• ALGAE
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• v.27 no.2
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• pp.75-82
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• 2012

$Arthrospira$ $platensis$ and $Arthrospira$ $maxima$ are species of cyanobacteria used in health foods, animal feed, food additives, and fine chemicals. This study conducted a comparison of the 16S rRNA gene and $cpcBA$-intergenic spacer ($cpcBA$-IGS) sequences in $Arthrospira$ strains from culture collections around the world. A cluster analysis divided the 10 $Arthrospira$ strains into two main genotypic clusters, designated I and II, where Group I contained $A.$ $platensis$ SAG 86.79, UTEX 2340, $A.$ $maxima$ KCTC AG30054, and SAG 49.88, while Group II contained $A.$ $platensis$ PCC 9108, NIES 39, NIES 46, and SAG 257.80. However, although $A.$ $platensis$ PCC 9223 belonged to Group II-2 based on its $cpcBA$-IGS sequence, this strain also belonged to Group I based on its 16S rRNA gene sequence. Phylogenetic analyses based on the 16S rRNA gene and $cpcBA$-IGS sequences showed no division between $A.$ $platensis$ and $A.$ $maxima$, plus the 16S rRNA gene and $cpcBA$-IGS sequence clusters did not indicate any well-defined geographical distribution, instead overlapping in a rather interesting way. Therefore, the current study supports some previous conclusions based on 16S rRNA gene and $cpcBA$-IGS sequences, which found that $Arthrospira$ taxa are monophyletic. However, when compared with 16S rRNA sequences, $cpcBA$-IGS sequences may be better suited to resolve close relationships and intraspecies variability.

• Korean Journal of Environmental Agriculture
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• v.11 no.1
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• pp.20-25
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• 1992

To understand the fundamental features of reservoir environment and its future aspects, a simple predictive model for water quality was attempted with the aid of data easily obtained, Based on the data from 12 reservoirs in Korea, application of the simple predictive model was successfully made by means of statistical methods and simple physical submodels. Significant information on th effects of retention time on primary production in a reservoir, longitudinal change in water quality affected by certain non-dimensional parameters were also obtained. The chlorophyll-a concentration can be predicted by the equation as ; chlorophyll-a=($395{\time}limiting$ nutrient concentration) - 1.090.

• Journal of The Korean Association of Information Education
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• v.13 no.4
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• pp.433-441
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• 2009

In this research, we are looking for new ways to parents' participation in the school education through the educational information. The qualitative research about this research is not at all state. We use the method of study based on the grounded theory. The case analysis object is a outstanding school of the educational information in 2007, ten excellent schools of the cyber home service, two research schools 2 and two schools of research for NEIS. The parents make the learning check on situation of the students and communicate with school through the cyber home learning service and NIES. Also, It is bringing parents around the school closer through the SMS service and is communication path out of time and space. We make the things that make communications work through it. We know it is building trust with the parent and school.

• Journal of Crop Science and Biotechnology
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• v.10 no.2
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• pp.112-116
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• 2007

Kinetin(KN) is an inducer of rice(Oryza sativa L.) defense/stress responses, as evidenced by the induction of inducible secondary metabolite and defense/stress protein markers in leaf. We show a novel light-dependent effect of KN-triggered defense stress responses in rice leaf. Leaf segments treated with KN(100 ${\mu}M$) show hypersensitive-like necrotic lesion formation only under continuous light illumination. Potent accumulation of two phytoalexins, sakuranetin and momilactone A(MoA) by KN that peaks at 48 h after treatment under continuous light is completely suppressed by incubation under continuous dark. Using two-dimensional gel electrophoresis we identified KN-induced changes in ribulose-1, 5-bisphosphate carboxylase/oxygenase, energy- and pathogenesis-related proteins(OsPR class 5 and 10 members) by N-terminal amino acid sequencing and mass spectrometry. These changes were light-inducible and could not be observed in the dark(and control). Present results provide a new dimension(light modulation/regulation) to our finding that KN has a potential role in the rice plant self-defense mechanism.

• International Area Studies Review
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• v.13 no.1
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• pp.309-340
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• 2009

Multinational Corporations (MNCs) are increasingly establishing globally-distributed R&D (Research and Development) centers in which sites around the world work collaboratively to develop new manufacturing and product technologies. Following new globalization strategies, these projects more often include sites in Emerging/Newly Industrializing Economies (E/NIEs). The success of these ventures promises to be increasingly important both for the bottom line of MNCs and for the development ambitions of E/NIEs. This study attempts to identify these trends and to compare R&D transfer between Korea and China. The study identifies specific factors on MNC's R&D transfer and compares those operating factors between the R&D centers in Korea and China, which attract many MNCs in the world. Among those eight dimensions relating to MNCs' operation, there are significant differences in site selection, market characteristics, government support, and R&D networking between Korea and China. The analysis show more positive factors on China than Korea regarding R&D operations. The result will be helpful for both MNC's managers and governments' decision makers with respect to R&D transfer.

• Convergence Security Journal
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• v.4 no.3
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• pp.9-18
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• 2004

This paper is to analyze security and technical problem of NEIS and suggest an improving methods about the effective system. Therefore questionnaire was performed in the five items with security as well as technical problem of NEIS to primary and secondary school teachers. The result of questionnaire analysis on each item is that they think of NEIS as the essential system for the age of information, despite problems such as the invasion of human right and security as well as reveal of personal data.

• Journal of Microbiology and Biotechnology
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• v.3 no.1
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• pp.19-23
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• 1993

Spirulina platensis NIES 39 was grown in effluents from a wastewater treatment plant utilizing activated sludge process at a pig farm to reduce pollutants and to produce feed resources. The addition of 2 g/l NaCl was required for the growth of S. platensis in the effluents with about 100 mg/l ammonia nitrogen concentration. The growth was stimulated by the addition of 2 g/l sodium bicarbonate and 4 mg/l ferrous sulfate. The microorganism grew well at pH 8~11, and the optimum pH for the growth was 10. The algal concentration of 1.1 g/l was attained after 8 days of growth at the optimum condition with the removal of 95% ammonia nitrogen and 34% phosphorus.

• ALGAE
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• v.18 no.3
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• pp.233-238
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• 2003

Cyanobacteria were dominant from June to September in the Nakdong River and the Hoedong Reservoir. Microcystis aeruginosa was dominant from June to September; Anabaena flos-aquae from June to August and Aphanizomenon flos-aquae from July to August. Cyanobacterial neurotoxins, Anatoxin-a and saxitoxin were analyzed by electrospray ionization-mass spectrometry with strains of Aphanizomenon flos-aquae NIES-81 and Anabaena flosaquae NIER-10002. Anatoxin-a was not detected from the cultured Anabaena flos-aquae nor from the wild samples. Low levels of saxitoxin were detected in the cultured Aphanizomenon flos-aquae however, those of field samples were below the detection limit.

• ALGAE
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• v.20 no.1
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• pp.25-30
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• 2005

To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.135-141
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• 2000

Global Biodiversity is common interest of humans for better health and sustainable development of the society. To provide access and analysis on microbial diversity information, Bacteriology Insight Orienting System (BIOS) has been developed. BIOS contains 6402 species and subspecies names of bacteria and archaea, 2606 names of cyanobacteria by March 2000. BIOS of which web based analytical tool provides windows to compare the results of phylogenetic analysis based on 16S rDNA sequence and the results of cluster analysis on proteome profiling. The sequence data and 2 dimensional gel electrophoresis analysis data were accumulated in BIOS database content for cyanobacteria reclassification and taxonomy. (BIOS URL: http.://www-sp2000ao.nies.go.jp/bios/index.html).