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http://dx.doi.org/10.4014/jmb.1009.09042

Nitrifying Bacterial Community Structure of a Full-Scale Integrated Fixed-Film Activated Sludge Process as Investigated by Pyrosequencing  

Kim, Taek-Seung (School of Civil, Environmental and Architectural Engineering, Korea University)
Kim, Han-Shin (School of Civil, Environmental and Architectural Engineering, Korea University)
Kwon, Soon-Dong (School of Civil, Environmental and Architectural Engineering, Korea University)
Park, Hee-Deung (School of Civil, Environmental and Architectural Engineering, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.3, 2011 , pp. 293-298 More about this Journal
Abstract
Nitrifying bacterial community structures of suspended and attached biomasses in a full-scale integrated fixed-film activated sludge process were investigated by analyzing 16S rRNA gene sequences obtained from pyrosequencing. The suspended biomass had a higher number of ammonia-oxidizing bacterial sequences (0.8% of total sequences) than the attached biomass (0.07%), although most of the sequences were within the Nitrosomonas oligotropha lineage in both biomasses. Nitrospira-like nitrite-oxidizing bacterial sequences were retrieved in the suspended biomass (0.06%), not in the attached biomass, whereas the existence of Nitrobacter-like sequences was not evident. The suspended biomass had higher nitrification activity (1.13 mg N/TSS/h) than the attached biomass (0.07 mg N/TSS/h). Overall, the results made it possible to conclude the importance of the suspended biomass, rather than the attached biomass, in nitrification in the wastewater treatment process studied.
Keywords
IFAS; pyrosequencing; AOB; NOB; activated sludge;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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