• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.196-205
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• 2015

A Sulfolobus-E. coli shuttle vector for an efficient expression of the target gene in S. acidocaldarius strain was constructed. The plasmid-based vector pSM21 and its derivative pSM21N were generated based on the pUC18 and Sulfolobus cryptic plasmid pRN1. They carried the S. solfataricus P2 pyrEF gene for the selection marker, a multiple cloning site (MCS) with C-terminal histidine tag, and a constitutive promoter of the S. acidocaldarius gdhA gene for strong expression of the target gene, as well as the pBR322 origin and ampicillin-resistant gene for E. coli propagation. The advantage of pSM21 over other Sulfolobus shuttle vectors is that it contains a MCS and a histidine tag for the simple and easy cloning of a target gene as well as one-step purification by histidine affinity chromatography. For successful expression of the foreign genes, two genes from archaeal origins (PH0193 and Ta0298) were cloned into pSM21N and the functional expression was examined by enzyme activity assay. The recombinant PH0193 was successfully expressed under the control of the gdhA promoter and purified from the cultures by His-tag affinity chromatography. The yield was approximately 1 mg of protein per liter of cultures. The enzyme activity measurements of PH0913 and Ta0298 revealed that both proteins were expressed as an active form in S. acidocaldarius. These results indicate that the pSM21N shuttle vector can be used for the functional expression of foreign archaeal genes that form insoluble aggregates in the E. coli system.

• Molecules and Cells
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• v.39 no.4
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• pp.299-309
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• 2016

We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.6
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• pp.1-9
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• 2017

This study investigates the environmental factors of endemic species in Korea in order to understand their ecological characteristics, and to investigate the target species of their natural habitats to find similar sites. The purpose is to restore and follow suitable growth methods for the appropriate highway facility of target species to establish a management system via monitoring. This study endeavors to restore the target species near highway facilities on the basis of monitoring data and restore sites have similar natural characteristics of the target species. After restoring the target species, a restoration strategy and management plan will be established for breeding and continuation. The restoration strategy and management plan of the target species is divided into breeding, restoring, maintaining and monitoring plans. Specially management plans include several divisions such as soil, water, non-point pollution source reduction and naturalized plants. The results of this study can be used as reference materials for the restoration of endemic Korean plants in the future of highway routes, and for systematic management measures in habitats.

• ETRI Journal
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• v.36 no.5
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• pp.704-713
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• 2014

Most natural language processing tasks depend on the outputs of some other tasks. Thus, they involve other tasks as subtasks. The main problem of this type of pipelined model is that the optimality of the subtasks that are trained with their own data is not guaranteed in the final target task, since the subtasks are not optimized with respect to the target task. As a solution to this problem, this paper proposes a consolidation of subtasks for a target task ($CST^2$). In $CST^2$, all parameters of a target task and its subtasks are optimized to fulfill the objective of the target task. $CST^2$ finds such optimized parameters through a backpropagation algorithm. In experiments in which text chunking is a target task and part-of-speech tagging is its subtask, $CST^2$ outperforms a traditional pipelined text chunker. The experimental results prove the effectiveness of optimizing subtasks with respect to the target task.

• Journal of Korean Biological Nursing Science
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• v.4 no.2
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• pp.59-68
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• 2002

The purpose of this study was to determine the effect of exercise program on natural killer cell cytotoxic activity(NKCA) in breast cancer patients who had been radiation therapy after surgery. The subjects in the experimental group consisted of 11 breast cancer patients, while the subjects in the control group consisted of 15. Subjects in the experimental group participated in exercise program for 8 weeks. Exercise program consisted of shoulder stretching, arm weight training and treadmill walking exercise. They started to exercise on treadmill for 20 minutes per day, 3 times a week at 40% of maximum heart rate, and increased intensity and duration of exercise so that they were running 30 minutes/day at 60% of maximum heart rate from the 3rd week to the 8th week. Natural killer cell cytotoxic activity were determined before and after the exercise program. For measuring the natural killer cell cytotoxic activity, 8ml to 10ml blood was collected from the subjects. Mononuclear cell was isolated by centrifuge of the blood and cultured by putting $Cr^{51}$, and reacted with target cell, K562 cell. Baseline demographic and medical data were compared between groups with the Fisher's exact test and Mann-Whitney U test. For effects of the exercise program, repeated measures ANOVA was used. The result was as follows; Natural killer cell cytotoxic activity(NKCA) in experimental group comparing with control group significantly increased after the exercise program in case of effector cell : target cell ratio is 100 : 1(p<0.05). The above result suggest that the exercise program for breast cancer patients undergoing radiation therapy after breast surgery may increase the natural killer cell cytotoxic activity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.12
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• pp.7302-7308
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• 2014

Detecting target objects robustly in natural environments is a difficult problem in the computer vision and image processing areas. This paper suggests a method of robustly detecting target objects in the environments where reflection exists. The suggested algorithm first captures scenes with a stereo camera and extracts the line and corner features representing the target objects. This method then eliminates the reflected features among the extracted ones using a homographic transform. Subsequently, the method robustly detects the target objects by clustering only real features. The experimental results showed that the suggested algorithm effectively detects the target objects in reflection environments rather than existing algorithms.

• Proceedings of the Korean Biophysical Society Conference
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• 1999.06a
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• pp.13-13
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• 1999

"Two-component" phosphorelay signal transduction systems constitute a potential target for antibacterial and antifungal agents, since they are found exclusively in prokaryotes and lower eukaryotes (yeast, fungi, slime mold, and plants) but not in mammalian organisms.(omitted)

• Korean Journal of Agricultural Science
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• v.38 no.1
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• pp.109-113
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• 2011

The purpose of this study was to examine the potentiality of urban forest roads as an environment for enhancing physical fitness. Six male university students participated in the study as subjects. The subjects walked on an urban forest road for 30 minutes. As a control experiment, they also walked on a national park trail for 30 minutes. Subjects' heart rates were monitored during the walks to calculate the ratio of the average time their heart rates were within the target range (from 60% to 80% of the maximal heart rate) for Enhancing Physical Fitness. After the walks, images of the spaces were analyzed using the semantic differential (SD) method. During the walk on the urban forest road, subjects' heart rates were within the target range 63.3% of the time, and lower than the target range 36.7% of the time. During the control experiment on the national park trail, subjects' heart rates were within the target range only 23.3% of the time, and higher than the target range 76.7% of the time. From the spatial perception evaluation using the SD method, subjects' comfortable and natural feelings when they were on the national park trail were significantly greater than when they were on the urban forest trail, but there were no differences in terms of other SD descriptors, such as friendliness and likeability. The results of our study indicate that the urban forest road provides a good environment for walking to enhance physical fitness. Although not as close to nature as national park trails, urban forest roads offer similar natural environments and have a high potentiality for serving as leisure spaces for urban residents who seek physical activities.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.47 no.5
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• pp.62-68
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• 2010

This study is to identify target locations with low false alarms on thermal infrared images obtained from natural environment. The proposed method is different from the previous researches because it uses morphology filters for Gabor response images instead of an intensity image in initial detection stage. This method does not need precise extracting a target silhouette to distinguish true targets or clutters. It comprises three distinct stages. First, morphological operations and adaptive thresholding are applied to the summation image of four Gabor responses of an input image to find out salient regions. The locations of extracted regions can be classified into targets or clutters. Second, local texture features are computed from salient regions of an input image. Finally, the local texture features are compared with the training data to distinguish between true targets and clutters. The multi-layer perceptron having three layers is used as a classifier. The performance of the proposed method is proved by using natural infrared images. Therefore it can be applied to real automatic target detection systems.

• Journal of the Korean housing association
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• v.17 no.3
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• pp.99-109
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• 2006

The purpose of this study was to analyze the characteristic of each view in an apartment unit in terms of the quality. This study was accomplished by two research methods; the field survey on 181 apartment complexes in Daegu, and the preference evaluation on 24 cases sampled in the sense of its location, i.e. inside or outside of a complex. In the field survey, view targets were categorized into various items such as a building, a road, a mountain, a tree, another apartment, the sky, and so on, and the characteristics of the distance from a viewer to view targets and view of the level in an apartment were analyzed. In the preference evaluation, 24 cases, each one has one of the follows; the natural view, the artificial view, and the combined view of the both, were chosen and those were evaluated by 167 interviewees. As a result, the conclusions from the study were drawn as follows; 1) The view, the combination of buildings, roads, mountains, trees, apartments, and the sky, is usually seen in an apartment unit. 2) The type of view target is more significant to a person than its location. 3) The natural and combined view are preferred to the artificial one. 4) In the case of the same outside view, a person likes the open view more than the somewhat covered one.