• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.5
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• pp.94-99
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• 2004

This study was carried out to find out what is the optimum conditions for RAPD of Zelkova serata. We changes the factors what affect to PCR band patterns, as a result, we established the optimum conditions as follows; template DNA 100mg, Primer 0.25uM, dNTP 100mM, Taq polymerase 1.0u, and total reaction volume was filled up to 10uL with distilled water. As the amount of primers went higher, PCR reaction rates were lowered. This reason was cause by exhaustion of primers during initial reaction. The amount of dNTP didn't showed noticable differtations between the range, but the optimum amount was 100mM for efficiency. Taq polymerase 1.0 unit was the best in the range. As the concentration of polymerase were increased, many non-specific bands were appeared, In primer selection, most Openron Random Primers are amplified in this experiment. The primers GC contents were 60, and set A, B, C, D, E, X were tested. Thermal cycler(ASTEC PC808, Japan) condition was, $95^{\circ}C$, 5min, initial denaturation, $94^{\circ}C$, 20sec, denaturation, $37^{\circ}C$, 40sec, annealing, $72^{\circ}C$, 1min, extention, 45cycle repeated and final extention $72^{\circ}C$10min.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.1
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• pp.147-153
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• 2015

Since the DoS(Denial of Service) attack is still an important vulnerable element in many web service sites, sites including public institution should try their best in constructing defensive systems. Recently, DDoS(Distributed Denial of Service) has been raised by prompting mass network traffic that uses NTP's monlist function or DoS attack has been made related to the DNS infrastructure which is impossible for direct defense. For instance, in June 2013, there has been an outbreak of an infringement accident where Computing and Information Agency was the target. There was a DNS application DoS attack which made the public institution's Information System impossible to run its normal services. Like this, since there is a high possibility in having an extensive damage due to the characteristics of DDoS in attacking unspecific information service and not being limited to a particular information system, efforts have to be made in order to minimize cyber threats. This thesis proposes a method for using TTL (Time To Live) value in IP header to detect DDoS attack with IP spoofing, which occurs when data is transmitted under the agreed regulation between the international and domestic information system.

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.455-460
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• 1986

Dane particle was prepared from the plasma of chronic HBsAg carrier with high levels of HBsAg activity. DNA extracted front Dane particle core after a DNA polymerase reaction with $\alpha$-($^{32}$P) dNTP, was identified as HBV DNA by liquid scintillation counter and agarose gel electrophoresis-G.M. counting. To produce Hepatitis B surface antigen for use as a vaccine against Hepatitis B virus infection, yeast strains harboring recombinant plasmid with Apase promoter was used. Recombinant plasmid was construced from pHBV 130 and pAN 82, transformed into E coli, and then transferred into yeast strains. HBsAg was produced by derepression in Burkholder minimal medium with controlled inorganic phosphate concentration. The kinetics of HBsAg production was also investigated. Total HBsAg activity increased rapidly between 3 and 6 hours after transfer to phosphate-free medium and reached a maximum at around 9th hour. The transfer into phosphate-free medium after 6 hours in high phosphate cell growth medium gave maximum activity.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.18 no.5
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• pp.161-169
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• 2018

This study presents a time synchronization system in decentralized structure by using the blockchain, a core technology of Bitcoin introduced by Satoshi Nakamoto in 2008. In this study, Getting away from existing time synchronization system in centralized structure, A blockchain network has completely decentralized structure using public blockchain. In decentralized structure, Only certain peers among the peers that participate in a blockchain network access the NTP server. Therefore, others can synchronize time without having to go to public network. Furthermore if appropriate time synchronization cycles are established for each peer, time synchronization can be maintained even when connection to public network is completely lost. A time synchronization system in this study has advantages of p2p system and can be also guaranteed reliability and stability because it used digital signature, merkle tree, consensus algorithm which are core characteristics of block chains.

• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.7-12
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• 1996

Reproducible the random amplified polymorphic DNAs(RAPDs) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200$\mu$M), MgCl2(1.5mM) and template DNA(30ng). In addition, anealing temperature ranging 35$^{\circ}C$ to 42$^{\circ}C$ by the adjusted condition was investigated and fixed at 35$^{\circ}C$ in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorhpisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorhpims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDs detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori

• KSBB Journal
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• v.17 no.4
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• pp.326-332
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• 2002

The quality of biopharmaceutical proteins is strongly affected by a manufacturing process employed to produce Et, and thus validation of the manufacturing bioprocess is a very important issue. Chromatography is probably the most widely used bioprocess unit operation for protein purification. In this study, a miniaturized automatic chromatography system was designed and constructed for scale-down studies for process chromatography validation. This system, named MiniValChrom, has the following features: automatic and repeated operation, flexible sequences and intervals among the steps, on-line and real-time monitoring and control, method files savings, etc. Using the MiniValChrom, we peformed a case study of an abbreviated experiment to estimate chromatographic resin lifetime. BSA (bovine serum albumin) and Cibacron Blue 3G-A were used as the model protein and the resin, respectively. The resin deterioration was evaluated by determining and monitoring the HETP and NTP values from the chromatograms every 5 cycles. It was observed that the HETP and the NTP values were changed by 9% after 15 cycles. The resin lifetime validation could be completed by repeating this experiment until the HETP value reached a predetermined value. The MiniValChrom's concept and the protocol suggested in this study can serve as a rapid and economical tool for the validation studies of bioprocess chromatography system.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.12B
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• pp.1611-1622
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• 2011

The synchronization is one of the important issues for successful operation of femtocell. The synchronization of femtocell is distinctly different from that of larger wireless base stations in a number of important respects such as 1) The femtocell is located in indoor environment which may make it difficult to receive the adequate GNSS signals. 2) The backhaul of femtocell is connected to the public network which may have more PDV than private network. 3) The entire cost of femtocell needs to be very low. In our thesis, we investigate the candidate solutions including AGNSS (Assisted GNSS), NTP (Network Time Protocol), PTP (Precision Timing Protocol) and Cellular Network Listen for indoor timing solution. We propose the AGNSS-PTP Hybrid scheme which can improve time and frequency quality by selecting the better reference between AGNSS and PTP, and cover the standard status which are under discussion from IEEE, ITU-T, and IETF.

• The Korean Journal of Mycology
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• v.20 no.4
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• pp.315-323
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• 1992

The effects of several parameters on PCR amplification in using RAPD were studied. The results of this study suggest that approximately 15 ng of genomic DNA in $20\;{\mu}l$ of reaction mixture results in discrete and reproducible PCR products. In addition, the results indicate that concentration or amounts of reaction components studied are highly inter-dependent in their effects, and RNA can interfere severely with PCR amplification. Suitable concentrations or amounts of reaction components were found to be 30 ng of 10-mer primer, $200\;{\mu}M$ of dNTP, 0.001% gelatin 1.5 mM $MgCl_2$, 10 mM Tris-Cl (pH 8.8), 50 mM KCl, 0.1% Triton X-100, 2 units of Taq DNA polymerase, and 15 ng of RNase-treated genomic DNA in $25\;{\mu}l$ of reaction mixture.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.935-940
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• 2005

Fluorescence change of coumarin labeled phosphate binding protein (PBP-MDCC) was monitored to measure the amount of released inorganic phosphate ($P_{i}$) during nucleoside triphosphate (NTP) hydrolysis reaction. After purification of PBP-MDCC, fluorescence emission spectra showed that fluorescence responded linearly to $P_{i}$ up to about 0.7 molar ratio of $P_{i}$ to protein. The correlation of fluorescence signal and $P_{i}$ standard was measured to obtain [$P_{i}$] - fluorescence intensity standard curve on the stopped-flow instrument. When T7 bacteriophage helicase, double-stranded DNA unwinding enzyme using dTTP hydrolysis as an energy source, reacted with dTTP, the change of fluorescence was able to be converted to the amount of released $P_{i}$ by the $P_{i}$ standard curve. $P_{i}$ release results showed that single-stranded Ml3 DNA stimulated dTTP hydrolysis reaction several folds by T7 helicase. Instead of end point assay in NTP hydrolysis reaction, real time $P_{i}$-release assay by PBP-MDCC was proven to be very easy and convenient to measure released $P_{i}$.

• Journal of Convergence for Information Technology
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• v.10 no.12
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• pp.22-30
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• 2020

With the development of information and communication technology, DDoS and DRDoS continue to become security issues, and gradually develop into advanced techniques. Recently, IT companies have been threatened with DRDoS technology, which uses protocols from normal servers to exploit as reflective servers. Reflective traffic is traffic from normal servers, making it difficult to distinguish from security equipment and amplified to a maximum of Tbps in real-life cases. In this paper, after comparing and analyzing the DNS amplification and Memcached amplification used in DRDoS attacks, a countermeasure that can reduce the effectiveness of the attack is proposed. Protocols used as reflective traffic include TCP and UDP, and NTP, DNS, and Memcached. Comparing and analyzing DNS protocols and Memcached protocols with higher response sizes of reflective traffic among the protocols used as reflective traffic, Memcached protocols amplify ±21% more than DNS protocols. The countermeasure can reduce the effectiveness of an attack by using the Memcached Protocol's memory initialization command. In future studies, various security-prone servers can be shared over security networks to predict the fundamental blocking effect.