• Archives of Pharmacal Research
• /
• v.26 no.2
• /
• pp.147-150
• /
• 2003

To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA strand-scission activity. Therefore, the ethyl acetate extracts of the dried heartwood of C. sappan was subjected to the bioassay-guided fractionation, which led to the isolation of a known compound, brazilin (1) as the active constituent. In addition, caesalpine J (2) was also isolated as an inactive constituent.

• Natural Product Sciences
• /
• v.3 no.1
• /
• pp.19-28
• /
• 1997

Constitutive cyclooxygenase (COX-I) is present in cells under physiological conditions, whereas inducible cyclooxygenase (COX-II) is induced by some cytokines, mitogens, and endotoxin presumably in pathological conditions such as inflammation. We have evaluated the inhibitory effects of solvent fractionated extracts of natural products on the activities of COX-I and COX-II. Oxygen uptake COX assay was performed, as a primary screening from the tissue extracts of bovine seminal vesicles (BSV), by monitoring the initial rate of oxygen uptake using an oxygen electrode. Additionally, we evaluated plant extracts for the inhibitory effects of COX-I (in HEL cells) and COX-II (in lipopolysaccharide activated J774A.1 macrophages) using thin layer chromatography of prostanoids produced from $^{14}C-labelled$ arachidonic acid (AA). The use of such models of COX-I and COX-II assay will lead to the identification of specific inhibitors of cyclooxygenases with presumably less side effects than present therapies. Inhibitory effects of 50 kinds of plant extracts on the COX-I and COX-II activities were determined and the active fractions were found in the ethyl acetate fractions of Dryopteris crassirhizoma (roots), Amomum cardamomum (roots), Triticum aestivum (seeds), Perilla sikokiana (leaves), Anemarrhena asphodeloides (roots). Especially, the ethyl acetate fraction of Dryopteris crassirhizoma (roots), which exhibited the strong inhibition against BSV COX $(IC_{50},\;65.4\;{\mu}g/ml)$, COX-I $(IC_{50},\;8.5\;{\mu}g/ml)$, and COX-II $(IC_{50},\;17.2\;{\mu}g/ml)$, is under investigation to isolate active principles using activity-guided fractionation method.

• Natural Product Sciences
• /
• v.27 no.2
• /
• pp.128-133
• /
• 2021

The Coumarin (1,2-benzopyrone) is the main secondary metabolite of Mikania laevigata Sch. Beep ex Baker and Mikania glomerata Spreng., which are popularly known as guaco. These plants have been used mainly in traditional medicine in the treatment of respiratory diseases because their bronchodilator effect. However, there are around 200 species of Mikania, which are quite similar in appearance. From these, only M. leavigata and M. glomerata have high concentrations of coumarins. In this line, the falsification of products Mikania based has been frequent. In this sense, this work demonstrated the application of the easy, fast, e not destructive method based in Nuclear Magnetic Resonance in quantitative mode (qNMR) for the determination of coumarin in both commercial and homemade guaco products. Thus, in the first step the compounds were extract from guaco leaves and syrups using chloroform (CHCl₃), with or without ultrasound. About the method, was linear with a R² = 0.9947 for 1,2-benzopyrone, with detection and quantification limits with were 0.11 and 0.36 mg mL^-1 respectively. In the same line, the method was safe with RSD <0.3% and with recovery ranging from 93-101%. To confirm the applicability of the method, in the last step was applied to 10 real samples (6 from leaves and 4 from syrups). The content of the coumarin in the leaf extract ranged from 0.62 to 1.30 mg mL^-1. For syrups I, II and IV, the content of coumarin was in accordance with the manufacturers. However, for de Syrup III, the concentration was 155% higher. In summary, the qNMR is a rapid method with minimal sample preparation that can be used to quantify coumarin in home-made plant extracts as well as in commercial samples as syrup for instance. This method is applicable for quality control of different plants-based products.

• Archives of Pharmacal Research
• /
• v.10 no.1
• /
• pp.67-68
• /
• 1987

1-eicosanoyl cafferate, mp 109-$110^{\circ}$ was isolated from the underground parts of Echinosophora koreenis together with hexacosanol, mp 75-$110^{\circ}$ 1-Eicosanoyl cafferate was isolated for the first time from plant source.

• Archives of Pharmacal Research
• /
• v.2 no.2
• /
• pp.115-119
• /
• 1979

Tabulated results are presented of a test for an activity affecting on drug metabolism of 75 plant samples frequently used in the traditional medicine in Korea.

• Proceedings of the Korean Journal of Food and Nutrition Conference
• /
• 2004.12a
• /
• pp.55-65
• /
• 2004

• Archives of Pharmacal Research
• /
• v.6 no.1
• /
• pp.25-28
• /
• 1983

From the stem bark of Albizzia julibrissin (Leguminosae) two known sapogenins, acacigenin B, and machaerinic acid lactone, were isolated and identified by chemical and spectral data. It is the second time that the former was isolate dform the plant sources.

• Archives of Pharmacal Research
• /
• v.11 no.3
• /
• pp.197-202
• /
• 1988

A new saponin, 3-O-[${\alpha}$-L-rhamnopyranosyl$(1{\rightarrow}2)$-${\beta}$-D-glucopyranosyl$(1{\rightarrow}2)$-${\beta}$-D-glucuronopyranosyl]soyasapogenol B carboxylate (6) has been isolated from the medicinal plant yellow sweetclover together with azukisaponin II(7), robinin(8), and clovin(9). 7,8, and 9 are reported for the first time from this plant. The new saponin(6) exhibited inhibitory action on leucocyte migration in inflammation.

• YAKHAK HOEJI
• /
• v.14 no.3_4
• /
• pp.51-53
• /
• 1970

Alcohol extracts of six plant namely Coix agrestis L$_{OUREIRO}$, Chloranthus Japonicus (Siebi) N$_{AKAI}$, Ajuga Spectabilis N$_{AKAI}$, Juglans mandsuria Max., Cirsium Macki var Ussuriense $K_{ITA}$ and Sasamorpha Purpurascense (Hack) N$_{AKAI}$ were subjected to the screening tests for anti-tumor activities against SN-36 Leukemia, Sarcoma 180 and Ehrlich ascites carcinoma. Of the six extracts, Ajuga Spectabilis N$_{AKAI}$ and Coix agrestis L$_{OUREIRO}$ were found to be potential in survival effect of tumor transplanted mice.

• Korean Journal of Pharmacognosy
• /
• v.3 no.4
• /
• pp.205-209
• /
• 1972

Anti-inflammatory activity of crude drugs was evaluated by the albumin stabilizing activity test, according to the screening method of Mizushima et al., upon the randomly selected samples of 63 genus, 106 families, 123 species of plant. Almost every plant belonging to the families Araliaceae, Umbelliferae and Liliaceae showed strong stabilizing activity on the heat denaturation of bovine serum albumin, suggesting the presence of anti-inflammatory components in the plants.