• Food Science and Biotechnology
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• 제18권5호
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• pp.1237-1242
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• 2009

Polymethoxylated flavones (PMFs) extracted from Citrus sinensis 'Jincheng' peel were characterized by chromatographic and spectroscopic techniques. Seven individual PMF were identified. 3, 3', 4', 5, 6, 7-hexamethoxyflavone (HEX), nobiletin (NOB), heptamethoxyflavone (HEP), 5-demethylnobiletin (DN), and tangeretin (TAN) were characterized through electrospray ionization mass spectrometry (ESI-MS) in positive mode of protonated molecular ions $[M+H]^+$, the diagnostic fragment ions, together with the UV-Vis spectra and high performance liquid chromatography (HPLC) elution order from literature data. Sinensetin (SIN) and tetramethyl-O-scutellarein (SCU) were isolated and identified through their MS, $^1H$ nuclear magnetic resonance (NMR) and UV-Vis spectral studies. The levels of PMFs in peels from different cultivars of citrus fruits grown in China were determined for the first time. The results showed that C. aurantium 'Bitter orange' peel was the most promising variety for HEP. C. sinensis peel was a good source for SIN and SCU.

• Journal of Microbiology and Biotechnology
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• 제21권3호
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• pp.293-298
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• 2011

Nitrifying bacterial community structures of suspended and attached biomasses in a full-scale integrated fixed-film activated sludge process were investigated by analyzing 16S rRNA gene sequences obtained from pyrosequencing. The suspended biomass had a higher number of ammonia-oxidizing bacterial sequences (0.8% of total sequences) than the attached biomass (0.07%), although most of the sequences were within the Nitrosomonas oligotropha lineage in both biomasses. Nitrospira-like nitrite-oxidizing bacterial sequences were retrieved in the suspended biomass (0.06%), not in the attached biomass, whereas the existence of Nitrobacter-like sequences was not evident. The suspended biomass had higher nitrification activity (1.13 mg N/TSS/h) than the attached biomass (0.07 mg N/TSS/h). Overall, the results made it possible to conclude the importance of the suspended biomass, rather than the attached biomass, in nitrification in the wastewater treatment process studied.

• 대한기계학회논문집B
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• 제27권2호
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• pp.215-226
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• 2003

Experimental and numerical studies have been done to examine the effects of excess air ratio and tertiary air swirl number on the formation characteristics of NOx in a pilot scale combustor adopting a multi-air staged burner. In numerical calculation the mathematical models for turbulence, radiation and nitric oxide chemistry were taken into account. The radiative transfer equation was solved using the discrete ordinates method with the weighted sum of gray gases model. In the NOx chemistry model, the chemical reaction rates for thermal and prompt NOx were statistically averaged using a probability density function. The results were validated by comparison with measurements. For the experiment, a 0.2 MW pilot multi-staged air burner has been designed and fabricated. Using the numerical simulation developed here, a variation of thermal and prompt NOx formation was predicted by changing the excess air ratio and tertiary air swirl number. As the excess air ratio increased up to 1.9, the formation of the total as well as thermal NOx at exit increased while the prompt NOx decreased. The formation of thermal NOx was more affected by concentration of $O_2$ and $N_2$ than gas temperature. When the tertiary air swirl number increased, the formation of the total as well as the prompt NOx slightly decreased.

• 한국대기환경학회지
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• 제14권4호
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• pp.343-360
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• 1998

In this study, comprehensive air quality monitoring was undertaken with a variety of objectives to collect data on the levels of various indoor and ambient air quality parameters in the urban areas of Seoul and Taegu. The sampling sites were comprised of six offices, six residences and six restaurants in each city. The ambient air adjacent to the indoor sites was also simultaneously sampled for the same constituents. The sampling was conducted in two phases: summer of 1994 and winter of 1994/95. A range of air quality parameters were measured simultaneously, which include RSP, CO, COB, NOB, a range of VOC, airborne microbials, temperature, and relative humidity. The indoor and ambient levels of the pollutants measured in this study varied widely between the three types of environments studied. Comparison of median values for the three groups revealed that restaurants had higher indoor levels of most pollutants than homes or offices. There was also a clear pattern of the indoor levels of target pollutants being higher than those outdoors, particularly in restaurants. Concentrations of CO and most of the VOC were found to be significantly higher in the commercial districts, indicating the influence of vehicle exhaust emissions. A very wide range of VOC levels was documented in this study. Although median indoorloutdoor ratios indicated a generally increasing level of VOC indoors when compared to those outdoors, no statistically significant differences were found between indoor and outdoor VOC levels in homes and offices, implying the importance of ambient air quality in determining the quality of indoor air for homes and offices in urban areas. In addition, there was a general pattern of increasing concentrations from summer to winter, and similarly from outdoor to indoor air for nearly all target compounds. The seasonal differences in median levels were very clearly seen for fuel combustion related pollutants such as RSP, CO and VOC, this being attributed to the effects of increased fuel consumption during the cold season and to meteorological factors.

• International journal of advanced smart convergence
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• 제4권2호
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• pp.109-119
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• 2015

The interaction between wireless sensors such as Internet of Things (IoT) and Cloud is a new paradigm of communication virtualization to overcome resource and efficiency restriction. Cloud computing provides unlimited platform, resources, services and also covers almost every area of computing. On the other hand, Wireless Sensor Networks (WSN) has gained attention for their potential supports and attractive solutions such as IoT, environment monitoring, healthcare, military, critical infrastructure monitoring, home and industrial automation, transportation, business, etc. Besides, our virtual groups and social networks are in main role of information sharing. However, this sensor network lacks resource, storage capacity and computational power along with extensibility, fault-tolerance, reliability and openness. These data are not available to community groups or cloud environment for general purpose research or utilization yet. If we reduce the gap between real and virtual world by adding this WSN driven data to cloud environment and virtual communities, then it can gain a remarkable attention from all over, along with giving us the benefit in various sectors. We have proposed a Pub/Sub-based sensor virtualization framework Cloud environment. This integration provides resource, service, and storage with sensor driven data to the community. We have virtualized physical sensors as virtual sensors on cloud computing, while this middleware and virtual sensors are provisioned automatically to end users whenever they required. Our architecture provides service to end users without being concerned about its implementation details. Furthermore, we have proposed an efficient content-based event matching algorithm to analyze subscriptions and to publish proper contents in a cost-effective manner. We have evaluated our algorithm which shows better performance while comparing to that of previously proposed algorithms.

• Geosystem Engineering
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• 제21권5호
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• pp.243-250
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• 2018

This study investigated nitrite ($NO_2{^-}$) accumulation due to FA (Free Ammonia: $NH_3$) inhibition in an anaerobic-aerobic-anoxic (AOA) process reactor to mainly treat wastewater containing 302-610 mg/L of $NH_3/NH_4{^+}-N$. Based on an experimental operation focusing on the nitrification, it was observed that $NO_2{^-}$ was accumulated in the aerobic nitrification zone as pH increased, due to inhibition of $NO_2{^-}$ conversion to $NO_3{^-}$ by FA. This result implied FA inhibition to NOB ($NO_2{^-}$-Oxidizing Bacteria) for converting $NO_2{^-}$ to $NO_3{^-}$. The objective of this study is to develop a feasible monitoring procedure for early detection of the FA inhibition toward $NO_2{^-}$ accumulation and poor nitrification. Thus, in order to rapidly assess FA concentrations, an $NH_3$ probe was utilized to measure $NH_3$ concentrations together with applying a simple model prediction using the measured $NH_4{^+}$ concentrations, the Henry's law constant of $NH_3$ and measured pH. The predictive model $NH_3$ levels were verified by a good correlation (89%) with the corresponding measured data, but the model prediction underestimated FA concentrations at less than 7.4 and a little overestimated at pH above 7.5. Interestingly, accumulated $NO_2{^-}$ levels were roughly correlated with FA levels that were observed at delayed time points. This reflects the detected FA levels can be good indicators of $NO_2{^-}$ levels with some delayed time. $NO_2{^-}$ accumulation started at measured FA concentrations of higher than approximately 3 mg/L and ceased below that FA level.

• 공업화학
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• 제22권4호
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• pp.411-415
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• 2011

본 연구에서는 화학적 구조가 다른 thiuram계 tetramethyl thiuram disulfide (TMTD), dipenta methylene thiuram tertasulfied (DPTT), thiazole계 2-mercapto benzothiazole (MBT), 2,2'-dithiobisbenzothiazole (MBTS), sulfenamide계 n-cyclohexyl benzothiazyl-2-sulfenamide (CBS), n-oxydiethylene benzo-thiazyl-2-sulfenamide (NOBS)를 사용하여 각각의 촉진제가 실리카가 충진된 천연고무의 가황 특성 및 기계적 물성에 미치는 영향을 비교 평가하였다. TMTD는 상대적으로 빠른 가류 속도와 높은 최대 토크값($T_{max}$), 우수한 기계적 물성을 보였고 MBT, MBTS는 상대적으로 중간 정도의 가황 시간과 $T_{max}$, 기계적 물성을 보였다. 마지막으로 NOBS는 느린 가황 시간과 낮은 기계적 물성을 나타낸 반면 중간 값의 $T_{max}$를 나타냈다.

• Journal of Veterinary Science
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• 제23권1호
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• pp.4.1-4.15
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• 2022

Background: Flavonoids are natural polyphenols found widely in citrus fruit and peel that possess anti-adipogenic effects. On the other hand, the detailed mechanisms for the antiadipogenic effects of flavonoids are unclear. Objectives: The present study observed the anti-adipogenic effects of five major citrus flavonoids, including hesperidin (HES), narirutin (NAR), nobiletin (NOB), sinensetin (SIN), and tangeretin (TAN), on AMP-activated protein kinase (AMPK) activation in palmitate (PA)-treated HepG2 cells. Methods: The intracellular lipid accumulation and triglyceride (TG) contents were quantified by Oil-red O staining and TG assay, respectively. The glucose uptake was assessed using 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG) assay. The levels of AMPK, acetyl-CoA carboxylase (ACC), and glycogen synthase kinase 3 beta (GSK3β) phosphorylation, and levels of sterol regulatory element-binding protein 2 (SREBP-2) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) expression were analyzed by Western blot analysis. The potential interaction between the flavonoids and the γ-subunit of AMPK was investigated by molecular docking analysis. Results: The flavonoid treatment reduced both intracellular lipid accumulation and TG content in PA-treated HepG2 cells significantly. In addition, the flavonoids showed increased 2-NBDG uptake in an insulin-independent manner in PA-treated HepG2 cells. The flavonoids increased the AMPK, ACC, and GSK3β phosphorylation levels and decreased the SREBP-2 and HMGCR expression levels in PA-treated HepG2 cells. Molecular docking analysis showed that the flavonoids bind to the CBS domains in the regulatory γ-subunit of AMPK with high binding affinities and could serve as potential AMPK activators. Conclusion: The overall results suggest that the anti-adipogenic effect of flavonoids on PA-treated HepG2 cells results from the activation of AMPK by flavonoids.

• 공업화학
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• 제34권5호
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• pp.541-547
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• 2023

The effect of temperature and influent alkalinity/ammonia (K/A) ratio on the start-up of the partial nitrification (PN) process for an activated sludge-based domestic wastewater treatment was studied. Two different sequence batch reactors (SBR) were operated at 26 ℃ and 32 ℃. The relationship between temperature and the concentration of free ammonia (FA) and free acid nitrite (FNA) was investigated. A stable PN process was achieved in the 32 ℃ reactor when the influent ammonium concentration was lower than 150 mg-N/L. In contrast, the PN process in the 26 ℃ reactor had a higher nitrite accumulation rate (NAR) and ammonium removal efficiency (ARE) when the influent ammonia concentration was increased to more than 150 mg-N/L. Then three different ranges of the K/A ratio were applied to an SBR reactor. In the K/A range of 2.48~1.65, the SBR reactor achieved the highest NAR ratio (75.78%). This ratio helps to achieve the appropriate level of alkalinity to maintain a stable pH and provide a sufficient amount of inorganic carbon source for the activity of microorganisms. At the same time, FA and FNA values also reached the threshold to inhibit nitrite-oxidizing bacteria (NOB) without a significant effect on ammonia-oxidizing bacteria (AOB). Results showed that the control of temperature and K/A ratio during the start-up period may be important in establishing a stable and steady PN process for the treatment of domestic wastewater.

• 한국산림과학회지
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• 제80권2호
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• pp.220-231
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• 1991

본(本) 연구(硏究)는 침 활엽수에서 몇 수종(樹種)을 선정하여 독일(獨逸)의 표준화(標準化)된 작업방법(作業方法)과 우리나라 임업생산(林業生產)에서 실제(實際) 이용(利用)되고 있는 작업방법(作業方法)을 비교(比較) 연구(硏究)하여 우리의 실정에 맞는 표준화(標準化)된 작업방법(作業方法)을 확실하게 구명(究明)하여 임업경영(林業經營)에서의 생산비용(生產費用)(인건비(人件費)) 절감(節減)과 아울러 산림작업(山林作業)의 성력화(省力化)에 효율적(效率的)으로 적용(適用)하고자 한다. 1. 두가지의 작업방법(作業方法)을 비교(比較)한 결과(結果) 동일한 작업조건(作業條件)에서 순수작업(純粹作業) 소요시간(所要時間)은 별 차이(差異)가 없었다. 2. 수종별(樹種別) 순수작업시간(純粹作業時間)을 요소작업별(要素作業別)로 분석(分析)한 벌도(伐倒)와 절동시간(切棟時間)은 침엽수종(針葉樹種)보다 참나무가 더 많은 시간소요(時間所要)를 보여 주는 바, 이는 재질이 단단함 때문인 것으로 판단되며, 조재시간(造材時間)은 참나무보다 침엽수종(針葉樹種)이 시간소요(時間所要)가 더 많은 것은 침엽수종(針葉樹種)이 참나무에 비하여 가지수(NOB)가 더 많기 때문인 것으로 나타났다. 3. 종합적(綜合的)으로 볼 때 작업시간(作業時間)은 보통 흉고직경(胸高直徑) 1cm가 증가하면서 작업방법(作業方法)에 따라 약간의 차이는 있으나 11-13초가 증가(增加)되었다. 4. 이 결과(結果)로부터 각기 다른 두 작업방법(作業方法)에서 작업(作業)에 소요(所要)되는 시간(時間) 차이(差異)는 거의 없어 우리나라에서도 독일(獨逸)에서 이용되고 있는 1인1조 작업방법(作業方法)으로 전환(轉換)할 것을 추천(推薦)하며, 앞으로 인건비(人件費)의 상승(上昇)과 산촌(山村)의 인력난(人力難)으로 인하여 점차 어려워지는 실정에 있어 현 시점에서 작업원(作業員)의 기능교육(技能敎育)을 확대실시하고 동시에 이와 같은 표준적(標準的)인 작업방법(作業方法)을 적용(適用)하여 생산성(生產性) 향상(向上)을 도모(圖謀)해야 한다.