with difference dose of nickel such as 6.6 mg/kg/day, 13.2 mg/kg/day, 19.8 mg/kg/day respectively, for 14 days. Natural products were administered by 400 mg per kilogram body weight rat per day. The weight change of rats, nickel concentration in organs, survival rate of rats, morphology of organs were observed in the experimental groups. The results obtained by the experiment were as follows: 1. The rats in the group treated with natural products had no effect on the weight gain. 2. The mean survival rate of rats in the nickel alone treatment group was 72.5% (6.6 mg/kg/day), 68.9% (13.2 mg/kg/day), 60.2% (19.8 mg/kg/day) respectively. 3. As for the amount of nickel accumulation in organs, it was the lowest amount treated with pine cone, pine root in that order. 4. When kidney and liver tissues were observed with as optical microscope, obvious change were visible in those tissues treated groups with Lonicerae and Aloe.

• Korean Journal of Rural Living Science
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• v.8 no.2
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• pp.83-91
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• 1997

In this study, the dyeability of natural dye extracted from chesnut shell was investigated in order to explore the using of discarded chesnut shell in natural dyeing. Dyeing experiments were done in various dyeing conditions which were different in dyeing temperature, concentration of dyebath, dyeing time, repitition of dyeing. pH of dyebath and mordant with 3 kinds of experimental fabrics silk, nylon and cotton. Color and color difference ($\Delta$ E) of every dyed fabrics were measured and color fastness to drycleaning, washing, perspiration and light were measured. The results were as follows ; 1. The dyebath became thicker with time and temperature of extraction and the characteristics of dyebath prepared chesnut shell 1g : distilled water 30㏄ after 3 hrs-boiling were 32,400ppm and 3.7pH. 2. The dyeabilities of silk and nylon fabrics were good and color difference was increased with dyeing temperature, concentration of dyebath, dyeing time, number of repitition and acidity of dyebath. But the dyeability of cotton was very poor compared to silk and nylon. 3. Without the treatment of mordant, the dyeability of silk was little lower than that of nylon, but after the treatment of mordants it became higher than nylon. After the treatment of mordant Cu and Fe, the dyeability of cotton was increased although the natural dye from the chesnut shell was hardly absorved in cotton without mordant. 4. On the whole, the colorfastness of dyed silk and nylon were very good except the colorfastness to washing in silk and the colorfastness to light in nylon.

• Archives of Pharmacal Research
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• v.21 no.4
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• pp.374-377
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• 1998

The freeze-dried powder of Lumbricus rubellus earthworm was administered orally to rats and its fibrinolytic and antithrombotic effects were investigated. The fibrinolytic activity of plasma was determined by measuring the plasmin activity of the euglobulin fraction and was increased to two-folds of the control at a dose of 0.5 g/kg/day and five times with 1 g/kg/day after 4-day administration. The antithrombotic effect was studied in an arterio-venous shunt model of rats. The thrombus weight decreased significantly from 43.2 mg to 32.4 mg at a dose of 0.5 g/kg/day after 8-day treatment. The level of fibrinogen/fibrin degradation product (FDP) in serum was elevated in a dose-dependent manner during the treatment period. On the 8th day after administration, the FDP value was increased to 7.7 $\mu\textrm{g}$g/ml compared with the control value of 3.3 $\mu\textrm{g}$g/ml. These results support that earthworm powder is valuable for the prevention and/or treatment of thrombotic conditions.

• Journal of Acupuncture Research
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• v.39 no.4
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• pp.239-248
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• 2022

To determine the effect of Korean medicine treatment of avascular necrosis of the femoral head (ANFH) this study reviewed both single ingredients and bioactive compounds in the treatment of ANFH in a rat model. Literature was retrieved from PubMed and Google Scholar using the keywords "femur head necrosis," "natural extract," and "rat." The data from studies analyzed included: rats' characteristics, development methods of ANFH, natural extracts administration, observation methods, and outcome indicators. Two independent researchers screened all articles retrieved and 26 studies were chosen. The most used rat species was the Sprague Dawley rat (76.9%). To induce ANFH, steroid injections (46.2%), and oral gavage (53.8%) were typically used. Studies focused mainly on factors affecting bone formation (65.3%), and apoptosis (53.8%). Research on ANFH focused on using traditional natural substances mentioned in classical literature to confirm its effectiveness against anti-inflammation, osteogenesis, and cell death. ANFH has a diverse etiology, therefore research models such as genetic analysis of human-derived samples from ANFH patients may shed more light on the condition. Moreover, research into herbal medicines and pharmacoacupuncture treatment of ANFH should precede.

• Journal of Nutrition and Health
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• v.36 no.2
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• pp.101-108
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• 2003

Antiplatelet aggregation, anticoagulant and lipid-lowering drugs are clinically widely used for secondary preventive purpose in the cardiovascular patients, but there is no primary preventive agents to prevent these diseases. With the aim of developing effective primary agents for cardiovascular diseases, we tried to formulate an optimized mixture of natural plants extract containing Theae sinensis, Camelliae sinensis, Vitis vinifera, Gingko folium and curcuminoids from Curcuma longa and to evaluate its anti-thrombotic and anti-hypercholesterolemic effects in vivo. The inhibitory effect of curcuminoids on vascular smooth muscle cell proliferation and migration were also investigated in vitro. in the animal experiments treated with hyperlipidemic diet, oral treatment of curcuminoids and natural plants extracts mixture (100 mg/kg) into male Sprague Dawley rats for 7 week simultaneously inhibited platelet aggregation as well as improved lipid profile in the blood. Compared to control group, both of curcuminoids-treated and mixture-treated groups revealed significantly decrease of total cholesterol (24.4%, 28.6%), free cholesterol (25.1%, 24.0%), cholesterol ester (14.6%, 29.0%), LDL-cholesterol (27.0%, 32.0%) and triglyceride (15.0%, 31.0%), respectively. However, both groups showed increase of HDL-cholesterol (46.6% and 51.5%) . In particular, atherogenic index of curcuminoids and mixture treatment group was significantly decreased to 47.0% and 56.0%, respectively. Furthermore, oral treatment of curcuminoids and mixture significantly inhibited collagen-induced platelet aggregation (21.1% and 29.1%, respectively), compared to control group. The anti-thrombotic values of mixture was almost similar to that of aspirin treatment (100 mg/kg) group. These results suggest that the oral treatment of curcuminoids-based natural plant extract mixture improved cardiovascular conditions in hyperlipidemic rats.