• Title/Summary/Keyword: Natural media

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A Study on the Dyeability of Natural Dyes of Sustainable Seaweed-Containing Fiber (지속 가능한 해초 함유 섬유의 천연염료 염색성에 관한 연구)

  • Kim, Sojin;Choi, Kyoungmi
    • Journal of Fashion Business
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    • v.26 no.3
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    • pp.87-97
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    • 2022
  • This study investigated the dyeability and color change of the natural dyes of SeaCell, a biodegradable functional fiber that is permanently added to cellulose fibers with natural additives extracted from seaweeds. The natural dyes used in the study are five dyes. Gardenia and turmeric, which are yellow-based natural dyes, Sappan wood and Lac, which are red-based natural dyes, and Indigo, a blue-based natural dye, were selected. The dyeability and color change according to the change of the mordant conditions and the number of times of dyeing were investigated. In addition, the dyeing properties and colors of cotton and silk fibers were compared under the same dyeing conditions as SeaCell. The study results are as follows. It was found that SeaCell had lower dyeing properties than silk, a protein fiber, in gardenia, sappan wood, and lac dyes, but had higher dyeing properties than cotton with the same cellulose component as SeaCell fibers. In the case of turmeric, it showed higher dyeing properties than cotton except for the no mordant condition. In the case of Indigo dye, SeaCell shows the best dyeability, indicating that it is a very suitable fiber for Indigo dyeing. As sustainable functional fibers are continuously developed in the future, natural dyes that are environmentally friendly and human-friendly are actively introduced and commercialized, and it is expected that they can be used as reference materials.

A New Synthetic Medium for Lactic Lactococci: Application to Marine lactic Acid Bacteria

  • KIM Joong K.;BAJPAI Rakesh K.
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.6
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    • pp.812-813
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    • 1995
  • Lactococcal cells are nutritionally fastidious and thus, generally cultured either in milk or M17 medium (Terzaghi and Sandine, 1975). In this study, Lactococcus cremoris wild-type (KH) and its less­proteolytic mutant (KHA1) cells were grown on the M17 medium or with modified M17 medium by replicated parallel experiments. The modified M17 medium had the same composition as M17 medium, except that lactose was replaced by glucose. Analyses of culture-broth samples, in which the M17 and the modified M17 media were used, were conducted by high-performance liquid chromatography (HPLC). But, working with these media created noisy problems in analyses of samples. Therefore, a new semi-synthetic medium was developed on the basis of nutritional requirements (Morishita et al., 1981). The composition of the semi-synthetic medium determined on the basis of the nutritional requirements and the composition of milk, is presented in Table 1. The composition of M17 medium is also presented and compared in the table. L. cremoris KH and KHA1 cells were grown again on the new synthetic medium containing glucose or lactose. The broth samples were then drawn and analyzed by HPLC. Clearer separations of fermented products were achieved from the new medium than those with the M17 and the modified M17 media. In comparison with the M17 or the modified M17 media, growth on the new medium was good (Kim et al, 1993). Additional fermentations were also carried out at a controlled pH of 7.0, where enhanced growth of lactococcal cells was obtained. In the fermentations, samples were also analyzed for the concentrations of sugar and lactic acid. The results showed that the new synthetic medium was as good as or better than the M 17 and the modified M 17 media. This is because casein hydrolysate in the synthetic medium provided a ready supply of amino acids and peptides for L. cremoris KH and KHA1 cells. Lactic acid bacteria (LAB) including Lactococcal cells have been known to be an effective means of preserving foods, at the same time as giving particular tastes in fields of dairy products. LAB also have always occupied an important place in the technology of sea products, and marine LAB have known to be present in traditional fermented products (Ohhira et al, 1988). To apply the new synthetic medium to marine LAB, two different LAB were isolated from pickled anchovy and pollacks caviar and were grown on the new media in which various concentrations of NaCl $(3, 5, 7 and 10\%)$ added. They were also grown on the medium solution in natural seawater $(35\%o\;salinity)$ and on the solution of natural seawater itself, too. As seen in Fig. 1, Marine LAB were grown best on the synthetic medium solution in natural seawater and the higher concentrations of NaCl were added to the medium, the longer lag-phase of growth profile appeared. Marine LAB in natural seawater were not grown well. From these results, the synthetic medium seems good to cultivate cells which are essential to get salted fish aged. In this study, it showed that the new synthetic medium provided adequate nutrition for L. cremoris KH and KHA1 cells, which have been used as cheese starters (Stadhouders et al, 1988). Using this new medium, the acid production capability of starter cultures could be also measured quantitatively. Thus, this new medium was inferior to the M17 or the modified M17 medium in culturing the cheese starters and in measuring fermentation characteristics of the starter cells. Moreover, this new medium found to be good for selected and well-identified marine LAB which are used in rapid fermentations of low-salted fish.

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Extracellular Wood-degradative Enzymes from Lentinus edodes JA01 (표고 균주(菌株) JA01에서 분비되는 세포외(細胞外) 목재성분(木材成分) 분해효소(分解酵素)에 관하여)

  • Hong, Soon-Woo;Shin, Kwang-Soo;Yoon, Yeup;Lee, Won-Kyu
    • The Korean Journal of Mycology
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    • v.14 no.3
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    • pp.189-194
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    • 1986
  • The aim of this study was to investigate physiological chracteristics of Lentinus edodes in Korea. We tried to detect properties of the several wood-degradative enzymes and investigate patterns of the enzyme production. A specific carbon source was used in the enzyme induction media for each enzymes, and the crude extract was used for the enzyme solution. With these enzyme solution, we investigated optimum temperature and pH conditions of their reactions. Moreover we investigated transition patterns of the enzyme production of the several wood-degrad­ative enzmes from Complex and Saw dust media for the purpose of studying the mechanisms of the wood component degradation by this fungus. It was assumed that the order of the wood com­ponent degradation was cellulose, xylan, and then pectic substances, and that the synergistic effects of these substances also influenced the degradation of wood components.

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Natural Scene Text Binarization using Tensor Voting and Markov Random Field (텐서보팅과 마르코프 랜덤 필드를 이용한 자연 영상의 텍스트 이진화)

  • Choi, Hyun Su;Lee, Guee Sang
    • Smart Media Journal
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    • v.4 no.4
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    • pp.18-23
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    • 2015
  • In this paper, we propose a method for detecting the number of clusters. This method can improve the performance of a gaussian mixture model function in conventional markov random field method by using the tensor voting. The key point of the proposed method is that extracts the number of the center through the continuity of saliency map of the input data of the tensor voting token. At first, we separate the foreground and background region candidate in a given natural images. After that, we extract the appropriate cluster number for each separate candidate regions by applying the tensor voting. We can make accurate modeling a gaussian mixture model by using a detected number of cluster. We can return the result of natural binary text image by calculating the unary term and the pairwise term of markov random field. After the experiment, we can confirm that the proposed method returns the optimal cluster number and text binarization results are improved.

Definition and Analysis of Shadow Features for Shadow Detection in Single Natural Image (단일 자연 영상에서 그림자 검출을 위한 그림자 특징 요소들의 정의와 분석)

  • Park, Ki Hong;Lee, Yang Sun
    • Journal of Digital Contents Society
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    • v.19 no.1
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    • pp.165-171
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    • 2018
  • Shadow is a physical phenomenon observed in natural scenes and has a negative effect on various image processing systems such as intelligent video surveillance, traffic surveillance and aerial imagery analysis. Therefore, shadow detection should be considered as a preprocessing process in all areas of computer vision. In this paper, we define and analyze various feature elements for shadow detection in a single natural image that does not require a reference image. The shadow elements describe the intensity, chromaticity, illuminant-invariant, color invariance, and entropy image, which indicate the uncertainty of the information. The results show that the chromaticity and illuminant-invariant images are effective for shadow detection. In the future, we will define a fusion map of various shadow feature elements, and continue to study shadow detection that can adapt to various lighting levels, and shadow removal using chromaticity and illuminance invariant images.

Cultivation Conditions for Mass Production of an Antagonistic Bacterium Stenotrophomonas maltophilia BW-13 (길항세균 Stenotrophomonas maltophilia BW-13의 대량배양을 위한 최적 배양조건)

  • Park Jong-Young;Kim Han-Woo;Kim Hyun-Ju;Chun Ok-Ju;Jung Soon-Je;Choi Woobong;Lee Seon-Woo;Moon Byung-Ju
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.158-161
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    • 2005
  • Stenotrophomonas maltophilia BW-13 is a potent biocontrol agent to control crisphead lettuce bottom rot caused by Rhizoctonia solani. To define the optimum conditions for the mass production of the S. maltophilia BW-13, we have investigated optimum culture conditions and effects of various carbon sources on the bacterial growth. The optimum initial pH and temperature were determined as pH $6.0\~7.0 and $35^{\circ}C$, respectively. For the selection of effective carbon source for the mass production, we tested the low molecular carbon sources such as sucrose, glucose, lactose, maltose, manose and the high molecular carbon source such as dough conditioner, rice bran, corn starch, sweet potato starch. As the results, the addition of dough conditioner in a basal medium ($1.25\%\;K_{2}HPO_4,\;0.38\%\;KH_{2}PO_4,\;0.01\%\;MgSO_4{\cdot}7H_{2}O,\;0.5\%\;Yeast extract$) was able to achieve higher cell density and the antifungal activity than others. Therefore, the basal medium containing $3\%$ dough conditioner (named as dough conditioner medium) was finally selected the optimized media for the mass production of BW-13 strain.

Media Reporting of Natural Disaster: the Case of Typhoon Rusa (자연재난 보도의 특성 분석: 태풍 루사의 사례 연구)

  • Kim, Man-Jae
    • Journal of the Korean Society of Hazard Mitigation
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    • v.5 no.3 s.18
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    • pp.1-9
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    • 2005
  • The primary source of disaster information for victims as well as ordinary people is mass media. In spite of their importance, the media often inaccurately portrays reality, which has stimulated academic debates. In Korea, however, media reporting patters of disaster have been hardly addressed. Therefore, the paper analyzes how newspaper and television news have reported typhoon Rusa between August 29 and October 1 in 2002 by using KINDS(Korean Integrated News Database System). The results show that television news tend to present more soft news stories emphasizing human interest stories than newspaper articles, relying on victims as primary interviewees. It is also pointed out that the Korean media do not play a significant role in providing disaster information to public regarding how to lessen the effects of impact through preparation. Disaster mythology representing wrong beliefs about human behavior in disaster is found in Korean media reporting, too. Unlike their western counterparts, however, Korean media seem to use the dependency image of helpless victims in order to stimulate donations. Analyses of disaster reporting patterns suggest that, in make disaster warning messages associated with behavioral responses, credible and official sources should provide clear and precise warning messages to the media, and the media also need to stress individual responsibilities in protecting his or her own properties not to make victims heavily dependent on public supports, while inducing donations.

Effect of Vitamin D3 on Biosynthesis of Estrogen in Porcine Granulosa Cells via Modulation of Steroidogenic Enzymes

  • Hong, So-Hye;Lee, Jae-Eon;An, Sung-Min;Shin, Ye Young;Hwang, Dae Youn;Yang, Seung Yun;Cho, Seong-Keun;An, Beum-Soo
    • Toxicological Research
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    • v.33 no.1
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    • pp.49-54
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    • 2017
  • Vitamin D3 is a fat-soluble secosteroid responsible for enhancing intestinal absorption of calcium, iron, and other materials. Vitamin D3 deficiency, therefore, can cause health problems such as metabolic diseases, and bone disorder. Female sex hormones including estrogen and progesterone are biosynthesized mainly in the granulosa cells of ovary. In this study, we isolated granulosa cells from porcine ovary and cultured for the experiments. In order to examine the effect of vitamin D3 on the ovarian granulosa cells, the mRNA and protein levels of genes were analyzed by real-time PCR and Western blot assay. The production of estrogen from the granulosa cells was also measured by the ELISA assay. Genes associated with follicle growth were not significantly altered by vitamin D3. However, it increases expression of genes involved in the estrogen-biosynthesis. Further, estrogen concentrations in porcine granulosa cell-cultured media increased in response to vitamin D3. These results showed that vitamin D3 is a powerful regulator of sex steroid hormone production in porcine granulosa cells, suggesting that vitamin D deficiency may result in inappropriate sexual development of industrial animals and eventually economic loss.

Investigation of Heavy Metals and Residual Pesticides from Imported Oak Mushroom Culture-inoculated Sawdust Media (표고균이 접종된 수입 톱밥 배지에서 중금속과 잔류농약 조사)

  • Kim, Jun Young;Kwon, Hyuk Woo;Ko, Han Gyu;Hyun, Min woo;Lee, Chan-Jung;Kim, Seong Hwan
    • Journal of Mushroom
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    • v.15 no.4
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    • pp.202-205
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    • 2017
  • The potential risk of heavy metals and residual pesticides in imported sawdust media inoculated with oak mushroom cultures is a concern for safe mushroom production in Korea. To obtain preliminary data for a sound analysis of this risk, this study was undertaken to analyze the presence of heavy metals and residual pesticides in the oak mushroom media imported from six different regions in China, between 2013 and 2015. Lead, arsenic, chrome, mercury, and cadmium were analyzed; arsenic, chrome, and mercury were not detected. Lead was detected in the imported oak mushroom media from Jinlin, Shandong, Liaoning, and Tianjin. The detection level of lead ranged from 1.7 to 3.2 mg/kg. Cadmium was detected at a level of 0.028 to 0.037 mg/kg in the media from Shandong and Liaoning. No residual pesticides were detected in any of the samples.

Quantitative and Qualitative Studies of Commensal Bacterial Flora of Clam, Ruditapes philippinarum in Hadong Area (하동 지역에 서식하는 바지락의 미생물총 분포에 관한 정량 및 정성적 분석)

  • Kim, Myoung-Sug;Park, Jun-Hyu;Ha, Jai-Yi;Huh, Min-Do;Huh, Sung-Hoi;Jeong, Hyun-Do
    • Journal of fish pathology
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    • v.11 no.2
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    • pp.143-150
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    • 1998
  • Characteristics and distribution of the natural commensal flora in the surrounding environment and tissues of clam in Hadong area were studied under varying conditions of growth media and incubation temperatures. Total numbers of bacteria present in intestinal tract, gill, body fluid and surrounding mud were found to be not influenced by the used BHIA, STA and SNA media. Although the growth rate of bacteria at the condition of $15^{\circ}C$ incubation temperature was slower than that of $25^{\circ}C$ and $35^{\circ}C$, it showed the highest number of total bacteria compared with other two different conditions of incubation temperature. Interestingly, the proportion of bacteria able to form colony on several selective media was higher in replica analysis from nutrient media to selective media than that in direct smearing from samples. The generic diversity of bacteria isolated from the tissues and analyzed by API 20E and API 20NE kit showed similar pattern with each other and distinct from that of environment. The distribution of bacteria in the surrounding mud or mantle fluid of clam indicated a high diversity comparable to that found for the gill or intestinal tract microflora, with Pseudomonas being the prevalent group. It implies that the tissues of clam may probide a selective habitat for a commensal microflora.

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