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Rapid detection of Theileria sergenti by the polymerase chain reaction in Korean cattle (중합효소연쇄반응을 이용한 한우에 감염된 Theileria sergenti의 신속한 검출)

  • Chae, Joon-seok;Lee, Joo-mook;Kwon, Oh-deog;Park, Jin-ho;Chae, Keon-sang
    • Korean Journal of Veterinary Research
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    • v.36 no.1
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    • pp.195-207
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    • 1996
  • To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from erythrocytes of Korean cattle, The previous studies on the probe of T sergenti had resulted in two probes as KTS1 and KTS3 DNA fragment. Nucleotide sequence of both ends of the KTS1 and KST3 were determined in order to design primers for polymerase chain reaction. A pair of an uper primer(5'-CCTCTTGAAGTCATCCATGT-3'; nucleotide position 48) and a lower primer(5'-CACTGAGCTG GAAAGAGCTA-3'; nucleotide position 156) in pKTS1 were synthesized. The anticipated PCR product was 128bp in length. To examine the sensitivity of the PCR, KTS1 DNA and purified T sergenti DNA were serially diluted by tenfolds with distilled water. The primers were sensitive enough to detect 4ag of the authentic template DNA and 4fg of the purified T sergenti DNA by PCR. Furthermore, when the blood was serially diluted by two-folds with 0.9% saline, the pair could detect up to 0.00029%(about 164 parasites in $10{\mu}l$ of blood) of T sergenti infection in bovine erythrocytes by PCR. In a comparison of microscopic and PCR detection of T sergenti in the same samples from Chonbuk area, 47 and 51 out of 70 sample(67.1%) were positive by the former and by the latter method, respectively.

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Vibration Mode and Durability Characteristics of Automotive IDS using Rotary Swaging Process for Incremental Forming (로터리 스웨이징 공정의 점진성형에 의한 중공 드라이브샤프트의 진동모드 및 내구특성)

  • Lim Seong-Joo;Lee Nak-Kyu;Lee Chi-Hwan
    • Transactions of the Korean Society of Automotive Engineers
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    • v.13 no.5
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    • pp.127-133
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    • 2005
  • Rotary swaging is one of the incremental forming process which is a chipless process using the reduction of cross-sections of bars, tubes and wires. The TDS(Tube Drive Shaft) of monobloc used in automotive has been developed by the rotary swaging process. The mechanical characteristics of swaged parts such as the hardness, thickness and roughness are also estimated to conduct experimental analyses of rotary swaging process with the materials of 34Mn5 Furthermore the change in the vibration mode of TDS due to design parameters, which are the tube length, diameter and thickness, has been investigated and analysed. The weight of the TDS product is smaller by about $12.8\%$ than that of SDS with the same performance. It could be evidently found that the TDS is designed to be much lighter than SDS (Solid Drive Shaft). This advantage might give some possibility to improve the NVH (Noise-Vibration-Harshness) characteristics. A maximum torque and a total number of torsional repetitions for the TDS is checked and measured to know the torsional intensity and fatigue strength through the static torsion test and torsional durability test, respectively. A total number of the torsional repetitions up to the fracture for the TDS is greater than 250,000 times.

Study on Practical Evaluation for Sustainability in Textile and Fashion Companies (섬유·패션기업의 지속가능성 실천 평가 연구)

  • Syn, Hye-Young;Geum, Key-Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.38 no.2
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    • pp.137-146
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    • 2014
  • The practice of sustainability is an important subject to integrate into the management, product planning, manufacturing by textile and fashion companies as well as consumption by end-users. This study shows an evaluation scale designed to assess the practical cases in order to suggest efficient and systematic methods to fulfill the practice of sustainability in textile and fashion companies. First, companies should practice environmental sustainability to use eco-friendly material and cleaner production, protect the environment, and save natural resources. Second, social sustainability is required to fulfill social responsibility and ethical needs. Third, companies should perform various innovative activities, transparency in management, fair competition and economic contribution in the local community in order to maintain economic sustainability to survive in industry. Finally, cultural sustainability should be fulfilled by textile and fashion companies as part of the intellectual-cultural industry in a way that increases the importance of ethnic and cultural diversity. Textile and fashion companies should fulfill four environmental, social, and economic cultural sustainable subjects in a balanced method to accomplish sustainability. The concerns and practices of environmental sustainability are comparatively highly rated due to the analysis of the evaluation scale. However, cultural sustainability (a need of the times) is poorly rated and needs more attention. Therefore, the evaluation scale can be used as a standard tool to fulfill the sustainability of companies and brands from the viewpoint of improving poor and insufficiently sustainable items as well as balancing sustainability management.

A Relationship Between Fashion Image Types and Design Factors(I) (패션 이미지 유형(類型)과 디자인 요소(要素)와의 관계(關係)(I))

  • Kim, Il-Boon;Yoo, Tai-Soon
    • Journal of Fashion Business
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    • v.1 no.3
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    • pp.19-34
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    • 1997
  • The role of fashion image in modern society is being diversified from product planning to consumer satisfaction. In this research, after surveying female college students, the diversified modern fashion image was patterned. On the analogy of the relationship between these patterned image and design factors, the following conclusion was obtained. 1. The most popular styles are jacket and pants suits, three-piece outfits, tight mini-skirts and X-silhouette. Little decoration is used in general. simplicity is shown as trend by utility fabrics in single color-black, brown, beige and blue-with no pattern. Various materials such as wool, cotton, wool or cotton blended with other materials, silk, satin, knit, lace, vinyl and leather are used. 2. Fashion image is divided into four factors; factors that show fad, dignity, activity and simplicity. 3. Factors that show fad; style of tops, material of bottoms and length of bottoms reflect fad. blousons, three-piece outfits, pants rather than skirts, X-silhouette and mini-skirts are prevailing. Vinyl, leather, knit, lace and satin are the materials well used. 4. Factors that show dignity; Style and material of tops, the way of top and bottom pieces are matched and length of bottom or pieces reveal the characteristics. Jackets, blouses, half-coats and one-piece outfits rather than two-pieces are popular. Tight skirts in natural or midi length or pleated skirts are common. Wool, wool blended with other materials, leather and X-silhouette are design factors that reveal dignity well. 5. Design factors that show active image; Straight pants, three-piece outfits, T-shirts, shirt -blouses, vests, and blausons are popular. Knit and lace are used for tops, leather and vinyl are used for bottom pieces and cotton is widely used. Y- or H-silhouette are used to show active image.

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Development of Solid/Liquid Separation Technique for Krill (Eupausia superba) (남극 크릴새우의 고액분리 기술개발)

  • Oh, I.H.;Jang, C.H.;Kim, W.G.;Yang, S.Y.
    • Journal of Animal Environmental Science
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    • v.17 no.1
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    • pp.33-38
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    • 2011
  • Economic development involves increase in life expectancy as well as human health care. Consequently, demand for fish meal and fish oil is rapidly growing. In particular, Krill (Eupausia superba) oil product is in high demand due to its rich unsaturated-fatty acid, and thus stable supplies are necessary in the krill oil market. It is required for captured krills to be immediately frozen and stored during ship transport, since proteins of the krill are quickly denatured in natural temperature condition. However, the transportation cost has been sharply increased, which encourages researchers to involve in studies for development of efficient oil extraction process. In this study, a solid/liquid separation technique on boat for the krill oil was developed through triple separation tests using only a separator or using either brush or crusher prior to the separator. The separation tests revealed that the efficiency were 46.2, 60.2 and 60.4 % by the separator, combination with brush, and combination with crusher, respectively. In addition, it was found that byproduct, extracted cake, derived from the separation process could be used as a feed stuff. These results suggest that smashing using the brush or crusher prior to the separator is more efficient than using only the separator.

Influence of Current Density Application Time on the Corrosion Damage of Offshore Wind Steel Substructure in Galvanostatic Corrosion Experiment (해상풍력 하부 구조물용 강재의 정전류 부식 시험 시 전류밀도 인가 시간이 부식손상에 미치는 영향)

  • Lee, Jung-Hyung;Park, Jae-Cheul;Han, Min-Su;Jang, Seok-Ki;Kim, Seong-Jong
    • Journal of the Korean institute of surface engineering
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    • v.49 no.5
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    • pp.431-438
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    • 2016
  • This research investigated the relationship between the corrosion damage characteristics of offshore wind steel substructure and the time of current density application by electrochemical accelerated short-term corrosion test. The galvanostatic corrosion was conducted on the steel specimens in natural seawater with a constant current density ranging from $1mA/cm^2$ to $200mA/cm^2$ for 1 ~ 180 min. Macro and micro observation was carried out on the surface of the corrosion damaged area using SEM and 3-dimensional analysis microscope. The weight loss of the specimens before and after was calculated as the difference between the initial weight prior to corrosion and weight after removal of the corrosion product. It was shown that during galvanostaic corrosion process, the corrosion behavior could be characterized by the onset of pitting corrosion in the early stage and the uniform corrosion in the late stage, showing damage development in the depth direction with the time of current application. The result of the 3D analysis revealed that both damage depth and surface roughness increased with increasing time of current application. The weight loss curves with time showed that a coefficient of determination ($R^2$) was relatively high for the relationship between the time of current application and weight loss. As a result, the degree of corrosion can be controlled by simply varying the time of current application.

Effects of Drying Temperature on Antioxidant Activities of Tomato Powder and Storage Stability of Pork Patties

  • Kim, Hyeong Sang;Chin, Koo Bok
    • Food Science of Animal Resources
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    • v.36 no.1
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    • pp.51-60
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    • 2016
  • This study was performed to evaluate the antioxidant activity of oven-dried tomato powder (OTP) as affected by drying temperature and the effect of OTP on the product quality of pork patties. Three OTP products were obtained by drying of fresh tomato at 60, 80 and 100℃ oven until constant weight was obtained. Total phenolic content of three kinds of OTPs ranged from 1.95 to 5.94 g/100 g. The highest amount of total phenolic compound was observed in OTP dried at 100℃. Antioxidant activity of three kinds of OTPs was measured by 1,1-diphenyl-2-pycrylhydrazyl (DPPH)-radical scavenging activity, iron chelating ability, reducing power and measurement of lipid peroxide in linoleic acid emulsion system. In all parameters, OTP at 100℃ showed the higher antioxidant activity than other temperatures (p<0.05). Based on the model study, the physicochemical properties, and antioxidant and antimicrobial activities of pork patties containing 1% OTP were measured. Redness of pork patties were increased with the addition of OTPs (p<0.05). Thiobarbituric acid reactive substances (TBARS) values of raw pork patties containing OTPs were lower than those of control (CTL) until 7 d of storage, regardless of drying temperatures (p<0.05). Peroxide values of pork patties made with OTP (1%) were lower than those of CTL until the end of storage time (p<0.05). However, no antimicrobial activities were observed among the treatments (p>0.05). Therefore, OTPs could be used as a natural antioxidant in meat products.

Effect of Solvents and Surfactants on the Whole-cell Bioassay for Screening Quorum Sensing Autoinducers Using the Recombinant Agrobacterium tumefaciens NTL4 Strain (재조합 Agrobacterium tumefaciens NTL4 균주를 이용한 quorum Sensing Autoinducer 검색에 용매와 계면활성제가 미치는 영향)

  • Koh, Kyong-Pyo;Kim, Yeon-Hee;Kim, Jung Sun;Park, Sunghoon
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.4
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    • pp.260-267
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    • 2006
  • The Liquid Culture Assay using a recombinant Agrobacterium tumefaciens strain has been developed as a means for quorum sensing autoinducer screening. However, the low aqueous solubility of marine natural product extracts used as potential autoinducers has been a hindrance in the screening process. Although the addition of organic solvents and/or surfactants could increase aqueous solubility, errors in data interpretation including false positive results could be a serious problem. Therefore, determining the best possible solvent and surfactant at the optimum concentration is crucial. Evaluating methanol, ethanol, 1-propanol, DMSO and DMF at concentration ranges of 0~10% revealed < 2% methanol to be most favorable when tested for ${\beta}$-gal activity and growth inhibition of the recombinant A. tumefaciens strain. On the other hand, among surfactants tested, Triton X-100 was similarly effective in increasing the delivery of autoinducers for activity at less than 0.05% concentration.

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Characteristics of Preparative Liquid Chromatography (제조용 액체 크로마토그래피의 특성)

  • Row, Kyung-Ho;Jin, Yin-Zhe
    • KSBB Journal
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    • v.20 no.3
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    • pp.149-163
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    • 2005
  • Recently preparative liquid chromatography (PLC) has been used more frequently to separate drugs and natural substances. This modern separation methodologies require reliable tools that perform on a high level in terms of efficiency and reproducibility. However, large-scale PLC easily tends to reduce the yield and purity of the product. To promote the separation efficiency of PLC, we need to properly understand the controlling effects of the process, which may enable to predict the process and to improve the design and operation of PLC. Progress in computer technology allows the use of sophisticated models, provided their parameters can be measured. Some hardwares as well as softwares for PLC were already commercially available. In this work, the separation characteristic of PLC will be reviewed and compared on both the software and the hardware.

Eunsa Memorial Science Museum and Colonial Science Technology (은사기념과학관(恩賜記念科學館)과 식민지 과학기술)

  • Jung In-Kyung
    • Journal of Science and Technology Studies
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    • v.5 no.2 s.10
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    • pp.69-95
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    • 2005
  • Eunsa Memorial Science Museum is a political space to justify ruling colony. Japanese imperialism made use of science museum in ruling colony under the cloak of propagating science thoughts. The science museums made it natural to rule the inferior Joseon(Korea) by bring the concept of 'Great Science Empire' into relief. The exhibition, lecture, experiment and science movies propagated those colonial ruling ideology. This transplantation of the colonial science museumraised the following problems. First, the science museum was used as means for the propagation of political power. All the aspects of the architecture, exhibition, and operation of the colonial science museum propagated and supported the direction of political authority, and furthermore planted a rosy phantasm of 'Development' and 'Progression' into the colony. Second, The science technology of science museum was treated as 'Result' and 'Instrument'. Japanese imperialism denied that the science is a historical and cultural staple product; it instilled only the 'Instrumental Rationality' in the colony. Third, the science technology dealt in the colonial science museum was below the level. What they educated and set forth as domestic science was to cultivate the laborers people for the political power by internalizing modernistic discipline.

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