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Analysis of Chalcone Synthase and Flavanone 3-Hydroxylase Activity in Lilium Cultivars  

Yu, Sun-Nam (Department of Biotechnology, Catholic University of Daegu)
Publication Information
Korean Journal of Breeding Science / v.40, no.4, 2008 , pp. 422-429 More about this Journal
Abstract
In this work, we analyzed the activity of control enzymes of flower color biosynthesis, chalcone synthase (CHS) and flavanone 3-hydroxylase (FHT) using biochemical and enzymological methods in Lilium longiflorum and 11 Lilium cultivars. The results obtained are as follows ; Naringenin (NAR) was synthesized in all Lilium cultivars tested by the catalytic activity of CHS which used malonyl-CoA and 4-coumaryol-CoA as substrates. Substrate-specific activity of CHS was observed because eridictiol (ERI), which uses caffeoyl-CoA as a substrate, was not detected in tested cultivars. In next step, dihydroflavone product was synthesized by FHT using flavanones as a substrate. FHT synthesized dihydrokaempferol (DHK) by using NAR as substrates. A remarkable activity of FHT was observed in other 11 cultivars.
Keywords
Lilium; Enzymology; Flavonoid biosynthesis; Chalcone synthase; Flavanone 3-hydroxylase;
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