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Polysaccharide Characteristics from Hot Water Extract of Aloe saponaria Callus  

Baek, Jin-Hong (KJM Aloe R&D Center)
Kim, Myung-Uk (Department of Bioengineering and Technology, Kangwon National University)
Kang, Tae-Su (Department od Biofood Science and Biotechnology, Chungbuk Provincial College of Science & Technology)
Hur, Won (Department of Bioengineering and Technology, Kangwon National University)
Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
Publication Information
KSBB Journal / v.24, no.1, 2009 , pp. 59-64 More about this Journal
Abstract
The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 ${\mu}M$ NAA (${\alpha}$-naphthalene acetic acid) and 3-7 ${\mu}M$ kinetin under incubation in the dark at $25^{\circ}C$ for 6 weeks. The hot water extract ($100^{\circ}C$, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.
Keywords
Aloe saponaria; callus culture; hot water extract; GPC; polysaccharide;
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