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Nanoliposomes of L-lysine-conjugated poly(aspartic acid) Increase the Generation and Function of Bone Marrowderived Dendritic Cells

  • Received : 2011.09.19
  • Accepted : 2011.10.04
  • Published : 2011.10.31
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Abstract

Background: Biodegradable polymers have increasingly been recognized for various biological applications in recent years. Here we examined the immunostimulatory activities of the novel poly(aspartic acid) conjugated with L-lysine (PLA). Methods: PLA was synthesized by conjugating L-lysine to aspartic acid polymer. PLA-nanoliposomes (PLA-NLs) were prepared from PLA using a microfluidizer. The immunostimulatory activities of PLA-NLs were examined in mouse bone marrow-derived dendritic cells (BM-DCs). Results: PLA-NLs increased the number of BM-DCs when added to cultures of GM-CSF-induced DC generation on day 4 after the initiation of cultures. Examination of the phenotypic properties showed that BM-DCs generated in the presence of PLA-NLs are more mature in terms of the expression of MHC class II molecules and major co-stimulatory molecules than BM-DCs generated in the absence of PLA-NLs. In addition, the BM-DCs exhibited enhanced capability to produce cytokines, such as IL-6, IL-12, TNF-${\alpha}$ and IL-$1{\beta}$. Allogeneic mixed lymphocyte reactions also confirmed that the BMDCs were more stimulatory on allogeneic T cells. PLA- NL also induced further growth of immature BM-DCs that were harvested on day 8. Conclusion: These results show that PLA-NLs induce the generation and functional activities of BM-DCs, and suggest that PLA-NLs could be immunostimulating agents that target DCs.

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References

  1. Min SK, Kim SH, Kim JH: Preparation and swelling behavior of biodegradable poly(aspartic acid)-based hydrogel. J Ind Eng Chem 6;276-279, 2000.
  2. Goddard JM, Hotchkiss JH: Polymer surface modification for the attachment of bioactive compounds. Progress in Polymer Science 32;698-725, 2007. https://doi.org/10.1016/j.progpolymsci.2007.04.002
  3. Champion JA, Walker A, Mitragotri S: Role of particle size in phagocytosis of polymeric microspheres. Pharm Res 25; 1815-1821, 2008. https://doi.org/10.1007/s11095-008-9562-y
  4. Elamanchili P, Diwan M, Cao M, Samuel J: Characterization of poly(D,L-lactic-co-glycolic acid) based nanoparticulate system for enhanced delivery of antigens to dendritic cells. Vaccine 22;2406-2412, 2004. https://doi.org/10.1016/j.vaccine.2003.12.032
  5. Akagi T, Shima F, Akashi M: Intracellular degradation and distribution of protein-encapsulated amphiphilic poly(amino acid) nanoparticles. Biomaterials 32;4959-4967, 2011. https://doi.org/10.1016/j.biomaterials.2011.03.049
  6. Foged C, Sundblad A, Hovgaard L: Targeting vaccines to dendritic cells. Pharm Res 19;229-238, 2002. https://doi.org/10.1023/A:1014474414097
  7. Panyam J, Labhasetwar V: Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev 55;329-347, 2003. https://doi.org/10.1016/S0169-409X(02)00228-4
  8. Matsusaki M, Hiwatari K, Higashi M, Kaneko T, Akashi M: Stably-dispersed and surface-functional bionanoparticles prepared by self-assembling amphipaethic polymers of hydrophilic poly(g-glutamic acid) bearing hydrophobic amino acids. Chemistry Letters 33;398-399, 2004. https://doi.org/10.1246/cl.2004.398
  9. Akagi T, Wang X, Uto T, Baba M, Akashi M: Protein direct delivery to dendritic cells using nanoparticles based on amphiphilic poly(amino acid) derivatives. Biomaterials 28; 3427-3436, 2007. https://doi.org/10.1016/j.biomaterials.2007.04.023
  10. Giammona G, Pitarresi G, Tomarchio V, Dispenza C, Spadaro G: Synthesis and characterization of water-swellable ${\alpha}$,${\beta}$-polyasparthydrazide derivatives. II. Hydrogels at low crosslinking degree as potential systems for anticancer drug release. Colloid and Polymer Science 273; 559-564, 1995. https://doi.org/10.1007/BF00658685
  11. Nakato T, Yoshitake M, Matsubara K, Tomida M, Kakuchi T: Relationships between structure and properties of poly(aspartic acid)s. Macromolecules 31;2107-2113, 1998. https://doi.org/10.1021/ma971629y
  12. Nakato1 T, Kusuno A, Kakuchi T: Synthesis of poly(succinimide) by bulk polycondensation of L-aspartic acid with an acid catalyst. Journal of Polymer Science Part A: Polymer Chemistry 38;117-122, 2000. https://doi.org/10.1002/(SICI)1099-0518(20000101)38:1<117::AID-POLA15>3.0.CO;2-F
  13. Matsubara K, Nakato T, Tomida M: 1H and 13C NMR characterization of poly(succinimide) prepared by thermal polycondensation of l-aspartic acid. Macromolecules 30; 2305-2312, 1997. https://doi.org/10.1021/ma961579h
  14. Horgan A, Saunders B, Vincent B, Heenan RK: Poly(butyl methacrylate-g-methoxypoly(ethylene glycol)) and poly (methyl methacrylate-g-methoxypoly(ethylene glycol)) graft copolymers: preparation and aqueous solution properties. J Colloid Interface Sci 262;548-559, 2003. https://doi.org/10.1016/S0021-9797(03)00239-X
  15. Zhu G: Micellization of polystyrene-graft-poly(ethylene oxide) and its mixtures with polystyrene homopolymer in ethanol. European Polymer Journal 41;2671-2677, 2005. https://doi.org/10.1016/j.eurpolymj.2005.05.023
  16. Li P, Yin YL, Li D, Kim SW, Wu G: Amino acids and immune function. Br J Nutr 98;237-252, 2007. https://doi.org/10.1017/S000711450769936X
  17. Kidd MT, Kerr BJ, Anthony NB: Dietary interactions between lysine and threonine in broilers. Poult Sci 76;608-614, 1997. https://doi.org/10.1093/ps/76.4.608
  18. Konashi S, Takahashi K, Akiba Y: Effects of dietary essential amino acid deficiencies on immunological variables in broiler chickens. Br J Nutr 83;449-456, 2000.
  19. Yang SR, Lee HJ, Kim JD: Histidine-conjugated poly(amino acid) derivatives for the novel endosomolytic delivery carrier of doxorubicin. J Control Release 114;60-68, 2006. https://doi.org/10.1016/j.jconrel.2006.05.016
  20. Xu Q, An L, Yu M, Wang S: Design and synthesis of a new conjugated polyelectrolyte as a reversible ph sensor. Macromolecular Rapid Communications 29;390-395, 2008. https://doi.org/10.1002/marc.200700745
  21. Jeong JH, Park TG: Poly(L-lysine)-g-poly(D,L-lactic-co-glycolic acid) micelles for low cytotoxic biodegradable gene delivery carriers. J Control Release 82;159-166, 2002. https://doi.org/10.1016/S0168-3659(02)00131-1
  22. Stone WL, Mukherjee S, Smith M, Das SK: Therapeutic uses of antioxidant liposomes. Methods Mol Biol 199;145-161, 2002.
  23. Stone WL, Smith M: Therapeutic uses of antioxidant liposomes. Mol Biotechnol 27;217-230, 2004. https://doi.org/10.1385/MB:27:3:217
  24. Chen CH, Liu DZ, Fang HW, Liang HJ, Yang TS, Lin SY: Evaluation of multi-target and single-target liposomal drugs for the treatment of gastric cancer. Biosci Biotechnol Biochem 72;1586-1594, 2008. https://doi.org/10.1271/bbb.80096
  25. Koike M, Ishino K, Kohno Y, Tachikawa T, Kartasova T, Kuroki T, Huh N: DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes. Cancer Lett 108;185-193, 1996. https://doi.org/10.1016/S0304-3835(96)04408-4
  26. Paromov V, Kumari S, Brannon M, Kanaparthy NS, Yang H, Smith MG, Stone WL: Protective effect of liposome- encapsulated glutathione in a human epidermal model exposed to a mustard gas analog. J Toxicol 2011;109516, 2011.
  27. Kim SI, Min SK, Kim JH: Synthesis and characterization of novel amino acid-conjugated poly(aspartic acid) derivatives. Bull Korean Chem Soc 29;1887-1892, 2008 https://doi.org/10.5012/bkcs.2008.29.10.1887
  28. Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK: Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells. Int Immunopharmacol 1;1275-1284, 2001. https://doi.org/10.1016/S1567-5769(01)00052-2
  29. Lee YR, Lee YH, Im SA, Kim K, Lee CK: Formulation and characterization of antigen-loaded plga nanoparticles for efficient cross-priming of the antigen. Immune Netw 11; 163-168, 2011. https://doi.org/10.4110/in.2011.11.3.163
  30. Im SA, Lee YR, Lee YH, Oh ST, Gerelchuluun T, Kim BH, Kim Y, Yun YP, Song S, Lee CK: Synergistic activation of monocytes by polysaccharides isolated from Salicornia herbacea and interferon-gamma. J Ethnopharmacol 111;365-370, 2007. https://doi.org/10.1016/j.jep.2006.11.027
  31. Lee YH, Lee YR, Kim KH, Im SA, Song S, Lee MK, Kim Y, Hong JT, Kim K, Lee CK: Baccatin III, a synthetic precursor of taxol, enhances MHC-restricted antigen presentation in dendritic cells. Int Immunopharmacol 11; 985-991, 2011. https://doi.org/10.1016/j.intimp.2011.02.013
  32. Talmor M, Mirza A, Turley S, Mellman I, Hoffman LA, Steinman RM: Generation or large numbers of immature and mature dendritic cells from rat bone marrow cultures. Eur J Immunol 28;811-817, 1998. https://doi.org/10.1002/(SICI)1521-4141(199803)28:03<811::AID-IMMU811>3.0.CO;2-S
  33. Mora JR: Homing imprinting and immunomodulation in the gut: role of dendritic cells and retinoids. Inflamm Bowel Dis 14;275-289, 2008. https://doi.org/10.1002/ibd.20280
  34. Cai Z, Brunmark AB, Luxembourg AT, Garcia KC, Degano M, Teyton L, Wilson I, Peterson PA, Sprent J, Jackson MR: Probing the activation requirements for naive CD8+ T cells with Drosophila cell transfectants as antigen presenting cells. Immunol Rev 165;249-265, 1998. https://doi.org/10.1111/j.1600-065X.1998.tb01243.x
  35. Drutman SB, Trombetta ES: Dendritic cells continue to capture and present antigens after maturation in vivo. J Immunol 185;2140-2146, 2010. https://doi.org/10.4049/jimmunol.1000642
  36. Karlsen M, Hovden AO, Vogelsang P, Tysnes BB, Appel S: Bromelain treatment leads to maturation of monocyte- derived dendritic cells but cannot replace PGE2 in a cocktail of IL-1${\beta}$, IL-6, TNF-${\alpha}$ and PGE2. Scand J Immunol 74;135-143, 2011. https://doi.org/10.1111/j.1365-3083.2011.02562.x

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