• Biomolecules & Therapeutics
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• v.23 no.6
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• pp.597-603
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• 2015

Synthetic cannabinoids JWH-018 and JWH-250 in 'herbal incense' also called 'spice' were first introduced in many countries. Numerous synthetic cannabinoids with similar chemical structures emerged simultaneously and suddenly. Currently there are not sufficient data on their adverse effects including neurotoxicity. There are only anecdotal reports that suggest their toxicity. In the present study, we evaluated the neurotoxicity of two synthetic cannabinoids (JWH-081 and JWH-210) through observation of various behavioral changes and analysis of histopathological changes using experimental mice with various doses (0.1, 1, 5 mg/kg). In functional observation battery (FOB) test, animals treated with 5 mg/kg of JWH-081 or JWH-210 showed traction and tremor. Their locomotor activities and rotarod retention time were significantly (p<0.05) decreased. However, no significant change was observed in learning or memory function. In histopathological analysis, neural cells of the animals treated with the high dose (5 mg/kg) of JWH-081 or JWH-210 showed distorted nuclei and nucleus membranes in the core shell of nucleus accumbens, suggesting neurotoxicity. Our results suggest that JWH-081 and JWH-210 may be neurotoxic substances through changing neuronal cell damages, especially in the core shell part of nucleus accumbens. To confirm our findings, further studies are needed in the future.

• Biomolecules & Therapeutics
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• v.25 no.3
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• pp.288-295
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• 2017

The incidence of polypharmacy-which can result in drug-drug interactions-has increased in recent years. Drug-metabolizing enzymes and drug transporters are important polypharmacy modulators. In this study, the effects of bosentan and rifampin on the expression and activities of organic anion-transporting peptide (OATP) and cytochrome P450 (CYP450) 2C9 and CYP3A4 were investigated in vitro. HEK293 cells and primary human hepatocytes overexpressing the target genes were treated with bosentan and various concentrations of rifampin, which decreased the uptake activities of OATP transporters in a dose-dependent manner. In primary human hepatocytes, CYP2C9 and CYP3A4 gene expression and activities decreased upon treatment with $20{\mu}M$ $bosentan+200{\mu}M$ rifampin. Rifampin also reduced gene expression of OATP1B1, OATP1B3, and OATP2B1 transporter, and inhibited bosentan influx in human hepatocytes at increasing concentrations. These results confirm rifampin- and bosentan-induced interactions between OATP transporters and CYP450.

• International Journal of Safety
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• v.10 no.1
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• pp.10-15
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• 2011

In Japan, protective measures to reduce falls from scaffolds have been strictly applied within industry safety guidelines, and such measures have significantly decreased fatal accidents due to falls from scaffolds. However, the rate of fatal accidents from falls is still high in the construction industries. In order to examine further countermeasures to reduce such falls, the Japan Ministry of Health, Labour and Welfare established a committee in our institute. That committee$\acute{i}$s work experimentally confirmed the effectiveness of using plastic sheets as a covering around scaffolds to protect against falls of construction materials (a method widely used in Japan). However, the workers fell from the space between the work platform and the plastic sheets very occasionally. Based on those results, the plastic sheets were improved for fall protection from the space, and the effect of the improved sheets was confirmed experimentally.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.478-481
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• 2013

This study was performed to evaluate the acute skin and eye irritation of plant extracts (neem and sophora) against rabbit. The result of skin irritation test indicated that neem extracts and sophora extracts were not irritant. For eye irritation test, the result showed no irritation for neem extracts. Sophora extracts have a severe eye irritation and the symptoms have been reduced to day 3. Thus, safety guidelines for agricultural workers is considered to be needed when sophora extracts is used as an organic agricultural materials.

• Korean Journal of Food Science and Technology
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• v.46 no.1
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• pp.7-12
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• 2014

A sensitive and simple analytical method to identify flutianil residues in agricultural commodities was developed and validated using gas chromatography-electron capture detection (GC-ECD) and mass spectrometry (GC-MS). The flutianil residues were extracted with acetonitrile, partitioned with dichloromethane, and then purified using a silica solid-phase extraction (SPE) cartridge. The method was validated using pepper, sweet pepper, mandarin, hulled rice, soybean, and potato spiked with 0.02 or 0.2 mg/kg flutianil. The average recovery of flutianil was 76.5-108.0% with a relative standard deviation of less than 10%. The limit of detection and limit of quantification were 0.004 and 0.02 mg/kg, respectively. The result of recoveries and relative standard deviation were in line with Codex Alimentarius Commission Guidelines (CAC/GL 40). These results show that the method developed in this study is appropriate for flutianil identification and can be used to maintain the safety of agricultural products containing flutianil residues.

• Safety and Health at Work
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• v.15 no.3
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• pp.292-299
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• 2024

Background: The aim of this research is to analyze the transformation of workers perceptions of the safety climate in an oil and gas company in Indonesia when they experience a change in ownership from a multinational to a national company. Methods: This cross-sectional study used questionnaires distributed offline and online in three periods of ownership of Company X. Data analysis was carried out descriptively by comparing workers perception scores regarding the safety climate at Company X when managed by the multinational holder, transition period, and national holder. Results: Workers perceptions of the safety climate in Company X when it experienced a change in ownership from a multinational company to a national company has a trend of decreasing scores (from 8.07 to 7.48). Overall, a decreasing trend in scores occurred in several sub-variables of safety climate, namely management commitment (8.33 to 7.56), communication (8.10 to 7.64), safety priority (8.55 to 7.68), personal appreciation of risk (8.25 to 5.48), involvement (7.50 to 7.36), and personal priority and need for safety (8.25 to 5.48). Conclusions: Ownership changes cause a trend of decrease in employee perceptions of the safety climate at Company X. Company's priority on safety related to production target factors is decreasing due to the change of ownership. On the other hand, the national period had a higher score in supportive environments and work environments, compared to the multinational period.

• Journal of Food Hygiene and Safety
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• v.25 no.3
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• pp.278-280
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• 2010

This study was aimed to develop a novel qualitative multiplex polymerase chain reaction (PCR) for simultaneous detection of genetically modified (GM) soy and maize within a single reaction. The specific primers designed to detect four respective GM events (A2704-12, MON88017, Bt11, and MON863) were included in the tetraplex PCR system. Each of PCR products for four GM events could be distinguished by agarose gel based on their different lengths. The specificity and reproducibility of this multiplex PCR were evaluated. This multiplex PCR consistently amplified only a fragment corresponding to a specific inserted gene in each of the four GM events and also amplified all four of the PCR products in the simulated GM mixture. These results indicate that this multiplex PCR method could be an effective qualitative detection method for screening GM soy and maize in a single reaction.

• Journal of the Korean Society of Safety
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• v.17 no.2
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• pp.69-75
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• 2002

Current fire safety in the construction sites has developed piecemeal over a considerable period of time. However, the law and regulation of fire safety is insufficient to protect fro in the construction sites. The frequency of fire in the construction sites is very rare but it brings longterm deterioration durability of building structure and large scale industrial calamity. There are many process of works and each work deals with many kind of flammable material, so it is very difficult to find a proper safety fire prevention. This study executed the questionnaire and fire experiment of the welding work to provide effective fire prevention counterplan.

• Journal of Construction Engineering and Project Management
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• v.2 no.3
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• pp.48-57
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• 2012

With increasing number of serious construction disasters such as death, a safety management in real-time is more required recently. One of the ways to support the needs, a convergence with Information Technology (IT) has been noticed. Although number of inefficiencies including a limitation of IT equipment (weight, control inconveniences) has been concerned, data exchanging and processing fields have been improved at a wide range with an advance of mobile communication and an introduction of smart mobiles that are simple and convenient. In this research, a framework is realized in connection with smart mobile to find the problems of current safety management and derive demands in order to support effective safety management. Two smart mobile applications, Safety Management application and Safety Guideline application, enable a real-time safety management and less re-work by provided safety information.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.3
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• pp.480-484
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• 2015

As the increasing national power crises due to various environmental factor, power market change is needed more developed and systemized DSM(Demand Side Management) search at DR market. To overcome national power crisis outbreaking every year and manage the eletric power reserve ratio, we need to construct and develop the electric safety manager and general public main oriented national power plant. For the advanced national power demand management system, it is required the application development like OpenAPI, Big Data Acquisition, Web/APP basd on ICT.