• 동의생리병리학회지
• /
• 제20권5호
• /
• pp.1315-1320
• /
• 2006

The root and rhizomes of Nardostachys chinensis belonging to the family Valerianaceae has been used for medicinal therapy in Korean traditional medicine. The parts have been especially used to elicit stomachic and sedative effects. Our previous studies reported that the water extract of N. chinensis has induced granulocytic differentiation inhuman promyelocytic leukemia (HL-60) cells. The Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases involved in the regulation of various cellular responses, such as cell proliferation, differentiation and apoptosis. In this study, we investigated the signaling pathways on the HL-60 cell differentiation induced by N. chinensis. Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. chinersis. Activation of p38 increased slightly at 24 h after N. chinensis treatment, but activation of c-jun N-terminal kinase (JNK) was unaffected. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. chinensis in HL-60 cells. In contrast, p38 inhibitor (SB203580) did not inhibit the cell differentiation. These results indicated that activaiton of ERK may De involved in HL-60 cell differentiation induced by N. chinensis.

• 동의생리병리학회지
• /
• 제25권2호
• /
• pp.189-194
• /
• 2011

Nardostachys chinensis (N. Chinensis) belonging to the family Valerianaceae have been used in traditional medicine to elicit stomachic and sedative effects. The present study investigated the effects of water extract of N. Chinensis in human lymphoma U937 cells. The proliferation of U937 cells was decreased by N. Chinensis. Anti-proliferative effect of N. Chinensis on U937 cells was associated with G0/G1 phase arrest, which was mediated by regulating the expression of p21 and p27 protein. In addition, the levels of CDK2, CDK4, CDK6, Cyclin D3, and Cyclin A were decreased, but Cyclin D1, Cyclin D2 and Cyclin E were essentially undetectable. N. Chinensis induced the differentiation of U937 as shown by increased expression of differentiation surface antigen CD11b, but not CD14. Taken together, these results demonstrated that N. Chinensis potently inhibits the proliferation of U937 cells via the G0/G1 phase cell cycle arrest in association with p21 and p27, and induces granulocytic differentiation.

• 식물분류학회지
• /
• 제38권2호
• /
• pp.163-177
• /
• 2008

마타리족(Patrinieae $H{\ddot{o}}ck$, Valerianaceae)에 속하는 마타리속과 Nardostachys속의 17분류군(14종과 추가된 2아종, 1변종)의 화분을 광학현미경과 주사전자현미경, 그리고 투과전자현미경으로 관찰하였다. 화분의 크기는 주로 중립($37.41{\times}43.60{\mu}m$ - $45.65{\times}48.50{\mu}m$)에서 대립($54.88{\times}59.41{\mu}m$ - $61.70{\times}71.00{\mu}m$)까지 나타나고, 발아구는 3구형이며 드물게 4구형이다. 화분립 발아구의 가장자리에는 'halo'라는 마타리과 화분에서 나타나는 공통적인 형질을 갖고 있으며, 화분립의 모양은 적도면에서 단구형에서 약장구형이고, 극축면은 3갈래로 갈라지거나 원형이다. 마타리족의 화분은 표면무늬에 따라서 크게 두가지 유형으로 구별되어진다; (1) Type I: 과립상돌기(verrucae)가 약간 발달하거나 거의 없고, 자상돌기(echinae)가 과립상돌기 위에 발달하며 미세자상돌기(microehinae)가 산재한 형태(Nardostachys속, 마타리속의 Paleopatrinia절, Monanadropatrinia절), (2) Type II: 과립상 돌기가 잘 발달하고, 자상돌기가 과립상돌기 위에 매우 크게 발달하며 미세자상돌기가 조밀하게 화분표면을 덮고 있는 형태(마타리속의 Centrotrinia절). 투과전자현미경을 이용한 연구에서 원주층은 기저층으로부터 과립상돌기로 연결되고, 표벽의 두께는 극과 적도에서 동일하다. 본 연구결과를 통해 마타리족의 분류체계가 검토되었다.

• 대한한약학회:학술대회논문집
• /
• 대한한약학회 2007년도 Autumn Conference
• /
• pp.178-178
• /
• 2007

• 한국미생물·생명공학회지
• /
• 제51권1호
• /
• pp.43-52
• /
• 2023

The purpose of this study is to confirm the antioxidant, black hair, and hair growth effects of the N. jatamansi, O. basilicum, and C. sativus mixed extracts that pharmacological efficacy has been verified. Accordingly, four samples (NOC-1 to-4) produced under different extraction conditions were prepared and the results are as follows. First, all samples showed an increase in antioxidant content in a concentration-dependent manner from the results of antioxidant efficacy. In particular, NOC-4 extracted by steaming and ultrasonic methods showed the highest antioxidant effect among the four samples. As a result of analysis of the amount of melanin production in mouse melanoma cells, NOC-4 with concentration of 500 ㎍/ml showed higher melanin production compared to the control group, so the black hair efficacy was the best. Also, in the hair growth test results, it was found that the hair growth was the best at 0.94 ± 0.10 mm at experimental group orally administered with 500 mg/kg of NOC-4. In addition, as a result of cytotoxicity analysis in mouse melanoma cells, the safety of samples was demonstrated by maintaining cell viability of 95% or more at all concentrations. These results suggest that the steaming and ultrasonic extraction method increased the extraction yield of active ingredients for antioxidant, melanin, and hair generation, thereby affecting physiological activity. Based on these results, if the steaming and ultrasonic extraction methods are applied to the mixed extraction of N. jatamansi, O. basilicum, and C. sativus, it is judged that the practical potential as a natural material for black hair and hair growth agents will increase.

• 한국자원식물학회지
• /
• 제6권1호
• /
• pp.45-51
• /
• 1993

Many plants collected at Japan, China, Korea, Imdonesia and South America were applied to antitumor and / or cytotoxic screening tests against Sarcoma 180 ascites in mice and / or V-79, KB, P388 cultured cells. On the course of these screening tests, alcoholic extracts of Forsythia viridissima (Oleaceae), Eurycoma longifolia(Simaroubaceae), Rubia cordifolia and R. akane(Rubiaceae), Cissampelos pareira and Abuta concolor (Menispermaceae), Nardostachys chinensis (Valerianacese), Mansoa alliaceae (Bignoniaceae), Casearia sylvestris (Flacourtiacear), Maytenus ilicifolia (Celastraceae), Hedychium coronarium (Zingiberaceae), Croton palanostigma(Euphorbiaceae), Cocculus trilobus(Menispermaceae), Ginkgo biloba(Ginkgoaceae), Alpinia galanga and Cucculus zanthorrhiza(Zingiberaceae), Evodia rutaecarpa(Rutaceae), and Periploca sepium(Asclepiadaceae) showed significant activity and their active principles were clarified. In this paper, a few antitumor substances in above plants are introduced.

• 약학회지
• /
• 제45권5호
• /
• pp.522-528
• /
• 2001

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

• 생약학회지
• /
• 제44권2호
• /
• pp.161-167
• /
• 2013

Aldose reductase (AR) has been shown to play an important role in the development of the diabetic complications. To discover novel treatments for diabetic complications from natural sources, 59 Chinese herbal medicines have been investigated for inhibitory activities on AR. Among them, 10 herbal medicines, Catalpa fargesii (stem and leaf), Saussurea Laniceps(whole plant), Alnus nepalensis(stem and leaf), Swertia macrosperma (whole plant), Woodfordia fruticosa (stem and leaf), Elsholtzia bodinieri (whole plant), Elsholtzia fruticosa (whole plant), Rosa multiflora (fruit), Nardostachys chinensis (whole plant), Eurya groffii (stem and leaf) exhibited a significant inhibitory activity compared with 3,3-tetramethyleneglutaric acid (TMG) as positive control. Particularly, 4 herbal medicines, C. fargesii (stem and leaf), S. Laniceps (whole plant), A. nepalensis (stem and leaf), S. macrosperma (whole plant) showed two times more potent inhibitory activity than TMG ($5.37{\mu}g/ml$).

• Journal of Forest and Environmental Science
• /
• 제30권3호
• /
• pp.253-258
• /
• 2014

The study attempts to assess the status, distribution, conservation and use value of Medicinal and Aromatic Plant (MAPs) in the Sagarmatha National Park. Altogether 62 species of MAPs belonging to 47 genera and 33 families have been recorded in the study area. 10 species, belonging to 9 families are categorized as the potential species. Out of the these species, most potential in local but threatened species are Allium hypsistum Stearn, Cordyceps sinensis Sacc, Dactylorhiza hatagirea Soo, Nardostachys grandiflora DC, Aconitum orochryseum, Ephedra gerardiana Wall. Ex. Stapf, Swertia multicaulis D. Don, Picrorhiza scrophulariflora Penne, Rheum australe. D. Don, Malva verticillataL and Swertia pedicallata Benerji. By analysis of data using Simpson's diversity index (SI) and Shannon weaver function (H'), there was high diversity (more heterogeneous) MAPs species composition in the Manjo Gate to Large Dobhan. (0.98349). Less diversity (less heterogeneous) MAPs species composition was in Tyanboche to Pangoche (0.90419). Similarly, the Shannon weaver function shows that in plots laying out in Mongla to Phorche are evenly distributed than others However, higher MAPs species (i.e., 31) was found in the way of Manjo Gate to Larja Dobhan than others.

• 동의생리병리학회지
• /
• 제25권1호
• /
• pp.29-36
• /
• 2011

Nardostachyts chinensis (N. chinensis) belonging to the family Valerianaceae has been used to elicit stomachic and sedative effects. The MAPKs are serine/threonine kinases involved in the regulation of various cellular responses, such as cell proliferation, differentiation and apoptosis. The PKC also plays a key role in regulating the response of hematopoietic cells to both physiological and pathological inducers of proliferation and differentiation. This study investigated the signaling pathways on the U937 cell differentiation induced by N. chinensis. N. chinensis induced the differentiation of U937 cells, as shown by increased of differentiation surface antigen CD11b. Activation of ERK increased time-dependently in differentiation of U937 cells induced by N. chinensis, but activations of JNK and p38 were unaffected. Inhibitor of ERK (PD98059) significantly reduced CD11b expression induced by N. chinensis in U937 cells. In addition, N. chinensis increased protein level of PKC ${\beta}$I and PKC ${\beta}$II isoforms, but the protein level of PKC ${\alpha}$ and PKC ${\gamma}$was constant. PKC inhibitors (GF 109203X and H-7) inhibited U937 cell differentiation and the ERK activation induced by N. chinensis. These results indicated that PKC and ERK may be involved in U937 cell differentiation induced by N. chinensis.