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http://dx.doi.org/10.7732/kjpr.2012.25.2.277

Adventitious Shoot Formation and Plant Regeneration from Explants of Solanum nigrum L.  

Oh, Soon-Ja (Institute for Field Science Education and Research)
Koh, Seok-Chan (Department of Biology, Jeju National University)
Publication Information
Korean Journal of Plant Resources / v.25, no.2, 2012 , pp. 277-284 More about this Journal
Abstract
In the present study, the effects of plant growth regulators on adventitious shoot and root formation of various explants of $in$ $vitro$ seedlings of $Solanum$ $nigrum$ L. were investigated to determine the optimum conditions for the high-efficiency plant regeneration of this species. The formation of adventitious shoots was higher in leaf explants than in cotyledon, hypocotyl, or epicotyl explants at low concentrations (0.5~2.0 mg $L^{-1}$ ) of 6-benzylaminopurine (BAP). The number of adventitious shoots and the shoot length were also higher in both leaf and cotyledon explants. In particular, 2.0 mg $L^{-1}$ BAP was most effective for stimulating the induction and multiplication of adventitious shoots. In terms of root formation and root development from shoots that were separated from multiple shoots, indole butyric acid (IBA) and indole acetic acid (IAA) were more effective than ${\alpha}$-naphthalene acetic acid (NAA). The percentage of rooting as well as the number of roots per shoot (4.0), root length (7.82 cm), and shoot length (8.76 cm) was highest on MS media supplemented with 0.05 mg $L^{-1}$ IAA. Furthermore, 100% of the regenerated plantlets survived when transplanted to compost soil. These results suggest that leaf explants are the best source for the high-efficiency regeneration of $S.$ $nigrum$ L., and that 2.0 mg $L^{-1}$ BAP and 0.05 mg $L^{-1}$ IAA are the best conditions for shoot and root induction, respectively.
Keywords
Solanum nigrum L.; Adventitious shoots; Root formation; BAP (6-benzylaminopurine); IAA (indole acetic acid);
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Times Cited By KSCI : 3  (Citation Analysis)
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