• YAKHAK HOEJI
• /
• v.50 no.2
• /
• pp.93-98
• /
• 2006

Human foamy virus (HFV) integrase mediates integration of viral c-DNA into cellular DNA. In this process, HFV prointegration complex (PIC) in which integrase is a key component moves to nuclei of the infected cells and leads to integration of viral DNA to the cellular genome, which is essential in viral life cycle. In general nuclear localization signals (NLS) have been suggested to be involved in localizing retroviral PIC to nuclei, but the mechanisms for nuclear localization of the HFV PIC remains unclear. To functionally identify the NLS of HFV integrase, various subdomains of the protein were expressed as GFP fusions and their subcellular locations were analyzed with confocal laser scanning microscopy. Wild type HFV integrase was karyophilic by targeting the fusion protein to nuclei of the COS-1 and 293T cells. Our results showed that strong NLS of HFV integrase was mapped to the C-terminal regions. In addition the karyophilic properties of N-terminal and central regions are not individually strong enough to direct localization of the fusion proteins to nuclei, but their cooperative activity for nuclear import was confirmed.

• Molecules and Cells
• /
• v.27 no.3
• /
• pp.359-363
• /
• 2009

Chfr, a checkpoint with FHA and RING finger domains, plays an important role in cell cycle progression and tumor suppression. Chfr possesses the E3 ubiquitin ligase activity and stimulates the formation of polyubiquitin chains by Ub-conjugating enzymes, and induces the proteasome-dependent degradation of a number of cellular proteins, including Plk1 and Aurora A. While Chfr is a nuclear protein that functions within the cell nucleus, how Chfr is localized in the nucleus has not been clearly demonstrated. Here, we show that nuclear localization of Chfr is mediated by nuclear localization signal (NLS) sequences. To reveal the signal sequences responsible for nuclear localization, a short lysine-rich stretch (KKK) at amino acid residues 257-259 was replaced with alanine, which completely abolished nuclear localization. Moreover, we show that nuclear localization of Chfr is essential for its checkpoint function but not for its stability. Thus, our results suggest that NLS-mediated nuclear localization of Chfr leads to its accumulation within the nucleus, which may be important in the regulation of Chfr activation and Chfr-mediated cellular processes, including cell cycle progression and tumor suppression.

• Journal of the Korea Institute of Military Science and Technology
• /
• v.14 no.4
• /
• pp.590-597
• /
• 2011

The IDRS provides detection, classification and bearing/range estimation by performing wavefront curvature analysis on an intercepted active transmission from target. Especially, a estimate of the target bearing/range that significantly affects the optimal operation of own submarine is required. Target bearing/range can be estimated by wavefront curvature ranging which use the difference of time arrival at sensors. But estimation ambiguity occur in bearing/range estimation due to a number of peaks caused by high center frequency and limited bandwidth of the intercepted active transmission and distortion caused by noise. As a result the bearing/range estimation performance is degraded. To estimate target bearing/range correctly, bearing/range estimation method that eliminate estimation ambiguity is required. In this paper, therefore, for wavefront curvature ranging, NLS cost function with curve fitting method is proposed, which provide robust bearing/range estimation performance by eliminating estimation ambiguity. Through simulation the performance of the proposed bearing/range estimation methods are verified.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.27 no.8A
• /
• pp.814-821
• /
• 2002

The SPIHT(set partitioning in hierarchical tree) is efficient and well-known in the zerotree coding algorithm. However SPIHT's high memory requirement is a major difficulty for hardware implementation. In this paper we propose low-memory and fast zerotree algorithm. We present following three methods for reduced memory and fst coding speed. First, wavelet transform by lifting has a low memory requirement and reduced complexity than traditional filter bank implementation. The second method is to divide the wavelet coefficients into a block. Finally, we use NLS algorithm proposed by Wheeler and Pearlman in our codec. Performance of NLS is nearly same as SPIHT and reveals low and fixed memory and fast coding speed.

• Nuclear Medicine and Molecular Imaging
• /
• v.41 no.4
• /
• pp.317-325
• /
• 2007

Purpose: Biological parameters can be quantified using dynamic PET data with compartment modeling and Nonlinear Least Square (NLS) estimation. However, the generation of parametric images using the NLS is not appropriate because of the initial value problem and excessive computation time. In irreversible model, Patlak graphical analysis (PGA) has been commonly used as an alternative to the NLS method. In PGA, however, the start time ($t^*$, time where linear phase starts) has to be determined. In this study, we suggest a new Multiple Linear Analysis for irreversible radiotracer (MLAIR) to estimate fluoride bone influx rate (Ki). Methods: $[^{18}F]Fluoride$ dynamic PET scans was acquired for 60 min in three normal mini-pigs. The plasma input curve was derived using blood sampling from the femoral artery. Tissue time-activity curves were measured by drawing region of interests (ROls) on the femur head, vertebra, and muscle. Parametric images of Ki were generated using MLAIR and PGA methods. Result: In ROI analysis, estimated Ki values using MLAIR and PGA method was slightly higher than those of NLS, but the results of MLAIR and PGA were equivalent. Patlak slopes (Ki) were changed with different $t^*$ in low uptake region. Compared with PGA, the quality of parametric image was considerably improved using new method. Conclusion: The results showed that the MLAIR was efficient and robust method for the generation of Ki parametric image from $[^{18}F]Fluoride$ PET. It will be also a good alternative to PGA for the radiotracers with irreversible three compartment model.

• Journal of Communications and Networks
• /
• v.5 no.4
• /
• pp.303-308
• /
• 2003

We present a statistical model for the path loss of ultrawideband (UWB) channels in indoor environments. In contrast to our previously reported measurements, the data reported here are for a bandwidth of 6GHz rather than 1.25GHz; they encompass commercial buildings in addition to single-family homes (20 of each); and local spatial averaging is included. As before, the center frequency is 5.0GHz. Separate models are given for commercial and residential environments and, within each category, for lineof sight (LOS) and non-line-of-sight (NLS) paths. All four models have the same mathematical structure, differing only in their numerical parameters. The two new models (LOS and NLS) for residences closely match those derived from the previous measurements, thus affirming the stability of our path loss modeling. We find, also, that the path loss statistics for the two categories of buildings are quite similar.

• Journal of the Korean Operations Research and Management Science Society
• /
• v.38 no.1
• /
• pp.45-59
• /
• 2013

A long-term forecasting method for a new product in early stage of diffusion is proposed. The method includes a constrained non-linear least square estimation with the logistic diffusion model. The constraints would be critical market informations such as market potential, peak point, and take-off. Findings on 20 cases having almost full life cycle are that (i) combining any market information improves the forecasting accuracy, (ii) market potential is the most stable information, and (iii) peak point and take-off information have negative effect in case of overestimation.

• Mycobiology
• /
• v.50 no.5
• /
• pp.259-268
• /
• 2022

The nuclear import of proteins is a fundamental process in the eukaryotes including plant. It has become evident that such basic process is exploited by nuclear effectors that contain nuclear localization signal (NLS) and are secreted into host cells by fungal pathogens of plants. However, only a handful of nuclear effectors have been known and characterized to date. Here, we first summarize the types of NLSs and prediction tools available, and then delineate examples of fungal nuclear effectors and their roles in pathogenesis. Based on the knowledge on NLSs and what has been gleaned from the known nuclear effectors, we point out the gaps in our understanding of fungal nuclear effectors that need to be filled in the future researches.

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2006.05a
• /
• pp.491-493
• /
• 2006

• Journal of Microbiology and Biotechnology
• /
• v.30 no.1
• /
• pp.109-117
• /
• 2020

Cre recombinase is widely used to manipulate DNA sequences for both in vitro and in vivo research. Attachment of a trans-activator of transcription (TAT) sequence to Cre allows TAT-Cre to penetrate the cell membrane, and the addition of a nuclear localization signal (NLS) helps the enzyme to translocate into the nucleus. Since the yield of recombinant TAT-Cre is limited by formation of inclusion bodies, we hypothesized that the positively charged arginine-rich TAT sequence causes the inclusion body formation, whereas its neutralization by the addition of a negatively charged sequence improves solubility of the protein. To prove this, we neutralized the positively charged TAT sequence by proximally attaching a negatively charged poly-glutamate (E12) sequence. We found that the E12 tag improved the solubility and yield of E12-TAT-NLS-Cre (E12-TAT-Cre) compared with those of TAT-NLS-Cre (TAT-Cre) when expressed in E. coli. Furthermore, the growth of cells expressing E12-TAT-Cre was increased compared with that of the cells expressing TAT-Cre. Efficacy of the purified TAT-Cre was confirmed by a recombination test on a floxed plasmid in a cell-free system and 293 FT cells. Taken together, our results suggest that attachment of the E12 sequence to TAT-Cre improves its solubility during expression in E. coli (possibly by neutralizing the ionic-charge effects of the TAT sequence) and consequently increases the yield. This method can be applied to the production of transducible proteins for research and therapeutic purposes.