Browse > Article

Polysaccharide Characteristics from Hot Water Extract of Aloe saponaria Callus  

Baek, Jin-Hong (KJM Aloe R&D Center)
Kim, Myung-Uk (Department of Bioengineering and Technology, Kangwon National University)
Kang, Tae-Su (Department od Biofood Science and Biotechnology, Chungbuk Provincial College of Science & Technology)
Hur, Won (Department of Bioengineering and Technology, Kangwon National University)
Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
Publication Information
KSBB Journal / v.24, no.1, 2009 , pp. 59-64 More about this Journal
Abstract
The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 ${\mu}M$ NAA (${\alpha}$-naphthalene acetic acid) and 3-7 ${\mu}M$ kinetin under incubation in the dark at $25^{\circ}C$ for 6 weeks. The hot water extract ($100^{\circ}C$, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.
Keywords
Aloe saponaria; callus culture; hot water extract; GPC; polysaccharide;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Vanisree M. and H. S. Tsay (2004), Plant cell cultures-An altemative and efficient source for the production of biologically important secondary metabolites, lnternational Journal of Applied Science and Engineering 1, 29-48
2 Yu C. Y., I. S. Jeon, S. D. Ahn, D. H. Cho, and I. M. Choung (1994), Rapid micropropagation of Aloe arborescens Mill. by meristem culture, J. Oriental Bot. Res, 7(1), 17-22
3 Kawai K., H. Beppu, T. Koike, K. Fujita, and T. Marunuchi (1993), Tissue culture of Aloe arborescens Miller var. natoalensis Berger, Phytotherapy Research 7, 5-10   DOI
4 Sanchez I. C., Natali L., and A. Cavallini (1988), In vitro culture of Aloe barbadensis Mill.: Morphogenic ability and nuclear DNA content, Plant Sci. 55, 53-59   DOI   ScienceOn
5 Gunter E. A. and Yu S. Ovodov (2002), Changes in cell wall polysaccharides of Silene vulgaris callus during culture, Phytochemistry 59, 703-708   DOI   ScienceOn
6 Bushneva O. A., R. G. Ovodova, A. S. Shashkov, A. O. Chizhov, Günter E. A., and Yu S. Ovodov (2006), Structural studies of arabinigalactan and pectin from Silene vulgaris (M.) G. callus, Bochemistry (Moscow) 71(6), 644-651   DOI
7 Yagi A., S. J. Shibata, J. Nishioka, S. Iwadare, and Y. Ishida (1982), Cardiac stimulant action of constituents of Aloe saponaria. J. Pharmaceut. Sci. 71(7), 739-741   DOI
8 AOAC. (1995), Official methods of analysis. 16th(ed). Association of Official Analytical Chemists, Washington D.C., USA
9 Femenia A., E. S. Sanchez, S. Simal, and C. Rossello (1999), Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues, Carbohydr. Polym. 39, 109-117   DOI   ScienceOn
10 Chauser-Volfson E. and Y. Gutierman (2004), Using Aloe plants growing in Israel as a source for the cosmetic and medicinal industries and as ornamental plants, Biochemical Systematics and Ecology 28, 825-828   DOI   ScienceOn
11 McAnalley, B. H. and G. Prairie (1990), Process for preparation of aloe products. US patent 4,957,907
12 Murashige, T. and F. Skoog (1962), A revised medium for rapid growth and bioassay with tobacco tissue culture, Physiologia Plantarum 15, 473-497   DOI
13 Konishi T. and M. Amano (1985), Callus formation in stamens of Aloe bellatura. Ann. Tsukuba Bot. Garden. 3, 19-26
14 Baksha R., M. A. A. Jahan, R. Khatun, and J. L. Munshi (2005), Micropropagation of Aloe barbadensis Mill. through in vitro culture of shoot tip explants, Plant Tissue Cult. & Biotech. 15(2), 121-126
15 Cho K. H. and J. H. Lee (1998), Preparation process of anthraquinone by culturing Aloe saponaria, Korea Patent No. 10-0148313-0000
16 Gunter E. A., and Yu S. Ovodov (2003), Production of polysaccharides by Silene vulgaris callus culture of depending on carbohydrates of the medium, Bochemistry (Moscow) 68(8), 882-889   DOI
17 Meyer HJ, and J. van Staden (1991). Rapid in vitro propagation of Aloe barbadensis Mill., Plant Cell Tissue and Organ Culture 26, 167-171   DOI
18 Talmadge K. W., K. Keegstra, W. D. Bauer, and P. Albersheim (1973), The structure of plant cell walls. 1. The macromolecular components of the walls of suspension-cultured sycamore cells with a detailed analysis of the pectic polysaccharides, Plant Physiol. 51, 158-173   DOI   ScienceOn
19 Leung M. Y. K., C. Liu, J. C. M. Koon, and K. P. Fung (2006), Polysaccharide biological response modifiers, lmmunology Letters 105, 101-114   DOI   ScienceOn
20 Abrie A. L. and J. van Staden (2001), Micropropagation of the endangered Aloe polyphylla, Plant Growth Regulation 33, 19-23   DOI   ScienceOn
21 Nancy K. Brown (NKB) Aesthetic, Inc., 26 May, 2008
22 Esua M F. and J. W. Rauwald (2006), Novel bioactive rnaloyl glucans from Aloe vera gel: Isolation, structure elucidation and in vitro bioassays, Carbohydrate Research 341, 355-364   DOI   ScienceOn
23 Gunter E. A, O. V. Popeiko, and Yu S. Ovodov (2007), Modification of polysaccharides from callus culture of Silene vulgaris (M.) G. using carbohydrases in vitro. Bochemistry (Moscow) 72(9), 1008-1015   DOI
24 Gowda, D., B. Neelisiddaiah, and Y. Anjaneyalo (1980), Structural studies of polysaccharides from Aloe saponaria and Aloe vanbalenii, Carbohydrate Res. 83, 402-405   DOI   ScienceOn
25 Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951), Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193, 265-275   PUBMED
26 Gunter E. A. and Yu S. Ovodov (2002), An altemate carbon source for enhancing production of polysaccharides by Silene vulgaris callus, Carbohydrate Research 337, 1641-1645   DOI   ScienceOn
27 Reynolds, T. and A. C. Dweck (1999), Aloe vera leaf gel: a review update, J. Ethnopharmacol. 68, 3-37   DOI   ScienceOn
28 Natali, L., I. C. Sanchez, and A. Cavallini (1990), In vitro culture of Aloe barbadensis Mill. Micropropagation from vegetative meristems, Plant Cell Tissue and Organ Culture 20, 71-74   DOI
29 Dubois M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith (1956), Colorimetric method for determination of sugars and related substances, Anal. Chem. 28, 350-356   DOI
30 Aggarwal D. and K. Bama (2004), Tissue culture propagation of elite plant of Aloe vera Linn., J Plant Biochemistry & Biotechnology 13, 77-79   DOI   ScienceOn
31 Chaudhuri S. and U. Mukundan (2001), Aloe barbadensis L. micropropagation and characterization of its gel, Phytomorphology 51(2), 155-157
32 Yagi A , Y. Shoyama, and I. Nishioka (1983), Formation of tetrahydroanthracene glycosidεs by callus tissue of Aloe saponaria, Phytochemistry 22, 1483-1484   DOI   ScienceOn
33 Sampedroa M. C., R. L. Artolab, M. Muraturec, D. Muratureb, Y. Ditamoa, G. A Rotha, and S. Kivatinitza (2004), Mannan from Aloe saponaria inhibits tumoral cell activation and proliferation, International Immunopharmacology 4, 411-418   DOI   ScienceOn
34 Chukwujekwu J. C., C. W. Fennell, and J. van Staden (2002), Optimization of the tissue culture protocol for the endangered Aloe polyphylla, South Afr. J. Botany 68, 424-429   DOI
35 Gunter E. A., O. V. Popeiko, and Yu S. Ovodov (2004), Isolation of polysaccharides from the callus culture of Lemna minor L., Applied Biochemistry and Mìcrobiology 40(1), 80-83   DOI   ScienceOn
36 Talmadge J, J. Chavez， L. Jacobs, C. Munger, T. Chinnah, J. T. Chow, D. Williamson, and K. Yates (2004), Fractionation of Aloe vera L. inner gel, purification and molecular profiling of activity, lnternational lmmunipharmacology 4, 1757-1773