• Title/Summary/Keyword: NPR analysis

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Isolation and characterization of the rice NPR1 promoter

  • Hwang, Seon-Hee;Hwang, Duk-Ju
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.29-35
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    • 2010
  • NPR1 is a positive regulator of systemic acquired resistance in Arabidopsis and rice. Expression of the rice gene OsNPR1 is induced by salicylic acid (SA). To identify the region of the OsNPR1 promoter involved in response to SA, we carried out deletion mutagenesis of the region 1005 bp upstream of the OsNPR1 start codon. Ciselement analysis revealed that the OsNPR1 promoter contains W-boxes and ASF1 motifs, both of which are known to be functional cis-elements of the WRKY and bZIP proteins, respectively. The deletion constructs 1005:LUC and 752:LUC, were induced by up to 4.3- and 3.8-fold, respectively, following SA treatment, suggesting that W-boxes and ASF1 motifs may play an important role in the strong induction of these constructs by SA. Using mutation analysis, we also showed that both the W-box and ASF1 motif are necessary for SA-induced expression of OsNPR1.

Cloning and mulecular characterization of a nprX gene of bacillus subtilis NS15-4 encoding a neutral protease (Cloning and Molecular Characterization of a nprX gene of Bacillus subtilis NS15-4 Encoding a Neutral protease)

  • Lee, Seung-Hwan;Yoon, Ki-Hong;Nam, Hee-Sop;Oh, Tae-Kwang;Lee, Seog-Jae;Chae, Keon-Sang
    • Journal of Microbiology
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    • v.34 no.1
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    • pp.68-73
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    • 1996
  • An nprX gene of Bacillus subtilis NS15-4 encoding a neutral protease was cloned and its molecular characteristics were analyzed. The complete nucleotide sequence indicated that there is an open reading frame (0RF) possibly encoding 521 amino acid polypeptide. The ORF used all codons expected two cysteine and a proline having a codon bias index (CBI) of 0.09 in Escherichia coli. There were homologous sequences to the consensus sequence of -35 and -10 regions of E. coli promoters and to a Shine-Dalgarno (SD) sequence located 25 bp downstream of a mojor transcription initiation site. Moreover, there were also five minor transcription initiation sites at 6. 7. 8. 14 and 15 nt downstream of the major site. Northern blot analysis revealed the presence of about 1.8 kb mRNA transcript in E. coli having the nprX gene. The nucleotide sequence was identified in GenBank to be a gene for a neutral protease of B. sutilis with six nucleotide difference in the ORF region. The flanking regions of the NprX ORF showed much more differences form those of other neutral protease genes except the nprE gene of B. subtilis, which has the most homology to the nprX gene, and of which the flanking regions were identical to those of the nprX gene.

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Experimental assessment on flexural behavior of demountable steel-UHPC composite slabs with a novel NPR steel plate

  • Jin-Ben Gu;Jun-Yan Wang;Yi Tao;Qing-Xuan Shi
    • Steel and Composite Structures
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    • v.49 no.4
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    • pp.381-392
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    • 2023
  • This study experimentally investigates the flexural behavior of steel-UHPC composite slabs composed of an innovative negative Poisson's ratio (NPR) steel plate and Ultra High Performance Concrete (UHPC) slab connected via demountable high-strength bolt shear connectors. Eight demountable composite slab specimens were fabricated and tested under traditional four-point bending method. The effects of loading histories (positive and negative bending moment), types of steel plate (NPR steel plate and Q355 steel plate) and spacings of high-strength bolts (150 mm, 200 mm and 250 mm) on the flexural behavior of demountable composite slab, including failure mode, load-deflection curve, interface relative slip, crack width and sectional strain distribution, were evaluated. The results revealed that under positive bending moment, the failure mode of composite slabs employing NPR steel plate was distinct from that with Q355 steel plate, which exhibited that part of high-strength bolts was cut off, part of pre-embedded padded extension nuts was pulled out, and UHPC collapsed due to instantaneous instability and etc. Besides, under the same spacing of high-strength bolts, NPR steel plate availably delayed and restrained the relative slip between steel plate and UHPC plate, thus significantly enhanced the cooperative deformation capacity, flexural stiffness and load capacity for composite slabs further. While under negative bending moment, NPR steel plate effectively improved the flexural capacity and deformation characteristics of composite slabs, but it has no obvious effect on the initial flexural stiffness of composite slabs. Meanwhile, the excellent crack-width control ability for UHPC endowed composite members with better durability. Furthermore, according to the sectional strain distribution analysis, due to the negative Poisson's ratio effect and high yield strength of NPR steel plate, the tensile strain between NPR steel plate and UHPC layer held strain compatibility during the whole loading process, and the magnitude of upward movement for sectional plastic neutral axis could be ignored with the increase of positive bending moment.

Analysis of the Oil painting for the painterly rendering -focusing on the 19C Impressionism painting- (회화적 렌더링 구현을 위한 유화 매체 분석 연구 -19C 인상주의 작품을 중심으로-)

  • Huh, Soo-Jung;Park, Jin-Wan
    • Proceedings of the Korea Contents Association Conference
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    • 2006.05a
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    • pp.259-264
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    • 2006
  • Some appearances of the result images from the researches on NPR(Non Photorealistic Rendering) look like contrary to the attributes of the real painting. As they are based on only the technical approach rather than the aesthetic and accurate analysis of the real painting which they modeled. Therefore the purpose of this paper is the abstraction of the exact features from the related real painting, the redefine of them applicable to the process, and the embodiment of the painterly NPR algorithms. This paper modeled the Impressionism which were originated in the France at the late nineteenth century. Accordingly, at first, I analyzed the general features of oil paintings and Impressionism paintings, and according to this analyses, I adjusted them to engineering elements(the direction, length, width, texture and speed of stroke, the edge and region of object, depth information and color etc) and programmed. I emphasize the importance and potentiality of the collaboration of artist and technician in the NPR research through the results in this paper.

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The Analysis on dominant cause of Process Failure in TFT Fabrication (박막트랜지스터 제조에서 공정실패 요인 분석)

  • Hur, Chang-Wu
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2007.06a
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    • pp.507-509
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    • 2007
  • 본 연구는 기존의 방식으로 만든 비정질 실리콘 박막 트랜지스터의 제조공정에서 발생되는 결함에 대한 원인을 분석하고 해결함으로써 수율을 증대시키고 신뢰성을 개선하고자한다. 본 연구의 수소화 된 비정질 실리콘 박막 트랜지스터는 Inverted Staggered 형태로 게이트 전극이 하부에 있다. 실험 방법은 게이트전극, 절연층, 전도층, 에치스토퍼 및 포토레지스터층을 연속 증착한다. 스토퍼층을 게이트 전극의 패턴으로 남기고, 그 위에 $n^+a-Si:H$ 층 및 NPR(Negative Photo Resister)을 형성시킨다. 상부 게이트 전극과 반대의 패턴으로 NPR층을 패터닝하여 그것을 마스크로 상부 $n^+a-Si:H$ 층을 식각하고, 남아있는 NPR층을 제거한다. 그 위에 Cr층을 증착한 후 패터닝하여 소오스-드레인 전극을 위한 Cr층을 형성시켜 박막 트랜지스터를 제조한다. 이렇게 제조한 박막 트랜지스터에서 생기는 문제는 주로 광식각공정시 PR의 잔존이나 세척 시 얇은 화학막이 표면에 남거나 생겨서 발생되며, 이는 소자를 파괴시키는 주된 원인이 된다. 그러므로 이를 개선하기 위하여 ashing 이나 세척공정을 보다 엄격하게 수행하였다. 이와 같이 공정에 보다 엄격한 기준의 세척과 여분의 처리공정을 가하여 수율을 확실히 개선 할 수 있었다.

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Design Factor Analysis of Aerospike Pintle Nozzle for Increasing Thrust in Off-Design (탈설계 조건에서 추력 증대를 위한 에어로 스파이크 핀틀 노즐의 설계인자 분석 연구)

  • Kim, Jeongjin
    • Journal of Aerospace System Engineering
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    • v.16 no.4
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    • pp.1-9
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    • 2022
  • A design factor analysis was conducted to reduce the thrust reduction in the off-design, due to the driving of the aerospike pintle nozzle. The close (NPR 100) as well as the open (NPR 11) stroke were fixed, as under-expansion conditions. The pintle contour, pintle head radius (R), cowl angle (θ), and cowl exit length (L) were selected as design factors. The change in thrust was analyzed, using a verified numerical analysis technique. First, the pintle head radius and the length of the cowl exit had little influence on the thrust. The cowl angle changed the mass flow rate by affecting the effective nozzle throat area, and created a reverse pressure gradient at the cowl exit. As a result of applying the dual aerospike contour, it was confirmed that the thrust in the design-off increased by approximately 1.2%, compared to the reference case and by approximately 3.4% compared to the worst case.

Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

  • Ghosh, M.K.;Das, B.K.;Das, C.;Mishra, A.K.;Mukherjee, P.K.;Urs, S.Raje
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.67-71
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    • 2004
  • Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year, The nitrate reductase (EC No. 1.6.6.1) activity (NRA, $\mu$mol N $O_2$- $h^{-1}$ $g^{-1}$ fr, wt.), total soluble protein (mg $g^{-1}$ fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area ($\textrm{cm}^2$), specific leaf weight (g c $m^{-2}$ ), total soluble sugar (mg $g^{-1}$ fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, $\mu$$m^{2}$ $s^{-1}$ ) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 $\mu$㏖ N $O_2$- $h^{-l}$ $g^{-1}$ fr, wt.), total soluble protein (39.63mg $g^{-1}$ fr, wt.), NPR(16.66 $\mu$$m^{-2}$ $s^{-1}$ ), total soluble sugar (48.44 mg $g^{-1}$ fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA af unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.t.

An Efficient Design Method of Linear-Phase Prototype Lowpass Filter for Near-Perfect Reconstruction Pseudo-QMF Banks (근접 완전재생 Pseudo-QMF 뱅크를 위한 선형위상 프로토타입 저역통과 필터의 효율적인 설계 방법)

  • Jeon, Joon-Hyeon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.3C
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    • pp.271-280
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    • 2008
  • M channel near-perfect-reconstruction(NPR) pseudo-QMF banks are a hybrid of conventional pseudo-QMF design and spectral factorization approach where the analysis and synthesis filters are cosine-modulated versions of the prototype-lowpass filter(p-LPF). However, p-LPF H(z) does not have linear-phase symmetry as well as magnitude-distortion optimization since it is obtained by spectral factorization of $2M^{-th}$ band filter $G(z)=z^{-(N-1)}H(z^{-1})H(z)$. A fair amount of attention, therefore, has been focused on the design of filter banks for reducing only alias-cancellation distortion without reconstructed-amplitude distortion. In this paper, we propose a new method for designing linear-phase p-LPF in NPR pseudo-QMF banks, which is based on Maxflat(maximally flat) FIR filters with closed-form transfer function. In addition, p-LPF H(z) is optimized in this approach so that the 2M-channel overall distortion response represented with $G(z)=H^2(z)$ approximately becomes an unit magnitude response. Through several examples of NPR pseudo-QMF banks, it is shown that the peek ripple of the overall magnitude distortion is less than $3.5{\times}10^{-4}\;({\simeq}-70dB)$ and analysis/synthesis filters have the sharp monotone-stopband attenuation exceeding 100 dB.

Exploring the Key Technologies on Next Production Innovation (4차 산업혁명 차세대 생산혁신 기술 탐색: 키워드 네트워크를 중심으로)

  • Lee, Suchul;Ko, Mihyun
    • Journal of the Korea Convergence Society
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    • v.9 no.9
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    • pp.199-207
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    • 2018
  • This study aims to analyze Next Production Revolution (NPR) technologies through evidence-based keyword network in order to cope with the change of production paradigm called the Fourth Industrial Revolution (4IR). For the analysis, a total of 441 papers related to NPR or 4IR were extracted and the NPR technology network was constructed based on the simultaneous appearance relationship of the author keywords of these papers. Based on the NPR technology network, we explored key technologies through analysis of centrality and keyword group. As a result, technologies such as 'digital twin' and 'modeling and simulation', discovering insights by connecting the virtual and physical world in real time and reflecting them into design and process, are analyzed as key technologies.

Plume Structure Analysis of an Axisymmetric Supersonic Micro-nozzle at the Various Pressure Ratios (압력비가 변할 때 축대칭 초음속 노즐의 플룸 구조 해석)

  • Kwon, Soon-Duk;Kim, Sung-Cho;Kim, Jeong-Soo;Choi, Jong-Wook;Kim, Yong-Sseok
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2862-2867
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    • 2007
  • The steady non-reacted compressible flow field in a symmetric micro-thruster, which is used for the accurate attitude control of a satellite, is analyzed varying the nozzle pressure ratio (NPR) to investigate the plume characteristics. The nozzle throat diameter is 0.06 inch and the area ratio is 56. The recirculation region is found just behind the normal shock at the several NPRs due to the locally adverse pressure gradient along the nozzle centerline when the environmental pressure is atmospheric. This phenomenon, the cause of flow loss, is similar to the flow behind a blunt body. As NPR increases the location of Mach disk, characteristics of the normal shock, moves downstream and its strength increases. The Mach number distribution appears in a wave-type patter after the normal shock because oblique shocks are reflected on the shock boundaries especially when NPRs are very high.

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