• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.4
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• pp.329-338
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• 1985

This study was undertaken in order to research formation of nitrosamine and its related compounds by salt concentration and nitrate content during Korean native soysauce fermentation. The results from measuring the changes of nitrite and dimethylamine content can be summerized as follows. As the nitrate content in used water was getting higher and the salt concentration was getting lower, the soysauce fermentation was abnormal. As the salt concentration was getting higher, the reduction of nitrate and formation of nitrite were delayed. But whether the nitrate content in used water was higher or not, the nitrite was continuously remained. An addition of ascorbic acid restrained the reduction of nitrate, and simultaneously, it could eliminate the nitrite effectively. As the nitrate content in used water, was getting higher, the content of dimethylamine was getting lower. Nitrosodimethylamine was detected from 0 to 261.34 ppb.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.4 s.59
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• pp.219-225
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• 2006

In this study, we evaluated anti-oxidation, whitening and anti-inflammatory effects of Elsholtzia ciliata extract for use as the cosmeceuticals. Elsholtzia ciliata extracts (30, 70 and 100% methanol extract) exhibited a significant tree radical scavenging effect (up to 80% over 0.025% concentration of 30 and 70% methanol extract, over 0.01% concentration of 100% methanol extract) against DPPH radical generation and showed a significant inhibitory effect (up to 80% over 0.1% concentration) on melanin synthesis in B-16 Melanoma cells. We separated 4 fractions from Elsholtzia ciliata extract (70% methanol extract) by MPLC. The 1st, 2nd, and 3rd fractions showed anti-oxidation (DPPH radical scavenging activity and suppressive effect on Mn-SOD), whitening(inhibitory effect on melanin synthesis) and anti-inflammatory (suppressive effects on $IL-1{\alpha}$, IL-6, COX-2, and total NO synthesis) effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.8
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• pp.1097-1101
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• 2010

The aim of the present study is to investigate the anti-inflammatory effect of a vegetable soup (VS). The present study was designed to determine the effect of the vegetable soup on pro-inflammatory factors such as NO, iNOS and TNF-$\alpha$ in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The cell toxicity was determined by MTS assay. To evaluate the anti-inflammatory effect of vegetable soup, amount of NO was measured using the NO detection kit and the iNOS expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). Also, proinflammatory cytokines were measured by ELISA kit. The results showed that the vegetable soup reduced NO, iNOS and TNF-$\alpha$ production without cytotoxicity. Our results suggest that the vegetable soup may have an anti-inflammatory property through suppressing inflammatory mediator productions and appears to be useful to develop the functional food realted to anti-inflammation.

• The Korean Journal of Mycology
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• v.40 no.1
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• pp.69-71
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• 2012

During search for novel bioactive materials from natural resources with the potential as health food and alternative medicine, the culture broth of Cordyceps longissima (CL) J106, J144 and C. scarabaeicola (CS) J94, J123 were prepared, and their effect on cytotoxicity and nitric oxide (NO) production in RAW 264.7 cells were investigated. Whereas the culture broth of CL J144 and CS J123 had cytotoxicity in RAW 264.7 cells, that of CL J106 and CS J94 did not. The culture broth of CL J106 and CS J94 suppressed NO production in RAW 264.7 cells activated with lipopolysaccharide (LPS) at a dose-dependent manner. These results suggest that culture broth, a by-product of Cordyceps, may have active compounds with anti-inflammatory effect. In addition, it appears that their biological activity is dependent on the strains in spite of the same species.

• Journal of Nutrition and Health
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• v.50 no.1
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• pp.25-31
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• 2017

Purpose: Neuroinflammation is mediated by activation of microglia implicated in the pathogenesis of neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Inhibition of neuroinflammation may be an effective solution to treat these brain disorders. Petalonia binghamiae is known as a traditional food, based on multiple biological activities such as anti-oxidant and anti-obesity. In present study, the anti-neuroinflammatory potential of Petalonia binghamiae was investigated in LPS-stimulated BV2 microglial cells. Methods: Cell viability was measured by MTT assay. Production of nitric oxide (NO) was examined using Griess reagent. Expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was detected by Western blot analysis. Activation of nuclear factor ${\kappa}B$ ($NF-{\kappa}B$) signaling was examined by nuclear translocation of $NF-{\kappa}B$ p65 subunit and phosphorylation of $I{\kappa}B$. Results: Extract of Petalonia binghamiae significantly inhibited LPS-stimulated NO production and iNOS/COX-2 protein expression in a dose-dependent manner without cytotoxicity. Pretreatment with Petalonia binghamiae suppressed LPS-induced $NF-{\kappa}B$ p65 nuclear translocation and phosphorylation of $I{\kappa}B$. Co-treatment with Petalonia binghamiae and pyrrolidine duthiocarbamate (PDTC), an $NF-{\kappa}B$ inhibitor, reduced LPS-stimulated NO release compared to that in PB-treated or PDTC-treated cells. Conclusion: The present results indicate that extract of Petalonia binghamiae exerts anti-neuroinflammation activities, partly through inhibition of $NF-{\kappa}B$ signaling. These findings suggest that Petalonia binghamiae might have therapeutic potential in relation to neuroinflammation and neurodegenerative diseases.

• Journal of dental hygiene science
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• v.11 no.5
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• pp.411-416
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• 2011

Streptococcus mutans (S. mutans) is the major causative bacteria in dental caries. Xylitol is effective anticarious natural sugar substitute by inhibiting the virulence of S. mutans. However, long-term xylitol consumption leads to the emergence of the xylitol-resistant (XR) strains which means xylitol is no more inhibited their growth. We therefore confirmed the general characteristics and the virulence factors of the xylitol-sensitive (XS) and XR S. mutans for different concentrations of xylitol. S. mutans KCTC 3065 was maintained in TYE medium containing 0.4% glucose with 1% xylitol during 30 days at $37^{\circ}C$, 10% $CO_2$ to form XR strain. The strains were transferred to new medium every 24 hr and the same procedures without xylitol were repeated for the formation of XS S. mutans. Both XS and XR were cultured in different concentrations of xylitol (0%, 0.1% and 1%) then, cell growth, acid production and mRNA expression of gtf genes were analyzed. Xylitol reduced the cell growth of XS S. mutans in dose-dependent manner, but not reduced that of XR. Xylitol inhibited acid production of XS in dose-dependent manner, but not inhibited that of XR. Xylitol reduced the gtfB and gtfD mRNA expression of XS S. mutans which genes synthesized soluble and insoluble extracellular polysaccharides, but not reduced that of XR. These results indicate that the virulence of XR S. mutans is different characters of XS strains, which suggests XR strains may have different cariogenicity of XS strains. Further study is needed to explain the mechanism related to extracellular polysaccharide in the XR strains.

• Journal of Applied Biological Chemistry
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• v.61 no.2
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• pp.141-150
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• 2018

In this study, the inflammation of ethanol extracts from Caryopteris incana (CI) and fermented C. incana (FCI) on induced to lipopolysaccharide with Raw 264.7 cell was tested. The composition profile of L. plantarum was changed by fermentation, and confirmed by HPLC analysis. We performed the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyltetrazolium bromide assay to evaluate the toxicity of CI and FCI extracts. In cell viability, cell toxicity was not shown at 5, 10 and $15{\mu}g/mL$ of CI extracts and 10, 20, 30 and $40{\mu}g/mL$ of FCI extracts. The results of inducible nitric oxide synthase and cyclooxygenase-2 protein production were confirmed to be inhibitory in a concentration-dependent manner, respectively. Additionally, protein expression of nitric oxide and prostaglandin $E_2$ by CI and FCI extracts were also inhibited in a concentration-dependent manner. In the result of pro-inflammatory cytokine, $15{\mu}g/mL$ concentration of CI extracts was showed tumar necrosis factor $(TNF)-{\alpha}$ (57.3%), interleukin (IL)-6 (35.2%), and $IL-1{\beta}$ (48.0%), respectively. And $40{\mu}g/mL$ of FCI extracts was showed $TNF-{\alpha}$ (34.6%), IL-6 (32.1%), and $IL-1{\beta}$ (30.0%), respectively. These results suggest that FCI extracts showed better effect of anti-inflammatory than CI extracts. Therefore, it was found that both CI and FCI can be used as an excellent material for the development of new anti-inflammatory resource.

• Journal of Life Science
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• v.26 no.12
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• pp.1422-1430
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• 2016

This study was performed to investigate the modulatory effects of two prototypes of Panax ginseng saponin fractions, 20(S)-protopanaxadiol saponins (PDS) and 20(S)-protopanaxatriol saponins (PTS), on the induction of inflammatory mediators in lipopolysaccharide (LPS)-treated RAW264.7 murine macrophage cells. For this purpose, RAW264.7 cells were treated with LPS ($10{\mu}g/ml$) before, after, or simultaneously with PDS or PTS ($150{\mu}g/ml$), and the released level of nitric oxide (NO) and expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated. When RAW264.7 cells were treated with LPS and ginseng saponin fractions simultaneously for 24 hr, PTS, compared to PDS, more strongly attenuated the NO production induced by LPS treatment. When the cells were pretreated with LPS for 2 hr followed by PDS or PTS treatment for 24 hr, both ginseng saponins strongly reduced NO release. The pretreatment of RAW264.7 cells with PDS or PTS for 2 hr followed by LPS treatment for 24 hr significantly attenuated the LPS-induced production of NO. PTS showed stronger inhibitory potency to NO generation than PDS. Our western blot experiment showed that both PDS and PTS ($150{\mu}g/ml$) also significantly down-regulated the expressions of iNOS and COX-2 induced by LPS treatment. Our results suggest that both PDS and PTS possess strong protective effects against LPS-stimulated inflammation and that their protective effects are mediated by the suppression of NO synthesis via down-regulation of pro-inflammatory enzymes, iNOS, and COX-2 in the RAW264.7 cells.

• KSBB Journal
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• v.11 no.3
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• pp.374-379
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• 1996

The production of extracellular mannitot by an efficient mannitol-producing bacterium, Lactobacillus sp. KY-107 was studied in shake flask culture using the modified MRS medium. Maximum mannitol production was obtained with fructose as the sole carbon source. Within 95 hours of incubation, a final concentration of 70g/L of mannitol from 100g/L fructose was obtained with an indicated yield of 86% based on fructose consumed. However, higher concentrations of fructose could not effectively be transformed to mannitol due to a lack of osmotolerance. The strain produced no other polyols such as glycerol and sorbitol as by-products. Yeast extract was best nitrogen source and high levels of inorganic phosphate up to 10g/L did not show any detrimental effect for mannitol formation. Manganese ion played important role in both cell growth and mannitol production. The optimum culture temperature and initial pH were $35^{\circ}C$ and 6-8, respectively.

• Journal of Life Science
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• v.28 no.9
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• pp.1022-1029
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• 2018

This study was carried out to search for the anti-inflammatory activities of ethanol extracts obtained from 5 kinds of oriental medical plant; Pleuropterus multiflorus extract (PME), Acorus calamus L. extract (ACE), Lithospermum erythrorhizon Siebold & Zucc. extract (LEE), Xanthium strumarium L. extract (XSE), Lonicera japonica extract (LJE), which have traditionally been used as a drug in oriental medical plants in Korea. XSE showed cytotoxicity at 100, $200{\mu}g/ml$ concentration in RAW264.7 cells (p<0.05) and ACE showed cytotoxicity at $200{\mu}g/ml$ concentration in RAW264.7 cells (p<0.05). But other oriental medical plants did not showed cytotoxicity was observed in RAW264.7 cells below $200{\mu}g/ml$ concentration. These extracts at non-toxic concentrations showed anti-inflammatory effects. PME, ACE, XSE and LJE showed a concentration-dependent inhibitory effect on NO production and $PGE_2$ production in LPS-induced RAW264.7 cells. In particular, XSE showed the highest NO production inhibition ($52.9{\mu}g/ml$, $IC_{50}$) as well as the highest $PGE_2$ production inhibition at $50{\mu}g/ml$ (73.6%). ACE and LEE showed cell proliferation effects on HaCaT keratinocyte cells. Especially, LEE showed 21.1, 53.5 and 99.6% proliferative activity by incubation for 1, 3, 5days at $100{\mu}g/ml$ concentration. ACE also showed 11.2, 26.0% proliferative activity for 1day and 3days at $10{\mu}g/ml$ concentration. As a result of this study, ethanol extracts obtained from 5 kinds of oriental medical plant showed anti-inflammatory activity and HaCaT cell regeneration effect.