• 한국가금학회지
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• 제49권2호
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• pp.125-137
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• 2022

This study aimed to determine the effects of dietary microalgae (Tetradesmus sp. (TO)) on intestinal immunity and microbiota of pre-starter broilers. One hundred and twenty 1-day-old birds (Ross 308) were allocated to two dietary treatment groups with six blocks in a randomized complete block design. The two experimental diets consisted of a corn-soybean meal-based basal diet and a diet with 0.5% TO powder instead of cornstarch in the basal diet. After feeding the experimental diets for ten days, all birds' body weight and feed intake were measured, and representative eight birds were selected from each treatment group. Small intestinal lamina propria cells were isolated using flow cytometry to examine the frequency of immune cells. Cecal feces were harvested for 16s rRNA gut microbiota analysis and fecal IgA levels. Here, we found that 0.5% TO supplementation increased CD3⁺CD4⁺ T cells in the small intestine, but decreased CD3⁺CD8⁺ T cells in the small intestine. Gut microbial analysis showed that TO supplementation significantly increased the alpha diversity of the gut microbiome. Taxonomic analysis showed that TO treatment increased the abundance of Firmicutes and decreased that of Bacteroidetes at the phylum level. The distribution of Enterobacteriaceae containing many harmful bacteria at the family level, was lower in the TO group. In the LEfSe analysis, the TO group had a significantly enriched abundance of Agathobaculum at the genus level. Overall, results show that Tetradesmus sp. supplementation influences intestinal T-cell immunity and induces the expansion of beneficial gut microbes in pre-starter broiler chickens.

• 한국식품영양과학회지
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• 제32권8호
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• pp.1394-1400
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• 2003

본 연구에서는 비만 및 대사성증후군 위험 인자를 개선하기 위하여 한방원리를 활용한 조성물 KSH28을 구성하고 동물실험과 인체실험을 통한 효능 평가를 수행하였다. 동물실험에서는 4주령의 수컷 C57BL/6 마우스에게 30%고지방 식이를 급여하면서 4주간 KSH28을 경구 투여하였다. 4주후 체중과 복부의 부고환 및 신장후 지방조직은 KSH28에 의해 용량 의존적으로 의미있게 감소하였다. 또한 지방조직내의 지방세포 평균단면적이 KSH28에 의해 용량 의존적으로 감소하여 지방조직 억제 효과가 뚜렷함이 발견되었다. 공복시 혈당과 혈중 중성지방 농도는 KSH28에 의해 유의적으로 감소하였다. 인체실험에서는 40명 (남성 8명, 여성 32명 )의 피험자들이 KSH28을 포함한 생식 제품(30 g)을 하루 2포 4주간 복용하였다. 체중은 4주간 평균적으로 1.91 kg(남 2.05 kg, 여 1.87 kg)감소하여 주당 약 0.5 kg의 감소를 나타내었고 체성분 분석에서는 체지방이 1.77kg(남 2.69kg,여 1.56kg) 감소하여 체중감량 부분의 대부분을 차지하였다. 수분과 단백질, 근육, 무기질은 변화가 없었다. 여성에서 수축기 혈압, 확장기 혈압과 혈중 총콜레스테롤, LDL콜레스테롤이 모두 의미있게 감소하여 대사성증후군을 개선시킴이 관찰되었다. 간기능의 지표로 이용되는 GPT 수치에는 변화가 없었고 기타의 특별한 부작용은 발견되지 아니하였다.

• 생약학회지
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• 제35권2호통권137호
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• pp.143-146
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• 2004

The effects of the MeOH and water extracts from the marine algae Pelvetia siliquosa were evaluated on hyperlipidemic rats induced by cholesterol rich diet or poloxamer-407. The MeOH and water extracts, when administered orally for 3 consecutive days in hyperlipidemic rats induced by poloxamer-407 (1 ml of 30%), were found to cause a significant decrease in plasma cholesterol and triglyceride concentrations. Both extracts also showed a significant inhibition of serum total cholesterol and triglyceride level in rats treated with cholesterol rich diet. HDL-cholesterol, however, was increased significantly.

• 한국잠사곤충학회지
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• 제19권1호
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• pp.5-7
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• 1977

누에인공사료의 조성중 필수적인 cellulose성분을 천연의 자원에서 개발하기 위해 뽕나무가지, 미류나무가지, 리기다소나무와 이태리포프라의 펄프분말, 왕겨, 톱밥, 볏짚등을 대상으로 실험하였다. 실험결과 뽕나무가지와 리기다소나무펄프가 인공사료의 cellulose대치 가능 자원임이 밝혀졌다. 특히 뽕나무가지의 화학적인 처리방법에 의하여 누에성장발육에 보다 유용한 제품을 얻을 수 있었고, 나아가서 장차 인공사료개발에 cellulose원으로서의 역활을 기대할 수 있게 되었다.

• 한국식품위생안전성학회지
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• 제27권3호
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• pp.306-311
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• 2012

Stevioside, a natural sweeteners presently used in various kinds of food and food products in Korea, was evaluated for its toxicity potential in the 14 day feeding study using B6C3F1 mice. Stevioside was added to the diet at different concentrations of 0.31, 0.62, 1.25, 2.5 and 5%, and was administered for 14 consecutive days. An increase of liver organ weight in male mice was observed. No diet-related differences were noted in clinical signs, food consumption, and gross and histopatholgical evaluation. Based on these results, we concluded that the concentration of 5% in the diet was a suitable maximum tolerable dose of stevioside for a 90 day study in mice.

• Natural Product Sciences
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• 제22권4호
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• pp.231-237
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• 2016

Prolonged alcohol consumption causes alcoholic liver damage due to the generation of reactive oxygen species, the accumulation of fatty acids, and an increase in inflammatory cytokines in the liver. In this study, the protective effect of a fruit extract of Paeonia anomala (FEPA) against chronic alcohol-induced liver damage was evaluated in Sprague-Dawley rats fed an ethanol or a control Lieber-DeCarli diet for 5 weeks to induce alcoholic liver damage. FEPA (50, 25, and 10 mg/kg body weight/day) as well as the reference control silymarin (25 mg/kg body weight/day) were administered along with the ethanol diet. FEPA protected against increases in alanine aminotransferase and aspartate aminotransferase in serum and attenuated alcohol-induced increases in triglycerides, tumor necrosis factor alpha, thiobarbituric acid-reactive substances, and cytochrome P450 2E1 enzyme activity in the liver compared with the group treated with ethanol only. Anti-oxidative defenses such as the total glutathione level and glutathione peroxidase activity were increased by FEPA treatment. These results suggest that FEPA exerts protective effects against chronic alcohol-induced liver damage by attenuating hepatosteatosis and pro-inflammatory cytokine production and enhancing anti-oxidative defense mechanisms in the liver.

• International Journal of Industrial Entomology and Biomaterials
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• 제26권1호
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• pp.61-66
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• 2013

Baculum elongatum has several peculiarities such as parthenogenesis, unique external features, changing body color, and dropping oviposition, which makes it a potential economically useful insect. Oviposition was performed by females without fertilization by the sperm of males. The oviposition period was $42.2{\pm}22.7$ days and the number of eggs per female was $109.5{\pm}70.5$ eggs. The hatch rate was 73.3% at $25^{\circ}C$ and 66.7% after low temperature treatment ($8^{\circ}C$ for 60 days). In nature, B. elongatum overwinters as an egg, but it can be assumed from the results that cold temperatures were not required for hatching. The hatch rate was 98.2% in the treatment using floral foam, fermented sawdust, and leaves. The developmental period was $100.9{\pm}4.2$ days for eggs, $55.3{\pm}4.6$ days for nymphs, and $49.7{\pm}16.0$ days for adults. The length of the eggs was $0.33{\pm}0.0$ cm and the lengths of the nymphs were clearly distinguishable according to the instar stage. Clover(Trifolium repens) was an excellent diet as it was similar to the host plant and could be used as an alternative diet. The rate of reaching adulthood for the insects was 66.7% on an artificial diet containing 25% acacia leaves. For sustainable mass rearing of nymphs or adults of B. elongatum, a natural diet could be used such as acacia (Robinia pseudoacacia), white oak (Quercus aliena), chestnut (Castanea crenata var. dulcis), and bush clover (Lespedeza bicolor), or an alternative diet such as clover or artificial diet.

• Journal of Nutrition and Health
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• 제56권6호
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• pp.589-601
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• 2023

Purpose: Baicalein, a natural flavone found in herbs, exhibits diverse biological activities. Nonalcoholic steatohepatitis (NASH) is an irreversible condition often associated with a poor prognosis. This study aimed to evaluate the effects of baicalein on the development of NASH in mice. Methods: Male C57BL/6J mice were randomly divided into four groups. Three groups were fed a methionine-choline-deficient (MCD) diet to induce NASH and were simultaneously treated with baicalein (at doses of 50 and 100 mg/kg) or vehicle only (sodium carboxymethylcellulose) through oral gavage for 4 weeks. The control group was fed a methionine-choline-sufficient (MCS) diet without the administration of baicalein. Results: The baicalein treatment significantly reduced serum levels of alanine aminotransferase and aspartate aminotransferase, suggestive of reduced liver damage. Histological analysis revealed a marked decrease in nonalcoholic fatty liver activity scores induced by the MCD diet in the mice. Similarly, baicalein treatment at both doses significantly attenuated the degree of hepatic fibrosis, as examined by Sirius red staining, and hepatocellular death, as examined by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Baicalein treatment attenuated MCD-diet-induced lipid peroxidation, as evidenced by lower levels of hepatic malondialdehyde and 4-hydroxynonenal, demonstrating a reduction in oxidative stress resulting from lipid peroxidation. Moreover, baicalein treatment suppressed hepatic protein levels of 12-lipoxygenase (12-Lox) induced by the MCD diet. In contrast, baicalein enhanced the activities of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. Additionally, baicalein treatment significantly reduced hepatic non-heme iron concentrations and hepatic ferritin protein levels in mice fed an MCD diet. Conclusion: To summarize, baicalein treatment suppresses hepatic lipid peroxidation, 12-Lox expression, and iron accumulation, all of which are associated with the attenuation of NASH progression.

• 한국가금학회지
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• 제33권3호
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• pp.189-194
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• 2006

본 연구의 목적은 천연 미네랄 제제의 첨가가 산란계의 생산성, 난질 및 혈액과 난황 내 미네랄 함량에 미치는 영향을 알아보고자 실시하였다. 72주령 Hy-line Brown 240수를 공시하였고, 6주간 사양 시험을 실시하였다. 처리구는 1) Control(CON), 2) Control + 3% 키토산 + 0.50% 천연미네랄 제제 (M 0.5), 3) Control + 3% 키토산 + 0.10% 천연미네랄 제제 (M 1.0), 4) Control +3% 키토산 + 0.15% 천연미네랄 제제(m1.50)로 총 4 처리를 하여 처리당 10반복, 반복당 6수씩 임의 배치하였다. 산란율은 M1.5 처리구가 대조구, M1.0 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 하지만 난중은 M0.5 처리구가 다른 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난각 강도는 처리구와 대조구간에 차이를 보이지 않았으나, 난각 두께는 M1.5 처리구가 대조구 및 다른 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난황색은 미네랄 처리구에서 첨가 수준의 증가에 따라 감소하는 경향을 보였다. Haugh unit은 대조구와 M1.0 처리구가 가장 높게 나타났다(P<0.05). 난황 내 K의 함량은 M1.5 처리구가 대조구와 비교하여 증가하였다(P<0.05). 그러나 난황 내 Ca과 Fe 함량에 있어서 처리구간의 유의적인 차이는 없었다(P<0.05). 혈액 내 Ca과 Fe 함량은 M1.5 처리구에서 대조구와 비교하여 높게 나타났다(P<0.05). 따라서 산란계에서 1.5% 천연 복합 미네랄 제제의 급여는 산란율, 난각 두께를 개선시켰고, 혈액 및 난황 내 미네랄 조성을 변화시켰다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.195.1-195
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• 2001

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