• 한국독성학회:학술대회논문집
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• 한국독성학회 2000년도 국제심포지움 및 추계학술대회
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• pp.31-41
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• 2000

The major issue in the post genome sequencing era is determination of gene expression patterns in variety of biological systems. A microarray system is a powerful technology for analyzing the expression profile of thousands of genes at one experiment. In this study, we constructed cDNA microarray which carries 2,304 cDNAS derived from oligo-capped mouse cDNA library. Using this hand-made microarray we determined gene expression in various biological systems. To determine tissue specific genes, we compared Nine genes were highly-expressed in adult mouse brain compared to kidney, liver, and skeletal muscle. Tissue distribution analysis using DNA microarray extracted 9 genes that were predominantly expressed in the brain. A database search showed that five of the 9 genes, MBP, SC1, HiAT3, S100 protein-beta, and SNAP25, were previously known to be expressed at high level in the brain and in the nervous system. One gene was highly sequence similar to rat S-Rex-s/human NSP-C, suggesting that the gene is a mouse homologue. The remaining three genes did not match to known genes in the GenBank/EMBL database, indicating that these are novel genes highly-expressed in the brain. Our DNA microarray was also used to detect differentiation specific genes, hormone dependent genes, and transcription-factor-induced genes. We conclude that DNA microarray is an excellent tool for identifying differentially expressed genes.

• 한국경영과학회지
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• 제30권3호
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• pp.17-39
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• 2005

The coordination of a three-level supply chain consisting of a supplier, a retailer, and a discount outlet (DCO) is studied here. We assume that the product is sold in two consecutive periods a Normal Sales Period (NSP) and a subsequent Clearance Salvage Period (CSP). A benchmark case is studied Initially in which the supply chain is coordinated by a s1n91e agent. Thus, the supplier the retailer, and the discount outlet design a common system that allows centralized decision making about stocking quantities, markdown time schedules, and policies on disposing of leftovers to deliver the greatest possible expected supply chain profit. Next, we consider a decentralized supply chain. Here, decisions are made without coordination. The objective is to maximize an individual party's expected profits. The focus of the study is on the following questions: what factors make the coordination an effective approach for the supply chain? How do we coordinate the supply chain so as to maximize the supply chain Joint expected profit? These and other related study issues are explored in this paper.

• 한국항해항만학회지
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• 제43권6호
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• pp.353-360
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• 2019

Technology-driven development of a new system makes it difficult for users and stakeholders to identify or intervene in the development process, resulting in systems with unnecessary functions and poor quality services. Applying the software architecture design process to the initial design of the navigation system platform of autonomous ships enables the development of a system that reflects the required functions and service quality of the stakeholders. The design, which includes all of the subsystems that make up an autonomous ship platform, is close to an enterprise architecture. Thus, we strived to design a navigation system platform suitable for the design range of the software architecture. This study analyzed the definition of functional requirements, and quality attributes by applying the software architecture design procedure. This study was conducted to identify the characteristics of the navigation system and platform needs, and the stakeholders were identified. To derive the functional requirements and constraints of the platform, a quality attributes workshop was held engaging stakeholders, and the results of the analysis of functional requirements and quality attributes were listed. Based on the results of this study, the architect can establish the evidence and technical solutions that are integral for the architecture development, and will facilitate the creation of quality attribute scenarios.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1586-1589
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• 2005

GUMF (Global User Management Framework) that is proposed in this research can be applied to next generation network such as BcN (Broadband convergence Network), it is QoS guaranteed security framework for user that can solve present Internet's security vulnerability. GUMF offers anonymity for user of service and use the user's real-name or ID for management of service and it is technology that can realize secure QoS. GUMF needs management framework, UMS (User Management System), VNC (Virtual Network Controller) etc. UMS consists of root UMS in country dimension and Local UMS in each site dimension. VNC is network security equipment including VPN, QoS and security functions etc., and it achieves the QoSS (Quality of Security Service) and CLS(Communication Level Switching) functions. GUMF can offer safety in bandwidth consumption attacks such as worm propagation and DoS/DDoS, IP spoofing attack, and current most attack such as abusing of private information because it can offer the different QoS guaranteed network according to user's grades. User's grades are divided by 4 levels from Level 0 to Level 3, and user's security service level is decided according to level of the private information. Level 3 users that offer bio-information can receive secure network service that privacy is guaranteed. Therefore, GUMF that is proposed in this research can offer profit model to ISP and NSP, and can be utilized by strategy for secure u-Korea realization.

• Management Science and Financial Engineering
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• 제11권1호
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• pp.1-24
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• 2005

The results of a study of the coordination effect in stocking and promotional markdown policies for a supply chain consisting of a retailer and a discount outlet (DCO) are reported here. We assume that the product is sold in two consecutive periods: the Normal Sales Period (NSP) and the subsequent Promotional Markdown Sales Period (PSP). We first study an integrated supply chain in which managers in the two periods design a common system so as to jointly decide the stocking quantities, markdown time schedule, and markdown price to maximize mutual profit. Next, we consider a decentralized supply chain. An uncoordinated contract is designed in which decisions are decentralized to optimize the individual party's objective function. Here, three sources of system inefficiencies cause the decentralized system to earn a lower expected system profit than that in the integrated supply chain. The three sources are as follows: in the decentralized system the retailer tends to (1) stock less, and (2) keep a longer sales period, and the DCO tends to (3) stock fewer leftovers inventories and charge a higher markdown price. Finally, a numerical experiment is provided to compare the coordinated model with the uncoordinated model to explore factors that make coordination an effective approach.

• Earthquakes and Structures
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• 제9권3호
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• pp.673-698
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• 2015

This paper investigates inelastic seismic demands of the normal component of near-fault pulse-like ground motions, which differ considerably from those of far-fault ground motions and also parallel component of near-fault ones. The results are utilized to improve the nonlinear static procedure (NSP) called Displacement Coefficient Method (DCM). 96 near-fault and 20 far-fault ground motions and the responses of various single degree of freedom (SDOF) systems constitute the dataset. Nonlinear Dynamic Analysis (NDA) is utilized as the benchmark for comparison with nonlinear static analysis results. Considerable influences of different faulting mechanisms are observed on inelastic seismic demands. The demands are functions of the strength ratio and also the pulse period to structural period ratio. Simple mathematical expressions are developed to consider the effects of near-fault motion and fault type on nonlinear responses. Modifications are presented for the DCM by introducing a near-fault modification factor, $C_N$. In locations, where the fault type is known, the modifications proposed in this paper help to obtain a more precise estimate of seismic demands in structures.

• The Plant Pathology Journal
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• 제38권3호
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• pp.254-260
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• 2022

Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.

• Journal of Veterinary Science
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• 제21권4호
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• pp.68.1-68.8
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• 2020

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

• Asian-Australasian Journal of Animal Sciences
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• 제26권1호
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• pp.97-107
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• 2013

The aim of this study was to establish relationships between chemical and physical parameters of wheat with performance and digestibilities of feed components in broiler chickens fed on wheat-based diets. Ninety-four wheat samples were selected for inclusion in four bird trials. Birds were housed in individual wire metabolism cages from 7 to 28 d and offered water and feed ad libitum. Dry matter intake (DMI), liveweight gain (LWG) and gain:feed were measured weekly. A balance collection was carried out from 14 to 21 d for determination of apparent metabolizable energy (AME), ME:gain, dry matter retention, oil and neutral detergent fibre (NDF) digestibility. At 28 d the birds were humanely killed, the contents of the jejunum removed for determination of in vivo viscosity and the contents of the ileum removed for determination of ileal dry matter, starch and protein digestibility. When wheat parameters were correlated with bird performance data, it was found that specific weight was not significantly (p>0.05) related to bird performance. Bird DMI, LWG and gain:feed were best correlated (p<0.05) with the rate of starch digestion, although the coefficients of correlation (r) were still low (0.246 to 0.523). A negative relationship (p<0.01) between AME and total (r = -0.432) and soluble (r = -0.304) non starch polysaccharide (NSP) was observed in this study. Thousand grain weight (TG) was positively correlated with DMI (r = 0.299), LWG (r = 0.343) and gain:feed (r = 0.371). When establishing multiple regression relationships, correlation coefficients greater than 0.8 were achieved for DMI, LWG, gain:feed and ileal crude protein digestibility. However, the economics involved in determining the parameters involved in the regressions make the process impractical.

• 대한음성학회:학술대회논문집
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• 대한음성학회 1996년도 10월 학술대회지
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• pp.362-366
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• 1996

This paper is generally divided in 2 parts. One is the study on vowels about korean singer's lyric song in view of Daniel Jones' Cardinal Vowel. The other is acoustic study on vowels in my singing about korean lyric song. Analysis data are KBS concert video tape and CSL's. NSP file on my singing and Informants are famous singers i.e. 3 sopranos, 1 mezzo, 2 tenors, 1baritone, and me. Analysis aim is to find out Korean 8 vowels([equation omitted]) quality in singing. The methods of descrition are used in closed vowels, half closed vowels, half open vowels, open vowels and rounded vowels, unroundes vowels and formants. The study of the former is while watching the monitor screen to stop the scene that is to be analysixed. The study of the latter is to analysis the spectrogram converted by CSL's. SP file. Analysis results are an follows: Visual and auditory korean vowels quality in singing have the 3 tendency. One is the tendency of more rounded than is usual Korean vowels. Another is the tendency of centralized to center point in Cardinal Vowel and the other is the tendency of diversity in vowel quality. Acoustic analysis is studied by means of 4 formants. Fl and F2 show similiar step in spoken. In Fl there is the same formant values. This seems to vocal organization be perceived the singign situation. The width of F3 is the widest of all, so F3 may be the characteristics in singing. In conclude, the characteristics of vowels in Korean lyric songs are seems to have the tendencies of rounding, centralizing to center point in Cardinal Vowel, diversity in vowel quality and, F3'widest width in compared with usual Korean vowels.