• 한국가금학회지
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• 제35권2호
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• pp.115-121
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• 2008

본 시험은 microbial phytase을 첨가하여 산란계에 급여시 산란율, 계란 품질, 인 이용율 및 영양소 소화율에 미치는 영향을 조사하기 위하여 수행하였다. 32주령 Hy-Line Brown 갈색계 120수를 공시하여, 4주간 사양시험을 실시하였다. 시험 설계는 CON(control), LP(low phosphorus diet), NP(low phosphorus diet + 0.03% normal microbial phytase) 및 CP(low phosphorus diet + 0.03% coated microbial phytase)로 4개 처리를 하여 처리당 5반복, 반복당 6수씩 완전 임의 배치하였다. 전체 기간 동안 산란율은 CON과 CP 처리구가 LP 처리구보다 높게 나타났다(P<0.05). 난각 강도와 난각 두께는 LP 처리구에 비해 CON, CP 및 NP 처리구가 높게 나타났다(P<0.05). 난황색과 haugh unit에서는 CON, CP 및 NP 처리구가 LP 처리구보다 높게 나타났다(P<0.05). 혈액내 칼슘과 인 농도는 CP 처리구가 LP 처리구에 비해 높게 나타났다(P<0.05). 건물 소화율과 질소 소화율에서는 CP 처리구가 CON, LP 및 NP 처리구에 비하여 유의적으로 증가하였다(P<0.05). 회분소화율은 CON, CP 및 NP 처리구가 LP 처리구에 비해 증가하였다(P<0.05). 칼슘 소화율과 인 소화율에서는 CP와 NP 처리구가 CON과 LP 처리구에 비해 높게 나타났다(P<0.05). 칼슘 흡수량은 CON, CP 및 NP 처리구가 LP 처리구보다 유의적으로 높게 나타났다(P<0.05). 인 흡수량은 CP와 NP 처리구가 CON과 LP 처리구와 비교하여 유의적으로 높게 나타내었다(P<0.05). 질소와 칼슘 배출량은 CON, NP 및 CP 처리구가 LP 처리구에 비해 유의적으로 낮게 나타냈다(P<0.05). 인 배출량은 LP, NP 및 CP 처리구가 CON 처리구에 비해 유의적으로 낮게 나타내었다(P<0.05). 결론적으로, 산란계에 있어 코팅된 microbial phytase의 첨가는 산란율, 계란 품질, 혈액 내 칼슘과 무기태 인, 영양소 소화율과 칼슘 및 인 흡수량을 증진시키며, 질소와 칼슘 배출량을 감소시키는 것으로 나타났다.

• 음성과학
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• 제9권4호
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• pp.187-200
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• 2002

The purpose of the present study is to see in terms of $F_{0}$ variation in Japanese how discourse focus and the lexical word accent interact with each other in realizing overall intonation patterns. Discourse focus causes prosodic restructuring of phrase structures and, as a result, largely affects pitch contours, whereas the lexical word accent is said to delimit the $F_{0}$ into a certain range. Measurement of $F_{0}$ was made of utterances of Japanese sentences to observe behavior of pitch contours with varied focus assignment and lexical accent specifications. The utterances were obtained in question-answer discourse contexts so that in a sentence, either one NP was always focused or no focus was assigned. I set four points for $F_{0}$ measurement; $F_{1s},F_{1m}, F_{2s}$, and $F_{2m}$, two for each noun phrase corresponding to $F_{0}$ at the beginning of the first syllable and that of the vocalic portion of the second syllable in the two NP's. The results of present study were as follows: (1) for all combination of lexical accent types, the $F_{0}$ rise both in NP1 and NP2 are higher when focused than when not focused. (2) NP2 starts a new accentual phrase when focused, showing even higher $F_{0}$ than NP1, the latter of which implies that in forming a new accentual phrase by focusing, catathesis does not seem to take effect on NP2 preceded by accented NP1. (3) unfocused NP2 preceded by unaccented NP1 has higher $F_{0}$ than those preceded by accented NP1.

• Animal cells and systems
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• 제14권3호
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• pp.161-167
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• 2010

This study investigated the estrogenicity of 4-nonylphenol (NP) and diethylstilbestrol (DES) in vitro during oocyte maturation in the marine fish, Tridentiger obscurus, using steroid hormone assays and GVBD assay. Vitellogenic (0.53mm diameter) and fully vitellogenic (0.75mm diameter) oocytes were in vitro exposed to NP (0.045 453.82 nM and DES (0.037 372.62 nM). In vitellogenic oocytes, 45.38 and 453.82nM NP and 3.73 372.62nM DES increas the estradiol-$17{\beta}$ (E2)/testosterone (T) ratio. In fully vitellogenic oocytes, 0.45, 45.38 and 453.82nM NP and 3.73nM DES increased E2/T. In the GVBD assay, 0.45 and 4.54nM NP and 0.037, 3.73 and 37.26nM DES inhibited GVBD. These results suggest that NP and DES have estrogen-agonistic effects in oocyte maturation in T. obscurus. In addition, NP and DES have different sensitivity according to the oocyte developmental stage, and the estrogenagonistic effects of DES were greater than were those of NP.

• Animal cells and systems
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• 제15권3호
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• pp.189-196
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• 2011

We investigated the in vitro effects of nonylphenol (NP) and 3,3',4,4',5-pentachlorobiphenyl (PCB126) on steroidogenesis in redlip mullet, Chelon haematocheilus, oocytes. In experiment 1, we investigated the effects of NP and PCB126 on steroid production from exogenous steroid precursors. Vitellogenic oocytes (0.75 mm in diameter) were incubated with 10 and 100 ng/ml NP or PCB126 with $[^3H]17{\alpha}$-hydroxyprogesterone as a precursor. The major metabolites produced were androstenedione, testosterone (T), estrone, and estradiol-$17{\beta}$ ($E_2$). Both NP and PCB126 increased T production and decreased $E_2$ production, except for 100 ng/ml PCB126. In experiment 2, oocytes (0.65-0.75 mm in diameter) were exposed to NP and PCB126 at different concentrations (0.01, 0.1, 1, 10, and 100 ng/mL). After the incubation, T and $E_2$ production was measured by radioimmunoassay. NP inhibited $E_2$ production at concentrations of 0.01 and 0.1 ng/ml in 0.75-mm-diameter oocytes. NP at 1 and 100 ng/mL stimulated T production, but had no observable effect on $E_2$ production. PCB126 treatment did not affect $E_2$ production at any of the concentrations tested. NP alone at 0.1 ng/mL resulted in a significant decrease in $E_2$ production in 0.65-mm-diameter oocytes. PCB126 did not show any significant effects on either T or $E_2$ production at all concentrations tested. These results suggest that NP acts like an antiestrogen at lower concentrations (0.01-0.1 ng/ml) in vitellogenic oocytes of redlip mullet.

• 한국축산식품학회지
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• 제36권6호
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• pp.729-736
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• 2016

This study was performed to evaluate the quality of fermented sausages manufactured with processed sulfur-fed pigs. The fermented sausages were divided into two groups; one was manufactured with non sulfur-fed pigs (NP), the other one was made with processed sulfur-fed pigs (SP). No differences were found in moisture and fat contents (p>0.05) between NP and SP, but the protein and ash con-tents of SP were significantly higher than those of NP (p<0.05). The pH of SP was significantly lower than that of NP, and the water activity ($a_w$) of SP was significantly higher than that of NP after 14 and 21 d (p<0.05). The TBA (Thiobarbituric acid reactive substance) w value of SP was significantly lower than that of NP (p<0.05). The lightness and yellowness of NP were significantly higher than those of SP, whereas the redness of NP was lower than SP (p<0.05). The total plate count of SP was lower than that of NP (p<0.05). There was no significant difference in TPA (Texture profile analysis) between the two samples. SP showed significantly increased monounsaturated fatty acid (p<0.05) and decreased saturated fatty acid. Umami taste and richness in SP were significantly higher than in NP (p<0.05). Therefore, it is suggested that processed sulfur fed pigs may play a key role in enhancing the quality of meat products.

• 한국발생생물학회지:발생과생식
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• 제23권2호
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• pp.93-99
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• 2019

Nonylphenols (NPs) are widely used industrial materials, and are considered as potent endocrine disrupting chemical. Present study was undertaken to clarify the effect of subchronic low-dose NP exposure to F1 generation male mice. Mice were divided into 2 groups; (1) CON, control animals and (2) NP-50 ($50{\mu}g/L$), animals were treated with NP via drinking water. NP exposures were continuously conducted from parental pre-mating period until the postnatal day (PND) 55 of F1 offsprings. Mice were sacrificed on PND 55 and the tissue weights were measured. The initial body weights (at PND 21) and terminal body weights (PND 55) of the NP-50 animals were significantly lower than those of control animals (p<0.05). NP exposure induced a significant increase in the absolute weight of the testes (p<0.05). Conversely, the NP exposure caused significant decrease in the absolute weights of the epididymis (p<0.01), prostate (p<0.05) and seminal vesicle (p<0.05). Histopathological studies revealed that NP-treated animals exerted decreased seminiferous tubule diameters, reduced luminal area, and lower number of germ cells. Also some sloughing morphologies in the tubules were observed. In the caudal epididymis, fewer mature sperms and swollen epithelial cells were found in the NP-treated group. Our results confirmed that the subchronic low-dose NP exposure altered some male parameters and induced histopathological abnormalities in testis and epididymis of F1 mice. Since the NP dose used in this study is close to the average human daily NP exposure, our results could provide practically meaningful understanding of adverse effect of EDC in human.

• 분석과학
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• 제17권5호
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• pp.423-433
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• 2004

본 연구는 서울시 상수도 계통에서 내분비계장애물질인 BPA 및 NP의 실태조사를 위해 2002년 9월부터 2003년 12월까지 수행되었다. 상수원 유역의 한강 및 지류천중의 BPA 및 NP의 수준은 다음과 같았다: 팔당댐, $ND{\sim}0.033{\mu}g/L$ 및 $ND{\sim}0.823{\mu}g/L$ ; 경안천, $ND{\sim}0.659{\mu}g/L$ 및 N$D{\sim}3.827{\mu}g/L$ ; 왕숙천, $ND{\sim}0.528{\mu}g/L$ 및 $ND{\sim}12.724{\mu}g/L$. 서울시 6개 취수장 원수중의 BPA 수준은 $ND{\sim}0.260{\mu}g/L$, NP의 농도는 $0.122{\sim}2.724{\mu}g/L$의 범위에 있었다. 그리고 왕숙천 수역을 중심으로 한 3개 정수장의 여과수와 그 수돗물에서 BPA는 모두 불검출된 반면에, NP는 전지점에서 ppt 수준으로 검출되었다. 또한, 먹는물의 정수처리공정에서 NP의 제거율은 정수장별 차이가 없이 80{\sim}95%로 높게 나타났다. 따라서 본 연구결과는 상수도 계통에서 BPA 및 NP의 농도는 한강유역에 존재하는 점오염원 및 하수처리장 방류량에 의존된다는 것을 시사하고 있다.

• 방사성폐기물학회지
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• 제5권2호
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• pp.133-143
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• 2007

본 연구에서는 다성분의 모의 HLW 용액으로부터 $(TBP-TOA)/NDD-HNO_3$ 혼합추출 계에 의한 Tc, Np, U의 공분리 및 이의 순차 분리 적용 가능성을 평가하였다. 우선 제 3상 방지 및 TBP, TOA,질산 농도 등이 Tc, Np, U의 공분리에 미치는 영향 등을 고려하여 최적 조건으로 (30% TBP-0.5% TOA)/NDD-1M $HNO_3$ 계를 선정하였다. 이때 추출율은 Tc (81%), Np (85%), U (93%), Am/RE 원소 (9% 이하) Pd (약 8%), 기타 원소 (5% 이하)로 Tc, Np 및 U의 공분리는 매우 우수하였으나, 조업 측면에서 Zr의 선 제거 (약 99 % 이상)가 요구되었다. 그리고 공추출된 Tc, Np 및 U을 Tc (역추출제 : 5 M $HNO_3$)${\rightarrow}Np$ 환원 (역추출제 : 0.1 M AHA)${\rightarrow}U$ (역추출제 : 0.01 M $HNO_3$)의 순으로 순차 분리하여 각각의 분리계수를 평가하였으며, 이때 Tc은 95%, Np은 98%, U은 99%를 회수할 수 있었다.

• Nuclear Engineering and Technology
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• 제45권3호
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• pp.415-420
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• 2013

A determination method for $^{237}Np$ in spent nuclear fuel samples was developed using an isotope dilution method with $^{239}Np$ as a spike. In this method, inductively coupled plasma mass spectrometry (ICP-MS) was taken for the $^{237}Np$ instead of the previously used alpha spectrometry. $^{237}Np$ and $^{239}Np$ were measured by ICP-MS and gamma spectrometry, respectively. The recovery yield of $^{237}Np$ in synthetic samples was $95.9{\pm}9.7$% (1S, n=4). The $^{237}Np$ contents in the spent fuel samples were 0.15, 0.25, and $1.06{\mu}g/mgU$ and these values were compared with those from ORIGEN-2 code. A fairly good agreement between the measurements (m) and calculations (c) was obtained, giving ratios (m/c) of 0.93, 1.12 and 1.25 for the three PWR spent fuel samples with burnups of 16.7, 19.0, and 55.9 GWd/MtU, respectively.

• 공업화학
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• 제7권1호
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• pp.18-25
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• 1996

교반소요동력은 장치의 설계상 중요할 뿐만 아니라 교반 프로세스를 평가, 검토하는 데 매우 중요하다. 특히 실험실 레벨에서 플랜트로의 스케일업은 주로 동력기준으로 행해지기 때문에 장치형상의 선택과 동력의 설정이 교반효과를 결정한다. 따라서 본 연구에서는 난류역 완전방해판 조건하에서 경사 임펠러 및 2단 패들 임펠러의 적절한 동력상관식을 제시한다. $$Np_{(pitch)}=({\theta}/90^{\circ})Np_{(90^{\circ})}$$ 또한 임펠러 사이의 거리에 따른 동력수의 추산식을 제시한다. $$Np=7.09(n_p)^{0.7}(\frac{b_(double)}{d})(\frac{H}{D})^{0.18}$$, $${n_p}^{0.7}(\frac{b_{(double)}}d)$$<2 $$Np=8.73\{(n_p)^{0.7}(\frac{b_{(double)}}{d})\}^{0.7}(\frac{H}{D})^{0.18}$$, $${n_p}^{0.7}(\frac{b_{(double)}}d)$$>2.