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http://dx.doi.org/10.1007/s11816-009-0116-5

Isolation and characterization of the rice NPR1 promoter  

Hwang, Seon-Hee (National Academy of Agricultural Sciences, Rural Development Administration)
Hwang, Duk-Ju (National Academy of Agricultural Sciences, Rural Development Administration)
Publication Information
Plant Biotechnology Reports / v.4, no.1, 2010 , pp. 29-35 More about this Journal
Abstract
NPR1 is a positive regulator of systemic acquired resistance in Arabidopsis and rice. Expression of the rice gene OsNPR1 is induced by salicylic acid (SA). To identify the region of the OsNPR1 promoter involved in response to SA, we carried out deletion mutagenesis of the region 1005 bp upstream of the OsNPR1 start codon. Ciselement analysis revealed that the OsNPR1 promoter contains W-boxes and ASF1 motifs, both of which are known to be functional cis-elements of the WRKY and bZIP proteins, respectively. The deletion constructs 1005:LUC and 752:LUC, were induced by up to 4.3- and 3.8-fold, respectively, following SA treatment, suggesting that W-boxes and ASF1 motifs may play an important role in the strong induction of these constructs by SA. Using mutation analysis, we also showed that both the W-box and ASF1 motif are necessary for SA-induced expression of OsNPR1.
Keywords
cis-Elements; OsNPR1 promoter; Salicylic acid; WRKY transcription factor;
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1 Chen C, Chen Z (2002) Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol 129:706-716   DOI   ScienceOn
2 Zhang Y, Fan W, Kinkema M, Li X, Dong X (1999) Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc Natl Acad Sci USA 96:6523-6528   DOI   ScienceOn
3 Shinshi H, Usami S, Ohme-Takagi M (1995) Identification of an ethylene-responsive region in the promoter of a tobacco class I chitinase gene. Plant Mol Biol 27:923-932   DOI   ScienceOn
4 Silverman P, Sesker M, Kanter D, Schweizer P, Metraux J-P, Raskin I (1995) Salicylic acid: biosynthesis, conjugation, in rice and possible role. Plant Physiol 108:633-639   DOI
5 Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK (2006) Overexpression of the pepper CARAV1 pathogen-induced gene encoding a RAV transcription factor induces pathogenesisrelated genes and enhances resistance to bacterial pathogen in Arabidopsis. Plant Mol Biol 61:897-915   DOI   ScienceOn
6 Pieterse CM, van Loon LC (2004) The spider in the web of induced resistance signaling pathways. Curr Opin Plant Biol 7:456-464   DOI   ScienceOn
7 Ryals J, Weymann K, Lawton K, Friedrich L, Ellis D, Steiner H-Y, Johnson J, Delaney TP, Jesse T, Vos P, Uknes S (1997) The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor $I{\kappa}B$. Plant Cell 9:425-439
8 Kagaya Y, Ohmiya K, Hattori T (1999) RAV1 a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants. Nucleic Acids Res 27:470-478   DOI   ScienceOn
9 Maleck K, Levine A, Eulgem T, Morgan A, Jurg S, Lawton KA, Dangle JL, Dietrich RA (2000) The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26:403-409   DOI   ScienceOn
10 Mou Z, Fan W, Dong X (2003) Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell 113:935-944   DOI   ScienceOn
11 Kinkema M, Fan W, Dong X (2000) Nuclear localization of NPR1 is required for activation of PR gene expression. Plant Cell 12:2339-2350   DOI
12 Lee SC, Hwang BK (2006) Identification and deletion analysis of the promoter of the pepper SAR8.2 gene activated by bacterial infection and abiotic stresses. Planta 224:255-267   DOI   ScienceOn
13 Fan W, Dong X (2002) In vivo interaction between NPR1 and transcription factor TGA2 leads to salicylic acid-mediated gene activation in Arabidopsis. Plant Cell 14:1377-1389   DOI   ScienceOn
14 Li Y-F, Zhu R, Xu P (2005) Activation of the gene promoter of barley $\beta$-1, 3-glucanase isoenzyme GIII is salicylic acid (SA)-dependent in transgenic rice plants. J Plant Res 118:215-221   DOI   ScienceOn
15 Despres C, DeLong C, Glaze S, Liu E, Fobert PR (2000) The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. Plant Cell 12:279-290   DOI
16 Eulgem T, Rushton PJ, Schmelzer E, Hahlbrock K, Somssich LE (1999) Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors. EMBO J 18:4689-4699   DOI   ScienceOn
17 Yamamoto S, Nakano T, Suzuki K, Shinshi H (2004) Elicitor-induced activation of transcription via W box-related cis-acting elements from a basic chitinase gene by WRKY transcription factors in tobacco. Biochim Biophys Acta 1679:279-287   DOI   ScienceOn
18 Cao H, Glazebrook J, Volko S, Dong X (1997) The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell 88:57-63   DOI   ScienceOn
19 Abe H, Yamaguchi-Shinizaki K, Urao T, Iwasaki T, Hosokawa D, Shinozaki K (1997) Role of Arabidopsis MYC and MYB homologs in drought- and Abscisic acid-regulated gene expression. Plant Cell 9:1859-1868
20 Wang D, Amornsiripanitch N, Dong X (2006) A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathogenet 2:1042-1050
21 Yang Y, Qi M, Mei C (2004) Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress. Plant J 40:909-919   DOI   ScienceOn
22 Yu D, Chen C, Chen Z (2001) Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell 13:1527-1539   DOI
23 Niggeweg R, Thurow C, Weigel R, Pfitzner U, Gatz C (2000) Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties. Plant Mol Biol 42:775-788   DOI   ScienceOn
24 Qiu D, Xiao J, Ding X, Xiong M, Cai M, Cao Y, Li X, Xu C, Wang S (2007) OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling. Mol Plant Microbe Interact 20:492-499   DOI   ScienceOn
25 Zhou JM, Trifa Y, Silva H, Pontier D, Lam E, Shah J, Klessig DF (2000) NPR1 differentially interacts with members of the TGA/ OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Mol Plant Microbe Interact 13:191-202   DOI   ScienceOn
26 Chern MS, Fitzgerald HA, Yadav RC, Canlas PE, Dong X, Ronald PC (2001) Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. Plant J 27:101-113   DOI   ScienceOn
27 Hwang S-H, Lee IA, Yie SW, Hwang DJ (2008) Identification of an OsPR10a promoter region responsive to salicylic acid. Planta 227:1141-1150   DOI   ScienceOn
28 Liu X, Bai X, Qian Q, Wang X, Chen M, Chu C (2005) OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Cell Res 15:593-603   DOI   ScienceOn