• Journal of People, Plants, and Environment
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• v.22 no.5
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• pp.455-466
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• 2019

This study was carried out to analyze trends in floral products and services through the big data analysis of various social networking services (SNSs) and then to provide objective marketing directions for the floricultural industry. To analyze the big data of SNSs, we used four analytical methods: Cotton Trend (Social Matrix), Naver Big Data Lab, Instagram Big Data Analysis, and YouTube Big Data Analysis. The results of the big data analysis showed that SNS users paid positive attention to flower one-day classes that can satisfy their needs for direct experiences. Consumers of floral products and services had their favorite designs in mind and purchased floral products very actively. The demand for flower items such as bouquets, wreaths, flower baskets, large bouquets, orchids, flower boxes, wedding bouquets, and potted plants was very high, and cut flowers such as roses, tulips, and freesia were most popular as of June 1, 2019. By gender of consumers, females (68%) purchased more flower products through SNSs than males (32%). Consumers preferred mobile devices (90%) for online access compared to personal computers (PCs; 10%) and frequently searched flower-related words from February to May for the past three years from 2016 to 2018. In the aspect of design, they preferred natural style to formal style. In conclusion, future marketing activities in the floricultural industry need to be focused on social networks based on the results of big data analysis of popular SNSs. Florists need to provide consumers with the floricultural products and services that meet the trends and to blend them with their own sensitivity. It is also needed to select SNS media suitable for each gender and age group and to apply effective marketing methods to each target.

• Journal of the Korea Society of Computer and Information
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• v.14 no.1
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• pp.1-8
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• 2009

Learning user's preference is a key issue in intelligent system such as personalized service. The study on user preference model has adapted simple user preference model, which determines a set of preferred keywords or topic, and weights to each target. In this paper, we recommend multi-perspective user preference model that factors sentiment information in the model. Based on the topicality and sentimental information processed using natural language processing techniques, it learns a user's preference. To handle timc-variant nature of user preference, user preference is calculated by session, short-term and long term. User evaluation is used to validate the effect of user preference teaming and it shows 86.52%, 86.28%, 87.22% of accuracy for topic interest, keyword interest, and keyword favorableness.

• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.788-796
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• 2023

Canine parvovirus type 2 (CPV-2) has high morbidity and mortality rates in canines. Nonstructural protein 1 (NS1) of CPV-2 has endonuclease activity, initiates viral DNA replication, and is highly conserved. Thus, it is a promising target for antiviral inhibitor development. We overexpressed a 41.9 kDa active recombinant endonuclease in Escherichia coli and designed a nicking assay using carboxyfluorescein and quencher-linked ssDNA as substrates. The optimal temperature and pH of the endonuclease were 37℃ and pH 7, respectively. Curcumin, bisdemethoxycurcumin, demethoxycurcumin, linoleic acid, tannic acid, and α-tocopherol inhibited CPV-2 NS1 endonuclease with IC₅₀ values of 0.29 to 8.03 µM. The extracted turmeric, yerba mate, and sesame cake suppressed CPV-2 NS1 endonuclease with IC₅₀ values of 1.48, 7.09, and 52.67 ㎍/ml, respectively. The binding affinity between curcumin, the strongest inhibitor, and CPV-2 NS1 endonuclease by molecular docking was -6.4 kcal/mol. Curcumin inhibited CPV-2 NS1 endonuclease via numerous hydrophobic interactions and two hydrogen bonds with Lys97 and Pro111 in the allosteric site. These results suggest that adding curcuminoids, linoleic acid, tannic acid, α-tocopherol, extracted turmeric, sesame cake, and yerba to the diet could prevent CPV-2 infection.

• Molecules and Cells
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• v.46 no.4
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• pp.191-199
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• 2023

The Golgi apparatus modifies and transports secretory and membrane proteins. In some instances, the production of secretory and membrane proteins exceeds the capacity of the Golgi apparatus, including vesicle trafficking and the post-translational modification of macromolecules. These proteins are not modified or delivered appropriately due to the insufficiency in the Golgi function. These conditions disturb Golgi homeostasis and induce a cellular condition known as Golgi stress, causing cells to activate the 'Golgi stress response,' which is a homeostatic process to increase the capacity of the Golgi based on cellular requirements. Since the Golgi functions are diverse, several response pathways involving TFE3, HSP47, CREB3, proteoglycan, mucin, MAPK/ETS, and PERK regulate the capacity of each Golgi function separately. Understanding the Golgi stress response is crucial for revealing the mechanisms underlying Golgi dynamics and its effect on human health because many signaling molecules are related to diseases, ranging from viral infections to fatal neurodegenerative diseases. Therefore, it is valuable to summarize and investigate the mechanisms underlying Golgi stress response in disease pathogenesis, as they may contribute to developing novel therapeutic strategies. In this review, we investigate the perturbations and stress signaling of the Golgi, as well as the therapeutic potentials of new strategies for treating Golgi stress-associated diseases.

• Design & Manufacturing
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• v.16 no.2
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• pp.33-38
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• 2022

Currently, Korea is an aging society, and it is expected to enter a super-aging society in about 4 years. Accordingly, many X-ray technologies are being developed. In X-rays, 99% of X-rays are converted into heat energy and 1% into light energy (X-rays). 99% of the thermal energy raises the temperature of the anode and its surroundings, and the cooling system is an important factor as overheating can affect the deterioration of X-ray quality and shortened lifespan. There is a method of forced air cooling using natural convection. Therefore, in this study, when X-rays were taken 5 times, Flow analysis was performed on heat removal according to temperature rise and cooling time for the heat generated at the anode of the X-ray tube (input power 60kW, 75kW, 90kW). Based on one-shot, the most rapid temperature rise section increased by more than 57% to 0.03 seconds, A constant temperature rises from 0.03 seconds to 0.1 seconds, It is judged that the temperature rises by about 8.2% or more at one time. After one-shot cooling, the cooling drops sharply from about 60% to 0.03 seconds, It is judged that the temperature has cooled by more than 86% compared to the temperature before shooting. One-shot is cooled by more than 86% with cooling time after 0.1 seconds, As the input power of the anode increases, the cooling temperature gradually increases. Since the tungsten of the anode target inside the X-ray tube may be damaged by thermal shock caused by a rapid temperature rise, an improvement method for removing thermal energy is required when using a high-input power supply.

• Journal of Digestive Cancer Research
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• v.4 no.2
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• pp.64-76
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• 2016

The step-wise process of colorectal carcinogenesis from aberrant crypt foci, adenoma to adenocarcinoma, is relatively suitable for chemopreventive intervention. Accumulated evidences have revealed that maintaining an undifferentiated state (stemness), inflammation, and oxidative stress play important roles in this colon carcinogenesis process. However, appropriate molecular targets that are applicable to chemopreventive intervention regarding those three factors are still unclear. In this review, we summarized appropriate molecular targets by identification and validation of the prospective targets from a comprehensive overview of data that showed colon cancer preventive effects in clinical trials, epidemiological studies and basic research. We first selected a study that used aspirin, statins and metformin from FDA approved drugs, and epigallocatechin-gallate and curcumin from natural compounds as potential chemopreventive agents against colon cancer because these agents are considered to be promising chemopreventive agents. Experimental and observational data revealed that there are common target molecules in these potential chemopreventive agents: T-cell factor/lymphoid enhancer factor (TCF/LEF), nuclear factor-&B (NF-κB) and nuclear factor-erythroid 2-related factor 2(NRF2). Moreover, these targets, TCF/LEF, NF-κB and NRF2, have been also indicated to suppress maintenance of the undifferentiated state, inflammation and oxidative stress, respectively. In the near future, novel promising candidate agents for colon cancer chemoprevention could be identified by integral evaluation of their effects on these three transcriptional activities.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.26 no.5
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• pp.33-46
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• 2023

In this study, carbon storage in the aboveground biomass, litter layer, and soil layer was calculated for abandoned mining restoration areas to determine the level of carbon storage after the restoration project through comparison with the ecological reference. Five survey sites were selected for each abandoned mining restoration area in Boryeong-si, Chungcheongnam-do, and the ecological reference that can be a goal and model for the restoration project. The carbon storage in the restoration area was 0~21.3Mg C ha^-1, the deciduous layer 3.3~6.0Mg C ha^-1, and the soil layer(0-30cm) 8.3~35.1Mg C ha^-1, showing a significant difference in carbon storage by target site. The total carbon storage was between 6.1 and 35.3% of the ecological reference, with restoration area ranging from 14.0 to 62.4 Mg C ha^-1. The total carbon storage in the restoration area and the ecological reference differed the most in the aboveground biomass and was less than 12%. Based on these results, forest restoration area need to improve the carbon storage of forests through continuous management and monitoring so trees can grow and restore productivity in the early stages of the restoration project. The results of this study can be used as primary data for preparing future forest restoration indicators by identifying the storage of abandoned mining restoration areas.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.55-55
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• 2023

With the COVID-19 pandemic, there is increasing interest in anti-obesity strategies. According to the National Statistical Office, the obesity rate in Korea was 38.3% in 2020 and 37.1% in 2021. Obesity is a risk factor for several severe diseases, including stroke, heart disease, type 2 diabetes, and certain types of cancer. Pinus rigida × Pinus taeda is a hybrid of Pinus rigida Mill and Pinus taeda Linn, and its cones are considered a by-product. Although previous studies have investigated their pharmacological effects on antioxidant activity and protection against oxidative DNA damage, few researchers have explored their potential as functional natural materials. Therefore, we evaluated the anti-obesity effects of the cone of ethyl acetate fraction of P. rigida × P. taeda (ERT), specifically its ability to inhibit lipid accumulation. Our analysis showed that ERT contains phytochemicals (catechin and caffeic acid) which are known to improve immune function and inhibit cell damage. ERT inhibited lipid droplet accumulation at the cellular levels through Oil Red O staining. Furthermore, ERT suppressed the expression of adipogenic transcription factors (PPARγ and CEBP/α) as well as downstream lipogenic target genes (FAS and SREBP-1) thereby inhibiting adipogenesis. ERT also down-regulated key adipogenic markers, including aP2α, while inducing the phosphorylation of AMPK. It has been reported that PPARγ and CEBP/α are expressed in the early stages of adipose differentiation, while SREBP-1 is expressed in the late stage. Therefore, our findings suggest that ERT activates AMPK signaling pathways, which inhibits adipogenic transcription factors (PPARγ, C/EBPα, and SREBP1) and lipogenic genes (FAS and aP2α), thereby blocking lipid accumulation and preventing obesity and related disorders. ERT showed potential as a new resource for developing a functional material for anti-obesity agents.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.56-56
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• 2023

The cone of Pinus rigida × Pinus taeda (PRT), a plant in the Pinaceae family, has long been used in traditional medicine to treat hemostasis, bruises, and burns. Previous research has shown that regulating oxidation-reduction reactions in reactive oxygen species can help inhibit melanogenesis, the process of melanin synthesis, which is a common target for addressing hyperpigmentation. Inhibiting tyrosinase is also known to be effective in this regard. Based on these findings, we conducted an investigation into the inhibitory effect of the ethyl acetate fraction of PRT (ERT) on melanogenesis in B16 F10 cells. We know that the expression levels of melanin biosynthesis-related proteins, including tyrosinase, TRP-1, and TRP-2, are regulated by MITF (microphthalmia-associated transcription factor) and cAMP, with cAMP affecting the activity of protein kinase A (PKA). PKA can reduce melanogenesis, and CREB reduces the phosphorylation of melanin-producing enzymes. In addition, the MAPK signaling pathway, composed of ERK, JNK, p38, and other factors, is also known to play a role in the inhibition of melanogenesis in melanocytes. Our immunoblotting results showed that ERT inhibited the expression of melanin production-related proteins (tyrosinase, TRP-1, TRP-2, and MITF) that were significantly increased by a-MSH treatment to promote melanin production. Furthermore, the phosphorylation levels of factors related to cAMP/PKA/CREB and MAPK signaling pathways were significantly reduced without affecting the total form. In conclusion, we believe that treatment with ERT can inhibit melanin synthesis by modulating the phosphorylation of cAMP/PKA/CREB and MAPK signaling pathways at the cellular level. These findings suggest the potential of ERT as a raw material for functional cosmetics and pharmaceuticals, thanks to its antioxidant activity and ability to inhibit melanogenesis. We thought that these findings of ERT as a natural plant resource will inspire further research and development in this area.

• Journal of Integrative Natural Science
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• v.16 no.3
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• pp.81-95
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• 2023

Colorectal cancer is one of the most common types of cancer worldwide, ranking third after lung and breast cancer in terms of global prevalence. With an expected 1.93 million new cases and 935,000 deaths in 2020, it is more prevalent in males than in women. Evidence has shown that during the later stages of colon cancer, STAT1 promotes tumor progression by promoting cell survival and resistance to chemotherapy. Recent studies have shown that inhibiting STAT1 pathway leads to a reduction in tumor cell proliferation and growth, and can also promote apoptosis in colon cancer cells. One of the recent approaches in the field of drug discovery is drug repurposing. In drug repurposing approach we have virtually screened FDA database against STAT1 protein and their interactions have been studied through Molecular docking. Cross docking was performed with the top 10 compounds to be more specific with STAT1 comparing the affinity with STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. The drugs that showed higher affinity were subjected to Conceptual - Density functional theory. Besides, the Molecular dynamic simulation was also carried out for the selected leads. We also validated in-vitro against colon cancer cell lines. The results showed mainly Acetyldigitoxin has shown better binding to the target. From this study, we can predict that the drug Acetyldigitoxin has shown noticeable inhibitory efficiency against STAT1, which in turn can also lead to the reduction of tumor cell growth in colon cancer.