• 한국패류학회지
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• 제22권2호
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• pp.109-113
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• 2006

연체동물 유래 아미노산 서열 22,138 개에서 NLS가 예측되는 아미노산 서열은 266 개였으며 이는 연체동물 전체 아미노산 중 1.2% 정도였다. 또한 현재 등재되어 있는 연체동물 8,314 종 중 NLS를 포함한 아미노산이 밝혀진 생물은 60여종에 불과 하였다. 현재 알려진 연체동물 서열 중에는 두족 강의 경우가 NLS를 포함한 아미노산이 많을 것으로 예측되었다.

• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.788-794
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• 2015

Cationic liposomes have been actively used as gene delivery vehicles despite their unsatisfactory efficiencies because of their relatively low toxicity. In this study, we designed novel heterodimeric peptides as nonviral gene delivery systems from TAT and NLS peptides using cysteine-to-cysteine disulfide bonds between the two. Mixing these heterodimeric peptides with DNA before mixing with lipofectamine resulted in higher transfection efficiencies in MCF-7 breast cancer cells than mixing unmodified TAT, NLS, and a simple mixture of TAT and NLS with DNA, but did not show an adverse effect on cell viability. In gel retardation assays, the DNA binding affinities of heterodimeric peptides were stronger than NLS but weaker than TAT. However, this enhancement was only observed when heterodimeric peptides were premixed with DNA before being mixed with lipofectamine. The described novel transfection-enhancing peptide system produced by the heterodimerization of TAT and NLS peptides followed by simple mixing with DNA, increased the gene transfer efficiency of cationic lipids without enhancing cytotoxicity.

• 대한산업공학회지
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• 제37권1호
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• pp.74-82
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• 2011

The Bass model is a cornerstone in diffusion theory which is used for forecasting demand of durables or new services. Three well-known estimation methods for parameters of the Bass model are Ordinary Least Square (OLS), Maximum Likelihood Estimator (MLE), Nonlinear Least Square (NLS). In this paper, a hybrid method incorporating OLS and NLS is presented and it's performance is analyzed and compared with OLS and NLS by using simulation data and empirical data. The results show that NLS has the best performance in terms of accuracy and our hybrid method has the best performance in terms of stability. Specifically, hybrid method has better performance with less data. This result means much in practical aspect because the avaliable data is little when a diffusion model is used for forecasting demand of a new product.

• 해양환경안전학회지
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• 제22권7호
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• pp.846-853
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• 2016

해상 유통되는 유해액체물질(NLS)의 유출사고대비 물질군 선정을 위해 596종의 NLS를 대상으로 위해성 DB를 구축하고 우선 순위 선정시스템을 통해 전체 우선순위를 선정하였다. 우선순위 목록을 바탕으로 2014-2015년 해상유통물질 158종을 추출한 뒤 물질군 구분 기준을 적용하여 0~3순위의 4개 물질군을 제시하였다. 국가차원의 NLS 유출사고대비를 위해서는 물동량 및 유해성이 높은 0~1순위 물질군의 집중관리와 함께 2~3순위 물질군의 정보 구축작업이 지속되어야 한다. 항만별로는 NLS 유통 유형이 다르므로 각 항만의 물질별 유통특성을 파악한 뒤 0~1순위 물질군 위주로 관리하는 것이 효율적일 것으로 판단된다. 또한, 동해남부권역(울산, 부산), 남해중부권역(광양, 여수), 서해중부권역(평택, 대산, 인천)을 NLS 사고대비를 위한 특별관리구역으로 지정하여 해상유통되는 NLS 의 감시 감독을 강화하고 방제 장비, 자재 및 약제를 집중 배치해야 할 것으로 판단된다. 향후, 위해성 DB의 구축과정에서 나타난 위해성 정보 부재 물질들의(만성독성) 지속적인 생산 및 보완이 필요하며, 특히 수생태 독성의 경우 해양생물종을 대상으로 한 자료 생산 및 확보가 지속되어야 할 것이다. 또한, 해상 HNS 사고 관리를 위해서는 HNS 해상유통에 대한 정보를 확인할 수 있는 시스템이 조속히 구축되어야 한다.

• 한국생활과학회지
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• 제16권4호
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• pp.761-773
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• 2007

The study undertakes an examination of nutrition labelling system and offers a strategic framework for improvement of the system in Korean context. Thus this study includes a review of Korean current nutrition labelling system (NLS), development of a strategy or a further study for its revision of NLS, and a suggestion of revised nutrition labelling guidelines. Participants were 600 university students in Busan and were asked to fill in a questionnaire. The data collected were processed with the SPSS statistical program to produce its frequency, percentage, average, and standard deviation with One-Way Anova and Duncan Test. The findings are as follows: the levels of consumer's practical use and awareness of NLS are low, the levels of their trust and satisfaction and their necessity for NLS are low too. Consequently, the consumer's attitudes to NLS are not related to nutrition labelling method(? system).

• 천문학회보
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• 제38권2호
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• pp.46.1-46.1
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• 2013

WIth high accretion rate and low black hole mass, narrow line seyfert 1 galaxies (NLS1s) are an interesting sub-class of AGNs. To investigate whether NLS1s follow the same M-${\sigma}*$ relation as other AGNs, we selected a sample of 110 NLS1s at relatively low redshift z < 0.1 from SDSS DR7 by constraining the FWHM of Ha broad component, and determined their black hole masses. We measured stellar velocity dispersion of 65 objects which showed strong enough stellar lines in the SDSS spectra, while we adopted the ${\sigma}*$ measurements of 45 objects from Xiao et al. 2011. We find that NLS1s follow the M-${\sigma}*$ relation of active and inactive galaxies while there is a dependency due to the galaxy inclination, which probably cause rotational broadening of stellar absorption lines.

• 천문학회보
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• 제37권1호
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• pp.47.1-47.1
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• 2012

Given high accretion rates close to the Eddington limit, narrow-line Seyfert 1 galaxies (NLS1) are arguably the most important AGN subclass in investigating the origin of the black hole mass-galaxy stellar velocity dispersion ($M_{BH}-{\sigma}$) relation. Currently, it is highly debated whether NLS1s are offset from the local $M_{BH}-{\sigma}$ relation. The controversy mainly comes from the fact that the [OIII] line width has been used as a proxy for stellar velocity dispersion due to the difficulty of measuring stellar velocity dispersion in NLS1s. Using the SDSS spectra of a sample of 105 NLS1, we performed multi-component fitting analysis to separate stellar absorption lines from strong AGN [FeII] complex in order to directly measure stellar velocity dispersion. We will present the result of decomposition analysis and discuss whether NLS1s follow the same $M_{BH}-{\sigma}$ relation based on the direct measurements of stellar velocity dispersion.

• 한국수정란이식학회지
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• 제19권1호
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• pp.27-34
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• 2004

This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.

• IMMUNE NETWORK
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• 제11권5호
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• pp.281-287
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• 2011

Background: Biodegradable polymers have increasingly been recognized for various biological applications in recent years. Here we examined the immunostimulatory activities of the novel poly(aspartic acid) conjugated with L-lysine (PLA). Methods: PLA was synthesized by conjugating L-lysine to aspartic acid polymer. PLA-nanoliposomes (PLA-NLs) were prepared from PLA using a microfluidizer. The immunostimulatory activities of PLA-NLs were examined in mouse bone marrow-derived dendritic cells (BM-DCs). Results: PLA-NLs increased the number of BM-DCs when added to cultures of GM-CSF-induced DC generation on day 4 after the initiation of cultures. Examination of the phenotypic properties showed that BM-DCs generated in the presence of PLA-NLs are more mature in terms of the expression of MHC class II molecules and major co-stimulatory molecules than BM-DCs generated in the absence of PLA-NLs. In addition, the BM-DCs exhibited enhanced capability to produce cytokines, such as IL-6, IL-12, TNF-${\alpha}$ and IL-$1{\beta}$. Allogeneic mixed lymphocyte reactions also confirmed that the BMDCs were more stimulatory on allogeneic T cells. PLA- NL also induced further growth of immature BM-DCs that were harvested on day 8. Conclusion: These results show that PLA-NLs induce the generation and functional activities of BM-DCs, and suggest that PLA-NLs could be immunostimulating agents that target DCs.

• 응용통계연구
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• 제26권3호
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• pp.431-440
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• 2013

우리는 두꺼운 꼬리를 갖는 분포의 모수를 추정하는 방법론을 연구하였다. 일반적으로 MLE(최대우도 추정량)가 모수추정 방법론중에 가장 많이 사용되는데, 이는 MLE가 점근적 일치성과 정규성 그리고 효율성을 가지고 있기 때문이다. 하지만 MLE가 늘 가장 좋은 추정법은 아니다. 어떤 경우에는 MLE가 존재하지 않을 수도 있고 계산이 안정적이지 않을 수도 있다. 본 논문에서는 비선형 최소제곱추정법을 이용한 모수추정 방법론을 제시하고 그 성능을 MLE와 비교하였다. NLS 추정량은 empirical CDF와 이론적 CDF의 차이의 제곱을 최소화 하는 방법론이다. 본 논문에서는 두꺼운 꼬리를 가지는 다양한 분포하에서 우리가 제안하는 NLS방법론과 MLE와의 성능을 비교하였다. 그 결과, Burr 분포에서 표본의 수가 적을 때 우리의 방법론이 MLE보다 좋은 성능을 보여주었고, Frechet 분포에서도 좋은 결과를 얻을 수 있었다.