KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristics of Preparative Liquid Chromatography

제조용 액체 크로마토그래피의 특성

  • Row, Kyung-Ho (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Jin, Yin-Zhe (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University)
  • 노경호 (초정밀생물분리기술연구센터, 인하대학교 화학공학과) ;
  • 김은철 (초정밀생물분리기술연구센터, 인하대학교 화학공학과)
  • Published : 2005.06.01
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Abstract

Recently preparative liquid chromatography (PLC) has been used more frequently to separate drugs and natural substances. This modern separation methodologies require reliable tools that perform on a high level in terms of efficiency and reproducibility. However, large-scale PLC easily tends to reduce the yield and purity of the product. To promote the separation efficiency of PLC, we need to properly understand the controlling effects of the process, which may enable to predict the process and to improve the design and operation of PLC. Progress in computer technology allows the use of sophisticated models, provided their parameters can be measured. Some hardwares as well as softwares for PLC were already commercially available. In this work, the separation characteristic of PLC will be reviewed and compared on both the software and the hardware.

PLC는 약물과 천연물질의 분리에 많이 사용된다. 분석용으로부터 제조용으로의 scale-up에 따른 분리도가 낮아지는 것을 방지하여야 하며 또한 분리에 대한 효율성과 생산성을 고려하여야 한다. PLC의 최적조건을 선정하는 것은 많은 조업변수에 대한 고려를 해야 하는 힘든 작업이다. 소프트웨어를 이용한 분리 공정의 디자인, 최적화, 모사, 분석 등을 통하여 시간을 절약할 수 있으며 효율적으로 공정에 적용되게 한다. 하드웨어에 대한 기초 실험과 예비 생산을 통하여 이러한 공정에 대한 모사를 검증하고 생산량을 결정하고 분리 효율을 증가시킬 수 있다. 소프트웨어와 하드웨어의 특성, 종류, 응용을 예로 들어 설명함으로써 분리하고자 하는 물질에 대한 적합한 시스템을 선택하고 적용하여 분리공정을 생산화 할 수 있다. 크로마토그래피의 중요성이 증가함에 따라 고성능 및 사용하기 더욱 편리 한 소프트웨어와 하드웨어가 나타날 것이며 많은 영역의 분리 공정에서 필수적으로 이용될 것이다.

Keywords

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