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http://dx.doi.org/10.4110/in.2011.11.5.281

Nanoliposomes of L-lysine-conjugated poly(aspartic acid) Increase the Generation and Function of Bone Marrowderived Dendritic Cells  

Im, Sun-A (College of Pharmacy, Chungbuk National University)
Kim, Ki-Hyang (College of Pharmacy, Chungbuk National University)
Ji, Hong-Geun (H&A PharmaChem, R&D Center)
Yu, Hyoung-Gyoung (H&A PharmaChem, R&D Center)
Park, Sun-Ki (Cosmeca, R&D Center)
Lee, Chong-Kil (College of Pharmacy, Chungbuk National University)
Publication Information
IMMUNE NETWORK / v.11, no.5, 2011 , pp. 281-287 More about this Journal
Abstract
Background: Biodegradable polymers have increasingly been recognized for various biological applications in recent years. Here we examined the immunostimulatory activities of the novel poly(aspartic acid) conjugated with L-lysine (PLA). Methods: PLA was synthesized by conjugating L-lysine to aspartic acid polymer. PLA-nanoliposomes (PLA-NLs) were prepared from PLA using a microfluidizer. The immunostimulatory activities of PLA-NLs were examined in mouse bone marrow-derived dendritic cells (BM-DCs). Results: PLA-NLs increased the number of BM-DCs when added to cultures of GM-CSF-induced DC generation on day 4 after the initiation of cultures. Examination of the phenotypic properties showed that BM-DCs generated in the presence of PLA-NLs are more mature in terms of the expression of MHC class II molecules and major co-stimulatory molecules than BM-DCs generated in the absence of PLA-NLs. In addition, the BM-DCs exhibited enhanced capability to produce cytokines, such as IL-6, IL-12, TNF-${\alpha}$ and IL-$1{\beta}$. Allogeneic mixed lymphocyte reactions also confirmed that the BMDCs were more stimulatory on allogeneic T cells. PLA- NL also induced further growth of immature BM-DCs that were harvested on day 8. Conclusion: These results show that PLA-NLs induce the generation and functional activities of BM-DCs, and suggest that PLA-NLs could be immunostimulating agents that target DCs.
Keywords
Nanoliposome; Poly(aspartic acid); L-Lysine; Dendritic cell; Immunomodulation;
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