• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.422-429
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• 2008

In this work, we analyzed the activity of control enzymes of flower color biosynthesis, chalcone synthase (CHS) and flavanone 3-hydroxylase (FHT) using biochemical and enzymological methods in Lilium longiflorum and 11 Lilium cultivars. The results obtained are as follows ; Naringenin (NAR) was synthesized in all Lilium cultivars tested by the catalytic activity of CHS which used malonyl-CoA and 4-coumaryol-CoA as substrates. Substrate-specific activity of CHS was observed because eridictiol (ERI), which uses caffeoyl-CoA as a substrate, was not detected in tested cultivars. In next step, dihydroflavone product was synthesized by FHT using flavanones as a substrate. FHT synthesized dihydrokaempferol (DHK) by using NAR as substrates. A remarkable activity of FHT was observed in other 11 cultivars.

• Annals of Hepato-Biliary-Pancreatic Surgery
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• v.26 no.2
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• pp.125-132
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• 2022

Backgrounds/Aims: It is generally accepted that non-anatomical resection (NAR) in colorectal liver metastasis (CRLM) has comparable safety and efficacy compared to anatomical resection (AR); however, there are reports that AR may have better outcomes in KRAS mutated CRLM. This study aimed to determine the effects of KRAS mutations and surgical techniques on survival outcomes in CRLM patients. Methods: Two hundred fifty patients who underwent hepatic resection of CRLM with known KRAS mutational status between 2007 and 2018 were analyzed. A total of 94 KRAS mutated CRLM and 156 KRAS wild-type CRLM were subdivided by surgical approach and compared for short- and long-term outcomes. Results: In both KRAS wild-type and mutated type, there was no difference in estimated blood loss, postoperative complications, and 30-day mortality. There was no difference in disease-free survival (DFS) between AR and NAR in both groups (p = 0.326, p = 0.954, respectively). Finally, there was no difference in intrahepatic DFS between AR and NAR groups in both the KRAS groups (p = 0.165, p = 0.516, respectively). Conclusions: The presence of KRAS mutation may not be a significant factor when deciding the approach in simultaneous resection of CRLM.

• The Korean Journal of Ecology
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• v.18 no.2
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• pp.255-263
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• 1995

Two poplar clones, I-214 (Populus euramericana) and Peace (P. Koreana × P. trichocarpa), were grown for 21 days in growth chambers at different CO₂concentrations (350, 700 and 2,000 μL·L-1). I-214 has stomata responding to environmental conditions in normal ways and Peace has unresponsive stomata to environmental factors including light, ABA, water stress and CO₂. In both plants, elevated CO₂ stmulated the growth of plant parts, especially leaf dry weight. And a CO₂ enrichment of 700 μL·L-1 CO₂ caused increment of net assimilation rate (NAR). The growth responses of these plants to CO₂ enrichment were different especially at high CO₂ condition (2,000 μL·L-1 CO₂). The total dry weight in Peace increased up to 2,000 μL·L-1 CO₂ but not in I-214. A CO₂ enrichment of 2,000 2,000 μL·L-1 CO₂ had little effect on NAR of I-214 but enhanced NAR of Peace. Although it is uncertain whether the different responses to CO₂ enrichment between I-214 and Peace resulted from the different properties of stomatal responses to long-term CO₂ treatment, the decrease in NAR is probably due in part to CO₂-induced stomatal closure in I-214 but not in Peace.

• Journal of the Korean Dietetic Association
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• v.28 no.3
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• pp.169-181
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• 2022

This study evaluated the correlation between serum uric acid level and the nutrients adequacy ratio (NAR) using data from the Korea National Health and Nutrition Examination Survey (KNHANES) 2016~2019. This is a cross-sectional study in which 6,579 Korean male adults were divided into quartiles according to their uric acid levels. All analyses were performed with adjusted age. Uric acid decreased as the age increased from the age group 19~29 years to 30~49 years and then to 50~64 years. Interquartile results of serum uric acid levels showed that increases in the serum uric acid levels were associated with decreases in LDL-cholesterol (P<0.01), triglycerides (P<0.001), body weight (P<0.001), waist circumference (P<0.001), body mass index (P<0.001), systolic blood pressure (SBP) (P<0.001), diastolic blood pressure (DBP) (P<0.001). However, uric acid levels decreased with an increase in HDL cholesterol (P<0.001). The analysis of the NARs according to the quartiles of serum uric acid levels in men showed that an increase in serum uric acid levels was associated with a decrease in the NAR levels of dietary fiber (P<0.001), calcium (P<0.001), and folic acid (P<0.001).

• Journal of the Korean Dietetic Association
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• v.8 no.3
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• pp.240-249
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• 2002

The purpose of this study was to investigate the effects of alcohol intake on nutritional intake status among the college students. The subjects were divided three groups; no-alcohol group(n=78), alcohol group(n=113), and high-alcohol group(n=82) according to intake and frequency of alcohol. And they were observed characteristics of alcohol drinking, dietary behavior and attitude, and nutrient intakes using questionnaires. The frequency of skipping meals in breakfast in high-alcohol group were higher than in other two groups(p<0.01). There were no significant differences in frequency of skipping meals of lunch and supper, reason why skipped meals, and total score of dietary attitude among three groups. However, the score of dietary attitude about no heavy drinking and smoking in high-alcohol group was higher than in other two groups(p<0.01). There were significant difference in vitamin B2 intake, alcohol intake, and energy intakes from carbohydrate, lipid, and alcohol among three groups(p<0.05, p<0.05, p<0.01, p<0.05, p<0.05). Compared energy intake of high-alcohol group with other two groups, intake from carbohydrate was low and intakes from lipid and alcohol were high. In the nutrient density, plant protein and carbohydrate in no-alcohol group, lipid and vitamin B2 in alcohol group, and animal lipid in high-alcohol group were highest among three groups. NAR and MAR were not significant difference among three groups except that NAR of P in alcohol group is highest among three groups(p<0.05). In conclusion, students high consuming alcohol have unhealty dietary behaviors in the light of high frequency of skipping breakfast, undesirable dietary attitude of alcohol drinking and smoking, and low energy inake from diet. Therefore, this results should be considered in meal management and nutritional education for student of high alcohol consumption.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.986-992
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• 2001

To assess the food intake and diet quality of middle school students who live in Kangneung area, a dietary survey using 3-day food record was conducted with 226 subjects. Seventy five percent of total food intake was in the form of plant foods and the rest in the form of animal food. Diet qualify was assessed by food group pattern, dietary diversity score (DDS), and dietary variety score (DVS). When counting the major food groups consumed, 53% of subjects had a DDS of 3 and 35% of subjects had a DDS of 4. When investigating the consumption pattern of the major five food groups, only 10.2% of subjects consumed foods from all five groups. The groups most frequently missed were dairy products (73.0%) and fruits (71.7%). On average, subjects habitually consumed 22.6 different foods daily, with the mean score of diet variety for males (22.1) being significantly lower than for females (22.9). Correlation coefficients between nutrient adequacy ratio (NAR) and DVS ranged from r=0.40 for vitamin B$_1$to r=0.61 for phosphorus. NAR also improved as the number of foods or food groups consumed increased (p<0.001). Associations between the NAR and high level of DVS was more positive than those between the NAR with DDS. When assessing the dietary quality of subjects using DDS and DVS, many people appeared not to have a desirable food intake. Therefore, to lead them to have nutritionally balanced diet, educating students and their parents on nutrients and suggesting guidelines for a desirable diet is considered to be essential so that they can intake from all of major food groups and have various foods in their diet.

• Bulletin of the Korean Chemical Society
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• v.22 no.8
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• pp.903-908
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• 2001

The gene aeg-46.5, which is expressed under anaerobic condition, has putative triple -10 regions and four transcription start sites. The mRNA transcription level and its start point change depending on the aerobic/anaerobic growth condition. RNA polymerase and its regulatory proteins must choose which of three -10 region to use. The putative triple 10 region was mutated to make only one of them function with consensus -10 region sequence (TATAAT) and the other two as non-functional region. The results show that the second and third -10 regions are used for the aerobic/anaerobic expression. The third -10 region is responsible for the high aerobic to anaerobic switch ratio. This suggests that only the last two of the putative triple -10 region have functions on aeg-46.5 gene expression switch control. The phenotype of the mutated promoter was tested in the wild type cell and narL - cell. The results indicate that the control by NarL is independent from the selection of -10 region. The expression patterns on multi-copy plasmids and on single-copy chromosome were compared. These results show that the aerobic/anaerobic switch control of aeg-46.5 is through the choice of -10 region. The mechanism of choosing different -10 region remains to be seen.

• The Korean Journal of Ecology
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• v.25 no.5
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• pp.349-352
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• 2002

The effects of Mn on growth and nutrient status of Pinus densiflora seedlings grown in a nutrient culture solution were investigated. Mn concentrations was added as manganese chloride at 0, 30 and 60ppm to the nutrient culture solution. The 2-year-old seedlings were transplanted into the solution maintained at pH 4.0, and grown for 90 days in a greenhouse. The Mn treatment induced a significant reduction in the dry weight growth of the seedlings. The relative growth rate(RGR) and net assimilation rate(NAR) of the seedlings decreased with increasing Mn concentrations in the nutrient culture solutions. For the nutrient status of the seedlings, Ca and Mg content in trunk and root was least in 60ppm Mn treatment, and Mn content in needle was about 3 times more than in root. Also the net photosynthetic rate of the seedlings was significantly lower both in 30ppm and 60ppm Mn treatment compared to them in 0ppm. This result suggests that the reductions in the RGR and NAR of the seedlings may be resulted from the inhibition of net photosynthesis by the mixed effect of lower nutrient uptake of roots and excess accumulation of Mn in needle.

• Journal of Nutrition and Health
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• v.31 no.3
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• pp.343-353
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• 1998

To assess the food intake and diet quality of Korean adults living in rural areas , a dietary survey using a one day 24-hour recall method was conducted with 2037 subjects over 30 years of age living in Yeonchon-gun , Kyungki province of Korea. Eighty percent of total food intake was in the form of vegetable food and the rest in the form o fanimal food . Diet quality was assessed by food group pattern, dietary diversity score(DDS), and dietary variety score(DVS). When counting the major food groups consumed (DDs), 47% of subjects had a DDS of 3 and 31% of subjects had a DDs of 4. On average, subjects habitually consumed 14.9 different foods daily, with the mean score of diet variety (DVS) for males(14.4) being significantly lower than for females (15.2). Persons who had higher DDSS also had higher DVSS(p<0.001). As the DDs increased , MAR(Mean adequacy ratio) improved. Correlation coefficients between NAR(nutrient adequacy ratio) and DVS ranged from r=0.34 for vitamin C to re=0.51 for vitamin B$_2$. NAR also improved as the number of foods or food groups consumed increased (p<0.001). Associations between the NAR of most nutrients with DVS was better than those with DDS. Based on these results, the food intake of the study subjects was not adequate, especially with regards to the dairy and fruit groups . Dietary diversity (DDS) and especially dietary variety (DVS) would be useful in assess nutrient intake because of their associations with total diet quality.

• Journal of Nutrition and Health
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• v.42 no.1
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• pp.48-58
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• 2009

This study was conducted to investigate effects of nutrition education and exercise intervention on health and diet quality of middle-aged women. The subjects were 209 women who wanted to involve in the health promotion program run by Jincheon-Gun Health Center. Nutrition education was given once a week and dance sports were done twice a week for 3 months. Nutrient intakes were assessed by 24-hour recall method and food records. Dietary habits and nutrition knowledge were investigated by self-administered questionnaires before and after the intervention program. Body and blood compositions were measured before and after the program. The nutrient adequacy ratio (NAR) was significantly increased in energy, protein, vitamin A, vitamin B2 and calcium, mean adequacy ration (MAR) was significantly increased (p < 0.05), and also the index of nutritional quality (INQ) was significantly increased in protein (p < 0.01). Percent (%) RIs of protein (p < 0.01) and calcium (p < 0.05) were significantly increased and body weight (p < 0.001), body fat (p < 0.01), soft lean mass (p < 0.01) and BMI (p < 0.001) decreased. Over the 3-month study, there was a significant trend toward higher HDL-C and albumin levels and lower LDL-C level (p < 0.001). The results showed that nutrition education and exercise intervention could improve the body health and the diet quality.