• Journal of Plant Biotechnology
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• 제2권1호
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• pp.51-54
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• 2000

Leaf explants of the cucumber (Cucumis sativus L.) were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of $\alpha$-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Direct shoot orgnogenesis as well as callus formation with somatic embryos and multiple shoots was observed from leaf explants of cvs. Shinhukjinju and Chungjang. The highest frequency of shoot formation 80% was observed on MS medium supplemented with NAA/BAP (5.0 ${\mu}{\textrm}{m}$/2.5 ${\mu}{\textrm}{m}$), with explants forming 3-7 shoots. Shoots formation occured within 3 to 4 weeks. Only one subculture of calli was required for plant regeneration on normal growth regulator-free medium. Plantlets transferred to soil developed into plants of normal appearance, which flowered and set fruits.

• 한국조경학회지
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• 제19권2호
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• pp.41-51
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• 1991

In order to find out the basic factors governing the rooting ability of pine cutting, the rooting of cuttings of Pinus thunbergii were examined under various conditions. A total of 5 different solutions of growth hormones made up of 3-indole butyric acid, naphthalene acetic acide and rootone were employed at three different periods of year, the middle of June, the end of September and the early of December. Cuttings prepared in length of 4cm gave better results than those of control ones. Cuttings taken from the 1-0 seedlings were superior to those from the 7-year-old trees. Seasonal variation, i.e., time of collecting cutting materials, played great important role on rooting. The majority of roots were originated from the callus lamp formed at the base of cut surface.

• Plant Resources
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• 제8권3호
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• pp.286-292
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• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.177-186
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• 2011

This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing $60{\mu}M$ ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: $1.5mg\;L^{-1}$ 1-naphthalene acetic acid (NAA), $1.5mg\;L^{-1}$ indole-3-butyric acid (IBA) and $1mg\;L^{-1}$ IBA with $0.5mg\;L^{-1}$ NAA (MIX). The effect of the photon flux ($120mmol\;m^{-2}\;s^{-1}$ and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

• KSBB Journal
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• 제24권1호
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• pp.59-64
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• 2009

The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 ${\mu}M$ NAA (${\alpha}$-naphthalene acetic acid) and 3-7 ${\mu}M$ kinetin under incubation in the dark at $25^{\circ}C$ for 6 weeks. The hot water extract ($100^{\circ}C$, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.

• Journal of Forest and Environmental Science
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• 제29권4호
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• pp.275-281
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• 2013

To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 ${\mu}M $induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 ${\mu}M $ thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 ${\mu}M $ treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 ${\mu}M $ showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.60-60
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• 2020

To establish in vitro axillary bud culture conditions of Echinosophora koreensis Nakai, one of Korean endemic endangered species famous for beautiful flowers, we tested the influence of plant growth regulators (PGRs) in shooting and rooting stage from in vitro plants. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the media induced 2.5 to 3 shoots per bud during 4 weeks of culture. And media including 0.5 mg L^-1 thidiazuron (TDZ) produced 3 to 4 shoots per bud. However, zeatin and isopentenyl adenine (2-ip) were not successful to increase shoot number, and the combination treatments of BA with other PGRs were also not effective. Shoots were smaller than 2 cm in length, in most of the treatments. In rooting, naphthalene acetic acid (NAA) treatments in the range of 0.5 to 4.0 mg L^-1 appeared to increase rooting rate by 10% to 60% approximately when compared with the control but roots developed with callus clusters. Indole butyric acid (IBA) addition had little effect on rooting (below 10%), while some roots were longer than in NAA treatments and some shoots were longer on high IBA concentrations (4.0 to 8.0 mg L^-1). It is suggested that micropropagation is a highly applicable and promising to multiplication and conservation of rare and endangered endemic species.

• 한국식품과학회지
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• 제14권2호
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• pp.179-183
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• 1982

Stevia엽(葉) 조직(組織)에서 callus의 유기(誘起) 및 배양(培養)을 위(爲)한 조건(條件)을 조사(調査)하고 이들 배양(培養) callus가 감미성분(甘味成分)인 stevioside를 생성(生成)하는지를 조사(調査)하여 다음과 같은 결과(結果)를 얻었다. Callus유기(誘起)에는 Linsmaier-Skoog기본배지(基本培地)에 benzyl-adenine $10^{-6}M$과 ${\alpha}-naphthalene$ acetic acid $10^{-5}M$을 함유(含有)한 배지(培地)가 가장 효과적(效果的)이였으며 callus의 성장률(成長率)도 비교적 양호(良好)하였으며 이들 callus는 배양후(培養後) 15일(日)부터 20일(日) 사이에 가장 빠른 성장률(成長率)을 나타내었다. Stevia callus가 생성(生成)한 stevioside는 TLC에 의(依)해 동정(同定)하였으며, 이는 정제(精製)한 표준품(標準品)의 stevioside와 동일(同一)한 Rf값인 0.50을 나타내었다. 또한 stevioside을TLC-FID analyzer에 의(依)해 분석(分析)한 결과(結果), 배양(培養) callus 건물중(乾物重) 100g당(當) 260mg이였다.

• 원예과학기술지
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• 제19권3호
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• pp.315-319
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• 2001

본 연구는 배추(Brassica campestris L. ssp. pekinensis)의 자엽 및 하배축 조직으로부터 효율적인 식물체 재분화 체계를 확립하기 위하여 실시하였다. 다양한 조합의 식물생장조절제 NAA와 BA를 조사한 바 자엽은 NAA 2.0mg/L, BA 1.0mg/L와 $AgNO_3$ 16.7mg/L를 첨가한 배지에서 절편당 2.67개의 가장 좋은 신초발생을 보였다. 하배축 절편체로부터는 NAA 1.0mg/L, BA 5.0mg/L와 $AgNO_3$ 16.7mg/L를 첨가하였을 때 절편당 1.87개의 가장 좋은 신초발생을 보였다. 재분화된 신초는 발근배지에서 뿌리를 유기하였으며 식물체는 습도가 유지된 생장상에서 순화를 거처 온실에서 재배하였다. 재분화 개체는 표현형과 염색체수에서는 이상이 없는 것으로 나타났다.

• 한국자원식물학회지
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• 제25권2호
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• pp.277-284
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• 2012

본 연구는 기내에서 발아된 까마중 유식물체의 조직별로 신초 및 뿌리 유도를 통해 효율적인 재분화 조건을 확립하고자 실시하였다. 신초 형성은 식물체 부위별로 서로 달랐으며, 잎에서의 신초 형성빈도가 자엽이나 하백축 그리고 상배축보다 높았다. 절편체당 신초의 수는 잎과 자엽에서 다소 많았으며, 신초의 길이도 다른 부위에 비해 잎과 자엽에서 빠른 증식과 신장을 나타내었다. 특히, 2.0 mg $L^{-1}$ BAP가 첨가된 배지에서 재분화율, 신초의 수와 길이가 증가하여 2.0 mg $L^{-1}$ BAP가 고빈도 신초의 유도 및 생장에 효율적인 것으로 판단되었다. 뿌리분화는 IBA와 IAA가 NAA보다 더 효과적인 것으로 나타났으며, 0.05 mg $L^{-1}$ IAA가 첨가된 배지에서 뿌리 형성율이 90%이며, 뿌리의 갯수와 길이도 각각 평균 4.0개와 7.82 cm로 가장 양호하게 나타났다. 더욱이 배양토에 이식한 재분화체는 100%의 생존율을 보였으며 정상적인 식물체로 생장하였다. 이러한 결과를 토대로 2.0 mg $L^{-1}$ BAP 조건에서의 잎 절편에서 다발성 신초 유도, 그리고 0.05 mg $L^{-1}$ IAA 조건에서의 뿌리형성 유도가 까마중의 대량번식을 위한 최적의 조건임을 알 수 있었다.